FastQCFastQC Report
Wed 25 May 2016
SRR1295269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941468
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73720.3797126710303749No Hit
GGTATCAACGCAGAGTACTTTTTTT61540.3169766382963819No Hit
TATCAACGCAGAGTACTTTTTTTTT47060.2423938998737038No Hit
GTGGTATCAACGCAGAGTACATGGG30650.1578702301557378No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28110.1447873464821465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG550.003049336412.1012485
GGTATCA24650.011.0840291
CGGAATA700.001480456210.8669864
TAGAACT2800.010.18784
TACCGTG750.002657625810.1289387
AGCGTCA1803.8744474E-1010.0371283
TAGTACC1451.5959995E-79.8364964
CTACTAG1201.6589085E-59.5199071
GTCCTAG1304.127278E-69.5199061
GCTCTAG1304.127278E-69.5199061
TACGGAA900.00110432099.5088592
TTAGTAC800.0044817129.5088593
TGGTATC10100.09.5088592
TCCGAAT800.00453249649.49587913
CGCTATC800.00453249649.49587912
ACCGTGT1106.869565E-59.4958798
ATCGCTA800.00453249649.49587910
GTGGTAT10250.09.4734681
CAGCGTC1951.6534614E-99.2650412
CGAACTC1054.5122587E-49.043694510
Produced by FastQC (version 0.11.5)