FastQCFastQC Report
Wed 25 May 2016
SRR1295272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569398
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125590.35185204900098No Hit
GGTATCAACGCAGAGTACTTTTTTT106700.2989299596178403No Hit
TATCAACGCAGAGTACTTTTTTTTT78660.22037329544085585No Hit
GTGGTATCAACGCAGAGTACATGGG53620.15022140988480412No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53540.14999728245491256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT909.4523995E-510.5632064
AGCGTCA3850.010.3711483
GTACGGA856.486316E-410.077421
GCAGCGT3500.010.0614241
GGTATCA43950.09.6799781
CAGCGTC4250.09.61873152
TAGACTG5350.09.5957365
TCTAGAC3400.09.50688653
GTTCTAG4150.09.4028941
CTAGACT4000.09.2692144
TGGTCGA850.0074612278.937880510
GCATGCG1304.3907476E-58.7659989
CCGTTCA1201.7719466E-48.7051239
ATAGACG1107.096711E-48.6426233
CTAGCAC3200.08.6156153
TAGTACG1000.00288731378.5561974
CGACTGT1000.00291528938.5470886
GTGTTAG5150.08.5011241
GTCTCGC1356.657113E-58.4600561
CCGCCCG1356.8318324E-58.4413319
Produced by FastQC (version 0.11.5)