##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295266_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2962239
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.35984773679639	31.0	29.0	33.0	23.0	34.0
2	28.999878807888223	31.0	28.0	33.0	18.0	34.0
3	29.30844472711351	31.0	28.0	34.0	22.0	34.0
4	32.12516073146022	35.0	32.0	37.0	18.0	37.0
5	32.90577498979657	35.0	33.0	37.0	25.0	37.0
6	33.02803555013623	35.0	33.0	37.0	25.0	37.0
7	32.9758797315139	35.0	33.0	37.0	24.0	37.0
8	32.91637778045593	35.0	33.0	37.0	23.0	37.0
9	33.523321041955086	37.0	32.0	39.0	17.0	39.0
10	33.506604632509394	37.0	32.0	39.0	17.0	39.0
11	33.962696797928864	37.0	33.0	39.0	21.0	39.0
12	33.971953647224275	37.0	33.0	39.0	21.0	39.0
13	33.74011921387842	37.0	33.0	39.0	18.0	39.0
14	33.80675428282458	38.0	32.0	40.0	17.0	41.0
15	34.409199257723635	38.0	32.0	40.0	17.0	41.0
16	34.50049776537275	38.0	32.0	40.0	18.0	41.0
17	34.46519271402477	38.0	32.0	40.0	18.0	41.0
18	34.45340872225368	38.0	32.0	40.0	18.0	41.0
19	34.53568533801628	39.0	32.0	40.0	18.0	41.0
20	34.56627841305175	39.0	32.0	40.0	18.0	41.0
21	34.58139603185293	39.0	32.0	40.0	19.0	41.0
22	34.36293560377809	38.0	32.0	40.0	18.0	41.0
23	34.08607880728057	38.0	31.0	40.0	18.0	41.0
24	34.43567787744338	38.0	32.0	40.0	21.0	41.0
25	33.2027108548635	37.0	30.0	40.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4698963950825039
1101	2	0.685650126969847
1101	3	0.4909458374673292
1101	4	0.3453434144409613
1101	5	0.41577355620405854
1101	6	0.19366239633927762
1101	7	0.22444252468319092
1101	8	0.3671279037502728
1101	9	0.3183593533953868
1101	10	0.5335108581383565
1101	11	0.42316040410521794
1101	12	0.3163266990505704
1101	13	0.4803804989229832
1101	14	1.0298279978147988
1101	15	0.772420652202392
1101	16	0.5197339664412652
1101	17	0.7898987431455495
1101	18	0.8389891296961238
1101	19	0.762833162269466
1101	20	0.8607062613197485
1101	21	0.7735456639772664
1101	22	0.7944303973947413
1101	23	-1.425086697963799
1101	24	0.11486615396190558
1101	25	0.5002080233907833
1102	1	0.5100903049162504
1102	2	0.46118066352711296
1102	3	0.4204906482992783
1102	4	0.35805388570325647
1102	5	0.3258534922218246
1102	6	0.12118638072481502
1102	7	0.07312772447649962
1102	8	0.13280676220610133
1102	9	0.47271180780447253
1102	10	0.7956085177587795
1102	11	0.607379718671119
1102	12	0.47614324142447373
1102	13	0.5060099386249846
1102	14	1.1397285854854502
1102	15	0.7209765966742196
1102	16	0.5258734698987482
1102	17	0.8821005409650482
1102	18	0.82945932190934
1102	19	0.718307901342186
1102	20	0.7942859624363336
1102	21	0.8612482886250632
1102	22	0.6722896380811392
1102	23	0.8306514745328357
1102	24	0.7562119249250969
1102	25	0.5620671419524328
1103	1	-0.3145997914626797
1103	2	-1.4723246888946449
1103	3	-0.42503274635284427
1103	4	-0.1871015457300409
1103	5	-0.31254270815615115
1103	6	-0.31228889009376104
1103	7	-0.3333801253494073
1103	8	-0.045916456752628676
1103	9	0.512241979442102
1103	10	0.5739123613041528
1103	11	0.35973660176264843
1103	12	-0.030226485459813546
1103	13	-0.10357397600552787
1103	14	-1.1311646656332428
1103	15	-0.4019835340524338
1103	16	-0.2113414280257473
1103	17	0.18312525079327457
1103	18	0.40280626418800125
1103	19	0.35155699216254277
1103	20	0.21148014402319149
1103	21	0.2949991321721157
1103	22	0.005603477974645443
1103	23	0.7657126558857783
1103	24	0.5876232611877796
1103	25	0.47076611051105743
1104	1	0.03876871002042037
