FastQCFastQC Report
Wed 25 May 2016
SRR1295271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4084775
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT158180.3872428713943853No Hit
GGTATCAACGCAGAGTACTTTTTTT126430.30951521197617987No Hit
TATCAACGCAGAGTACTTTTTTTTT94720.2318854771682651No Hit
GTGGTATCAACGCAGAGTACATGGG69090.1691402831245295No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56900.13929775813845316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGT500.001504521813.29501213
TTACTCG905.4078737E-712.667500519
CCCGACG550.003078551512.08637512
AGTGCCG658.053624E-411.68792410
GGTATCA48000.011.4224311
AACCGTC752.0828687E-411.3955877
ACACCGT1354.7657522E-911.2550376
AGCGTCA3000.011.0913973
CGGCGTA600.005894864411.07917812
ATACCGA600.005894864411.0791786
GGACCGT951.3672574E-510.9958766
GCCGCTA700.001496978510.85307214
CTAGAAC4300.010.3912753
GAACCGC750.002657403210.1295356
CGATCAA750.002657403210.12953510
CACCGTC1252.5855916E-69.8761747
GTGGTAT20400.09.5653521
TGGTATC20300.09.5537432
GTCGTAG800.00444543139.5186931
GTCTACG1106.696449E-59.5186921
Produced by FastQC (version 0.11.5)