##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295272_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3569398
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.167097925196348	31.0	30.0	33.0	26.0	34.0
2	30.154638401209393	31.0	30.0	34.0	26.0	34.0
3	30.480863159557998	31.0	30.0	34.0	26.0	34.0
4	33.86617687352321	35.0	35.0	37.0	28.0	37.0
5	34.026319844410736	35.0	35.0	37.0	28.0	37.0
6	34.280234370053435	36.0	35.0	37.0	30.0	37.0
7	34.24184918577306	37.0	35.0	37.0	30.0	37.0
8	34.323594903118114	37.0	35.0	37.0	30.0	37.0
9	35.29531394369583	39.0	35.0	39.0	28.0	39.0
10	35.23893608950305	39.0	35.0	39.0	27.0	39.0
11	35.61351886228434	39.0	35.0	39.0	29.0	39.0
12	35.617427644661646	39.0	35.0	39.0	29.0	39.0
13	35.523751904382756	39.0	35.0	39.0	29.0	39.0
14	35.73309140645005	39.0	34.0	41.0	26.0	41.0
15	36.34850890822486	39.0	36.0	41.0	27.0	41.0
16	36.44525939668258	39.0	36.0	41.0	28.0	41.0
17	36.25534894119401	39.0	36.0	41.0	27.0	41.0
18	36.22916889626766	39.0	36.0	41.0	27.0	41.0
19	36.30156457755621	39.0	36.0	41.0	27.0	41.0
20	36.27261571839285	39.0	36.0	41.0	27.0	41.0
21	36.26893806742762	39.0	35.0	41.0	27.0	41.0
22	36.011649863646475	39.0	35.0	41.0	27.0	41.0
23	35.69419941401883	39.0	34.0	41.0	26.0	41.0
24	36.022395653272625	39.0	34.0	41.0	27.0	41.0
25	34.76808862446833	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29687419285602346
1101	2	0.6213741716670214
1101	3	0.32542886541386196
1101	4	0.2666025122545008
1101	5	0.20225746707239267
1101	6	0.241219310356243
1101	7	0.16525958738937874
1101	8	0.23407636812722643
1101	9	0.21605541566144382
1101	10	0.4754228945041419
1101	11	0.46037363265990194
1101	12	0.4110409512555435
1101	13	0.3306402235858741
1101	14	0.9091468647048728
1101	15	0.643629315406713
1101	16	0.49582793939211456
1101	17	0.7598384354807237
1101	18	0.7808217019659764
1101	19	0.6648053526167743
1101	20	0.7190870179188877
1101	21	0.8447584121283782
1101	22	0.5643509812341705
1101	23	-1.7781195334561772
1101	24	0.06230257224540736
1101	25	0.3851865622122759
1102	1	0.2102326453408203
1102	2	0.3390387531892358
1102	3	0.22107700463772062
1102	4	0.43215663928145887
1102	5	0.1695942194334421
1102	6	0.2480981056155187
1102	7	0.20068014850077276
1102	8	0.25960401895325447
1102	9	0.496982448338791
1102	10	0.931767170019107
1102	11	0.5475158320069156
1102	12	0.5054717425336719
1102	13	0.4654567221634096
1102	14	0.9941158007762283
1102	15	0.7263915961511813
1102	16	0.5203282204773103
1102	17	1.0031346868484974
1102	18	0.8922056147615081
1102	19	0.8250491950456649
1102	20	0.8933160466396188
1102	21	0.7875820956549333
1102	22	0.6754198771467657
1102	23	0.7525654559075861
1102	24	0.8443556627288089
1102	25	0.6326428221409799
1103	1	-0.290683611891172
1103	2	-1.4967273615189782
1103	3	-0.3716021536443783
1103	4	-0.16360119863951894
1103	5	-0.2570963335142622
1103	6	-0.0880220393368134
1103	7	-0.07311048879694226
1103	8	0.17672532596196788
1103	9	0.7277662507243576
1103	10	0.7918966670457195
1103	11	0.5168048434970061
1103	12	0.4649943437464117