1104	2	0.12170798663133908
1104	3	-0.24558404963991975
1104	4	-0.19955367392669388
1104	5	-0.14915302448788026
1104	6	-0.07722209280685632
1104	7	-0.13522880450600638
1104	8	-0.12067798149807629
1104	9	-2.086333179694549
1104	10	-0.6971422594153864
1104	11	-0.3754146258938391
1104	12	0.038451620350791416
1104	13	0.15259165713683842
1104	14	-1.6269367689310457
1104	15	-0.6155677751293567
1104	16	-0.1597277161013011
1104	17	0.17186757989961166
1104	18	0.31063127300399884
1104	19	0.3360432090398433
1104	20	0.3389998932703975
1104	21	0.5209057748854917
1104	22	0.5468903611275522
1104	23	0.9716728670200752
1104	24	0.6426377008349817
1104	25	0.4723942869469582
1105	1	-0.14208247555792042
1105	2	0.011995806080015825
1105	3	-1.3528549584137473
1105	4	-0.5417364752738543
1105	5	-0.45094714374381795
1105	6	-0.1459850799285931
1105	7	-0.11308049584122415
1105	8	-0.2295209153751685
1105	9	0.2868877860858632
1105	10	0.40199863634651223
1105	11	-0.11412032505049297
1105	12	-0.1533740800993897
1105	13	-0.12224017108766816
1105	14	0.618275470994476
1105	15	0.3218781200661027
1105	16	-0.11088049426260227
1105	17	0.33240990204920706
1105	18	0.3111548788941292
1105	19	0.14600429925302905
1105	20	-0.5469662614412272
1105	21	0.0039753611318502635
1105	22	0.15002287247558144
1105	23	0.3125347893015089
1105	24	0.25833528362786495
1105	25	0.44857205634387043
1106	1	-1.4591367823235828
1106	2	-0.8775072724744959
1106	3	-0.9531182775651814
1106	4	-1.3637474520269137
1106	5	-0.9018354388153824
1106	6	-0.6823901795412937
1106	7	-0.5214985444633058
1106	8	-0.7684099112857226
1106	9	-0.3610229523452517
1106	10	-1.7873759244824576
1106	11	-1.276717493073889
1106	12	-1.0052106176612412
1106	13	-1.0883401809948197
1106	14	-0.2744702492093225
1106	15	-0.8484594660022466
1106	16	-1.428470291551342
1106	17	-0.6235504170710371
1106	18	-0.7841300983836419
1106	19	-0.7166447895645476
1106	20	-0.31046039329068975
1106	21	-0.3298905960729428
1106	22	-0.3091791709654288
1106	23	-0.6470028817114581
1106	24	-0.8265753960942206
1106	25	-1.6393880527081421
1107	1	0.18891331279372636
1107	2	0.3005021335707383
1107	3	0.06480340918180616
1107	4	0.23068554311153378
1107	5	0.011639513625965492
1107	6	0.05554049050731891
1107	7	0.04233574004314278
1107	8	-0.04491857752964279
1107	9	0.6454238876462952
1107	10	0.07807799646448643
1107	11	0.15369362508294415
1107	12	0.03158921324131825
1107	13	0.13714597415208374
1107	14	-0.9649347387464147
1107	15	-0.34039827386527577
1107	16	-0.08117585062976218
1107	17	0.1721481667378839
1107	18	0.5037688658945214
1107	19	0.3282135913274402
1107	20	0.527451781725162
1107	21	0.5546422722424253
1107	22	-0.1244428572896723
1107	23	0.6709690058387423
1107	24	0.5472065738909819
1107	25	0.254498017890036
1108	1	-0.10523177723735344
1108	2	0.20665404335985116
1108	3	-0.11443820693755669
1108	4	-0.44393003132969255
1108	5	-0.4299675237331755
1108	6	-0.34845834259299835
1108	7	-0.21739759769358358
1108	8	-0.22037180613556728
1108	9	0.2329042099748122
1108	10	0.23053986122889114
1108	11	-0.06683225036702822
1108	12	-0.14915483208429947
1108	13	-0.12781543192533462
1108	14	0.00494599346478708
1108	15	0.004141378706854937
1108	16	-0.14125297425874805
1108	17	0.23397563988634573
1108	18	0.26047679604199914
1108	19	0.2659598603675377
1108	20	0.351063657314576
1108	21	0.27039115212881626
1108	22	0.2402837927908763
1108	23	0.0025915743390498847
1108	24	0.10291121800492675
