##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295267_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3059745
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.84653459683732	31.0	30.0	33.0	25.0	34.0
2	29.678474840223615	31.0	30.0	34.0	25.0	34.0
3	30.14809861606114	31.0	30.0	34.0	25.0	34.0
4	33.40613776638249	35.0	33.0	37.0	27.0	37.0
5	33.71096186120085	35.0	35.0	37.0	28.0	37.0
6	33.71342056282468	35.0	35.0	37.0	28.0	37.0
7	33.68791909129683	35.0	35.0	37.0	28.0	37.0
8	33.6618688812303	35.0	35.0	37.0	27.0	37.0
9	34.674664718791924	38.0	34.0	39.0	26.0	39.0
10	34.590508686181366	38.0	34.0	39.0	26.0	39.0
11	34.97534042869586	38.0	35.0	39.0	27.0	39.0
12	34.9938932819565	38.0	35.0	39.0	27.0	39.0
13	34.87791956519252	38.0	35.0	39.0	27.0	39.0
14	35.03385282106842	39.0	33.0	41.0	22.0	41.0
15	35.64607802284177	39.0	34.0	41.0	27.0	41.0
16	35.72006458054511	39.0	34.0	41.0	27.0	41.0
17	35.53909361727857	39.0	34.0	41.0	25.0	41.0
18	35.50819659808252	39.0	34.0	41.0	25.0	41.0
19	35.56958210569835	39.0	34.0	41.0	25.0	41.0
20	35.56781954051726	39.0	34.0	41.0	25.0	41.0
21	35.54855486323207	39.0	34.0	41.0	25.0	41.0
22	35.289100235477136	39.0	34.0	40.0	24.0	41.0
23	34.962393271334705	39.0	33.0	40.0	23.0	41.0
24	35.308483876924385	39.0	34.0	41.0	25.0	41.0
25	33.90258599981371	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38434572485480345
1101	2	0.5789783893795111
1101	3	0.39787252738435086
1101	4	0.41080969910277076
1101	5	0.3157030071238509
1101	6	0.2556536945892134
1101	7	0.24163061004657038
1101	8	0.1600722628107505
1101	9	0.16392366776841527
1101	10	0.4991418229535114
1101	11	0.37777704183307037
1101	12	0.5235041368237034
1101	13	0.48121675458190794
1101	14	0.8522115707598701
1101	15	0.6517428241759475
1101	16	0.49552189892136767
1101	17	0.8051012980746606
1101	18	0.7151173356936482
1101	19	0.7430437040715177
1101	20	0.8092312189143414
1101	21	0.8439792334858964
1101	22	0.7745778680181772
1101	23	-1.619368974045912
1101	24	0.0943343630184259
1101	25	0.4864736465139856
1102	1	0.29002094165923253
1102	2	0.35410388262403814
1102	3	0.28647535066498975
1102	4	0.2900936958820992
1102	5	0.195920046843284
1102	6	0.07183610931517848
1102	7	-0.020873353940615402
1102	8	0.1059930471053292
1102	9	0.2857630095710846
1102	10	0.9203378385419825
1102	11	0.5511383930024749
1102	12	0.38045627094061274
1102	13	0.4374338793623238
1102	14	0.8667524702942799
1102	15	0.6847522654595295
1102	16	0.41845454922258085
1102	17	0.8420629215038815
1102	18	0.825694264212828
1102	19	0.7383885155700796
1102	20	0.8332988268686066
1102	21	0.7345730254251279
1102	22	0.5915807735136482
1102	23	0.6896625371658871
1102	24	0.7663809884019486
1102	25	0.6338724380982512
1103	1	-0.489284299496628
1103	2	-1.8227747143482844
1103	3	-0.6129749888028755
1103	4	-0.18771509583691426
1103	5	-0.35905954150425856
1103	6	-0.3300781273828832
1103	7	-0.26094040942460595
1103	8	-0.14455351249829107
1103	9	0.4503362002840561
1103	10	0.5263160934902729
1103	11	0.3356190000469468
1103	12	0.1685180732840763
1103	13	-0.11743860123801397