1103	13	0.2193275888159647
1103	14	-0.8710322700675661
1103	15	-0.09392939931091604
1103	16	0.024244724395309447
1103	17	0.43438885991791665
1103	18	0.5208799290017367
1103	19	0.6153652404696643
1103	20	0.5493475690952465
1103	21	0.7120721980973812
1103	22	0.2003576185515854
1103	23	1.082443033100546
1103	24	0.8963800168878961
1103	25	0.713444797842584
1104	1	-0.018530077273510415
1104	2	0.1550862358955314
1104	3	-0.2746873573976991
1104	4	-0.07995879010341156
1104	5	-0.15986814933049942
1104	6	-0.05940567910249683
1104	7	-0.03463323025601994
1104	8	0.004175782951072904
1104	9	-2.0082175529276824
1104	10	-0.4588650305436843
1104	11	-0.351888518737411
1104	12	0.14228571053080685
1104	13	0.1495403576554395
1104	14	-1.4795481253107496
1104	15	-0.3443092082642707
1104	16	-0.04014858283979095
1104	17	0.18992779109941438
1104	18	0.4639911843696183
1104	19	0.4776342972279224
1104	20	0.6527752321731057
1104	21	0.7176079821484294
1104	22	0.8072001206108368
1104	23	1.187100837693265
1104	24	0.8690944327256389
1104	25	0.5811905241314577
1105	1	-0.29251169722105175
1105	2	-0.13062964895656393
1105	3	-1.4879533869272343
1105	4	-0.7067490906460492
1105	5	-0.536507395159191
1105	6	-0.3336414786193913
1105	7	-0.3122204736284573
1105	8	-0.16396161388624364
1105	9	0.0684514061316861
1105	10	0.2660209359547139
1105	11	-0.22629139638121387
1105	12	-0.20044369819850516
1105	13	-0.2916159781668526
1105	14	0.5206347702164109
1105	15	0.09384798365931601
1105	16	-0.377457305056744
1105	17	0.11654573740214857
1105	18	0.02230614337508996
1105	19	0.03974736480341079
1105	20	-0.41295485333529314
1105	21	-0.025684362678333628
1105	22	0.07125294912334823
1105	23	0.28836669278655336
1105	24	0.07975905049568155
1105	25	0.37179953889010164
1106	1	-1.5450853784465046
1106	2	-1.0183045568423168
1106	3	-1.2130448887660954
1106	4	-1.4179250365860838
1106	5	-1.0712102051076045
1106	6	-0.8455996407349033
1106	7	-0.8207148958468622
1106	8	-0.7821175497078201
1106	9	-0.4169411562272174
1106	10	-1.9114628369053364
1106	11	-1.3395687852990932
1106	12	-0.9903289733474594
1106	13	-1.0218159020749695
1106	14	-0.27116081973399275
1106	15	-1.0822306719631882
1106	16	-1.547593291799366
1106	17	-0.8744504325000761
1106	18	-0.7544004818040548
1106	19	-0.6166598710890412
1106	20	-0.32606703672712456
1106	21	-0.3984638392881763
1106	22	-0.4583337030566099
1106	23	-0.6568235415539618
1106	24	-0.986302692551547
1106	25	-1.5838808273372393
1107	1	-0.07111112820137677
1107	2	0.15160685612595515
1107	3	-0.0436986179907386
1107	4	0.08053712240023714
1107	5	-0.18179727588179162
1107	6	0.009549603905455228
1107	7	-0.12183780962737956
1107	8	-0.22036137615138784
1107	9	0.34132918191269823
1107	10	0.005702500985208303
1107	11	-0.017635733702498158
1107	12	-0.11226404090015762
1107	13	0.002346980583382674
1107	14	-1.1541319448349796
1107	15	-0.5484053079454014
1107	16	-0.30458331045595344
1107	17	-0.15449648772925428
1107	18	0.26073144210117505
1107	19	0.12921447286806398
1107	20	0.2925170658702001
