##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295271_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4084775
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.000347877178058	31.0	30.0	33.0	26.0	34.0
2	30.002474554902044	31.0	30.0	34.0	25.0	34.0
3	30.370521264941154	31.0	30.0	34.0	26.0	34.0
4	33.87836025240068	35.0	35.0	37.0	28.0	37.0
5	34.03558213120674	35.0	35.0	37.0	28.0	37.0
6	34.1990655544063	36.0	35.0	37.0	30.0	37.0
7	34.212346334865444	37.0	35.0	37.0	30.0	37.0
8	34.10927970328843	36.0	35.0	37.0	29.0	37.0
9	35.196396374341305	39.0	35.0	39.0	27.0	39.0
10	35.12351402464028	38.0	35.0	39.0	27.0	39.0
11	35.4684696219498	38.0	35.0	39.0	28.0	39.0
12	35.493828913465244	39.0	35.0	39.0	29.0	39.0
13	35.391308211590605	39.0	35.0	39.0	28.0	39.0
14	35.59809193896849	39.0	34.0	41.0	25.0	41.0
15	36.21038857709421	39.0	35.0	41.0	27.0	41.0
16	36.301307171141616	39.0	36.0	41.0	27.0	41.0
17	36.11787381189907	39.0	36.0	41.0	27.0	41.0
18	36.08798991376514	39.0	35.0	41.0	27.0	41.0
19	36.15881633627311	39.0	35.0	41.0	27.0	41.0
20	36.12970922511032	39.0	35.0	41.0	27.0	41.0
21	36.13636418162567	39.0	35.0	41.0	27.0	41.0
22	35.87607640567718	39.0	34.0	41.0	27.0	41.0
23	35.562025815375385	39.0	34.0	41.0	25.0	41.0
24	35.89607334553311	39.0	34.0	41.0	27.0	41.0
25	34.55989595510157	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24153251997951486
1101	2	0.5384250970228486
1101	3	0.39968278253665446
1101	4	0.3501893769973563
1101	5	0.24330661588216174
1101	6	0.2841565252662548
1101	7	0.22009660541995402
1101	8	0.19295566832434474
1101	9	0.1485326159718312
1101	10	0.571028358896875
1101	11	0.3841362154364205
1101	12	0.4450187594930597
1101	13	0.40136702271765046
1101	14	0.8873946423216736
1101	15	0.7093578818118402
1101	16	0.5055316067271747
1101	17	0.7733976642483924
1101	18	0.8337180337406593
1101	19	0.8271045536947597
1101	20	0.8432851062802058
1101	21	0.7774196515954586
1101	22	0.5554176882800448
1101	23	-1.6984027716806196
1101	24	0.00955170358784585
1101	25	0.45662356791993375
1102	1	0.1997222066909181
1102	2	0.2962004979489592
1102	3	0.29043804841068166
1102	4	0.4019898036053675
1102	5	0.2068726778772998
1102	6	0.1487068281029238
1102	7	0.12735794634883035
1102	8	0.13259862194617966
1102	9	0.3782609963860537
1102	10	0.8001591459369877
1102	11	0.5690184138975809
1102	12	0.36599286744062454
1102	13	0.4129022463146441
1102	14	1.0391400119208853
1102	15	0.6862134318947
1102	16	0.474819505640518
1102	17	0.8407231038808618
1102	18	0.8170412969856358
1102	19	0.8054169471395625
1102	20	0.8653303811046058
1102	21	0.8500903950777712
1102	22	0.6792617425103273
1102	23	0.8715652078658707
1102	24	0.8447238500817988
1102	25	0.7337933579322922
1103	1	-0.35398216982915187
1103	2	-1.6065564049943077
1103	3	-0.5232444311593909
1103	4	-0.034003628530875574
1103	5	-0.22495984526074153
1103	6	-0.0735639454529391
1103	7	-0.05563912693389739
1103	8	0.14368460930261762
1103	9	0.7673927572830905
1103	10	0.7361271150834199
1103	11	0.5517123737955032
