FastQCFastQC Report
Wed 25 May 2016
SRR1295266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962239
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA524991.7722742830676392No Hit
GCTTATGTACTCTGCGTTGATACCA309431.044581480427474No Hit
GTATCAACGCAGAGTACTTTTTTTT220720.7451120588176713No Hit
CTTATGTACTCTGCGTTGATACCAC204600.6906937623871673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA144660.48834682144148395No Hit
GAGTACATAAGCAGTGGTATCAACG143220.4834856336710171No Hit
GGTATCAACGCAGAGTACTTTTTTT142940.4825404027156486No Hit
TATCAACGCAGAGTACTTTTTTTTT130960.44209802112523666No Hit
TATGTACTCTGCGTTGATACCACTG129270.4363928771446193No Hit
GTGGTATCAACGCAGAGTACATGGG120120.4055040798531111No Hit
GTACTGGTTCACTATCGGTCAGTCA115240.3890300546309734No Hit
GCGTTGATACCACTGCTTATGTACT107240.3620234559061575No Hit
CATAAGCAGTGGTATCAACGCAGAG89610.3025076639663444No Hit
CCCATGTACTCTGCGTTGATACCAC82140.2772902524070475No Hit
CTGCTTATGTACTCTGCGTTGATAC80070.2703022949870014No Hit
ACTCTGCGTTGATACCACTGCTTAT76900.2596009302422931No Hit
GGATACCACGTGTCCCGCCCTACTC76840.25939838075185695No Hit
ATACAGGGTGACAGCCCCGTACACA76210.2572716111022777No Hit
ACGCAGAGTACATAAGCAGTGGTAT76110.2569340286182175No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71980.24299187202653127No Hit
GTATCAACGCAGAGTACATAAGCAG68940.2327293645111012No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63790.215343866582001No Hit
CATGTACTCTGCGTTGATACCACTG58520.19755326967202852No Hit
TATCAACGCAGAGTACATAAGCAGT57280.19336724686968204No Hit
ACCCTGTATCGCGCGCCTTTCCAGA55120.18607546521398172No Hit
GGTATCAACGCAGAGTACATAAGCA54840.18513023425861316No Hit
GTACATGATAAGCAGTGGTATCAAC52160.17608302368579984No Hit
ATCATGTACTCTGCGTTGATACCAC50950.17199827562867143No Hit
ATCATTAACTGAATCCATAGGTTAA50140.1692638575077838No Hit
CCCATATTCAGACAGGATACCACGT44440.15002165591635247No Hit
GGTATCAACGCAGAGTACATGGGGG43400.1465107980821264No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42570.14370886346442674No Hit
GTGGTATCAACGCAGAGTACATAAG41440.13989418139454649No Hit
AAGCAGTGGTATCAACGCAGAGTAC41330.13952284066208026No Hit
GTATTTAGCCTTGGAGGATGGTCCC40060.13523554311451574No Hit
GGTTAATGAGGCGAACCGGGGGAAC40030.13513426836929768No Hit
GTTCACTATCGGTCAGTCAGGAGTA39890.13466165289161341No Hit
TCTAAGTACCCCGAGGAAAAGAAAT37210.12561444231880006No Hit
GCCTTGGAGGATGGTCCCCCCATAT36310.12257619996225827No Hit
CTATCGGTCAGTCAGGAGTATTTAG35970.1214284195164536No Hit
TATTCAGACAGGATACCACGTGTCC35530.11994305658658871No Hit
GCCCAGAGCCTGAATCAGTGTGTGT34640.11693857247845295No Hit
GTCAGGAGTATTTAGCCTTGGAGGA34280.11572327553583625No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG33000.1114022197398657No Hit
ACATAAGCAGTGGTATCAACGCAGA32670.11028819754246703No Hit
GTATCAACGCAGAGTACATGGGGGT32050.1081951861412938No Hit
GTACATGGGAAGCAGTGGTATCAAC31090.10495439429431588No Hit
GGTATCAACGCAGAGTACATGGGCA31030.10475184480387975No Hit
GTTAATGATAGTGTGTCGAAACACA30640.103435273116045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG350.00217300316.28326812
ATCCGTT456.769993E-414.7763087
GTACAAA5000.014.259661
CCTAGTT954.9021764E-914.0012072
GATCGTA1650.013.81610711
AATCGCC2200.012.95216118
ATCGCCG2000.012.83738119
CCCCTAG751.4693804E-512.6752531
TCGTTGA1501.8189894E-1212.66497914
CTAGTTC1155.324182E-912.3923733
GACCGAA550.003054035412.0991041
CTAGTAG550.003067592412.0919513
TCTAAGG1103.814057E-812.0919512
GACAGCG951.0420699E-611.9988067
ATCGTAA1750.011.94106412
GACAACG802.8402274E-511.88646519
GGATCGT2000.011.87361710
GATCGAA802.8768025E-511.87161215
CTAGGAC1052.7198257E-711.7629183
GTACGAT657.9816836E-411.7002331
Produced by FastQC (version 0.11.5)