1107	21	0.23553374658565218
1107	22	-0.37199288859011403
1107	23	0.4730576530566637
1107	24	0.30549893481965285
1107	25	0.03473321483124181
1108	1	-0.11614519823139702
1108	2	0.19233674012275515
1108	3	-0.04105194629107345
1108	4	-0.19890459186758136
1108	5	-0.3408734704611689
1108	6	-0.21185964965516746
1108	7	-0.1640172395446129
1108	8	-0.18739631514883826
1108	9	0.32258502532342703
1108	10	0.3368133593991587
1108	11	0.03611315538436344
1108	12	0.04063622152313684
1108	13	-0.10397340448655257
1108	14	0.07936121607510671
1108	15	-0.009857420025781494
1108	16	-0.021882755478252136
1108	17	0.28279270481514374
1108	18	0.3136235864592223
1108	19	0.2800883810719057
1108	20	0.3812948288172251
1108	21	0.21751598961012064
1108	22	0.31593937938144734
1108	23	0.10406553062042434
1108	24	0.1643263578264893
1108	25	-0.6250314342479086
1109	1	-0.9065584431427212
1109	2	-0.8058130578958256
1109	3	-0.9217242940346111
1109	4	-0.7824354333304981
1109	5	-0.9602720119204804
1109	6	-1.1849651807526058
1109	7	-0.9379145427727096
1109	8	-1.2439192401330246
1109	9	-0.9617128609233205
1109	10	-1.1765197129923237
1109	11	-1.1936678895510653
1109	12	-1.203430457817845
1109	13	-1.2956312177689924
1109	14	-0.9155529651204262
1109	15	-1.3530633640326357
1109	16	-1.4597707666912996
1109	17	-1.501552150964784
1109	18	-2.048884785425159
1109	19	-2.3029961876968343
1109	20	-2.038561789848572
1109	21	-1.7455102275904224
1109	22	-2.029536199567751
1109	23	-1.2609680128332243
1109	24	-2.2070883943346686
1109	25	-1.3498671340753106
1110	1	0.10221670642032521
1110	2	0.23478994958758648
1110	3	-0.01588672241090805
1110	4	0.3720102205798099
1110	5	0.29538612120084196
1110	6	0.2266521929562515
1110	7	0.12334302516067197
1110	8	0.15018535678667178
1110	9	0.5153547994041787
1110	10	0.3980281923820357
1110	11	0.31422059356821563
1110	12	0.32332191581051717
1110	13	0.27014494353495166
1110	14	-2.322533543905571
1110	15	-0.530363402793327
1110	16	-0.027853375273885206
1110	17	-0.07570508111255947
1110	18	-2.539514727259345
1110	19	-0.3119123246546067
1110	20	-1.8500466589352413
1110	21	-0.18959507265289233
1110	22	-1.5930088856593514
1110	23	-0.40899301286418677
1110	24	0.15466687086652797
1110	25	0.05209145761107692
1111	1	-0.2226734002091746
1111	2	-1.44453663599608
1111	3	-0.43198217513396386
1111	4	0.07600213254353605
1111	5	-0.04822935259626604
1111	6	0.09952846829106932
1111	7	-0.06438177318118932
1111	8	-0.019849684616026764
1111	9	-1.6330592188937771
1111	10	-0.3956911319166636
1111	11	-0.20596070697296653
1111	12	-0.14858026353025622
1111	13	2.2279884478848544E-4
1111	14	0.8762989882953249
1111	15	0.3358706378813281
1111	16	0.2776981034559398
1111	17	-0.05742247169320791
1111	18	0.238558994293939
1111	19	-0.01659174864978752
1111	20	0.31725396619412294
1111	21	-0.3967259474432012
1111	22	-0.13543720710234197
1111	23	0.09093317005454082
1111	24	-0.43005154591629235
1111	25	-1.90999536575044
1112	1	0.2570421678412558
1112	2	0.43089683421687397
1112	3	0.09364338209191558
1112	4	0.47664423029424086