1103	12	0.3763650362095703
1103	13	0.14846060938024408
1103	14	-0.9903535798933447
1103	15	-0.1983457988665478
1103	16	-0.09105385964469548
1103	17	0.32288270736653857
1103	18	0.5434322509684577
1103	19	0.566210903923313
1103	20	0.592913271685191
1103	21	0.6830666155762657
1103	22	0.30993750095021255
1103	23	1.1128240443476827
1103	24	0.9243863708200877
1103	25	0.5980894222822144
1104	1	-0.11097318285798252
1104	2	0.05897291347871558
1104	3	-0.4090692974741579
1104	4	-0.18736444789943363
1104	5	-0.18873563671164817
1104	6	-0.0535098523434101
1104	7	0.005264006680882005
1104	8	-0.10455217000421158
1104	9	-2.302419236551252
1104	10	-0.590453543598656
1104	11	-0.5423160568036138
1104	12	-0.13454860792280954
1104	13	0.05735322201157089
1104	14	-1.8058038748451395
1104	15	-0.564741268227209
1104	16	-0.18088084006041782
1104	17	0.10602973374519564
1104	18	0.21580970452339443
1104	19	0.2622735121475017
1104	20	0.4773581012954722
1104	21	0.6014595619021748
1104	22	0.6725223301444103
1104	23	1.001674739988232
1104	24	0.7268285377659112
1104	25	0.4104580615524398
1105	1	-0.32945453245989853
1105	2	-0.25486136726305375
1105	3	-1.5806712000308565
1105	4	-0.6732279847687366
1105	5	-0.4920477503387275
1105	6	-0.24846319657799398
1105	7	-0.3031211302417489
1105	8	-0.19165305697891455
1105	9	0.17603009136735892
1105	10	0.3148888235543339
1105	11	-0.2404784364490382
1105	12	-0.1898663590368841
1105	13	-0.34692431049170835
1105	14	0.46419366689448793
1105	15	0.05426314712528324
1105	16	-0.4043290432696125
1105	17	0.1539626731841892
1105	18	0.10517078161488058
1105	19	0.014781398101710863
1105	20	-0.40724252038360476
1105	21	-0.0095941892287712
1105	22	0.1108035087328858
1105	23	0.3145397593752648
1105	24	0.2017870742332164
1105	25	0.1882822999996847
1106	1	-1.7130281894434276
1106	2	-1.1622867013627776
1106	3	-1.1674632140558998
1106	4	-1.5037160732647479
1106	5	-1.0078171839668641
1106	6	-0.8866662422718576
1106	7	-0.7370985935391943
1106	8	-0.8779596408161154
1106	9	-0.3665140871491701
1106	10	-2.173147803027227
1106	11	-1.4430506244565393
1106	12	-1.0776824277909398
1106	13	-1.1531595513138484
1106	14	-0.31179659518425495
1106	15	-1.0246923830155055
1106	16	-1.582747414744361
1106	17	-0.8059183541349313
1106	18	-0.6255031629338816
1106	19	-0.5798522867503593
1106	20	-0.2239904538520463
1106	21	-0.2211955478694989
1106	22	-0.33814832007319495
1106	23	-0.6720035351058016
1106	24	-0.8892319880633792
1106	25	-1.5876684398303098
1107	1	-0.16943439112362313
1107	2	0.0718098457419849
1107	3	-0.07830156983550296
1107	4	0.060425888548010676
1107	5	-0.2336365865669663
1107	6	-0.04016133821262713
1107	7	-0.1698551164173807
1107	8	-0.2510825873299325
1107	9	0.2999073878092702
1107	10	-0.16779567361353998
1107	11	-0.14378133806029325
1107	12	-0.29687355173406615
1107	13	-0.15311539249876205
1107	14	-1.2681732226121412
1107	15	-0.649604841878265
1107	16	-0.35150809152209206
1107	17	-0.13876508523475906
1107	18	0.16172130023942088
1107	19	0.13138957526617645
1107	20	0.13156835772215913
