##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4003245 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.046547738147428 31.0 30.0 33.0 26.0 34.0 2 29.92470708138023 31.0 30.0 34.0 25.0 34.0 3 30.38546703986391 31.0 30.0 34.0 26.0 34.0 4 33.80877937772982 35.0 35.0 37.0 28.0 37.0 5 34.12641219810429 35.0 35.0 37.0 30.0 37.0 6 34.20329407767948 37.0 35.0 37.0 30.0 37.0 7 34.180885256835396 37.0 35.0 37.0 30.0 37.0 8 34.110566802681326 37.0 35.0 37.0 29.0 37.0 9 35.18092072805936 39.0 35.0 39.0 27.0 39.0 10 35.1078597487788 38.0 35.0 39.0 27.0 39.0 11 35.47148275961126 39.0 35.0 39.0 28.0 39.0 12 35.485799645037964 39.0 35.0 39.0 28.0 39.0 13 35.392484347073434 39.0 35.0 39.0 28.0 39.0 14 35.601171299783054 39.0 34.0 41.0 25.0 41.0 15 36.20906014995335 39.0 36.0 41.0 27.0 41.0 16 36.30192231552153 39.0 36.0 41.0 27.0 41.0 17 36.124356365898166 39.0 36.0 41.0 27.0 41.0 18 36.09929444737956 39.0 35.0 41.0 27.0 41.0 19 36.17236017280981 39.0 35.0 41.0 27.0 41.0 20 36.13456783184642 39.0 35.0 41.0 27.0 41.0 21 36.137426013146836 39.0 35.0 41.0 27.0 41.0 22 35.87941857168372 39.0 34.0 41.0 27.0 41.0 23 35.5673592298248 39.0 34.0 41.0 25.0 41.0 24 35.886081166653554 39.0 34.0 41.0 27.0 41.0 25 34.57239964079141 38.0 32.0 40.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13155107770834817 1101 2 0.36412869554702354 1101 3 0.213969638043924 1101 4 0.2654436011869379 1101 5 0.1414920421795287 1101 6 0.1453225905677158 1101 7 0.1053942785276547 1101 8 0.12077021612437022 1101 9 0.10273648895201148 1101 10 0.42420692202651367 1101 11 0.28480987418149795 1101 12 0.29079833843446323 1101 13 0.32955854318111477 1101 14 0.7578568807733248 1101 15 0.5285188927445574 1101 16 0.41634599672588024 1101 17 0.6336485575046211 1101 18 0.6354578985951989 1101 19 0.7010799782443939 1101 20 0.7906355124681923 1101 21 0.7538317302882405 1101 22 0.6708904116334224 1101 23 -1.5995071466930426 1101 24 0.03923590908540575 1101 25 0.44963477339289426 1102 1 0.2555686400052295 1102 2 0.2834061072765799 1102 3 0.23214583947385137 1102 4 0.45680083064818433 1102 5 0.1665726594515533 1102 6 0.2279672592041635 1102 7 0.2387686205811832 1102 8 0.24809656193965424 1102 9 0.45513260441926207 1102 10 0.9084439741244523 1102 11 0.5815056537380556 1102 12 0.4602168930291768 1102 13 0.4886727548250178 1102 14 1.0174087349322605 1102 15 0.7526054199650645 1102 16 0.5550888023661287 1102 17 0.8228123981893987 1102 18 0.8330564713313819 1102 19 0.8287114015830213 1102 20 0.9130162396431203 1102 21 0.8246410087293157 1102 22 0.7481776529574802 1102 23 0.8084553837407213 1102 24 0.8329434640872577 1102 25 0.7677039253200633 1103 1 -0.39253532587786566 1103 2 -1.5394618082491043 1103 3 -0.4334980164005877 1103 4 -0.10852680961878036 1103 5 -0.0983571181602585 1103 6 -0.016470294881443692 1103 7 0.04384711139996256 1103 8 0.1843191783200382 1103 9 0.7129024101259631 1103 10 0.7022977740462153 1103 11 0.4783521661792278 1103 12 0.3024563485574703 1103 13 0.045804276216244943 1103 14 -1.0724161314095966 1103 15 -0.15621579522751006 1103 16 -0.10542410705454586 1103 17 0.5325505497358023 1103 18 0.5766474744490822 1103 19 