1108	25	-0.6644692391985103
1109	1	-0.7483870073156709
1109	2	-0.7136083197103815
1109	3	-0.797393963124069
1109	4	-0.7591737097593487
1109	5	-0.872088906183528
1109	6	-1.1886392680120963
1109	7	-0.8940347477352049
1109	8	-1.240358378315932
1109	9	-0.9294618985641421
1109	10	-1.109278459334
1109	11	-1.2299551558596704
1109	12	-1.1927192883048434
1109	13	-1.2506352870167845
1109	14	-0.6755133054022053
1109	15	-1.4192879526681281
1109	16	-1.5044791542830538
1109	17	-1.4128931289282036
1109	18	-2.039578375104341
1109	19	-2.171620138257815
1109	20	-1.9449483006330794
1109	21	-1.6093145362979726
1109	22	-1.7949490885454011
1109	23	-1.0452511004500096
1109	24	-2.1853363922488995
1109	25	-1.2310589879993543
1110	1	0.0468949139346968
1110	2	0.17129026108570855
1110	3	-0.06691752265837181
1110	4	0.17053297120062894
1110	5	0.06458998915115188
1110	6	0.2026764244940935
1110	7	0.18479864762316822
1110	8	0.2938168056192296
1110	9	0.41242109343554745
1110	10	0.3443135251451679
1110	11	0.2606191200730166
1110	12	0.14977868838084163
1110	13	0.17988420972442754
1110	14	-2.4639879633674866
1110	15	-0.5648353386342109
1110	16	-0.11670862439329
1110	17	-0.10749729958241971
1110	18	-2.7866796995256635
1110	19	-0.5105968316607701
1110	20	-2.064550790549575
1110	21	-0.39436802049480946
1110	22	-1.957793745397538
1110	23	-0.6251270124109496
1110	24	0.07644273199674245
1110	25	0.030435788488247795
1111	1	-0.10676237423725965
1111	2	-1.2382368421484422
1111	3	-0.20773754597576755
1111	4	0.15990755952282854
1111	5	0.11733432645979747
1111	6	0.07499839182037249
1111	7	0.19988887945142864
1111	8	0.10352660612921483
1111	9	-1.3687341386146912
1111	10	-0.13317572091934693
1111	11	0.0839452746149405
1111	12	0.04187129599922912
1111	13	0.26557391353406956
1111	14	1.04898663538863
1111	15	0.4643626261982945
1111	16	0.41882570208584724
1111	17	0.12388821291747121
1111	18	0.2860801470127683
1111	19	0.1397234154504261
1111	20	0.5683666600287935
1111	21	-0.34667817537808787
1111	22	-0.008025995163507105
1111	23	0.17273389546806328
1111	24	-0.2506063805066745
1111	25	-1.6278916296983184
1112	1	0.1687174526320625
1112	2	0.3732659054471732
1112	3	0.07335737550357635
1112	4	0.3405520955242167
1112	5	0.16945122946455626
1112	6	0.09369651660421141
1112	7	0.07655587650724982
1112	8	0.00641336233218226
1112	9	-0.2710999615384466
1112	10	0.53411530519584
1112	11	0.20387835348947903
1112	12	-0.021479894403022115
1112	13	0.19390764781280012
1112	14	0.6147143039760365
1112	15	0.2539785881009564
1112	16	0.2270017182162718
1112	17	-3.8526901959064013
1112	18	-0.9513482711355152
1112	19	-1.0323015126223325
1112	20	0.10405494132837134
1112	21	-0.180619897942627
1112	22	-1.5788118709681171
1112	23	-1.2307072976711027
1112	24	-0.040868967213754104
1112	25	0.41465007179229474
1113	1	-0.35264163589875963
1113	2	-0.20065642319854504
1113	3	-0.11099516744845062
1113	4	-0.30745166311157135
1113	5	-0.00478716628135345
1113	6	-0.06943468327619229
1113	7	-0.27615151583239594
1113	8	-0.1812600207603623
1113	9	0.197484463614515
1113	10	0.3913682976892474
1113	11	0.08879789519217951
1113	12	-0.05580734419000066
1113	13	-0.3034986799359629
1113	14	-1.2129031266974266
1113	15	-1.7160313423856337
1113	16	-0.37556098800149584
1113	17	-0.10923630860141031
1113	18	-0.5802513260948814
1113	19	-0.07403731896716437
1113	20	0.4187915698747844
1113	21	-1.3724555430505276
1113	22	-1.8500593749990415
1113	23	-0.6263870021056519
