FastQCFastQC Report
Wed 25 May 2016
SRR1295271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4084775
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG61260.1499715406601343No Hit
GTATCAACGCAGAGTACTTTTTTTT54250.13281025270669744No Hit
ATTCCATTCCATTCCATTCCATTCC44100.10796188284544436No Hit
GGTATCAACGCAGAGTACTTTTTTT41710.10211088738057787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC1606.1045284E-910.1016151
GGTATCA35500.09.9626861
GTGGTAT17500.09.8876991
CGTGTCG1356.467526E-79.8499929
GTTCTAG4800.09.5074011
TAACCCG900.00111226339.5019295
TGGTATC17300.09.2820032
TAGACAG6000.09.0268335
GTCCTAA3700.08.9934881
GTCTTAG4400.08.859171
CGTAGTA2106.368282E-98.5967732
GTGTTAA5550.08.56522751
CGTACCC1000.00290148338.5516323
GTCTTAT4600.08.47398951
TCTAGAC3600.08.4460563
TATACTG12650.08.4127755
GTATTAG6000.08.3982061
CGCTCAC1252.7507395E-48.35842210
TCTATAC6400.08.3140863
CTATACT7100.08.2974594
Produced by FastQC (version 0.11.5)