FastQCFastQC Report
Wed 25 May 2016
SRR1295268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976343
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG72210.18159902201595787No Hit
GTATCAACGCAGAGTACTTTTTTTT54020.13585347139318715No Hit
GGTATCAACGCAGAGTACTTTTTTT43190.10861739040118018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.010.6916731
GTGGTAT16800.010.4683541
CCCGACG750.002653142210.13148212
TGGTATC17850.09.954322
CGACGTT800.0045253479.49814414
CTAGTAC1804.2085958E-88.9743093
CCTATAC4050.08.9156523
GTCTTAG3200.08.9122481
TATACTG7350.08.6623135
GTATTAG6150.08.6562321
CGCAAGT1107.1615085E-48.63478512
CCGCAAC1451.6968843E-58.5147217
CGTTCCG1901.0146323E-78.49887510
TCCTATA4050.08.446092
TCTATAC4350.08.300783
TATACCG1150.00110809268.26320655
CGGACTG1150.00110809268.26320655
CGCAACC1150.00111460018.25832118
TCTAGAC3001.8189894E-128.2352483
CCAGTAC5200.08.2230653
Produced by FastQC (version 0.11.5)