1112	5	0.2678137633999924
1112	6	0.1677066677708794
1112	7	0.18291913855423303
1112	8	0.07582591718792031
1112	9	-0.23818385310413248
1112	10	0.5376096626793014
1112	11	0.2296358040066906
1112	12	0.1011575827090283
1112	13	0.10356626257168955
1112	14	0.46948841363953164
1112	15	0.13628756756945393
1112	16	0.1718053381188085
1112	17	-3.5421759039876903
1112	18	-0.8006649351273722
1112	19	-1.0493187768435206
1112	20	-0.05992683421543177
1112	21	-0.4383542726310026
1112	22	-1.5921830296707782
1112	23	-1.2820063144017055
1112	24	-0.2585520090509874
1112	25	0.18524291542330218
1113	1	-0.18018955283915972
1113	2	-0.11059094374575551
1113	3	-0.02187695673156753
1113	4	-0.4924700250451224
1113	5	-0.11210272582884784
1113	6	-0.20609091425998827
1113	7	-0.21090821777917057
1113	8	-0.20825755575275906
1113	9	0.2548648403043785
1113	10	0.40066884974540784
1113	11	-0.05253705804491915
1113	12	-0.056982310084571
1113	13	-0.4303267038404357
1113	14	-1.3784175191675274
1113	15	-1.6365868649196997
1113	16	-0.5042292716061141
1113	17	-0.2121983916250727
1113	18	-0.5368431200986166
1113	19	-0.11982987944277568
1113	20	0.2525022390196341
1113	21	-1.536701983693881
1113	22	-2.0397673174712665
1113	23	-0.6430186578834878
1113	24	-2.200353693968708
1113	25	-0.5381465058865231
1114	1	0.22290982528611636
1114	2	0.23308713999968234
1114	3	-0.007117949173355953
1114	4	0.3496511001853264
1114	5	0.1539109353567838
1114	6	0.1936894203905979
1114	7	0.1784213536125705
1114	8	0.1648018201916841
1114	9	0.497732363285607
1114	10	0.3293227087139883
1114	11	0.19801664873633484
1114	12	0.20814025422820492
1114	13	0.16345512104788185
1114	14	1.0103982264662577
1114	15	0.5239726204030717
1114	16	0.46734607593091937
1114	17	-0.3708012668280958
1114	18	0.02235832383001224
1114	19	-0.32789767583618357
1114	20	-1.411655120392183
1114	21	-0.5492682080682201
1114	22	-1.223673944721348
1114	23	-2.83012174073707
1114	24	-1.9113686475014262
1114	25	-1.4434486795200812
1115	1	0.5096468751010903
1115	2	0.4772742450737866
1115	3	0.3772966237666111
1115	4	0.5930618688268012
1115	5	0.6488372370999969
1115	6	0.4197671273244623
1115	7	0.5428294336867836
1115	8	0.445588926056935
1115	9	0.7766857757376258
1115	10	0.9492581580468951
1115	11	0.6421046851394578
1115	12	0.5443118950959231
1115	13	0.5718446749729509
1115	14	1.2167457636713976
1115	15	0.6584118066774494
1115	16	0.6796489137824437
1115	17	0.7063779362668541
1115	18	0.8659953593384841
1115	19	0.301965270744752
1115	20	0.8665419264911591
1115	21	-0.2123198461845135
1115	22	0.828433061090692
1115	23	0.8046011581687083
1115	24	0.8397050817417835
1115	25	0.9887772130923764
1116	1	0.43742904390413173
1116	2	0.5145898991356468
1116	3	0.2855488904242911
1116	4	0.6832539176300756
1116	5	0.525973602883667
1116	6	0.3600950277884962
1116	7	0.3161683622391678
1116	8	0.2563660030817161
1116	9	0.5009629578799633
1116	10	0.4553997029074992
1116	11	0.32447991087975225
1116	12	0.3925748586042488
1116	13	-0.20558568844647596
1116	14	0.8746932999286656
1116	15	0.4835179671186083
