##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295269_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1941468
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.75391919928631	31.0	30.0	33.0	25.0	34.0
2	29.574290176299584	31.0	30.0	33.0	25.0	34.0
3	29.939762592018	31.0	30.0	34.0	25.0	34.0
4	33.30719331969417	35.0	33.0	37.0	26.0	37.0
5	33.4931258202556	35.0	33.0	37.0	27.0	37.0
6	33.79070116015304	35.0	35.0	37.0	28.0	37.0
7	33.6257852305575	35.0	35.0	37.0	27.0	37.0
8	33.6198716641222	35.0	35.0	37.0	27.0	37.0
9	34.674056950719766	38.0	34.0	39.0	26.0	39.0
10	34.56360959850999	38.0	34.0	39.0	26.0	39.0
11	34.979186883327465	38.0	35.0	39.0	27.0	39.0
12	34.996106039347545	38.0	35.0	39.0	27.0	39.0
13	34.88639781855792	38.0	35.0	39.0	27.0	39.0
14	35.02453761792623	38.0	33.0	40.0	23.0	41.0
15	35.66104463220614	39.0	34.0	41.0	27.0	41.0
16	35.71806746235323	39.0	34.0	41.0	27.0	41.0
17	35.51698817595757	39.0	34.0	41.0	25.0	41.0
18	35.46596750500137	39.0	34.0	40.0	25.0	41.0
19	35.54515758178863	39.0	34.0	40.0	25.0	41.0
20	35.5655256743866	39.0	34.0	41.0	25.0	41.0
21	35.554231643272	39.0	34.0	40.0	25.0	41.0
22	35.27544157307769	39.0	34.0	40.0	25.0	41.0
23	34.944094365706775	38.0	33.0	40.0	23.0	41.0
24	35.30715211376134	39.0	34.0	40.0	25.0	41.0
25	33.87249802726597	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0605064995915825
1101	2	0.3123409160913262
1101	3	0.12596818924027886
1101	4	0.14881422865019545
1101	5	0.0608562927251981
1101	6	0.005720944910514447
1101	7	-0.03079960898954681
1101	8	-0.022726781943887886
1101	9	-0.13190179902371568
1101	10	0.1661562088701274
1101	11	0.026114042465941623
1101	12	0.17114450251815327
1101	13	0.10853446834779845
1101	14	0.695877406811725
1101	15	0.46152212059728726
1101	16	0.31931966789182553
1101	17	0.5725302363586664
1101	18	0.5216117585609652
1101	19	0.5231805782725232
1101	20	0.695319401125154
1101	21	0.5614563777605994
1101	22	0.5003216677243572
1101	23	-2.0196085512459305
1101	24	-0.1448259718460818
1101	25	0.1949635752290817
1102	1	0.1123887507001271
1102	2	0.17638756615510687
1102	3	0.10674022349891743
1102	4	0.24377375911244314
1102	5	-0.05558360279809449
1102	6	0.040492857684618855
1102	7	0.09790453059618898
1102	8	0.030767796808468972
1102	9	0.23553730685005547
1102	10	0.7625845633620187
1102	11	0.5100913704134626
1102	12	0.20730209833908475
1102	13	0.379793124121349
1102	14	0.7507629835595893
1102	15	0.4749852652246318
1102	16	0.38311461717202633
1102	17	0.7891466641057292
1102	18	0.7581797443472738
1102	19	0.5705067794509233
1102	20	0.751551558083186
1102	21	0.6887171291424892
1102	22	0.5304855199991607
1102	23	0.5567399271601943
1102	24	0.6360743804590854
1102	25	0.5088488786367265
1103	1	-0.5277564934327685
1103	2	-1.9495472132934744
1103	3	-0.7262601156867134
1103	4	-0.3386561223341005
1103	5	-0.4510183257157365
1103	6	-0.2850195548943262
1103	7	-0.2154011362266104
1103	8	-0.11190851915102229
1103	9	0.48780015956273814
1103	10	0.578507713588948
1103	11	0.32317616278339756
1103	12	0.10192943831614798
1103	13	0.04718577590691808