1113	24	-2.3555201722195775
1113	25	-0.42759400461906694
1114	1	0.024293558675921645
1114	2	0.1286713219738438
1114	3	-0.021099491072945398
1114	4	0.30006978045386035
1114	5	0.07715902227412386
1114	6	0.24623791307608656
1114	7	0.2812792923395193
1114	8	0.18890169846899596
1114	9	0.47112368240066615
1114	10	0.4397068628691869
1114	11	0.29573545806044876
1114	12	0.40164165917095573
1114	13	0.25519843555250077
1114	14	1.1312263171855932
1114	15	0.5335810417076061
1114	16	0.5044390353506216
1114	17	-0.3661570871375446
1114	18	0.08238387048418616
1114	19	-0.31596063023469867
1114	20	-1.5261004372997675
1114	21	-0.6505493333146788
1114	22	-1.352546939511683
1114	23	-2.6845155130717053
1114	24	-1.6791261045128465
1114	25	-1.1788920416208768
1115	1	0.22661398289311308
1115	2	0.2643208778538231
1115	3	0.28467501628979264
1115	4	0.5202004661837165
1115	5	0.36234224438373275
1115	6	0.29970692592803516
1115	7	0.45393041213637986
1115	8	0.4605448650545583
1115	9	0.5726546402747914
1115	10	0.9436896677046889
1115	11	0.49827733097920657
1115	12	0.5195718749336251
1115	13	0.321203235788893
1115	14	1.0292369639432337
1115	15	0.5540342228715858
1115	16	0.5159498906419842
1115	17	0.5288349004144877
1115	18	0.8811152414996144
1115	19	0.19424099140348972
1115	20	0.8521587417144758
1115	21	-0.3037410359107966
1115	22	0.9169705950582454
1115	23	1.0709372737551632
1115	24	0.9352345854841175
1115	25	1.1461474695801925
1116	1	0.36312376870879604
1116	2	0.6125117925496468
1116	3	0.5786008944680567
1116	4	0.8923604466625505
1116	5	0.6361882450049734
1116	6	0.6222972700967446
1116	7	0.59730814296055
1116	8	0.48337652821224
1116	9	0.7817971241082375
1116	10	0.7761990012235813
1116	11	0.5344235397572703
1116	12	0.5223885430264517
1116	13	-0.12302587400763798
1116	14	1.0201286923031176
1116	15	0.7316353917594611
1116	16	0.8159056874851842
1116	17	0.8947865745551837
1116	18	0.976666700368348
1116	19	1.1615969091726157
1116	20	1.0712470141883443
1116	21	0.6141685457914647
1116	22	0.5608345919514122
1116	23	-0.2939788272270718
1116	24	0.15587043860895733
1116	25	-0.3734897887051005
1201	1	0.36309017115857856
1201	2	0.6302677834240491
1201	3	0.4172743068760063
1201	4	0.2670379461136676
1201	5	0.27640775558748487
1201	6	0.28411789439312685
1201	7	0.19408305662202707
1201	8	0.1418176342220363
1201	9	0.3934694969684571
1201	10	0.9403433812158255
1201	11	0.5509406883421661
1201	12	0.4947998338155841
1201	13	0.44786154329656824
1201	14	1.0235084351722605
1201	15	0.6577868085519825
1201	16	0.4271767762362586
1201	17	0.5628093605215412
1201	18	0.4241378289334179
1201	19	0.7886804064512134
1201	20	0.7579890256071948
1201	21	0.6826675276239911
1201	22	0.8433992420823344
1201	23	0.43141505464106444
1201	24	0.807034935089483
1201	25	0.9571502158799774
1202	1	0.423964606281686
1202	2	0.784736448170797
1202	3	0.467303409181806
1202	4	0.06536663142554033
1202	5	0.044177426204015546
1202	6	0.05263534629084887
1202	7	-0.0021513250772855486
1202	8	0.0942451156014883
1202	9	0.6051934386424307
1202	10	-0.10780663035679794
1202	11	0.19475517726849745
1202	12	0.13580122632487246
1202	13	0.14844169226091708
1202	14	0.8151046605994097
1202	15	0.5485186158165547
1202	16	0.3439854636729436
1202	17	-1.4916334270771614
1202	18	-0.14490642402519427
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2210	14	-1.432333778799375
2210	15	-0.087530845861302
2210	16	0.11407820431892901
2210	17	0.6259396816887843
