FastQCFastQC Report
Wed 25 May 2016
SRR1295267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059745
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG49500.16177818739796943No Hit
GTATCAACGCAGAGTACTTTTTTTT45680.14929348687554028No Hit
GGTATCAACGCAGAGTACTTTTTTT37700.12321288211926157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCG500.001498182313.3022895
TGGTATC13900.011.4828642
GTGGTAT14600.011.2001191
GGTATCA31750.010.869511
GGGTCGC856.604245E-410.05759513
GTCTTAG2350.09.7093551
TATACCG800.0045111499.5016365
AGTACCG800.0045111499.5016365
AGCGCAA900.00111524169.49899510
TATACTG7200.09.2377015
TAGTACA3250.09.0630984
GGTGTCG1054.5008402E-49.0463668
CTATACT4850.08.815954
GTGTTAG3900.08.77576351
CGGATAG1304.3623222E-58.77074055
TAGGACC1304.3623222E-58.77074054
TAGTACC1951.6456397E-88.77074054
GTATTAT5400.08.6267931
TAGACAG3350.08.5089275
TCTATAC3700.08.4744313
Produced by FastQC (version 0.11.5)