1103	14	-1.1122781992291664
1103	15	-0.367690041253816
1103	16	-0.35824816205042964
1103	17	0.2657292275415202
1103	18	0.3650469410587931
1103	19	0.3948406229086814
1103	20	0.3644729154717794
1103	21	0.35657192135090554
1103	22	-0.07140443041513578
1103	23	0.7120025537491159
1103	24	0.5373651029244826
1103	25	0.37450300216490007
1104	1	-0.17464922489399726
1104	2	-0.13322764239348928
1104	3	-0.4703423196298999
1104	4	-0.47405170160404
1104	5	-0.4656278106534941
1104	6	-0.35344098748325337
1104	7	-0.3033810776542012
1104	8	-0.29089625945845654
1104	9	-2.180794749076803
1104	10	-0.743634618661865
1104	11	-0.5776577074996183
1104	12	-0.16066535395436432
1104	13	-0.04285906926840255
1104	14	-1.6756723820707649
1104	15	-0.6441229426569208
1104	16	-0.22433262441474966
1104	17	-0.02574070722717181
1104	18	0.11460516974978674
1104	19	0.1454480045840043
1104	20	0.26958130029122174
1104	21	0.32083510068204646
1104	22	0.5122293433246838
1104	23	0.9319851630444163
1104	24	0.46394249587036285
1104	25	0.41135739649643455
1105	1	-0.41453078586588177
1105	2	-0.317500751717418
1105	3	-1.616373579339971
1105	4	-0.7882471129813382
1105	5	-0.5893230805566461
1105	6	-0.33637252267733686
1105	7	-0.4201146045959092
1105	8	-0.2699906339951923
1105	9	0.09774961218045775
1105	10	0.29406346683712314
1105	11	-0.41256649573452364
1105	12	-0.2610165222877612
1105	13	-0.4562660443776281
1105	14	0.4492597016610915
1105	15	0.04664018449448548
1105	16	-0.39843869009693833
1105	17	0.16032035752632368
1105	18	-0.11182463043316915
1105	19	-0.17553599581871993
1105	20	-0.6203795903536076
1105	21	-0.15716740592731782
1105	22	0.00616157807802864
1105	23	0.051254375319857104
1105	24	-0.08517810779893864
1105	25	-0.0443937444100726
1106	1	-1.5126886844685608
1106	2	-1.0508603299291295
1106	3	-1.2305887942928706
1106	4	-1.401301922049253
1106	5	-1.0925944892455561
1106	6	-0.7918624568226704
1106	7	-0.57562913294867
1106	8	-0.7213047655234988
1106	9	-0.3260278922230384
1106	10	-2.014415540182412
1106	11	-1.4164627110224544
1106	12	-0.9786741917709989
1106	13	-0.8767086405971369
1106	14	-0.23465142179187382
1106	15	-0.9261089361085908
1106	16	-1.4226149381581905
1106	17	-0.7251258425264666
1106	18	-0.6807577157077844
1106	19	-0.6077028971333931
1106	20	-0.3994269368092205
1106	21	-0.3326965960291659
1106	22	-0.3764309357106015
1106	23	-0.6579027731358025
1106	24	-0.8989903615903074
1106	25	-1.4567874242200318
1107	1	0.08769819658984446
1107	2	0.20431040362929664
1107	3	0.08811594338207485
1107	4	0.3743432657887382
1107	5	0.052024946952940354
1107	6	0.13435251802120973
1107	7	0.008643213809250483
1107	8	-0.07671431397523776
1107	9	0.44009438295793046
1107	10	0.10370051808208558
1107	11	0.14142790020910923
1107	12	0.03561396035162545
1107	13	0.23549070767892744
1107	14	-0.8477082035365271
1107	15	-0.3676177955172051
1107	16	-0.13456746293075383
1107	17	0.2662600125451604
1107	18	0.4327204493054424
1107	19	0.382228370832145
1107	20	0.42676201005155434
1107	21	0.5795751323895502
1107	22	0.059499367962388305
1107	23	0.6250105776597152
1107	24	0.48466921008407127