2210	18	0.5786916287765678
2210	19	0.82235460423707
2210	20	0.9322418622724413
2210	21	1.047648175365012
2210	22	1.118273406640597
2210	23	1.5820539114991163
2210	24	1.1517732184899003
2210	25	1.1692723751668765
2211	1	0.0370931373551322
2211	2	-0.6189605857275069
2211	3	-0.08248825748486155
2211	4	0.3950276483746933
2211	5	0.30838293467859756
2211	6	0.16588040278802652
2211	7	0.24475898565717813
2211	8	0.352285369838782
2211	9	0.8407089753655939
2211	10	0.9255231719030874
2211	11	0.6108427478899614
2211	12	0.4654269325395646
2211	13	0.37682799169594006
2211	14	-0.7935641247113239
2211	15	0.05869163841542502
2211	16	0.22629125463339506
2211	17	0.4045494825273579
2211	18	0.24076447992960937
2211	19	0.6570951702748076
2211	20	0.8315526589181417
2211	21	0.6542585003126007
2211	22	0.20401109007875107
2211	23	0.6340940058387403
2211	24	-0.0698986892669069
2211	25	0.47109889508302416
2212	1	0.019257376837330753
2212	2	-0.9984205371838186
2212	3	-0.21966261023566958
2212	4	0.1989596872096442
2212	5	0.18987363047147454
2212	6	0.27957174583009703
2212	7	0.14816311187077247
2212	8	0.23884483585819538
2212	9	-2.080549115249294
2212	10	0.1486643531328582
2212	11	0.24764695500970646
2212	12	0.20777725616415665
2212	13	0.2632701437995024
2212	14	0.6979144351592197
2212	15	0.42283201058371134
2212	16	0.429290850102646
2212	17	0.5663718935305937
2212	18	0.2708251595412605
2212	19	-0.45054641548571084
2212	20	0.5516214291446815
2212	21	0.6449846329986855
2212	22	-1.4423380199536098
2212	23	-0.1429063986920056
2212	24	0.49854791267483733
2212	25	-0.2500540175383392
2213	1	0.11662092628640153
2213	2	0.43641086746127655
2213	3	0.13946294876555854
2213	4	0.5312004383097815
2213	5	0.3542381428027994
2213	6	0.326571989149528
2213	7	0.28587610571339184
2213	8	0.31377256283884236
2213	9	0.678392589766986
2213	10	0.787614410275566
2213	11	0.1332015446509871
2213	12	0.1581127218723566
2213	13	0.2830900792954907
2213	14	-1.4633991227036987
2213	15	-0.2933654702642272
2213	16	-0.03453735240669431
2213	17	-2.1347107480153724
2213	18	-0.8042409573989033
2213	19	-0.4025182774933782
2213	20	0.3151634633081599
2213	21	-0.0604697587384635
2213	22	0.3586409753092923
2213	23	0.9458109050557653
2213	24	0.6276260714906385
2213	25	-0.6367913378019452
2214	1	0.16800980402179633
2214	2	0.07494774760582601
2214	3	0.20177007584847217
2214	4	0.6334943150413608
2214	5	0.5108746013452645
2214	6	0.5335720694546922
2214	7	0.5308339856571749
2214	8	0.40149370317211464
2214	9	0.8179923086989263
2214	10	0.7026815052364199
2214	11	0.0876094145566313
2214	12	0.04934359920622455
2214	13	0.16658632502927873
2214	14	1.024010875288674
2214	15	0.6887333050820885
2214	16	0.6068995879667298
2214	17	0.5924411491940234
2214	18	0.5590811465962773
2214	19	-0.6962881630585258
2214	20	0.378169325584814
2214	21	0.7377001669792662
2214	22	0.9323694234120836
2214	23	1.3774690058387407
2214	24	1.0973346440664216
2214	25	1.2916488950830214
2215	1	-0.06330434561106557
2215	2	0.2658642389731618
2215	3	0.2994473530860198
2215	4	0.5636966964662449
2215	5	0.5698153038656031
2215	6	0.4503332125386379
2215	7	0.49982608726463695
2215	8	0.3146826295463896
2215	9	0.41599631743081744
2215	10	0.7375465975166335
2215	11	0.5741650009825108
2215	12	0.33479726064698667
2215	13	0.17878692038549815
2215	14	1.0727755508195358
2215	15	0.5538958373305505
2215	16	0.5534940313086594
2215	17	0.7073114806089862
2215	18	0.659378705635767
