FastQCFastQC Report
Wed 25 May 2016
SRR1295269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941468
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26180.1348464151868586No Hit
GTGGTATCAACGCAGAGTACATGGG25690.1323225518010083No Hit
GGTATCAACGCAGAGTACTTTTTTT21720.11187410763401714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1001.4295074E-712.3591
CGACCAT600.005884147711.0814096
ACCGTGT600.005884147711.0814098
GGTATCA17800.09.7739721
GACAGCC1701.6692866E-89.4983517
CGTCAGA1252.7480284E-59.1217072
TCTGGAC2201.6734703E-109.069883
CTAGAAC1901.0541044E-89.0016853
GTTCTAA1606.669361E-78.9127411
ATTAGAC1606.721657E-78.9079173
GTAGCAC1752.6171438E-78.687343
CTTGCAC1752.6171438E-78.687343
TCTAGGT2201.5988917E-98.637983
CTAGGGT1651.0469575E-68.637984
GGACCAT1651.0524018E-68.6348656
GTCTAAG1554.1653057E-68.5868991
CTAGGAC1451.6849897E-58.5188363
TATACTG5500.08.465225
CCAGGAC3600.08.4460253
CTAATAC2252.4374458E-98.4460253
Produced by FastQC (version 0.11.5)