1103	14	-1.149777406313909
1103	15	-0.3570693708082544
1103	16	-0.18437039665783317
1103	17	0.3143658051159761
1103	18	0.3356433000335812
1103	19	0.35395002248724694
1103	20	0.4714946396094035
1103	21	0.4686221843611236
1103	22	-0.06428924226756294
1103	23	0.844059528091897
1103	24	0.7033952216638468
1103	25	0.3774684340452552
1104	1	-0.20211623448420468
1104	2	-0.21996699203566195
1104	3	-0.5261763323280277
1104	4	-0.45616256242055186
1104	5	-0.42192582996383976
1104	6	-0.43673874377984134
1104	7	-0.27362523327826693
1104	8	-0.10333813014414517
1104	9	-2.1317936019182184
1104	10	-0.8140614120302061
1104	11	-0.7174733777438362
1104	12	-0.2600108327709165
1104	13	-0.21881365331179126
1104	14	-2.055153768168161
1104	15	-0.752753357132967
1104	16	-0.34388330791020394
1104	17	-0.01653253382842479
1104	18	-0.014848737353432284
1104	19	0.08002983038610978
1104	20	0.1814369692618385
1104	21	0.3578441086599824
1104	22	0.4936948090243476
1104	23	0.8375740274313515
1104	24	0.4585167590295569
1104	25	0.08946192688885901
1105	1	-0.31994445345479505
1105	2	-0.22923240412752577
1105	3	-1.5488586224418164
1105	4	-0.6457844689062568
1105	5	-0.6406363403336712
1105	6	-0.3093390150810791
1105	7	-0.37457768879011155
1105	8	-0.27873551254070605
1105	9	0.06529587285786675
1105	10	0.23550109776688544
1105	11	-0.41848637435132474
1105	12	-0.2525889693477694
1105	13	-0.33390109779197985
1105	14	0.439650516805969
1105	15	-0.017011088664716567
1105	16	-0.41888816405234763
1105	17	0.16327420778232238
1105	18	0.0500355257823486
1105	19	-0.05252392550062268
1105	20	-0.5831470789539352
1105	21	-0.15824980698668156
1105	22	-0.11897496402599472
1105	23	0.12490276361678809
1105	24	-0.033571880204434024
1105	25	0.2185455050229379
1106	1	-1.747993680156501
1106	2	-1.1105445915918715
1106	3	-1.2011588455199806
1106	4	-1.5125715925356218
1106	5	-1.1113345120369758
1106	6	-0.980142332532715
1106	7	-0.8824630297613538
1106	8	-0.8527784796769993
1106	9	-0.49202242860059897
1106	10	-2.0633861671059748
1106	11	-1.3616182619388084
1106	12	-1.1132634758807782
1106	13	-1.238825144988553
1106	14	-0.3452753795394017
1106	15	-1.1083587443768153
1106	16	-1.4830680862307233
1106	17	-1.032322359095879
1106	18	-0.8290160772722928
1106	19	-0.7981821634585344
1106	20	-0.5771546565296646
1106	21	-0.5436692686876654
1106	22	-0.43532409512277326
1106	23	-0.8262460692555678
1106	24	-1.0920208181241762
1106	25	-1.7250235882253122
1107	1	0.014782227206129761
1107	2	0.2024274162008055
1107	3	0.04176903611047322
1107	4	0.2011406052439071
1107	5	0.007756114410007342
1107	6	0.132081308479421
1107	7	-0.015295803871723024
1107	8	-0.024521192109624224
1107	9	0.5139088844308333
1107	10	0.11756789357534103
1107	11	0.16473126556611817
1107	12	0.0453021433639762
1107	13	0.19854384803930003
1107	14	-0.8763511888755886
1107	15	-0.3920643912134949
1107	16	-0.044628788940570985
1107	17	0.13898165884742752
1107	18	0.3332928971390956
1107	19	0.32080958083221134
1107	20	0.454892004438598
1107	21	0.42696501280511256
1107	22	-0.18587027641750353
1107	23	0.5724889997765388