1116	16	0.44940243591113926
1116	17	0.6737256339161704
1116	18	0.6627207472581702
1116	19	0.9346284578039885
1116	20	0.7037308593394158
1116	21	0.18808277752751934
1116	22	0.1881878457350794
1116	23	-0.7738124204223382
1116	24	-0.35426567375368023
1116	25	-1.0044098986391532
1201	1	0.33812801563931316
1201	2	0.5816208020022451
1201	3	0.4648588554503341
1201	4	0.41389136779360314
1201	5	0.3492784016617776
1201	6	0.23551003945792104
1201	7	0.2842315296792677
1201	8	0.41185609667891754
1201	9	0.5881520778358293
1201	10	0.8330601280692917
1201	11	0.5151617105416832
1201	12	0.5716196758349454
1201	13	0.5727536798373336
1201	14	1.0435696708514186
1201	15	0.7212182644435146
1201	16	0.5223890603197248
1201	17	0.6355809461401591
1201	18	0.43469235975853593
1201	19	0.8484051289171504
1201	20	0.7775721175064518
1201	21	0.7377905418537196
1201	22	0.8894899145326178
1201	23	0.47120664854862326
1201	24	0.7571990805137432
1201	25	0.892049633562884
1202	1	0.309638486677418
1202	2	0.7677562362720423
1202	3	0.509599573975219
1202	4	0.45089937895963317
1202	5	0.27163157354137013
1202	6	0.18351855240324966
1202	7	0.16192172998725596
1202	8	0.28169639244032396
1202	9	0.6641590681405347
1202	10	-0.005187912396038996
1202	11	0.3056200599761709
1202	12	0.35009853477728825
1202	13	0.33286626296255406
1202	14	1.0079442635162081
1202	15	0.7593334199085859
1202	16	0.5179724796861009
1202	17	-1.4326393522743288
1202	18	-0.008697361263713788
1202	19	0.4781386091314701
1202	20	0.6031621695876765
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2210	22	1.3112750173323704
2210	23	1.6326026223606789
2210	24	1.332156568225443
2210	25	1.2509867174534435
2211	1	0.12100931179674745
2211	2	-0.484285232043554
2211	3	-0.04694990973094093
2211	4	0.2546617316621749
2211	5	0.208174623383222
2211	6	0.090778962268935
2211	7	0.23280354972495587
2211	8	0.21530978375238874
2211	9	0.6241638222274659
2211	10	0.6308127595149173
2211	11	0.23211683670523087
2211	12	0.15188955779340318
2211	13	0.15200938633427796
2211	14	-0.9151999311097683
2211	15	-0.06706054176713394
2211	16	0.19029928829923648
2211	17	0.27943821540802105
2211	18	0.15331729400159588
2211	19	0.5032808197185048
2211	20	0.5526709773260663
2211	21	0.5788789922182076
2211	22	-0.10116786632973174
2211	23	0.3648925624353794
2211	24	-0.2570087829705585
2211	25	0.27359556897881276
2212	1	-0.01677323352341986
2212	2	-1.0920994496886038
2212	3	-0.35065335076013326
2212	4	0.08038555720122531
2212	5	0.13292905554901324
2212	6	0.08096348109035745
2212	7	0.11636226297377306
2212	8	0.058628593604588275
2212	9	-2.2710991401894134
2212	10	-0.05750109512086965
2212	11	0.060395491573942195
2212	12	-0.01242952454904156
2212	13	0.060040196395519274
2212	14	0.6183693271381969
2212	15	0.2871550782694854
2212	16	0.3648850471379035
2212	17	0.38070356864682964
2212	18	0.05373890733047659
2212	19	-0.6507883834714931
2212	20	0.219713795621729
2212	21	0.2199160228957595
2212	22	-1.7747520835455148
2212	23	-0.40096996250400707
2212	24	0.2024771903141982