1107	25	0.07086649300306647
1108	1	-0.0037737146992213866
1108	2	0.36792061968009904
1108	3	0.037746290845532826
1108	4	-0.3062669966496685
1108	5	-0.281207035866224
1108	6	-0.13034582172772247
1108	7	-0.21930043962628076
1108	8	-0.13786756668866218
1108	9	0.46947110970634043
1108	10	0.3054264612951414
1108	11	0.06896305908060185
1108	12	0.0241957209457766
1108	13	0.0012984000411577767
1108	14	0.14713568189782222
1108	15	0.06935349460326279
1108	16	0.02690993587082602
1108	17	0.392889883775986
1108	18	0.383527401288994
1108	19	0.34749985077719714
1108	20	0.5106875281596359
1108	21	0.5167815003348579
1108	22	0.38266442905730713
1108	23	0.13952402530643582
1108	24	0.32605467633931795
1108	25	-0.5186066997184113
1109	1	-0.8078491315218308
1109	2	-0.7940983378569832
1109	3	-0.8141181273964122
1109	4	-0.7014522770453908
1109	5	-1.131183030210721
1109	6	-1.339515625091373
1109	7	-1.1309104819291562
1109	8	-1.4945749106550466
1109	9	-0.9819067267099229
1109	10	-1.38482686452776
1109	11	-1.3259321792306622
1109	12	-1.1912301494640118
1109	13	-1.376685347520997
1109	14	-0.8566406408389966
1109	15	-1.4524252845652867
1109	16	-1.4315122897957764
1109	17	-1.5250461633095398
1109	18	-2.081134194576734
1109	19	-2.2244689154077193
1109	20	-1.9096013041909927
1109	21	-1.6168827456040233
1109	22	-1.7972834767800379
1109	23	-1.0416221680854747
1109	24	-2.213330665467197
1109	25	-1.20853343941436
1110	1	-0.022641437421928146
1110	2	0.1830758456025201
1110	3	0.0528275313447466
1110	4	0.2197280193614759
1110	5	0.26830874218861567
1110	6	0.2187893746519478
1110	7	0.10937810605108211
1110	8	0.0595758121970249
1110	9	0.3411129824117154
1110	10	0.3337031058947346
1110	11	0.25508905417888883
1110	12	0.17779363495586864
1110	13	0.23204159564991755
1110	14	-2.4839986471020055
1110	15	-0.7389953603452994
1110	16	-0.15696770042551833
1110	17	-0.23025342302687335
1110	18	-2.8510782776938655
1110	19	-0.5182428311295979
1110	20	-2.0043440904787246
1110	21	-0.22101411868731446
1110	22	-1.6670318902964425
1110	23	-0.5246012821819832
1110	24	0.0771834065534307
1110	25	0.08025593637789541
1111	1	0.022423273889287998
1111	2	-1.2323051123232673
1111	3	-0.2906184268397993
1111	4	0.14303444175965296
1111	5	0.06483393237647306
1111	6	0.16718970500092922
1111	7	0.07359173747477143
1111	8	0.12883319545359484
1111	9	-1.6768747567247217
1111	10	-0.3327580630058833
1111	11	-0.20359414287471367
1111	12	0.09489329166893867
1111	13	0.09330602146371092
1111	14	0.9755512847581258
1111	15	0.4706014715821141
1111	16	0.6213962742851038
1111	17	-0.02170208524288597
1111	18	0.19488831652028438
1111	19	0.24107087533419502
1111	20	0.43412711827078
1111	21	-0.44381283438018215
1111	22	-0.050386679062853545
1111	23	0.21407121949995656
1111	24	-0.32664708905628714
1111	25	-1.69332347675671
1112	1	0.3427572387323643
1112	2	0.5169822765137155
1112	3	0.14912537917425084
1112	4	0.5993426185131767
1112	5	0.4684739804750251
1112	6	0.28161386327629856
1112	7	0.2312442514025932
1112	8	0.24668005001603888
1112	9	0.08281567374000076
1112	10	0.8607075413402896