1107	24	0.4451087659907813
1107	25	0.17285329778543002
1108	1	-0.1329632535641565
1108	2	0.20436288852360818
1108	3	-0.12231998406821276
1108	4	-0.4757787140841998
1108	5	-0.3907251287729636
1108	6	-0.34805700388341165
1108	7	-0.21825623350962076
1108	8	-0.2752835153000177
1108	9	0.24511929948918265
1108	10	0.25640136731510665
1108	11	-0.036606358295166785
1108	12	-0.06723096377931626
1108	13	-0.2311047355494864
1108	14	-0.019268382186659494
1108	15	-0.09390089576895377
1108	16	-0.15463862220240543
1108	17	0.12481004042417965
1108	18	0.14612573688464892
1108	19	0.09979590607355249
1108	20	0.1768742814709796
1108	21	0.25690761265487083
1108	22	0.1813763583030905
1108	23	-0.044600724262757296
1108	24	0.043226877149947995
1108	25	-0.5241728189653543
1109	1	-0.877461567198953
1109	2	-0.9313901466726762
1109	3	-0.9551376429746377
1109	4	-0.7108727426938728
1109	5	-1.0286147904267011
1109	6	-1.4147057245420385
1109	7	-1.0215276227641041
1109	8	-1.4972106602323976
1109	9	-1.175756217427704
1109	10	-1.3113133902370748
1109	11	-1.4973125624801398
1109	12	-1.401229317827159
1109	13	-1.3151814667304436
1109	14	-0.8685045670868732
1109	15	-1.4399330579900464
1109	16	-1.4728071903677247
1109	17	-1.6018245872383687
1109	18	-2.1672641240724886
1109	19	-2.1741548835730953
1109	20	-1.9778459552091903
1109	21	-1.7117082197719924
1109	22	-1.9173481905974015
1109	23	-1.242073167984735
1109	24	-2.258943434752851
1109	25	-1.4005471482394185
1110	1	0.005571188579668984
1110	2	0.06595843786548627
1110	3	-0.0664310980067171
1110	4	0.21721495483222242
1110	5	0.13766077884883288
1110	6	0.11608885735156349
1110	7	0.029407810845434312
1110	8	0.11354988255553877
1110	9	0.22874741332466186
1110	10	0.18811470286039622
1110	11	0.12441158744736924
1110	12	0.10687218761452044
1110	13	0.16573987657313438
1110	14	-2.3050978361573655
1110	15	-0.687591939977338
1110	16	-0.28144690176652887
1110	17	-0.48768031727104955
1110	18	-2.883683283099785
1110	19	-0.5797458057936709
1110	20	-1.9208202287378242
1110	21	-0.29246465240785824
1110	22	-1.7929081800064353
1110	23	-0.5909315097300265
1110	24	0.008223797624062001
1110	25	-0.10899475451181218
1111	1	-0.11126951733895041
1111	2	-1.2082003667833128
1111	3	-0.22223721172272448
1111	4	0.15064992654788512
1111	5	0.02432418037824391
1111	6	0.3029048178562519
1111	7	0.09426337415258956
1111	8	0.18315569666309983
1111	9	-1.5833766395827311
1111	10	-0.06978601668662776
1111	11	0.02803376036579408
1111	12	0.0378391245507288
1111	13	0.18528971689913476
1111	14	1.1292078388269502
1111	15	0.6246856521225155
1111	16	0.5573412557225979
1111	17	0.11944981636297314
1111	18	0.4930164642069812
1111	19	0.15010092879867187
1111	20	0.5019393327738513
1111	21	-0.201901879299065
1111	22	0.13040545772151546
1111	23	0.3160841581967233
1111	24	-0.37631633517170116
1111	25	-1.5330172751707352
1112	1	0.3046411009406178
1112	2	0.4901465298472196
1112	3	0.06640305072775377
1112	4	0.4342156860925286
1112	5	0.3504320310377693
1112	6	0.30283254602437637
1112	7	0.22003268654648167
1112	8	0.15954661483166177
1112	9	-0.08878172235013437