0.579348130011013 1103 20 0.5983554082953972 1103 21 0.5917498437993132 1103 22 0.24720900186746775 1103 23 0.9760513597711622 1103 24 0.8230975858554572 1103 25 0.7146482364027236 1104 1 -0.06897660115651405 1104 2 -0.01314865550556732 1104 3 -0.36728971558828505 1104 4 -0.22638186183826292 1104 5 -0.17961305453879817 1104 6 -0.04643380422066201 1104 7 -0.0630150066586026 1104 8 -0.046175611088223434 1104 9 -2.088277690307237 1104 10 -0.5243185727787036 1104 11 -0.5615757868335649 1104 12 -0.09403439998372676 1104 13 0.0954258707997937 1104 14 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0.22648108345036633 2213 7 0.1833247895189416 2213 8 0.21383891582890868 2213 9 0.6619413784040589 2213 10 0.6788382625432234 2213 11 0.06447776159829743 2213 12 0.13434180006248653 2213 13 0.16565871525546783 2213 14 -1.5663956844652347 2213 15 -0.2561297761740988 2213 16 0.0029809299374434772 2213 17 -2.0228846129276192 2213 18 -0.7781953095784786 2213 19 -0.4126025642222899 2213 20 0.2310032495845391 2213 21 -0.2025625105130402 2213 22 0.33902084944018185 2213 23 0.8086401658925766 2213 24 0.5969996077466106 2213 25 -0.8283454546887938 2214 1 0.3422809463735277 2214 2 0.26814838000554886 2214 3 0.2200534854424383 2214 4 0.49955099060867525 2214 5 0.4365855847538995 2214 6 0.4165788719263688 2214 7 0.2725800926632189 2214 8 0.30295444299769514 2214 9 0.5769203846543149 2214 10 0.7103302548295574 2214 11 0.05389171457777309 2214 12 0.005024131120926256 2214 13 0.07492249795990347 2214 14 0.935028608106947 2214 15 0.5825222472951381 2214 16 0.49015737317225927 2214 17 0.5727314544712101 2214 18 0.4614745346437914 2214 19 -0.9018083576766145 2214 20 0.2175155517851266 2214 21 0.5203881028874022 2214 22 0.7434504622525253 2214 23 1.2524102877520633 2214 24 0.9901918886115268 2214 25 1.1007531133596729 2215 1 0.03105821935864128 2215 2 0.4674326172386216 2215 3 0.34227977580078317 2215 4 0.45211795585361614 2215 5 0.36841880721619447 2215 6 0.3858856443416556 2215 7 0.3945012728123203 2215 8 0.29957846299229374 2215 9 0.5009049692832974 2215 10 0.8089324203385218 2215 11 0.5447117648736821 2215 12 0.3720553830010118 2215 13 0.1929606135304951 2215 14 1.0477070975793552 2215 15 0.6066620513714085 2215 16 0.5506749088443712 2215 17 0.7290587526386574 2215 18 0.6322468674219124 2215 19 -0.2094419572723254 2215 20 -1.4337847333854867 2215 21 -1.7750256179616812 2215 22 -0.0036802125825019516 2215 23 0.6786054173336566 2215 24 0.7164272411728163 2215 25 0.12814083709999835 2216 1 0.20966513139644505 2216 2 -0.008479019156357737 2216 3 0.08266964617024897 2216 4 0.3691442474423212 2216 5 0.4620953168955637 2216 6 0.23743512227616037 2216 7 0.2551965615486438 2216 8 0.29497991164215165 2216 9 0.5572012094302323 2216 10 -1.6497403227949476 2216 11 -0.21150609281196608 2216 12 0.05322326490151852 2216 13 0.23842441592336883 2216 14 -0.19659451047036214 2216 15 -0.16153511576838042 2216 16 0.2611731268566899 2216 17 0.5890930089177573 2216 18 0.4346389040625027 2216 19 -0.9445697008816509 2216 20 -0.672248235872253 2216 21 -0.5348842806466223 2216 22 -0.3951611097860166 2216 23 -4.2597103666497524 2216 24 -1.9014826615776315 