2212	25	-0.37885945613321326
2213	1	0.1359539375669918
2213	2	0.41886208584110207
2213	3	0.1358708718449222
2213	4	0.34899081995792613
2213	5	0.4051792702568804
2213	6	0.22340881316339534
2213	7	0.23106454319675862
2213	8	0.125928712011536
2213	9	0.5940325557740778
2213	10	0.6341764975084203
2213	11	0.13015649249375372
2213	12	0.022999814963817755
2213	13	0.07738706775882065
2213	14	-1.6077739605093697
2213	15	-0.4801257291337464
2213	16	-0.1639721988080538
2213	17	-2.136701743911935
2213	18	-0.8615886936838564
2213	19	-0.47516814452323075
2213	20	0.21876158527408052
2213	21	-0.26034863949199405
2213	22	0.3446521719898783
2213	23	0.7904490913584468
2213	24	0.6587460248951302
2213	25	-0.9741205104123836
2214	1	0.3424407468260888
2214	2	0.20293088061511
2214	3	0.20771117653486826
2214	4	0.4460604377096047
2214	5	0.4483802941027051
2214	6	0.3600287531767066
2214	7	0.29456899086923727
2214	8	0.23484493083120128
2214	9	0.6361645641304676
2214	10	0.6495062959729978
2214	11	0.022589546425315632
2214	12	-0.020989624915181082
2214	13	-0.015567148333559544
2214	14	0.9435487066226784
2214	15	0.5273450602917649
2214	16	0.44229902794197073
2214	17	0.4208563906491918
2214	18	0.4184940489362887
2214	19	-0.738072554212124
2214	20	0.1379101028021381
2214	21	0.47087934243329244
2214	22	0.6654324437949413
2214	23	1.2043043738998591
2214	24	0.9057883526387158
2214	25	0.8314625632809083
2215	1	-0.1259998278246428
2215	2	0.18435916632225258
2215	3	0.21227066552209095
2215	4	0.3588794513667608
2215	5	0.3925966267837495
2215	6	0.2938196547674252
2215	7	0.22372318563319737
2215	8	0.18854718247840196
2215	9	0.26892799685264634
2215	10	0.6132926633594522
2215	11	0.3080210003934809
2215	12	0.18787576717416243
2215	13	0.0217091244823564
2215	14	0.9730165455913848
2215	15	0.42453635371045806
2215	16	0.3574223605707374
2215	17	0.6452897287553725
2215	18	0.32612696703196775
2215	19	-0.24780330884463098
2215	20	-1.642396109398618
2215	21	-1.8611558902657066
2215	22	-0.23353770091322446
2215	23	0.4156853970618002
2215	24	0.49504842504961744
2215	25	-0.11563014812778505
2216	1	-0.05346120293624068
2216	2	-0.34241848647493356
2216	3	-0.13839300665436838
2216	4	0.159840218669153
2216	5	0.27855295531345803
2216	6	0.0044640081946170085
2216	7	0.09713935083625103
2216	8	-0.06511606599703157
2216	9	0.20815338057950328
2216	10	-1.7093960223098676
2216	11	-0.6333821561791808
2216	12	-0.2931175847549383
2216	13	-0.029668408266203983
2216	14	-0.6270910189851975
2216	15	-0.4865847894752662
2216	16	-0.09802345866024353
2216	17	0.19639109922643883
2216	18	0.024951196677925225
2216	19	-1.4641701481862768
2216	20	-1.0110071770747737
2216	21	-0.9436401401887835
2216	22	-0.7374726981137698
2216	23	-4.578292625969379
2216	24	-2.2015652453614223
2216	25	-1.1457463380467274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	7.0
4	33.0
5	44.0
6	61.0
7	282.0
8	149.0
9	378.0
10	1061.0
11	2509.0
12	4861.0
13	8054.0
14	11930.0
15	15409.0
16	17939.0
17	20058.0
18	21207.0
19	21952.0
20	22724.0
21	23256.0