1112	10	0.585667122597691
1112	11	0.32711500682872696
1112	12	0.28519057447672225
1112	13	0.20034623517216943
1112	14	0.6634592454918788
1112	15	0.32745626506927294
1112	16	0.3235952757369631
1112	17	-3.50624637394219
1112	18	-0.9115961111597812
1112	19	-1.0889232756330074
1112	20	-0.06421609145670004
1112	21	-0.428225627176829
1112	22	-1.4690944892224564
1112	23	-1.3002236660708277
1112	24	-0.15162899161896348
1112	25	0.20124447061743211
1113	1	-0.14211714300850176
1113	2	0.07130442985754826
1113	3	-0.025443381213975158
1113	4	-0.29280436643890795
1113	5	0.015984781781320123
1113	6	-0.1358606705906098
1113	7	-0.20014621479195682
1113	8	-0.04685883625061393
1113	9	0.37151042114309973
1113	10	0.53245450523778
1113	11	0.07049924596638135
1113	12	0.12980978290629253
1113	13	-0.19405770478780937
1113	14	-1.3162686604790963
1113	15	-1.565319742234827
1113	16	-0.27934686300205414
1113	17	-0.055359381965047305
1113	18	-0.4342384076567001
1113	19	-0.007877840745713627
1113	20	0.4374028951098481
1113	21	-1.4576925118213495
1113	22	-1.7634509893487191
1113	23	-0.63412367026271
1113	24	-2.2862589455798314
1113	25	-0.606043218234511
1114	1	0.32396296001798675
1114	2	0.4582940279751959
1114	3	0.13316467217469707
1114	4	0.46421182796930793
1114	5	0.36263466525397803
1114	6	0.40770913886229465
1114	7	0.4042183995041526
1114	8	0.3295339746256545
1114	9	0.7547487362216927
1114	10	0.5796922245757514
1114	11	0.558103023491185
1114	12	0.5280110770815512
1114	13	0.48240551950904376
1114	14	1.2547727304327694
1114	15	0.7022130892311367
1114	16	0.5603526849169853
1114	17	-0.05183760834071194
1114	18	0.21513727754255996
1114	19	-0.08326617050578733
1114	20	-1.3579939568623445
1114	21	-0.5466866957169145
1114	22	-1.1502589137168044
1114	23	-2.617712729330087
1114	24	-1.7903790890936122
1114	25	-1.110390835602793
1115	1	0.43237053016574833
1115	2	0.5502820140290758
1115	3	0.3257434522303022
1115	4	0.5674481023290028
1115	5	0.5691987249760615
1115	6	0.4310615088339276
1115	7	0.5447789127772893
1115	8	0.6184910337431404
1115	9	0.682370582326584
1115	10	1.0171979466998664
1115	11	0.5571538278724688
1115	12	0.6181673386735582
1115	13	0.4546271805631221
1115	14	1.244770178096772
1115	15	0.6947114651150059
1115	16	0.7372053271005399
1115	17	0.6136179380728137
1115	18	0.873218257859655
1115	19	0.24673548606257611
1115	20	0.9042260287108093
1115	21	-0.1397924570759912
1115	22	1.01506948871085
1115	23	1.1020904235828581
1115	24	1.0581249402703747
1115	25	1.0352622284340214
1116	1	0.7531155291923533
1116	2	0.88616119145874
1116	3	0.6359748535769434
1116	4	1.0418155189871072
1116	5	0.8215491391902532
1116	6	0.7682515901645601
1116	7	0.7909485754171754
1116	8	0.7696042200178042
1116	9	1.090670818450043
1116	10	1.006725554913693
1116	11	0.8665983865569089
1116	12	0.8764152305040653
1116	13	0.2933285421507108
1116	14	1.380590280495845
1116	15	0.9825341916184627
1116	16	1.0021246430456046
1116	17	1.1691697101647378
1116	18	1.3399762752988025
1116	19	1.3617181698708904
1116	20	1.2447620462835118
1116	21	0.7021029575074138