1112	11	0.25973395671351085
1112	12	0.04519087982077963
1112	13	0.2313899517035125
1112	14	0.8124954696780193
1112	15	0.37945450282886384
1112	16	0.48606135785200877
1112	17	-3.068698088564801
1112	18	-0.7072540586484095
1112	19	-0.937619347510882
1112	20	0.07286505037915703
1112	21	-0.12962008549613557
1112	22	-1.4568408383763227
1112	23	-1.0029923034593153
1112	24	-0.17115208922930236
1112	25	0.3236624051077115
1113	1	-0.4248214543105213
1113	2	-0.137286855474251
1113	3	-0.1281384121478979
1113	4	-0.41961787869665557
1113	5	-0.04249527426080846
1113	6	-0.20942910495499945
1113	7	-0.34071126140172936
1113	8	-0.2858711300152521
1113	9	0.2334522461272357
1113	10	0.3341732739572123
1113	11	-0.06759736212974587
1113	12	-8.33093258897577E-4
1113	13	-0.3999194992345849
1113	14	-1.7361201351710065
1113	15	-1.8797015489001296
1113	16	-0.5581511935223418
1113	17	-0.32462642194671787
1113	18	-0.6048794217910114
1113	19	-0.0600585646450611
1113	20	0.31199311948070374
1113	21	-1.6462969541623949
1113	22	-2.126276295344937
1113	23	-0.763849336199641
1113	24	-2.139113600161636
1113	25	-0.38554757147626617
1114	1	0.28037380353730157
1114	2	0.291194181771683
1114	3	-0.04258871306018008
1114	4	0.4105303641714855
1114	5	0.39638207860843266
1114	6	0.38860701765109695
1114	7	0.37072953364797456
1114	8	0.28459541944910427
1114	9	0.6181680141060539
1114	10	0.5270599362575226
1114	11	0.35633270168587217
1114	12	0.42771480730407774
1114	13	0.274578711268866
1114	14	1.12127200942885
1114	15	0.6299640305536798
1114	16	0.4799207354296371
1114	17	-0.33201334114004055
1114	18	0.10881152632754265
1114	19	-0.30350582278397553
1114	20	-1.3618726800420617
1114	21	-0.49399599701018104
1114	22	-1.203850765595412
1114	23	-2.6263182778605767
1114	24	-1.656972945349807
1114	25	-1.273658540507391
1115	1	0.6292025374759547
1115	2	0.5930122225107617
1115	3	0.5576738606508229
1115	4	0.8347502383795131
1115	5	0.7545718017443974
1115	6	0.47449465908675137
1115	7	0.6403906083791213
1115	8	0.7367638864887169
1115	9	0.863186477062392
1115	10	0.9949570368619121
1115	11	0.6752681414001316
1115	12	0.6366966289326683
1115	13	0.5161125923716341
1115	14	1.38177738973544
1115	15	0.9687843332678909
1115	16	0.8370698459433754
1115	17	0.794389760971427
1115	18	1.1174626367403846
1115	19	0.2801258383527454
1115	20	0.9144675441828127
1115	21	-0.11129215513624757
1115	22	0.9490623955074824
1115	23	0.9535281548628021
1115	24	1.1201478514518044
1115	25	1.132358982956724
1116	1	0.690153057076472
1116	2	0.7670335326859288
1116	3	0.6484448266025957
1116	4	0.8913681620461205
1116	5	0.8478011113095576
1116	6	0.7187199185990565
1116	7	0.8035133348172963
1116	8	0.8438684453955503
1116	9	0.8894671297610941
1116	10	0.8888944203402502
1116	11	0.7330703732255159
1116	12	0.6839856782172617
1116	13	0.25028390262743017
1116	14	1.1532690351912152
1116	15	0.9376907729233466
1116	16	0.9335883116826409
1116	17	1.1760331227295353
1116	18	1.0880832248123937
1116	19	1.1858513612878028
1116	20	0.963514050565891
1116	21	0.5051159379663588
1116	22	0.5947304561147604