1107	21	0.14083502839628892
1107	22	-0.43814494401699733
1107	23	0.4931493529752302
1107	24	0.3314235340167073
1107	25	-0.025584791961072995
1108	1	-0.2233506935288787
1108	2	0.0242556966809353
1108	3	-0.20896714392740634
1108	4	-0.3658617619219058
1108	5	-0.4616221686018207
1108	6	-0.27120545929739137
1108	7	-0.2303072613913244
1108	8	-0.34327285620889825
1108	9	0.21515886014592667
1108	10	0.2577054738413196
1108	11	-0.05211414772857381
1108	12	-0.030511700020930732
1108	13	-0.22839974262205232
1108	14	0.09782566638008205
1108	15	-0.16381769789718703
1108	16	-0.21430617214304704
1108	17	0.03489548429497091
1108	18	0.14067097058180167
1108	19	0.12756841232758376
1108	20	0.18687216304946475
1108	21	0.22009387058542274
1108	22	0.0853573948805888
1108	23	-0.19727612278494178
1108	24	-0.019291960017284282
1108	25	-0.9050032472580938
1109	1	-0.8747268355576132
1109	2	-0.7544142433831915
1109	3	-0.784861182000629
1109	4	-0.733786287553805
1109	5	-1.018730114680288
1109	6	-1.3198552889765693
1109	7	-0.8966859326662941
1109	8	-1.3442796962114798
1109	9	-1.0185448601196256
1109	10	-1.1813280408883031
1109	11	-1.1863724059392382
1109	12	-1.2557372567098142
1109	13	-1.2847591035123784
1109	14	-0.7399394846633669
1109	15	-1.3439984970220138
1109	16	-1.4728018121413982
1109	17	-1.4684052244977437
1109	18	-2.019725788058487
1109	19	-2.2864055646437293
1109	20	-1.940350762317678
1109	21	-1.6858285703287024
1109	22	-1.884448466366905
1109	23	-1.2709707260659187
1109	24	-2.226462203289074
1109	25	-1.2286181335278599
1110	1	0.0703783101854043
1110	2	0.21707193107502576
1110	3	0.001419602412390475
1110	4	0.2594510642196681
1110	5	0.1905206349350621
1110	6	0.13562913533366583
1110	7	0.026211537403263208
1110	8	-0.01872237473056515
1110	9	0.2848079934370702
1110	10	0.16899966277383527
1110	11	0.18290397145403148
1110	12	0.2519316876013775
1110	13	0.19105788883350527
1110	14	-2.409789592956983
1110	15	-0.6442553022976654
1110	16	-0.13938158444538118
1110	17	-0.19078650355995563
1110	18	-2.846390709709567
1110	19	-0.5938720358230256
1110	20	-2.0296469450675687
1110	21	-0.4330789589653463
1110	22	-1.8112335859460913
1110	23	-0.5756177995151077
1110	24	0.020318285725885232
1110	25	-0.06007571767354136
1111	1	-0.2624066355773742
1111	2	-1.3030247111566062
1111	3	-0.36756880508914236
1111	4	-0.04514564981571567
1111	5	-0.09755493742788701
1111	6	-0.008955655833460696
1111	7	-0.11340517288962104
1111	8	-0.08122894767519995
1111	9	-1.8485716950216755
1111	10	-0.31613829744108557
1111	11	-0.2985752611234034
1111	12	-0.1670971593753947
1111	13	-0.08179197858340359
1111	14	0.875778310366151
1111	15	0.17573357084033603
1111	16	0.21553002350177053
1111	17	-0.22607818455110618
1111	18	-0.012544981343893369
1111	19	-0.1725733924062709
1111	20	0.129956140644353
1111	21	-0.5356268453601984
1111	22	-0.19356978544593062
1111	23	0.027031015577890116
1111	24	-0.5336735814110654
1111	25	-1.8299210467668274
1112	1	0.3102128838378917
1112	2	0.4284051234060904