1116	22	0.8007529536027178
1116	23	-0.0016822696908889156
1116	24	0.3754358939893976
1116	25	-0.16245124636139963
1201	1	0.3696285850947234
1201	2	0.5399997897832982
1201	3	0.4933751812529046
1201	4	0.3546105007654461
1201	5	0.29959720406927914
1201	6	0.22641138991363619
1201	7	0.2508075107133223
1201	8	0.1963631746545289
1201	9	0.3511486522243601
1201	10	0.7573424788302958
1201	11	0.392107317144486
1201	12	0.36352412808102486
1201	13	0.3014577868252175
1201	14	0.8697443681927055
1201	15	0.575526295397367
1201	16	0.4192071068124008
1201	17	0.42655241867591087
1201	18	0.34449017368077506
1201	19	0.7020960047590634
1201	20	0.8617981078533816
1201	21	0.7100181647705597
1201	22	0.765177049937023
1201	23	0.30569140140386253
1201	24	0.7405468846016205
1201	25	0.8963408181287917
1202	1	0.29755509251106815
1202	2	0.6274271199894557
1202	3	0.41139457323343365
1202	4	0.3212397050451017
1202	5	0.15471657712050302
1202	6	0.07294408953183762
1202	7	0.026458147796269316
1202	8	0.11777949061163895
1202	9	0.682705129906104
1202	10	-0.046463854941059424
1202	11	0.18006208024077353
1202	12	0.19329534068261722
1202	13	0.08992423130508342
1202	14	0.8485836988636493
1202	15	0.5024731043871427
1202	16	0.4253973761170684
1202	17	-1.5650522223906762
1202	18	-0.14191137604753123
1202	19	0.3792867073918913
1202	20	0.4998759114012046
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2210	18	0.6662717718683311
2210	19	0.9187275482110877
2210	20	1.0076839248277878
2210	21	1.0665492418637754
2210	22	1.1493020776877003
2210	23	1.5037491468108612
2210	24	1.306418832482727
2210	25	1.2160132215121067
2211	1	0.1563828586941085
2211	2	-0.45400220997600726
2211	3	0.04949200478445093
2211	4	0.34427283324644975
2211	5	0.2660699122181782
2211	6	0.30591257938299776
2211	7	0.2765042941177924
2211	8	0.3421273283507915
2211	9	0.7369647452585042
2211	10	0.843986955257698
2211	11	0.45784903331055204
2211	12	0.3120170959513402
2211	13	0.27920588969165294
2211	14	-0.8988960922300251
2211	15	0.06983299879497906
2211	16	0.28844003508319105
2211	17	0.43691732941212535
2211	18	0.31414283708650004
2211	19	0.6135741780374673
2211	20	0.8122236955402684
2211	21	0.8404827172646563
2211	22	0.1686554441286532
2211	23	0.6562616130684091
2211	24	0.15486807924760626
2211	25	0.47286639707807154
2212	1	-0.024146939020731395
2212	2	-1.1645772135529597
2212	3	-0.36587185641617737
2212	4	0.14788146698698768
2212	5	0.1508750194245394
2212	6	0.07377843345697954
2212	7	0.12920216770489645
2212	8	0.2932569639104585
2212	9	-2.0520027419496287
2212	10	0.042400832469446925
2212	11	0.07917822873478286
2212	12	0.02351733782143839
2212	13	0.20028912707181945
2212	14	0.745858392586662
2212	15	0.28879459254859796
2212	16	0.278128363317947
2212	17	0.44710261553423436
2212	18	0.23191296510731974
2212	19	-0.5171279947054757
2212	20	0.33497481461530043
2212	21	0.26204509590377967
2212	22	-1.6181384484810266
2212	23	-0.3367951821187205
2212	24	0.2939157923828617
2212	25	-0.2708081888471341
2213	1	0.2339363384004507
2213	2	0.46246710382573397
2213	3	0.21019051000208933