1116	23	-0.10242470409763627
1116	24	0.19227581202063249
1116	25	-0.2707594955447732
1201	1	0.25318143775558966
1201	2	0.46458732178500384
1201	3	0.3820605360710516
1201	4	0.24854218843439213
1201	5	0.2812662696308621
1201	6	0.16032884656793556
1201	7	0.124333684041531
1201	8	0.14169506823471067
1201	9	0.37941328132969687
1201	10	0.738963915627366
1201	11	0.4614046096407094
1201	12	0.5206830925622228
1201	13	0.43851629419247473
1201	14	0.8361675830419131
1201	15	0.5042310995454002
1201	16	0.3372930111926564
1201	17	0.32889691186131387
1201	18	0.18352250495998845
1201	19	0.6671057762716543
1201	20	0.6948078358402725
1201	21	0.5832400957770432
1201	22	0.7154294826111496
1201	23	0.14593616804632603
1201	24	0.6423915706953593
1201	25	0.7754014712988493
1202	1	0.34015153404060783
1202	2	0.5841585692441136
1202	3	0.3462767477728157
1202	4	0.3748397082214794
1202	5	0.23518262071691254
1202	6	0.1608228285348119
1202	7	0.14837615614857924
1202	8	0.1701952812235774
1202	9	0.6757351401927139
1202	10	-0.1079831431261482
1202	11	0.2755074777021562
1202	12	0.20750603822459368
1202	13	0.24765192624450094
1202	14	0.8791443068614058
1202	15	0.4678887398941356
1202	16	0.55664939649742
1202	17	-1.73865578068677
1202	18	-0.013884026295322371
1202	19	0.4350372826424902
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2210	17	0.683948844049354
2210	18	0.650925743043338
2210	19	0.8057274024654575
2210	20	0.9774398667332846
2210	21	0.941421484875022
2210	22	1.2196026604091301
2210	23	1.466521229693285
2210	24	1.2057796038865263
2210	25	1.0253532586583276
2211	1	0.25038380020507844
2211	2	-0.3593587164612231
2211	3	0.1545066756496034
2211	4	0.4653649199272323
2211	5	0.4516288270958668
2211	6	0.30941697415990177
2211	7	0.3461612122056863
2211	8	0.3440680270220753
2211	9	0.8681201624176822
2211	10	1.0363147786109224
2211	11	0.6787038657780116
2211	12	0.30445228195207363
2211	13	0.25419759203747816
2211	14	-0.48809419785792585
2211	15	0.2933042915671109
2211	16	0.4685694203172375
2211	17	0.6475260169150587
2211	18	0.6079299839138272
2211	19	0.7459167705246088
2211	20	0.8171337212050318
2211	21	0.880878934194385
2211	22	0.24427220567800134
2211	23	0.5861405677545832
2211	24	-0.02839769527020053
2211	25	0.6605985964076382
2212	1	-0.012654748547756611
2212	2	-0.8852667042282008
2212	3	-0.24025708556143144
2212	4	0.14322965769063956
2212	5	0.17287284342961584
2212	6	0.15644782052783768
2212	7	0.17777225680029574
2212	8	0.2605203001200209
2212	9	-2.0939496505920445
2212	10	0.09638857058489947
2212	11	0.04176894596984937
2212	12	0.12936808409217804
2212	13	0.08268273706223539
2212	14	0.7752367228431325
2212	15	0.5061300251087815
2212	16	0.6605322732962406
2212	17	0.6010373739493104
2212	18	0.33419768688496987
2212	19	-0.16969296027523484
2212	20	0.5802102256992185
2212	21	0.3899082668422622
2212	22	-1.7650573907059055
2212	23	-0.18865636902918936
2212	24	0.4185330381324306
2212	25	-0.2941431279254516
2213	1	0.3213387073668805
2213	2	0.6214790912227315
2213	3	0.1558643666824686
2213	4	0.5166837507084168
2213	5	0.4522754722340778