1112	3	0.16572654327974945
1112	4	0.3739617230009955
1112	5	0.18002432801839774
1112	6	0.15695043719842516
1112	7	0.11015094594927888
1112	8	0.1602383098161937
1112	9	-0.2760462138184536
1112	10	0.6003227982119981
1112	11	0.2227120035026502
1112	12	0.19187276533144626
1112	13	0.19952226283769647
1112	14	0.5476350411368003
1112	15	0.2512123857131243
1112	16	0.2896238743276669
1112	17	-3.5509483855881
1112	18	-0.9844938221635928
1112	19	-1.111898106467521
1112	20	-0.1295979070069535
1112	21	-0.530862787165205
1112	22	-1.598951766960603
1112	23	-1.4848117707547743
1112	24	-0.27193353073212734
1112	25	0.23397746964290178
1113	1	-0.23724706223396907
1113	2	-0.02953186766880833
1113	3	-0.01670198343944662
1113	4	-0.4199512982509148
1113	5	-0.1340093670213136
1113	6	-0.20495362673932505
1113	7	-0.3034378494711234
1113	8	-0.2355426319210281
1113	9	0.15662398533365973
1113	10	0.41171514808178244
1113	11	-0.09732897772683913
1113	12	-0.04221655002012881
1113	13	-0.3077582772473946
1113	14	-1.491239695930382
1113	15	-1.7739959956786677
1113	16	-0.5707346114216847
1113	17	-0.3740760125527558
1113	18	-0.7992489009296477
1113	19	-0.27844263650126777
1113	20	0.12787906240866675
1113	21	-1.7861491110774779
1113	22	-2.178115395588634
1113	23	-0.7822580140441104
1113	24	-2.4752412935873735
1113	25	-0.708312060059832
1114	1	0.23849056107233224
1114	2	0.26334473931354907
1114	3	0.05147861318903324
1114	4	0.2557094403641429
1114	5	0.04203352143122174
1114	6	0.22559841607527886
1114	7	0.18264037374494535
1114	8	0.0979885517148773
1114	9	0.48949172702183574
1114	10	0.36327315441644714
1114	11	0.2796652928448893
1114	12	0.23687528844344286
1114	13	0.20504833501794195
1114	14	1.0563293190843197
1114	15	0.45167144101719714
1114	16	0.39915258162045575
1114	17	-0.3836878267122472
1114	18	-0.0597478129121356
1114	19	-0.43696179042043326
1114	20	-1.692986291434167
1114	21	-0.7921009447373493
1114	22	-1.4729472692132646
1114	23	-2.9890320882788544
1114	24	-1.9361667455395803
1114	25	-1.338101358354706
1115	1	0.39430289607358304
1115	2	0.4280036715284936
1115	3	0.34354727848168665
1115	4	0.5935270335682716
1115	5	0.5186261937997187
1115	6	0.3785819640475765
1115	7	0.4291143465810663
1115	8	0.5373996931014986
1115	9	0.6610428083663322
1115	10	0.9534305077692338
1115	11	0.6668135244565718
1115	12	0.4911657629428774
1115	13	0.4719663879598883
1115	14	1.1249813823361592
1115	15	0.7524443146053343
1115	16	0.6106246940432598
1115	17	0.577341676972253
1115	18	0.8247584566254318
1115	19	0.22103625365642898
1115	20	0.743043239907621
1115	21	-0.30417533140019515
1115	22	0.7564000225670284
1115	23	0.793764841246329
1115	24	0.8310262208074022
1115	25	1.0359757180712208
1116	1	0.8020715248232833
1116	2	0.8038662204044869
1116	3	0.6676788375661431
1116	4	0.8320152073306062
1116	5	0.7513494894002974
1116	6	0.6429432263256203
1116	7	0.5432122702466486
1116	8	0.6233254645777322
1116	9	0.7864046100707611
1116	10	0.8042756852872373
1116	11	0.6374346470120145
1116	12	0.6735763994964756
