FastQCFastQC Report
Wed 25 May 2016
SRR1295266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962239
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT638482.155396644227559No Hit
GTACATAAGCAGTGGTATCAACGCA563591.9025811219148758No Hit
GGTATCAACGCAGAGTACTTTTTTT431991.458322572891654No Hit
TATCAACGCAGAGTACTTTTTTTTT397301.3412152091711709No Hit
GCTTATGTACTCTGCGTTGATACCA351051.185083310293329No Hit
CTTATGTACTCTGCGTTGATACCAC236950.7999016959806416No Hit
ACGCAGAGTACTTTTTTTTTTTTTT221200.7467324547411603No Hit
GTACTTTTTTTTTTTTTTTTTTTTT183340.6189237262759689No Hit
GAGTACATAAGCAGTGGTATCAACG160270.541043447203281No Hit
GTGGTATCAACGCAGAGTACATGGG133130.44942356102934305No Hit
TATGTACTCTGCGTTGATACCACTG122850.41472008166795454No Hit
GCGTTGATACCACTGCTTATGTACT122580.41380860896099203No Hit
GTACTGGTTCACTATCGGTCAGTCA122560.41374109246418No Hit
CATAAGCAGTGGTATCAACGCAGAG116860.39449889087274864No Hit
GAGTACTTTTTTTTTTTTTTTTTTT114320.38592429577761955No Hit
CCCATGTACTCTGCGTTGATACCAC101880.3439290347605308No Hit
ACGCAGAGTACATAAGCAGTGGTAT92810.31331030345627076No Hit
CTGCTTATGTACTCTGCGTTGATAC92070.3108121930742253No Hit
ACTCTGCGTTGATACCACTGCTTAT90370.3050732908452019No Hit
ATACAGGGTGACAGCCCCGTACACA89550.30230511447590824No Hit
GGATACCACGTGTCCCGCCCTACTC86190.29096234301148555No Hit
GCAGAGTACTTTTTTTTTTTTTTTT83920.2832992206233191No Hit
GTATCAACGCAGAGTACATAAGCAG74290.2507900274083219No Hit
TATCAACGCAGAGTACATAAGCAGT67110.2265516050527996No Hit
CATGTACTCTGCGTTGATACCACTG66360.2240197364223481No Hit
ACCCTGTATCGCGCGCCTTTCCAGA64330.21716681199592608No Hit
GGTATCAACGCAGAGTACATAAGCA61390.2072418869645562No Hit
ATCATGTACTCTGCGTTGATACCAC58000.19579784075491546No Hit
ATCAACGCAGAGTACTTTTTTTTTT57590.19441375257026863No Hit
GTGGTATCAACGCAGAGTACTTTTT56660.19127423546850877No Hit
ATCATTAACTGAATCCATAGGTTAA55000.18567036623310948No Hit
GTACATGATAAGCAGTGGTATCAAC53200.1795938815200259No Hit
CCCATATTCAGACAGGATACCACGT52580.17750087011885268No Hit
GGTATCAACGCAGAGTACATGGGGG50540.17061418744402462No Hit
AAGCAGTGGTATCAACGCAGAGTAC48480.1636599882723845No Hit
GTGGTATCAACGCAGAGTACATAAG47330.1597777897056922No Hit
GGTTAATGAGGCGAACCGGGGGAAC42780.14441778668095315No Hit
ACATAAGCAGTGGTATCAACGCAGA42570.14370886346442674No Hit
CTATCGGTCAGTCAGGAGTATTTAG42410.14316873148993042No Hit
TCTAAGTACCCCGAGGAAAAGAAAT41420.13982666489773446No Hit
GTATTTAGCCTTGGAGGATGGTCCC41310.13945532416526824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40790.13769989524815518No Hit
GTCAGGAGTATTTAGCCTTGGAGGA40540.1368559390380047No Hit
TATTCAGACAGGATACCACGTGTCC39960.13489796063045556No Hit
GCCTTGGAGGATGGTCCCCCCATAT39860.13456037814639532No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG39830.13445910340117728No Hit
GCCCAGAGCCTGAATCAGTGTGTGT38310.12932784964346225No Hit
CACACACACTGATTCAGGCTCTGGG37970.1281800691976576No Hit
GTTCACTATCGGTCAGTCAGGAGTA37200.12558068407039405No Hit
CGGTACTGGTTCACTATCGGTCAGT35770.1207532545483332No Hit
GTATCAACGCAGAGTACATGGGGGT35110.11852521015353588No Hit
CCACTGCTTATGTACTCTGCGTTGA34990.11812011117266363No Hit
GATACCACTGCTTATGTACTCTGCG34740.11727615496251315No Hit
GGTATCAACGCAGAGTACATGGGCA34620.11687105598164091No Hit
TCACGGTACTGGTTCACTATCGGTC34560.11666850649120479No Hit
GCAGTGGTATCAACGCAGAGTACAT34450.11629716575873858No Hit
CATCTAAGTACCCCGAGGAAAAGAA33490.11305637391176068No Hit
GTTAATGATAGTGTGTCGAAACACA33290.11238120894364026No Hit
GTACATGGGAAGCAGTGGTATCAAC31620.10674358145983494No Hit
GGTATCAACGCAGAGTACATGGGGA31540.10647351547258678No Hit
GGTATCAACGCAGAGTACATGGGAT31110.10502191079112794No Hit
GCCTGAATCAGTGTGTGTGTTAGTG30410.10265883340270653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCG250.006001537719.0173932
CGGTAAA250.006049741518.99107214
TCTAGTA350.002157596216.3006232
CGTACTT508.75321E-515.19285813
GGTCAAT400.00520114614.2825991
TCCGCAA400.005296497614.24354417
AAGTGCG400.00529709114.2433027
CCGATGC759.732667E-713.9267859
CGATGCT759.732667E-713.92678510
TGTCGGA551.9689304E-413.81168712
CTTAGAC903.599598E-813.7347853
AGACCGC500.00150523813.2939756
GTATGCG500.001505429513.293759
CCGGTAA500.001505429513.293759
CGCACGT500.001505429513.2937514
GCGCTAA500.001505429513.293759
ACAGTCG1102.7703209E-912.9484588
AGTCGCC905.441416E-712.66071410
ACGGTAA604.1102376E-412.66071313
TCCGGGA604.1102376E-412.66071312
Produced by FastQC (version 0.11.5)