22	24639.0
23	26018.0
24	28309.0
25	31139.0
26	35604.0
27	41037.0
28	48017.0
29	59050.0
30	72966.0
31	91077.0
32	120462.0
33	160053.0
34	217053.0
35	302267.0
36	429965.0
37	644266.0
38	1065558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3154989335474	21.113266042281825	13.425778852470646	23.14545617170013
2	15.674803218005588	20.98619373604172	40.979748757940634	22.35925428801206
3	18.16864656603365	26.219614553682604	30.45182285353808	25.159916026745666
4	12.581179295678174	17.260626878488623	38.531620267553954	31.626573558279254
5	13.200284440079368	37.607614518104185	35.56451181163497	13.627589230181481
6	30.728898134490628	38.461223766974975	17.86827109738219	12.941607001152214
7	25.70796478775558	32.882844889306725	24.16071085804276	17.248479464894938
8	23.077343472145035	38.01300478605947	21.398228902956248	17.51142283883925
9	24.230619290899323	17.34271798104143	22.573069061743556	35.85359366631569
10	14.843927111154454	30.087203314953353	34.16874103454141	20.900128539350778
11	31.718235485822582	25.82432255589522	23.983711245623983	18.473730712658224
12	20.599825095613184	27.682135794958935	31.780928472736125	19.937110636691756
13	26.00729654757678	24.437353458854428	27.366733201869565	22.188616791699232
14	20.856436897840577	23.60591055136895	28.30023161486909	27.23742093592138
15	22.505725034128798	31.247686094087584	24.406876374855983	21.83971249692764
16	21.876204089764883	31.34140398653017	25.97566474161757	20.806727182087375
17	20.037757442200903	30.574981337051728	28.646588771742614	20.740672449004755
18	20.127749238663746	30.122853482950468	29.54780535910102	20.201591919284766
19	20.812099389846654	29.089197273574264	29.729921331549342	20.368782005029736
20	21.882544468230574	28.808464374978985	29.061319644478317	20.247671512312124
21	22.28855944432147	29.10237407431276	28.11183618803184	20.49723029333393
22	21.35434866002914	28.705771322138023	29.427771536782192	20.512108481050646
23	21.683554235435874	28.901000364278307	28.809118166279035	20.60632723400678
24	21.212550868779775	28.2796930346024	29.05624140278042	21.451514693837407
25	21.90324310498021	28.526966415666706	28.56353941357554	21.006251065777544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2814.0
1	2814.0
2	3147.5
3	3481.0
4	3481.0
5	3481.0
6	5299.0
7	7117.0
8	7117.0
9	7117.0
10	11467.0
11	15817.0
12	15817.0
13	15817.0
14	26483.5
15	37150.0
16	37150.0
17	37150.0
18	57378.5
19	77607.0
20	77607.0
21	77607.0
22	107960.5
23	138314.0
24	138314.0
25	138314.0
26	178332.0
27	218350.0
28	218350.0
29	218350.0
30	261584.0
31	304818.0
32	304818.0
33	304818.0
34	341640.0
35	378462.0
36	378462.0
37	378462.0
38	404253.5
39	430045.0
40	430045.0
41	430045.0
42	432600.0
43	435155.0
44	435155.0
45	435155.0
46	417560.0
47	399965.0
48	399965.0
49	399965.0
50	378671.0
51	357377.0
52	357377.0
53	357377.0
54	324554.5
55	291732.0
56	291732.0
57	291732.0
58	251082.5
59	210433.0
60	210433.0
61	210433.0
62	172659.0
63	134885.0
64	134885.0
65	134885.0
66	103568.5
67	72252.0
68	72252.0