2213	6	0.2365068556361365
2213	7	0.3066993739399422
2213	8	0.33444275150552727
2213	9	0.7515935875444413
2213	10	0.6303492244435134
2213	11	0.16234453505352775
2213	12	0.1981216875924403
2213	13	0.20384455280532876
2213	14	-1.5502479613677949
2213	15	-0.23124569440979315
2213	16	0.032624984322801254
2213	17	-2.101429503922212
2213	18	-0.8204282407881109
2213	19	-0.4320365866230844
2213	20	0.31526456068317543
2213	21	0.03752267448481916
2213	22	0.4374126019646454
2213	23	0.8784236207284906
2213	24	0.8466985761677819
2213	25	-0.7924788585799689
2214	1	0.42109904986769564
2214	2	0.3606014328859075
2214	3	0.30431240986735375
2214	4	0.5588913918271317
2214	5	0.5867047151025204
2214	6	0.6101805956891866
2214	7	0.5401909277581751
2214	8	0.3162066772349732
2214	9	0.8787342176342534
2214	10	0.809330331056195
2214	11	0.2063670582049113
2214	12	0.143611240386889
2214	13	0.19082626437869266
2214	14	1.1484932550685159
2214	15	0.7881537657791426
2214	16	0.6875821717210044
2214	17	0.5665401549730404
2214	18	0.5596150371680579
2214	19	-0.7404152920072349
2214	20	0.22915073500492156
2214	21	0.672777921355312
2214	22	0.9582081252556378
2214	23	1.3691572518886161
2214	24	1.057765866409298
2214	25	1.0075874544173757
2215	1	0.04886551173026987
2215	2	0.5549313211517237
2215	3	0.4751694577266896
2215	4	0.6125469912493244
2215	5	0.5855430875106862
2215	6	0.5010204321811997
2215	7	0.5166195969786074
2215	8	0.3880466729880112
2215	9	0.5803859221463696
2215	10	0.8438850847185009
2215	11	0.570550230740487
2215	12	0.4557719375590281
2215	13	0.38190387479269106
2215	14	1.2751099640650878
2215	15	0.6990709696025235
2215	16	0.6189483519430183
2215	17	0.8048302782746006
2215	18	0.6350244358039987
2215	19	-0.03261038397349125
2215	20	-1.4640426721472934
2215	21	-1.5845920923076093
2215	22	0.11447951722496441
2215	23	0.7188378916150242
2215	24	0.9566099666263952
2215	25	0.5390282358581615
2216	1	0.4632651561372825
2216	2	0.17720313923783593
2216	3	0.3783545883252337
2216	4	0.6972628092886453
2216	5	0.7857538699322291
2216	6	0.4998937638769334
2216	7	0.6267942352446241
2216	8	0.5881362730628723
2216	9	0.7865072682529757
2216	10	-1.0308958863778699
2216	11	-0.034775008173554056
2216	12	0.25244012485359946
2216	13	0.517269000946321
2216	14	0.16799553096686992
2216	15	0.2801297945080705
2216	16	0.5908866846330127
2216	17	0.9112751672102775
2216	18	0.6987085662972703
2216	19	-0.7104868855935464
2216	20	-0.4007323821752493
2216	21	-0.3068112732740289
2216	22	-0.04106129993706986
2216	23	-3.6554794049425077
2216	24	-1.3990913352650338
2216	25	-0.3415259745265615
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	11.0
4	19.0
5	33.0
6	49.0
7	155.0
8	163.0
9	430.0
10	1203.0
11	2380.0
12	4419.0
13	6821.0
14	9315.0
15	11663.0
16	13565.0
17	14652.0
18	15204.0
19	15780.0
20	15965.0
21	16335.0
22	17010.0
23	17749.0
24	18962.0
25	20677.0
26	23024.0
27	26395.0
28	30575.0
29	36685.0
30	44851.0
31	54531.0
32	70037.0
33	92565.0
34	123232.0
35	166604.0
36	232220.0
37	340034.0
38	498152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.42449548099223	20.65969832729957	13.508980023372134	23.406826168336067