2216 25 -0.8755017804294596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 44.0 5 64.0 6 90.0 7 370.0 8 177.0 9 440.0 10 1300.0 11 3059.0 12 5940.0 13 9797.0 14 14288.0 15 18095.0 16 21576.0 17 23977.0 18 25244.0 19 25866.0 20 26849.0 21 28112.0 22 28944.0 23 31056.0 24 33661.0 25 36944.0 26 41709.0 27 47737.0 28 56399.0 29 69343.0 30 84104.0 31 104661.0 32 136685.0 33 182526.0 34 245700.0 35 336456.0 36 475514.0 37 706945.0 38 1179554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99880797139131 21.08788110914662 13.784980839540149 23.12833007992192 2 15.858431533725009 21.18017861582108 40.22751093394163 22.733878916512282 3 17.984468540523576 26.383265924393704 30.286196051816844 25.346069483265875 4 12.796261352473385 17.442531988591735 37.97900421849827 31.782202440436606 5 13.478328239142876 37.60419764625908 35.2549302849831 13.662543829614949 6 30.819670902395025 38.32483022940891 17.521849062826362 13.3336498053697 7 25.84903901962837 32.89952649951191 23.943968484271245 17.307465996588476 8 23.528121132881925 37.00278482480393 21.554154146949987 17.914939895364157 9 24.363199820475714 17.289558128859152 22.1658515521079 36.18139049855723 10 15.242273621887392 29.74001250802365 33.56470428618438 21.453009583904578 11 32.0401252389582 25.7464203430604 23.81644012433947 18.39701429364193 12 21.01351003596247 27.285830427237336 31.502463706487887 20.19819583031231 13 26.047445308365518 24.29991659516372 27.261712782528264 22.3909253139425 14 21.06125315187213 23.1173608490625 28.05302392299061 27.76836207607476 15 22.74411885462509 30.831473095754358 24.207611260032852 22.2167967895877 16 22.24887962292965 31.05750702609349 25.600416168610252 21.093197182366605 17 20.409434999888813 30.269271236882684 28.394274516290473 20.927019246938034 18 20.475823642309194 30.048467406000544 29.102482189846206 20.373226761844055 19 21.284549359869 28.651589946388 29.3201284609323 20.743732232810704 20 22.41374098096969 28.31776268335686 28.530054523300258 20.73844181237319 21 22.836049248732483 28.761071895934297 27.681376230356392 20.72150262497683 22 21.833603216511104 28.259704488539107 29.139088070530583 20.76760422441921 23 22.038823744576998 28.617707992912244 28.462475976889845 20.880992285620913 24 21.655514488087892 27.752371914849178 28.704610808081338 21.887502788981593 25 22.384763966314196 28.069818317130284 28.12741007633221 21.418007640223305 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3261.0 1 3261.0 2 3471.0 3 3681.0 4 3681.0 5 3681.0 6 5300.0 7 6919.0 8 6919.0 9 6919.0 10 11020.0 11 15121.0 12 15121.0 13 15121.0 14 25008.5 15 34896.0 16 34896.0 17 34896.0 18 54846.0 19 74796.0 20 74796.0 21 74796.0 22 106297.0 23 137798.0 24 137798.0 25 137798.0 26 180699.0 27 223600.0 28 223600.0 29 223600.0 30 273652.5 31 323705.0 32 323705.0 33 323705.0 34 368904.0 35 414103.0 36 414103.0 37 414103.0 38 451962.0 39 489821.0 40 489821.0 41 489821.0 42 497844.0 43 505867.0 44 505867.0 45 505867.0 46 486763.0 47 467659.0 48 467659.0 49 467659.0 50 443938.5 51 420218.0 52 420218.0 53 420218.0 54 380053.0 55 339888.0 56 339888.0 57 339888.0 58 291019.0 59 242150.0 60 242150.0 61 