FastQCFastQC Report
Wed 25 May 2016
SRR1295267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059745
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125540.41029562921093093No Hit
GGTATCAACGCAGAGTACTTTTTTT109740.35865733909198316No Hit
TATCAACGCAGAGTACTTTTTTTTT77000.25165495817461914No Hit
GTGGTATCAACGCAGAGTACATGGG57150.18678027090492835No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53680.1754394565560202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.012.1581321
GGACCGT550.003077911712.0865526
ATCGCTA550.003077911712.08655210
TCGCTAT1001.939794E-611.39589211
AGTACCG600.00584579311.0914835
GTGGTAT14150.010.8966231
AGCGTCA2950.010.3126633
TGGTATC14400.010.2992332
GTACTAG1855.820766E-1110.2894371
TAGGACT4200.09.5069864
TGTCCCG1002.7365214E-49.5069855
CCGTCTG900.00111784199.4965769
TCTGTCG1304.261239E-69.4965768
CTACACT5000.09.3168464
TACACTG6350.09.2824125
AGGACTG5150.09.23008255
GTAGGAC2501.8189894E-129.1267063
CCTACAC3650.09.1162883
CCGGTCG1054.5108367E-49.0443589
CAGCGCA950.00183050338.9967579
Produced by FastQC (version 0.11.5)