69	72252.0
70	52539.5
71	32827.0
72	32827.0
73	32827.0
74	23176.0
75	13525.0
76	13525.0
77	13525.0
78	9237.5
79	4950.0
80	4950.0
81	4950.0
82	3304.0
83	1658.0
84	1658.0
85	1658.0
86	1082.0
87	506.0
88	506.0
89	506.0
90	315.0
91	124.0
92	124.0
93	124.0
94	74.0
95	24.0
96	24.0
97	24.0
98	17.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08127420926441939
2	0.02039559612013006
3	0.03134982425607904
4	0.03838182236892607
5	0.04726287177837832
6	0.017201780244175627
7	0.021095994338541122
8	0.03717713743325905
9	0.03644872328611155
10	0.023561396067348053
11	0.022440758917890356
12	0.016557413883237452
13	0.024121714642076898
14	0.039390395803438005
15	0.03633665957116578
16	0.023281236779983627
17	0.023253220851247185
18	0.028043944665178837
19	0.01745392360280361
20	0.015632888234934854
21	0.011990817499197342
22	0.019639166044246117
23	0.01955511825803679
24	0.01840646517984265
25	0.10999053621927282
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3569398.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.00738190580853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62236248821725	67.57183712201747
2	9.757937201734352	15.22382267817176
3	1.9286803098808627	4.513539045212687
4	0.6150557886889358	1.9191556720654437
5	0.306020193935956	1.1935917069625854
6	0.18673448269179838	0.8740000863793624
7	0.12178802052164737	0.6650255239869215
8	0.08362845690532181	0.5218909580805511
9	0.05609432581664189	0.3938194347054875
>10	0.2966016669007341	3.9823317310654986
>50	0.016626683719309202	0.8935077205636438
>100	0.007388215540326362	1.004495868932591
>500	6.492992813646278E-4	0.3409316964783484
>1k	4.328661655738147E-4	0.9020507553777245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4837	0.13551304729817185	No Hit
GTGGTATCAACGCAGAGTACATGGG	4667	0.13075033941297665	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3949	0.110634902580211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	2.801592873644239E-5	0.0	0.0	0.0
8	0.0	2.801592873644239E-5	0.0	0.0	0.0
9	0.0	2.801592873644239E-5	0.0	2.801592873644239E-5	0.0
10	0.0	2.801592873644239E-5	0.0	2.801592873644239E-5	0.0
11	0.0	1.9611150115509673E-4	0.0	2.801592873644239E-5	0.0
12	0.0	1.9611150115509673E-4	0.0	2.801592873644239E-5	0.0
13	0.0	1.9611150115509673E-4	0.0	5.603185747288478E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	65	8.016921E-4	11.694215	4
AGGACCG	90	7.454495E-6	11.613004	5
GTCTACG	90	9.453808E-5	10.563051	1
CGTATAC	80	0.004511798	9.50155	3
ACGTCTA	80	0.004511798	9.50155	5
TATACCG	140	1.0572967E-6	9.501549	5
CGTCTAT	80	0.004522242	9.498886	6
CAGGACT	470	0.0	9.299389	4
AAGACGG	115	1.1093898E-4	9.088438	5
GCGTCAG	140	1.0762675E-5	8.827693	1
GGTATCA	3425	0.0	8.826701	1
ATACCGT	120	1.7671223E-4	8.707312	6
TACAGCG	110	7.156394E-4	8.635351	7
TGAACCG	100	0.0029020268	8.551394	5
CTACACG	135	6.789493E-5	8.445821	4
GCAGGAC	370	0.0	8.217556	3
TAGACAG	510	0.0	8.197414	5
GTATTGG	455	0.0	8.14864	1
TTGGACG	105	0.0045081936	8.144186	4
TGCGCTG	105	0.0045081936	8.144186	5
>>END_MODULE