FastQCFastQC Report
Wed 25 May 2016
SRR1295272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569398
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48370.13551304729817185No Hit
GTGGTATCAACGCAGAGTACATGGG46670.13075033941297665No Hit
GGTATCAACGCAGAGTACTTTTTTT39490.110634902580211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG658.016921E-411.6942154
AGGACCG907.454495E-611.6130045
GTCTACG909.453808E-510.5630511
CGTATAC800.0045117989.501553
ACGTCTA800.0045117989.501555
TATACCG1401.0572967E-69.5015495
CGTCTAT800.0045222429.4988866
CAGGACT4700.09.2993894
AAGACGG1151.1093898E-49.0884385
GCGTCAG1401.0762675E-58.8276931
GGTATCA34250.08.8267011
ATACCGT1201.7671223E-48.7073126
TACAGCG1107.156394E-48.6353517
TGAACCG1000.00290202688.5513945
CTACACG1356.789493E-58.4458214
GCAGGAC3700.08.2175563
TAGACAG5100.08.1974145
GTATTGG4550.08.148641
TTGGACG1050.00450819368.1441864
TGCGCTG1050.00450819368.1441865
Produced by FastQC (version 0.11.5)