2	15.899803823787604	21.043865578081018	40.11283223224626	22.94349836588512
3	18.29027268708531	26.05932487199277	29.977701135449113	25.672701305472806
4	12.744325143732796	17.59084355874057	37.72786246994725	31.93696882757938
5	13.290459553329418	37.91340733182863	34.95099403271187	13.845139082130084
6	30.738764395143797	38.3315491271471	17.834523204812104	13.095163272896999
7	26.29337325717442	32.78091156803943	23.059830636224394	17.86588453856176
8	24.32755221018344	36.91735145865976	20.623879039001842	18.13121729215496
9	24.866448477553863	17.13936966978974	21.53647110711739	36.457710745539
10	15.175927810221046	29.93815027859423	33.564280175889174	21.321641735295557
11	32.22957750324702	25.244086967139257	23.88505153762811	18.64128399198561
12	21.376113137990473	27.230371525098217	31.083222487473815	20.310292849437495
13	26.486979414878682	24.036085143214844	26.85272531962194	22.624210122284534
14	21.548390952897194	23.273268012382676	27.75111604842599	27.427224986294142
15	22.94753221593046	30.889937706421545	24.07809110629067	22.08443897135732
16	22.515503710225776	30.6911380653245	25.54313861968164	21.250219604768088
17	20.88483620746505	30.06272050580322	27.851789188208915	21.20065409852282
18	20.86057913251711	29.711711451788076	28.802527805656137	20.62518161003868
19	21.556609824414263	28.683228040964337	29.02870172268035	20.731460411941047
20	22.464066385424303	28.298600468073293	28.56184661764759	20.675486528854815
21	22.86937811046507	28.50256203673034	27.68241150322811	20.94564834957648
22	22.26823806268446	28.04614245700547	28.719774521809327	20.96584495850074
23	22.51824375668122	28.299679301418028	28.132400860348007	21.049676081552747
24	21.994827728321795	27.91445145070887	28.299538410814375	21.791182410154963
25	22.44056627366456	28.13475683800458	28.0957230405111	21.328953847819754
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1794.0
1	1794.0
2	1901.0
3	2008.0
4	2008.0
5	2008.0
6	2803.5
7	3599.0
8	3599.0
9	3599.0
10	5491.5
11	7384.0
12	7384.0
13	7384.0
14	12180.5
15	16977.0
16	16977.0
17	16977.0
18	26449.0
19	35921.0
20	35921.0
21	35921.0
22	51760.0
23	67599.0
24	67599.0
25	67599.0
26	87923.5
27	108248.0
28	108248.0
29	108248.0
30	131129.0
31	154010.0
32	154010.0
33	154010.0
34	174035.0
35	194060.0
36	194060.0
37	194060.0
38	209538.0
39	225016.0
40	225016.0
41	225016.0
42	230788.0
43	236560.0
44	236560.0
45	236560.0
46	230788.0
47	225016.0
48	225016.0
49	225016.0
50	215586.5
51	206157.0
52	206157.0
53	206157.0
54	188078.0
55	169999.0
56	169999.0
57	169999.0
58	147113.5
59	124228.0
60	124228.0
61	124228.0
62	102623.0
63	81018.0
64	81018.0
65	81018.0
66	62968.5
67	44919.0
68	44919.0
69	44919.0
70	33240.0
71	21561.0
72	21561.0
73	21561.0
74	15369.0
75	9177.0
76	9177.0
77	9177.0
78	6494.0
79	3811.0
80	3811.0
81	3811.0
82	2657.5
83	1504.0
84	1504.0
85	1504.0
86	1058.5
87	613.0
88	613.0
89	613.0
90	414.5
91	216.0
92	216.0
93	216.0
94	136.5
95	57.0
96	57.0
97	57.0
98	36.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0798880022745675