2213	4	0.3272520567117141
2213	5	0.36368501216946214
2213	6	0.28360258938240435
2213	7	0.2529012190880948
2213	8	0.2174219688423804
2213	9	0.768839120295894
2213	10	0.6918063164924959
2213	11	0.16585581922934267
2213	12	0.16384412339414212
2213	13	0.12776708688497962
2213	14	-1.6435314559262153
2213	15	-0.32239396198514214
2213	16	0.04438903813429107
2213	17	-2.16394608046933
2213	18	-0.8204676990352766
2213	19	-0.3758978193643969
2213	20	0.22647839825036442
2213	21	-0.1711185117216445
2213	22	0.36706323169691757
2213	23	0.8736445474227637
2213	24	0.6945216897153585
2213	25	-0.8351386795412736
2214	1	0.5159395797954787
2214	2	0.3688439101146841
2214	3	0.29061563042134964
2214	4	0.7468529964460799
2214	5	0.5906416263715499
2214	6	0.6054209082758817
2214	7	0.5398266046846771
2214	8	0.47169585582760476
2214	9	0.8976720655595543
2214	10	0.9656650626156136
2214	11	0.2900447405263762
2214	12	0.18533634173639513
2214	13	0.30046920669109056
2214	14	1.1723080187568655
2214	15	0.8065278405582035
2214	16	0.8186238697521446
2214	17	0.7668199355214895
2214	18	0.6060036646472469
2214	19	-0.47619101788219353
2214	20	0.43951488115808246
2214	21	0.8075858541385088
2214	22	0.95126628997361
2214	23	1.5199940123398221
2214	24	1.2795696983579177
2214	25	1.3784193986487736
2215	1	0.012285579411102532
2215	2	0.47005374699294933
2215	3	0.3201482140872294
2215	4	0.5918178796048394
2215	5	0.5470170299050423
2215	6	0.5599807996343813
2215	7	0.5702951238684477
2215	8	0.4339846148433324
2215	9	0.6879741247258124
2215	10	0.9737572219222486
2215	11	0.6122509155653688
2215	12	0.6014393522185983
2215	13	0.3580736388492127
2215	14	1.2630515962119162
2215	15	0.7533468356297135
2215	16	0.7726046298912621
2215	17	0.9024526022524881
2215	18	0.8175750983544958
2215	19	0.005371119991856688
2215	20	-1.320202967733671
2215	21	-1.60997640483275
2215	22	0.05299024940928376
2215	23	0.8917363823366955
2215	24	1.0677485016417023
2215	25	0.33400212899635306
2216	1	0.3368782039466147
2216	2	0.1460933669252995
2216	3	0.24687202202748182
2216	4	0.7165355020844615
2216	5	0.6360195765015888
2216	6	0.5110743851986044
2216	7	0.6416683641697816
2216	8	0.5147178760347373
2216	9	0.8642860229845155
2216	10	-1.0356915231754513
2216	11	0.08588768208394981
2216	12	0.38621938805525247
2216	13	0.5550909013176337
2216	14	0.18141419524090452
2216	15	0.14445265062509094
2216	16	0.5749005546383543
2216	17	0.9761550219810786
2216	18	0.848179317418122
2216	19	-0.594612342789155
2216	20	-0.3183330453527091
2216	21	-0.215312203878959
2216	22	-0.13319252110660784
2216	23	-4.000875738994225
2216	24	-1.5477074896417804
2216	25	-0.3949705604849143
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	17.0
4	30.0
5	68.0
6	79.0
7	268.0
8	155.0
9	531.0
10	1540.0
11	3335.0
12	6515.0
13	10454.0
14	14873.0
15	18927.0
16	21659.0
17	23706.0
18	25016.0
19	25409.0
20	25526.0
21	26373.0
22	27282.0
23	28048.0
24	29832.0
25	32439.0
26	36262.0
27	40913.0
28	47568.0
29	57412.0
30	68856.0
31	84604.0
32	108508.0
33	142611.0
34	189930.0
35	256454.0
36	354944.0
37	519811.0