2215	19	-0.03413134622979186
2215	20	-1.2130773106525368
2215	21	-1.4788038219074195
2215	22	0.20804979650198163
2215	23	0.8638172892283009
2215	24	0.9676839990971828
2215	25	0.286017777797845
2216	1	0.0021480676873935067
2216	2	-0.14534389226555788
2216	3	0.06474981861374474
2216	4	0.371895279671584
2216	5	0.39488681999478104
2216	6	0.2346994006444021
2216	7	0.26223687954784936
2216	8	0.0981689443296716
2216	9	0.388411279760021
2216	10	-1.482986340422741
2216	11	-0.31665457258163343
2216	12	-0.06681717249795582
2216	13	0.11953230573666929
2216	14	-0.2238836584733832
2216	15	-0.3179515823777166
2216	16	0.2824481410213906
2216	17	0.5513106025702328
2216	18	0.42913130736798166
2216	19	-1.024353114827015
2216	20	-0.6415354975663163
2216	21	-0.43010980086638995
2216	22	-0.4446591939512601
2216	23	-3.7750117015567604
2216	24	-1.575673072975377
2216	25	-0.8279404939845065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	41.0
5	61.0
6	98.0
7	378.0
8	339.0
9	824.0
10	1949.0
11	4322.0
12	8921.0
13	16089.0
14	24705.0
15	33807.0
16	40537.0
17	43886.0
18	43112.0
19	40592.0
20	37818.0
21	35225.0
22	32609.0
23	31240.0
24	30953.0
25	31883.0
26	34188.0
27	37707.0
28	43103.0
29	51580.0
30	62980.0
31	78180.0
32	102391.0
33	135524.0
34	180218.0
35	244944.0
36	340478.0
37	492113.0
38	699428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.31420636717153	19.59721203971796	12.733566001209512	20.355015591900997
2	15.142535699766752	23.379312300870733	39.44852926280134	22.02962273656118
3	19.14419266842582	31.539634630230097	28.60721432734695	20.708958373997138
4	12.536452305440516	20.59731292263145	33.52146409682205	33.344770675105984
5	11.178942312855966	43.77301124264172	30.85881731880879	14.189229125693526
6	27.71670479390189	38.014873842465576	19.800072596506926	14.4683487671256
7	23.49295719479095	39.32358409461273	19.348112520099868	17.835346190496452
8	23.555422865543626	37.45895324917349	21.076389932565633	17.909233952717248
9	26.572907931385142	21.586000088485623	18.66019933581746	33.180892644311776
10	16.09779125802965	31.795362212544266	27.55961854128952	24.54722798813657
11	28.852984567362416	30.67803586822811	19.820689249965643	20.64829031444383
12	23.466365387264688	31.647168144700554	24.79321932258056	20.093247145454203
13	27.00376788122698	26.403939289768907	26.655243932995333	19.937048896008776
14	24.60138297563345	27.971448855096924	24.420793674274584	23.00637449499504
15	26.879355617659723	29.324833880731855	23.954643318501507	19.84116718310692
16	22.022571771845595	30.84013023578729	24.981951092018026	22.155346900349095
17	19.289206574001756	32.7825845885576	25.293819896718574	22.63438894072208
18	19.16781202405757	28.55347271235264	31.54743130388386	20.73128395970593
19	21.655934172686283	31.32122285263173	26.829377825995493	20.19346514868649
20	23.076239979308635	28.366145308212865	28.640353157830408	19.917261554648093
21	24.499146448849057	29.86378971308796	25.308095500488903	20.32896833757408
22	22.676877299519703	28.591775485836852	25.756714192324942	22.97463302231851
23	24.12373856239165	26.749399460138807	26.399306838219257	22.727555139250285
24	21.69827152773393	28.1105257879661	25.74812165521927	24.443081029080705
25	22.954523257460473	29.49758180808637	26.43022423910006	21.117670695353105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17825.0
1	17825.0
2	15309.5
3	12794.0
4	12794.0
5	12794.0
6	18313.0
7	23832.0