1116	13	0.16428611440671403
1116	14	1.2562284680622895
1116	15	0.9970512698532659
1116	16	0.9339699198150413
1116	17	1.084336086645763
1116	18	0.9989726820406446
1116	19	1.1893616141121086
1116	20	1.0188939539110748
1116	21	0.46813744142409064
1116	22	0.5128509423855121
1116	23	-0.2768963065432928
1116	24	0.20505268825540668
1116	25	-0.17936265769664317
1201	1	0.32347409083388
1201	2	0.5124159394689549
1201	3	0.4545895993491804
1201	4	0.36176087414159497
1201	5	0.3361426412757922
1201	6	0.17533870354338177
1201	7	0.17578048212596542
1201	8	0.25187458946262353
1201	9	0.43007419654098555
1201	10	0.7676921512582311
1201	11	0.4269110096136828
1201	12	0.48323166393392114
1201	13	0.4456640783095338
1201	14	0.9754923205459392
1201	15	0.6153291043811677
1201	16	0.3619203319438853
1201	17	0.5681287246327642
1201	18	0.44264278073463714
1201	19	0.6946858344723097
1201	20	0.7563874406656694
1201	21	0.7366984396560241
1201	22	0.6819083238694148
1201	23	0.29661181290780547
1201	24	0.5990578517271459
1201	25	0.8305502296869847
1202	1	0.23265726816553922
1202	2	0.6444704236087091
1202	3	0.431157588125231
1202	4	0.4158084064074359
1202	5	0.2421901894691203
1202	6	0.20849551197273541
1202	7	0.25025243261309527
1202	8	0.22437869896750584
1202	9	0.827208372390956
1202	10	-7.620107860262237E-4
1202	11	0.34984235735826275
1202	12	0.33081680334233
1202	13	0.3417420626974703
1202	14	1.0130332407953588
1202	15	0.6760379524365945
1202	16	0.5949547736643837
1202	17	-1.3733315282056608
1202	18	0.15397132890108622
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2210	19	0.7056137882752296
2210	20	0.8598351180245913
2210	21	0.8150382195587937
2210	22	0.9982884571560646
2210	23	1.2919185354102112
2210	24	0.9920937382058312
2210	25	0.9321580623436674
2211	1	0.15718889357463794
2211	2	-0.5466417642929606
2211	3	0.020872558225537574
2211	4	0.3063768463324834
2211	5	0.31587548328408843
2211	6	0.28371980361762894
2211	7	0.2930734238402195
2211	8	0.3233336338759827
2211	9	0.8600655892593139
2211	10	0.8749632932217182
2211	11	0.47698608287700495
2211	12	0.3636817327816857
2211	13	0.32611484665194723
2211	14	-0.8587486420706156
2211	15	0.11978478037804052
2211	16	0.2881943652685095
2211	17	0.33824750232182055
2211	18	0.3894916097980783
2211	19	0.6560940151321901
2211	20	0.7197327318600486
2211	21	0.7617359413129492
2211	22	0.11892586055004273
2211	23	0.6076014684311701
2211	24	0.018404614658265928
2211	25	0.5315094117259207
2212	1	0.10134305983905989
2212	2	-1.0633722081727797
2212	3	-0.28190508832340555
2212	4	0.16553128988913812
2212	5	0.21503388424591208
2212	6	0.2552117659086264
2212	7	0.13939622075344715
2212	8	0.23115532880934353
2212	9	-2.189426692088915
2212	10	0.004591523077124293
2212	11	0.10486778247982897
2212	12	-0.017006951572803075
2212	13	0.09399660102854313
2212	14	0.7011748252736041
2212	15	0.36136225137704514
2212	16	0.43014484566874245
2212	17	0.48283486067170855
2212	18	0.11104385747412948
2212	19	-0.4463945628242385
2212	20	0.4714244541049766
2212	21	0.38012082703774297