2	0.01833664010944296
3	0.02894716781322175
4	0.03677629505096144
5	0.04573858544153187
6	0.018645684605669524
7	0.02261175564057713
8	0.03147103119907204
9	0.033170775928318165
10	0.024363007785861007
11	0.023641903961332353
12	0.017203476956612212
13	0.02590823026699384
14	0.03564313189813069
15	0.03389187975284681
16	0.024775067114163097
17	0.02451753003397429
18	0.02554767835472951
19	0.018800206853782808
20	0.0164308657160458
21	0.012310272433024906
22	0.01797608819717863
23	0.02024241450284012
24	0.01792458078114087
25	0.10935024424816686
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1941468.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.41985152429315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69790121128567	73.03189798663594
2	7.313104867033844	11.908638249109625
3	1.4953256252100167	3.652475711552112
4	0.5278298837161373	1.7190332304901124
5	0.26783127731072176	1.0903391416100376
6	0.17428008161749156	0.8513915021362715
7	0.10504587503410903	0.5986973683961627
8	0.07634574844615985	0.4972847602397895
9	0.06046229453760559	0.4430547939662936
>10	0.2622976735845922	3.7323837391994643
>50	0.013989004745414965	0.7678557427073542
>100	0.004634898254556365	0.7238830371292286
>500	3.8062368947836895E-4	0.2185334367423729
>1k	5.709355342175476E-4	0.7645313000852229
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2618	0.1348464151868586	No Hit
GTGGTATCAACGCAGAGTACATGGG	2569	0.1323225518010083	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2172	0.11187410763401714	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.150741603776112E-5	0.0	0.0	0.0
3	0.0	5.150741603776112E-5	0.0	5.150741603776112E-5	0.0
4	0.0	5.150741603776112E-5	0.0	5.150741603776112E-5	0.0
5	0.0	5.150741603776112E-5	0.0	5.150741603776112E-5	0.0
6	0.0	5.150741603776112E-5	0.0	5.150741603776112E-5	0.0
7	0.0	5.150741603776112E-5	0.0	5.150741603776112E-5	0.0
8	0.0	5.150741603776112E-5	0.0	5.150741603776112E-5	0.0
9	0.0	5.150741603776112E-5	0.0	1.0301483207552224E-4	0.0
10	5.150741603776112E-5	5.150741603776112E-5	0.0	1.0301483207552224E-4	0.0
11	5.150741603776112E-5	5.150741603776112E-5	0.0	1.0301483207552224E-4	0.0
12	5.150741603776112E-5	1.5452224811328335E-4	0.0	1.0301483207552224E-4	0.0
13	5.150741603776112E-5	1.5452224811328335E-4	0.0	1.0301483207552224E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAG	100	1.4295074E-7	12.359	1
CGACCAT	60	0.0058841477	11.081409	6
ACCGTGT	60	0.0058841477	11.081409	8
GGTATCA	1780	0.0	9.773972	1
GACAGCC	170	1.6692866E-8	9.498351	7
CGTCAGA	125	2.7480284E-5	9.121707	2
TCTGGAC	220	1.6734703E-10	9.06988	3
CTAGAAC	190	1.0541044E-8	9.001685	3
GTTCTAA	160	6.669361E-7	8.912741	1
ATTAGAC	160	6.721657E-7	8.907917	3
GTAGCAC	175	2.6171438E-7	8.68734	3
CTTGCAC	175	2.6171438E-7	8.68734	3
TCTAGGT	220	1.5988917E-9	8.63798	3
CTAGGGT	165	1.0469575E-6	8.63798	4
GGACCAT	165	1.0524018E-6	8.634865	6
GTCTAAG	155	4.1653057E-6	8.586899	1
CTAGGAC	145	1.6849897E-5	8.518836	3
TATACTG	550	0.0	8.46522	5
CCAGGAC	360	0.0	8.446025	3
CTAATAC	225	2.4374458E-9	8.446025	3
>>END_MODULE