8	23832.0
9	23832.0
10	25147.5
11	26463.0
12	26463.0
13	26463.0
14	31415.5
15	36368.0
16	36368.0
17	36368.0
18	48532.5
19	60697.0
20	60697.0
21	60697.0
22	77104.0
23	93511.0
24	93511.0
25	93511.0
26	117995.0
27	142479.0
28	142479.0
29	142479.0
30	162984.5
31	183490.0
32	183490.0
33	183490.0
34	195368.5
35	207247.0
36	207247.0
37	207247.0
38	226162.5
39	245078.0
40	245078.0
41	245078.0
42	365100.5
43	485123.0
44	485123.0
45	485123.0
46	444986.0
47	404849.0
48	404849.0
49	404849.0
50	375490.0
51	346131.0
52	346131.0
53	346131.0
54	295730.0
55	245329.0
56	245329.0
57	245329.0
58	211754.5
59	178180.0
60	178180.0
61	178180.0
62	154608.5
63	131037.0
64	131037.0
65	131037.0
66	97507.5
67	63978.0
68	63978.0
69	63978.0
70	50455.0
71	36932.0
72	36932.0
73	36932.0
74	25578.0
75	14224.0
76	14224.0
77	14224.0
78	9464.5
79	4705.0
80	4705.0
81	4705.0
82	2972.5
83	1240.0
84	1240.0
85	1240.0
86	851.5
87	463.0
88	463.0
89	463.0
90	325.5
91	188.0
92	188.0
93	188.0
94	120.0
95	52.0
96	52.0
97	52.0
98	38.0
99	24.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07997329047386116
2	0.020086157801581843
3	0.03257670971180921
4	0.03976721662229145
5	0.04921952617597702
6	0.02241547694159722
7	0.026230159011477468
8	0.0434131074501416
9	0.044088272418262
10	0.02936967611323732
11	0.026601499743943685
12	0.021841586718694878
13	0.030146115826575774
14	0.04648510805508941
15	0.042670425985209155
16	0.028390686909462743
17	0.027546730699312242
18	0.03406207264167409
19	0.02268554292884538
20	0.02082883926651428
21	0.016710332960979853
22	0.025352444552920947
23	0.024879829075236667
24	0.02437345534914637
25	0.1083639773833239
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2962239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.31742227931526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.53653418543782	20.13660022605923
2	6.953313702559615	3.5207995849650167
3	2.959910411954542	2.2481190542518537
4	1.7511767397795337	1.7734112402685207
5	1.2314023634986038	1.5587966816220509
6	0.9161346982705465	1.39165014125091
7	0.7064137178714296	1.251920207947644
8	0.5840734126040383	1.1829786583213737
9	0.45778476038086685	1.04309370824378
>10	4.11669257579606	21.54195323514315
>50	0.49389906124401217	8.588111137810179
>100	0.24941411977777603	11.213721822340245
>500	0.021153549469016764	3.7735702671669986
>1k	0.018189357823676835	9.05794675299203
>5k	0.00229051172594449	3.8941891605780357
>10k+	0.001616831806549052	7.823138121038946
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	52499	1.7722742830676392	No Hit
GCTTATGTACTCTGCGTTGATACCA	30943	1.044581480427474	No Hit
GTATCAACGCAGAGTACTTTTTTTT	22072	0.7451120588176713	No Hit
CTTATGTACTCTGCGTTGATACCAC	20460	0.6906937623871673	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	14466	0.48834682144148395	No Hit
GAGTACATAAGCAGTGGTATCAACG	14322	0.4834856336710171	No Hit
GGTATCAACGCAGAGTACTTTTTTT	14294	0.4825404027156486	No Hit
TATCAACGCAGAGTACTTTTTTTTT	13096	0.44209802112523666	No Hit
TATGTACTCTGCGTTGATACCACTG	12927	0.4363928771446193	No Hit
GTGGTATCAACGCAGAGTACATGGG	12012	0.4055040798531111	No Hit
GTACTGGTTCACTATCGGTCAGTCA	11524	0.3890300546309734	No Hit
GCGTTGATACCACTGCTTATGTACT	10724	0.3620234559061575	No Hit
CATAAGCAGTGGTATCAACGCAGAG	8961	0.3025076639663444	No Hit
CCCATGTACTCTGCGTTGATACCAC	8214	0.2772902524070475	No Hit
CTGCTTATGTACTCTGCGTTGATAC	8007	0.2703022949870014	No Hit
ACTCTGCGTTGATACCACTGCTTAT	7690	0.2596009302422931	No Hit
