FastQCFastQC Report
Wed 25 May 2016
SRR1295268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976343
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT154680.389000647076975No Hit
GGTATCAACGCAGAGTACTTTTTTT127710.3211745063240269No Hit
TATCAACGCAGAGTACTTTTTTTTT94080.23659930745411048No Hit
GTGGTATCAACGCAGAGTACATGGG79160.1990773934743557No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57990.14583751954999857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2950.011.9241683
GGTATCA51150.010.9972111
GTGGTAT22450.010.4294031
GCAGCGT3400.010.3577041
TGGTATC21650.010.3194922
CAGCGTC3800.09.7572932
TAGGACC2106.548362E-119.5071074
CTAGGAC3550.09.3732033
GTATTAG4800.09.3196011
TCGCGTT950.00183094718.99665210
GCGAGCG850.0074617428.9378519
GTGCTAG4000.08.8040491
GTCGTAG1304.27666E-58.7857451
CCTATAC3050.08.7278363
TGCACGG1554.168809E-68.5870645
TACATCG1356.7438275E-58.4507615
TAGACAG4400.08.4267545
GTGTTAG4950.08.2680661
TGTACCG1150.00110300398.2670495
ACAATAC4050.08.2160183
Produced by FastQC (version 0.11.5)