38	829789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.931047115024505	20.937950253573	13.866657072159958	23.264345559242535
2	15.999882319675333	21.131790522484298	39.8749319660623	22.993395191778067
3	18.046371093636342	26.145963117721926	30.436167086063335	25.3714987025784
4	12.862733814831403	17.767442316683614	37.653432553759835	31.716391314725144
5	13.381648814854852	37.520195485432275	35.36496401680317	13.733191682909707
6	30.457639318839906	38.339837352662556	17.571149220661795	13.631374107835747
7	25.894238020844064	32.8793942859123	23.693617622223726	17.53275007101992
8	24.20116449268326	36.52573290818136	21.013832478223385	18.259270120911992
9	24.82292945818245	17.422878194037406	21.798900697950874	35.95529164982928
10	15.434758445736104	29.815458949832298	33.49967635847712	21.25010624595448
11	31.752101854025184	25.722711530628324	23.734523365084804	18.79066325026169
12	21.223175972680004	27.501813411629566	31.021940401111287	20.253070214579143
13	26.344988203248693	24.232786174606304	26.825325370975712	22.5969002511693
14	21.49457756678731	23.503738650816224	27.75295152341438	27.248732258982077
15	22.933202382614333	30.766444226992434	24.26028809376141	22.040065296631823
16	22.37902626032667	30.8794948853348	25.53913125063543	21.2023476037031
17	20.70132814024732	30.1224051868965	27.978001522750205	21.198265150105968
18	20.723941871762715	29.77614116484008	28.872938305765615	20.626978657631586
19	21.367596804046133	28.698598746436065	29.293541471757546	20.64026297776026
20	22.546350080314642	28.185111358523653	28.620614810713064	20.647923750448644
21	22.97313243154812	28.565582303889826	27.626457872108972	20.834827392453075
22	22.309333267084774	27.998559093824305	28.912919972829375	20.77918766626155
23	22.5086552640448	28.344547338925135	28.191529527599595	20.955267869430465
24	22.02141684326622	27.814797489916423	28.512271748549693	21.651513918267668
25	22.680076767494345	27.906018055934528	28.049580860735574	21.364324315835553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3100.0
1	3100.0
2	3306.0
3	3512.0
4	3512.0
5	3512.0
6	4843.0
7	6174.0
8	6174.0
9	6174.0
10	9076.5
11	11979.0
12	11979.0
13	11979.0
14	19287.0
15	26595.0
16	26595.0
17	26595.0
18	41770.0
19	56945.0
20	56945.0
21	56945.0
22	81917.5
23	106890.0
24	106890.0
25	106890.0
26	139615.5
27	172341.0
28	172341.0
29	172341.0
30	208501.0
31	244661.0
32	244661.0
33	244661.0
34	277490.5
35	310320.0
36	310320.0
37	310320.0
38	335499.0
39	360678.0
40	360678.0
41	360678.0
42	366611.0
43	372544.0
44	372544.0
45	372544.0
46	362758.5
47	352973.0
48	352973.0
49	352973.0
50	338546.5
51	324120.0
52	324120.0
53	324120.0
54	294230.5
55	264341.0
56	264341.0
57	264341.0
58	228215.5
59	192090.0
60	192090.0
61	192090.0
62	159106.0
63	126122.0
64	126122.0
65	126122.0
66	97582.5
67	69043.0
68	69043.0
69	69043.0
70	51026.5
71	33010.0
72	33010.0
73	33010.0
74	23485.0
75	13960.0
76	13960.0
77	13960.0
78	9779.0
79	5598.0
80	5598.0
81	5598.0
82	3781.5
83	1965.0
84	1965.0
85	1965.0
86	1281.0
87	597.0
88	597.0
89	597.0
90	368.5
91	140.0
92	140.0
93	140.0