2212	22	-1.6697301485095437
2212	23	-0.36685663065869534
2212	24	0.31926202962368677
2212	25	-0.16452565170553157
2213	1	0.25227264418978024
2213	2	0.5359434216011465
2213	3	0.19363493096365758
2213	4	0.3145047231526732
2213	5	0.29544977925456095
2213	6	0.20139151106464936
2213	7	0.1735515223181494
2213	8	0.20953301632309973
2213	9	0.6361333705892491
2213	10	0.6415786756577759
2213	11	0.011123955573033584
2213	12	0.04747719427123087
2213	13	0.05907026829803641
2213	14	-1.6507571930509783
2213	15	-0.34229588062996896
2213	16	-0.08880779562531416
2213	17	-2.0244809200943976
2213	18	-0.8413116592635461
2213	19	-0.5406862150675522
2213	20	0.17572592129579334
2213	21	-0.1917152063977241
2213	22	0.3726848172954291
2213	23	0.9151578940412222
2213	24	0.7376220585292117
2213	25	-0.6681914974434022
2214	1	0.346129595780468
2214	2	0.27301629535109484
2214	3	0.19505162807783094
2214	4	0.42456538576831804
2214	5	0.42557629924056783
2214	6	0.3927201760401857
2214	7	0.3289554767214682
2214	8	0.3474253806203862
2214	9	0.6768397490540607
2214	10	0.6637541314891351
2214	11	0.009375830999573509
2214	12	-0.02567882250654918
2214	13	0.13539216102407892
2214	14	0.8972858768881693
2214	15	0.5324013689461182
2214	16	0.46500414270300894
2214	17	0.5075875571594963
2214	18	0.42693425775688354
2214	19	-0.7989203000749683
2214	20	0.22526654555652215
2214	21	0.5069306233115896
2214	22	0.6577797903652538
2214	23	1.2311611534484257
2214	24	0.9752348512395201
2214	25	1.0146476878844766
2215	1	0.010775382496277075
2215	2	0.3952120412479303
2215	3	0.3161591030024766
2215	4	0.4338068766454981
2215	5	0.43588585135044156
2215	6	0.29949579670243764
2215	7	0.232521790751683
2215	8	0.2512799419350884
2215	9	0.29792898821376923
2215	10	0.6765620773947134
2215	11	0.4079249597013046
2215	12	0.27947024312199886
2215	13	0.12813390358302001
2215	14	1.029381575174618
2215	15	0.5086486741183549
2215	16	0.44148291989156974
2215	17	0.6074857564717036
2215	18	0.5438330309316939
2215	19	-0.25067758392358286
2215	20	-1.580331035290662
2215	21	-1.7910434208029855
2215	22	-0.08583860839358692
2215	23	0.6285671628849627
2215	24	0.7175474188305273
2215	25	0.034179602118101116
2216	1	0.30969627511253606
2216	2	-0.027239497667380874
2216	3	0.19939243399818452
2216	4	0.382581633154075
2216	5	0.5818458634786055
2216	6	0.3515271092253869
2216	7	0.2818078173758849
2216	8	0.3346477037115747
2216	9	0.6313887425153482
2216	10	-1.3117752402187648
2216	11	-0.1063146478247532
2216	12	0.21392412516530612
2216	13	0.3040361934956053
2216	14	-0.11208616968844609
2216	15	-0.09910442908240924
2216	16	0.30415324013281264
2216	17	0.6255086597227972
2216	18	0.5207567782038893
2216	19	-0.9713150549954364
2216	20	-0.5473800244041911
2216	21	-0.508319812963407
2216	22	-0.3395070681581984
2216	23	-3.9125388512810595
2216	24	-1.5482966435263847
2216	25	-0.6114917473668626
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	28.0
5	53.0
6	71.0
7	249.0
8	133.0
9	416.0
10	1237.0
11	2908.0
12	5690.0
13	9501.0