242150.0 62 198395.0 63 154640.0 64 154640.0 65 154640.0 66 118829.0 67 83018.0 68 83018.0 69 83018.0 70 60762.5 71 38507.0 72 38507.0 73 38507.0 74 27014.5 75 15522.0 76 15522.0 77 15522.0 78 10529.0 79 5536.0 80 5536.0 81 5536.0 82 3716.0 83 1896.0 84 1896.0 85 1896.0 86 1184.0 87 472.0 88 472.0 89 472.0 90 295.5 91 119.0 92 119.0 93 119.0 94 75.0 95 31.0 96 31.0 97 31.0 98 26.0 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08345729526921285 2 0.019983788151861803 3 0.03254859495234491 4 0.03956790054068637 5 0.048835382296112284 6 0.019609092124014394 7 0.023131234785780035 8 0.03889344769056104 9 0.038918427425750864 10 0.025329451482484833 11 0.024380221545271398 12 0.01846002430528234 13 0.026953134269823605 14 0.0413414617391641 15 0.037869278547778114 16 0.025504309628813625 17 0.0249297757194476 18 0.029326209112857195 19 0.01955913265363474 20 0.01838508509971286 21 0.013563996208076199 22 0.022381842730085217 23 0.021207795176163335 24 0.0221820048485666 25 0.11013565245194835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4003245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.97870500594509 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22861780581651 59.906881290974155 2 11.106014366097646 15.987930636982615 3 2.8458521680418327 6.14522261082037 4 1.0961105088307235 3.1558645987617204 5 0.4980751712042808 1.792540290944926 6 0.3086928146082951 1.3331585424087224 7 0.19747147051845576 0.9949618506476677 8 0.14465911036707157 0.8329900345227119 9 0.0949215247564646 0.6149095586244072 >10 0.445561082789079 5.486926739110631 >50 0.022205859417950388 1.0885932523122448 >100 0.010493427836914399 1.2501536502917736 >500 8.0178595920886E-4 0.3958256942099371 >1k 4.8804350523415764E-4 0.8687839115832864 >5k 3.486025037386831E-5 0.1452573378048648 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 5789 0.14460768701390997 No Hit GTATCAACGCAGAGTACTTTTTTTT 4721 0.11792932983117445 No Hit ATTCCATTCCATTCCATTCCATTCC 4146 0.10356598209702379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.4979735189827253E-5 0.0 5 0.0 0.0 0.0 2.4979735189827253E-5 0.0 6 0.0 0.0 0.0 2.4979735189827253E-5 0.0 7 0.0 0.0 0.0 2.4979735189827253E-5 0.0 8 0.0 0.0 0.0 2.4979735189827253E-5 0.0 9 0.0 0.0 0.0 7.493920556948176E-5 0.0 10 0.0 0.0 0.0 7.493920556948176E-5 0.0 11 0.0 2.4979735189827253E-5 0.0 7.493920556948176E-5 0.0 12 0.0 2.4979735189827253E-5 0.0 7.493920556948176E-5 0.0 13 0.0 2.4979735189827253E-5 0.0 7.493920556948176E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 50 8.732381E-5 15.1973505 9 TAGACCG 85 6.5871375E-4 10.06059 5 AGCACCG 185 6.475602E-10 9.758471 5 TATACCG 110 6.82879E-5 9.501669 5 AACGATT 115 1.1140183E-4 9.0850315 17 GGTATCA 3750 0.0 9.051811 1 AGTACCG 95 0.0018218512 9.00158 5 CTAAGAC 395 0.0 8.900297 3 GGACCGG 120 1.7683556E-4 8.706816 6 TATACTG 885 0.0 8.696443 5 GTATTAG 560 0.0 8.659258 1 GTGGTAT 1990 0.0 8.600387 1 TCTAGAC 410 0.0 8.5746765 3 CCGTGCA 200 2.5493136E-8 8.54851 9 TCTATAC 645 0.0 8.544136 3 CTAGACT 325 0.0 8.478412 4 GTATTAA 630 0.0 8.451738 1 TGGACTG 710 0.0 8.431058 5 CTAGGAC 400 0.0 8.31396 3 GTGTTAG 485 0.0 8.23391 1 >>END_MODULE