FastQCFastQC Report
Wed 25 May 2016
SRR1295270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4003245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG57890.14460768701390997No Hit
GTATCAACGCAGAGTACTTTTTTTT47210.11792932983117445No Hit
ATTCCATTCCATTCCATTCCATTCC41460.10356598209702379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG508.732381E-515.19735059
TAGACCG856.5871375E-410.060595
AGCACCG1856.475602E-109.7584715
TATACCG1106.82879E-59.5016695
AACGATT1151.1140183E-49.085031517
GGTATCA37500.09.0518111
AGTACCG950.00182185129.001585
CTAAGAC3950.08.9002973
GGACCGG1201.7683556E-48.7068166
TATACTG8850.08.6964435
GTATTAG5600.08.6592581
GTGGTAT19900.08.6003871
TCTAGAC4100.08.57467653
CCGTGCA2002.5493136E-88.548519
TCTATAC6450.08.5441363
CTAGACT3250.08.4784124
GTATTAA6300.08.4517381
TGGACTG7100.08.4310585
CTAGGAC4000.08.313963
GTGTTAG4850.08.233911
Produced by FastQC (version 0.11.5)