14	14062.0
15	17915.0
16	21721.0
17	23637.0
18	24910.0
19	26060.0
20	27075.0
21	27970.0
22	29565.0
23	31633.0
24	34269.0
25	38353.0
26	43013.0
27	49658.0
28	57898.0
29	71343.0
30	87219.0
31	108629.0
32	141610.0
33	189347.0
34	254436.0
35	346923.0
36	488979.0
37	725905.0
38	1182343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.730801647239154	21.378950267254947	14.185901443980345	22.704346641525557
2	15.406117403471503	20.920223121492302	41.15262461089511	22.521034864141086
3	17.726787079073077	26.651070862628522	30.26280635824915	25.359335700049247
4	12.337713067225172	17.52102539210541	39.03761611078553	31.10364542988389
5	13.282574895882012	37.7107954506489	35.34197039923897	13.664659254230116
6	30.296644250933785	38.90205757084951	17.91769961448857	12.883598563728139
7	25.683785713516166	33.31064331586365	23.922411893356266	17.083159077263918
8	23.304932723345928	37.67913849442237	21.082744786588897	17.933183995642807
9	24.104246971871344	17.66012470061567	22.320332665585862	35.915295661927125
10	15.090843646086869	30.16893000386881	33.483155971929065	21.25707037811525
11	31.80930338816338	26.071774087507865	23.852159904876476	18.26676261945228
12	20.86439724723672	27.6813475974533	31.608444961016723	19.84581019429326
13	25.85753639335431	24.544804534936397	27.335431027094533	22.262228044614762
14	20.82356173884422	23.256786727480762	28.28896196768076	27.630689565994267
15	22.46755371306599	31.114933667436944	24.27165444961556	22.1458581698815
16	21.950836688174707	31.50780663282809	25.710626046334646	20.83073063266256
17	20.181331659115877	30.538201492789486	28.570375655807272	20.71009119228737
18	20.2779861249363	30.377725848115777	29.254316336857812	20.08997169009011
19	21.03510045140205	28.824013923045204	29.64070492761799	20.500180697934756
20	22.214912302179197	28.51905318281046	28.78372833018139	20.482306184828946
21	22.678091248942014	28.99964229831048	27.858326626936723	20.463939825810783
22	21.538876283002917	28.4940198150326	29.489031899788227	20.47807200217626
23	21.828227011758806	28.83752749695893	28.761941466491418	20.572304024790846
24	21.287676599495605	27.96432506549791	28.9765431796479	21.771455155358584
25	22.091377813678978	28.251388244197656	28.50526452001233	21.151969422111033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2995.0
1	2995.0
2	3439.5
3	3884.0
4	3884.0
5	3884.0
6	5619.5
7	7355.0
8	7355.0
9	7355.0
10	12034.5
11	16714.0
12	16714.0
13	16714.0
14	28194.0
15	39674.0
16	39674.0
17	39674.0
18	61508.0
19	83342.0
20	83342.0
21	83342.0
22	117615.0
23	151888.0
24	151888.0
25	151888.0
26	196427.0
27	240966.0
28	240966.0
29	240966.0
30	291069.5
31	341173.0
32	341173.0
33	341173.0
34	386157.5
35	431142.0
36	431142.0
37	431142.0
38	468594.0
39	506046.0
40	506046.0
41	506046.0
42	509864.5
43	513683.0
44	513683.0
45	513683.0
46	493183.5
47	472684.0
48	472684.0
49	472684.0
50	446764.5
51	420845.0
52	420845.0
53	420845.0
54	378070.5
55	335296.0
56	335296.0
57	335296.0
58	285691.5
59	236087.0
60	236087.0
