FastQCFastQC Report
Wed 25 May 2016
SRR1295270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4003245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136630.34129812189860975No Hit
GGTATCAACGCAGAGTACTTTTTTT112810.28179639267644124No Hit
TATCAACGCAGAGTACTTTTTTTTT83030.20740674128113568No Hit
GTGGTATCAACGCAGAGTACATGGG64180.1603199404483113No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55340.13823785454050402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG551.9484601E-413.8289415
TAGGTCG500.001491494613.3103555
ACCGGTC752.0832692E-411.3953398
GGTATCA47100.010.549261
AGCGTCA3100.010.1207773
CTAGCAC3450.09.64518453
TTAGACT3600.09.5073974
ACACCGT1804.2145984E-99.496596
CAGGACG1856.6193024E-99.2504414
GTCGTAG1054.4084093E-49.065321
TAGACTG4400.08.8591655
TCAGGAC5650.08.7501713
CAGCGTC3800.08.50661852
CTTCTAG5550.08.4037961
GACAGTG7050.08.3511947
TTATACT7200.08.3189734
GCTAGAC1606.38842E-68.3189733
GTCCTAG3900.08.2982541
TATACTG9800.08.2462115
TAGACAG6350.08.2347545
Produced by FastQC (version 0.11.5)