94	87.5
95	35.0
96	35.0
97	35.0
98	23.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07974520752546371
2	0.019936301881365928
3	0.03222490763119149
4	0.040362840694240856
5	0.049415882696107034
6	0.01996898434346653
7	0.024315751802846314
8	0.03510096429604428
9	0.03519901168234608
10	0.02630938199098291
11	0.02545963797636731
12	0.01941338248775633
13	0.0278454577097111
14	0.03774824372619287
15	0.03555851876545267
16	0.02601523983207751
17	0.02634206445308351
18	0.027878140171811703
19	0.020491903737076128
20	0.01722365752701614
21	0.013269079612843553
22	0.018269496314235335
23	0.020393856350774328
24	0.018432908624738335
25	0.10598922459224543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3059745.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.06024296886852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.58472958545285	68.36885271803096
2	8.746472950217333	13.655036072292026
3	1.8337470790064732	4.2942822759209465
4	0.674492785369895	2.1060428282689156
5	0.32608402729415403	1.2727099199424408
6	0.1977017621170321	0.9259588551737384
7	0.12133275887065752	0.6629885246268664
8	0.08904949959789661	0.5560980459894376
9	0.06914172383978297	0.485749778599788
>10	0.3311474218803195	4.43706893910034
>50	0.017791017010544866	0.9738896626830588
>100	0.007090767953091403	0.9114533159990587
>500	7.143642639278016E-4	0.3821764308067207
>1k	5.042571261311002E-4	0.9676926325656733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4950	0.16177818739796943	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4568	0.14929348687554028	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3770	0.12321288211926157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.268246210059989E-5	0.0
3	3.268246210059989E-5	0.0	0.0	3.268246210059989E-5	0.0
4	3.268246210059989E-5	0.0	0.0	6.536492420119978E-5	0.0
5	3.268246210059989E-5	0.0	0.0	6.536492420119978E-5	0.0
6	3.268246210059989E-5	0.0	0.0	6.536492420119978E-5	0.0
7	3.268246210059989E-5	0.0	0.0	9.804738630179966E-5	0.0
8	3.268246210059989E-5	0.0	0.0	9.804738630179966E-5	0.0
9	6.536492420119978E-5	0.0	0.0	1.6341231050299944E-4	0.0
10	6.536492420119978E-5	0.0	0.0	1.9609477260359933E-4	0.0
11	6.536492420119978E-5	0.0	0.0	1.9609477260359933E-4	0.0
12	6.536492420119978E-5	0.0	0.0	2.287772347041992E-4	0.0
13	6.536492420119978E-5	3.268246210059989E-5	0.0	2.287772347041992E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCCG	50	0.0014981823	13.302289	5
TGGTATC	1390	0.0	11.482864	2
GTGGTAT	1460	0.0	11.200119	1
GGTATCA	3175	0.0	10.86951	1
GGGTCGC	85	6.604245E-4	10.057595	13
GTCTTAG	235	0.0	9.709355	1
TATACCG	80	0.004511149	9.501636	5
AGTACCG	80	0.004511149	9.501636	5
AGCGCAA	90	0.0011152416	9.498995	10
TATACTG	720	0.0	9.237701	5
TAGTACA	325	0.0	9.063098	4
GGTGTCG	105	4.5008402E-4	9.046366	8
CTATACT	485	0.0	8.81595	4
GTGTTAG	390	0.0	8.7757635	1
CGGATAG	130	4.3623222E-5	8.7707405	5
TAGGACC	130	4.3623222E-5	8.7707405	4
TAGTACC	195	1.6456397E-8	8.7707405	4
GTATTAT	540	0.0	8.626793	1
TAGACAG	335	0.0	8.508927	5
TCTATAC	370	0.0	8.474431	3
>>END_MODULE