61	236087.0
62	192211.5
63	148336.0
64	148336.0
65	148336.0
66	113340.0
67	78344.0
68	78344.0
69	78344.0
70	56430.0
71	34516.0
72	34516.0
73	34516.0
74	23864.0
75	13212.0
76	13212.0
77	13212.0
78	8924.5
79	4637.0
80	4637.0
81	4637.0
82	3049.0
83	1461.0
84	1461.0
85	1461.0
86	914.0
87	367.0
88	367.0
89	367.0
90	224.0
91	81.0
92	81.0
93	81.0
94	52.0
95	23.0
96	23.0
97	23.0
98	23.5
99	24.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08027369928576238
2	0.017675392157462774
3	0.02932842078205042
4	0.03667276655384935
5	0.04668555795606857
6	0.014443880017871242
7	0.01828742097177935
8	0.03456738743260033
9	0.03415120783886505
10	0.020441762398173705
11	0.020123507414729087
12	0.014394917712725917
13	0.022645066129713388
14	0.03699102153729397
15	0.034542906280027666
16	0.021445489653652894
17	0.021469970806225556
18	0.025607285591005623
19	0.014957984221897168
20	0.012803642795502812
21	0.008960101841594703
22	0.017234731411154836
23	0.016818551817419564
24	0.016524777986547605
25	0.10872079857519691
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4084775.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.1480756652291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.61095363230487	59.48758455473189
2	11.070544887119665	15.7529593056821
3	2.6711853236708047	5.701490865731397
4	0.9537045550568733	2.7141697538184033
5	0.4641561859260652	1.651190971837592
6	0.27759773978790026	1.1850326996955662
7	0.17918723411191453	0.8924178823586331
8	0.12553012944785405	0.7144981718577539
9	0.09135689981691025	0.5849880858642857
>10	0.5074344784095873	6.395221348448979
>50	0.031054998941313403	1.544558587517525
>100	0.016049141037330933	1.976737434685077
>500	6.915524824095381E-4	0.34646029346815876
>1k	4.8408665071053423E-4	0.7685203153534178
>5k	6.915523581578542E-5	0.2841697289492945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6126	0.1499715406601343	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5425	0.13281025270669744	No Hit
ATTCCATTCCATTCCATTCCATTCC	4410	0.10796188284544436	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4171	0.10211088738057787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.896230514532624E-5	0.0
10	0.0	0.0	0.0	4.896230514532624E-5	0.0
11	0.0	0.0	0.0	7.344345771798936E-5	0.0
12	0.0	0.0	0.0	7.344345771798936E-5	0.0
13	0.0	0.0	0.0	7.344345771798936E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAC	160	6.1045284E-9	10.101615	1
GGTATCA	3550	0.0	9.962686	1
GTGGTAT	1750	0.0	9.887699	1
CGTGTCG	135	6.467526E-7	9.849992	9
GTTCTAG	480	0.0	9.507401	1
TAACCCG	90	0.0011122633	9.501929	5
TGGTATC	1730	0.0	9.282003	2
TAGACAG	600	0.0	9.026833	5
GTCCTAA	370	0.0	8.993488	1
GTCTTAG	440	0.0	8.85917	1
CGTAGTA	210	6.368282E-9	8.596773	2
GTGTTAA	555	0.0	8.5652275	1
CGTACCC	100	0.0029014833	8.551632	3
GTCTTAT	460	0.0	8.4739895	1
TCTAGAC	360	0.0	8.446056	3
TATACTG	1265	0.0	8.412775	5
GTATTAG	600	0.0	8.398206	1
CGCTCAC	125	2.7507395E-4	8.358422	10
TCTATAC	640	0.0	8.314086	3
CTATACT	710	0.0	8.297459	4
>>END_MODULE