GGATACCACGTGTCCCGCCCTACTC	7684	0.25939838075185695	No Hit
ATACAGGGTGACAGCCCCGTACACA	7621	0.2572716111022777	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	7611	0.2569340286182175	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7198	0.24299187202653127	No Hit
GTATCAACGCAGAGTACATAAGCAG	6894	0.2327293645111012	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	6379	0.215343866582001	No Hit
CATGTACTCTGCGTTGATACCACTG	5852	0.19755326967202852	No Hit
TATCAACGCAGAGTACATAAGCAGT	5728	0.19336724686968204	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	5512	0.18607546521398172	No Hit
GGTATCAACGCAGAGTACATAAGCA	5484	0.18513023425861316	No Hit
GTACATGATAAGCAGTGGTATCAAC	5216	0.17608302368579984	No Hit
ATCATGTACTCTGCGTTGATACCAC	5095	0.17199827562867143	No Hit
ATCATTAACTGAATCCATAGGTTAA	5014	0.1692638575077838	No Hit
CCCATATTCAGACAGGATACCACGT	4444	0.15002165591635247	No Hit
GGTATCAACGCAGAGTACATGGGGG	4340	0.1465107980821264	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4257	0.14370886346442674	No Hit
GTGGTATCAACGCAGAGTACATAAG	4144	0.13989418139454649	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4133	0.13952284066208026	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	4006	0.13523554311451574	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	4003	0.13513426836929768	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	3989	0.13466165289161341	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	3721	0.12561444231880006	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	3631	0.12257619996225827	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	3597	0.1214284195164536	No Hit
TATTCAGACAGGATACCACGTGTCC	3553	0.11994305658658871	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	3464	0.11693857247845295	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	3428	0.11572327553583625	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	3300	0.1114022197398657	No Hit
ACATAAGCAGTGGTATCAACGCAGA	3267	0.11028819754246703	No Hit
GTATCAACGCAGAGTACATGGGGGT	3205	0.1081951861412938	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3109	0.10495439429431588	No Hit
GGTATCAACGCAGAGTACATGGGCA	3103	0.10475184480387975	No Hit
GTTAATGATAGTGTGTCGAAACACA	3064	0.103435273116045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.751649681203981E-5	0.0	0.0	0.0	0.0
7	6.751649681203981E-5	0.0	0.0	0.0	0.0
8	6.751649681203981E-5	0.0	0.0	0.0	0.0
9	6.751649681203981E-5	0.0	0.0	0.0	0.0
10	6.751649681203981E-5	0.0	0.0	0.0	0.0
11	6.751649681203981E-5	0.0	0.0	3.3758248406019906E-5	0.0
12	6.751649681203981E-5	0.0	0.0	3.3758248406019906E-5	0.0
13	6.751649681203981E-5	0.0	0.0	3.3758248406019906E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACCG	35	0.002173003	16.283268	12
ATCCGTT	45	6.769993E-4	14.776308	7
GTACAAA	500	0.0	14.25966	1
CCTAGTT	95	4.9021764E-9	14.001207	2
GATCGTA	165	0.0	13.816107	11
AATCGCC	220	0.0	12.952161	18
ATCGCCG	200	0.0	12.837381	19
CCCCTAG	75	1.4693804E-5	12.675253	1
TCGTTGA	150	1.8189894E-12	12.664979	14
CTAGTTC	115	5.324182E-9	12.392373	3
GACCGAA	55	0.0030540354	12.099104	1
CTAGTAG	55	0.0030675924	12.091951	3
TCTAAGG	110	3.814057E-8	12.091951	2
GACAGCG	95	1.0420699E-6	11.998806	7
ATCGTAA	175	0.0	11.941064	12
GACAACG	80	2.8402274E-5	11.886465	19
GGATCGT	200	0.0	11.873617	10
GATCGAA	80	2.8768025E-5	11.871612	15
CTAGGAC	105	2.7198257E-7	11.762918	3
GTACGAT	65	7.9816836E-4	11.700233	1
>>END_MODULE