FastQCFastQC Report
Wed 25 May 2016
SRR1295265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884534
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG50670.17566095598110476No Hit
GTATCAACGCAGAGTACTTTTTTTT30610.10611766059959772No Hit
ATTCCATTCCATTCCATTCCATTCC29120.10095218153088159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG550.00306167912.095055
GTCTTAT3600.011.6247681
GGCTACG803.7320232E-410.700071
GTAGGAC1951.4551915E-1110.2339183
GGTATCA28500.09.7781541
GTCTTAG2850.09.6780371
TAGGACT2850.09.6699774
GATACCG900.00111063849.5032535
TAGGACG900.00111063849.5032534
GTGGTAT13850.09.4768381
GTCCTAC2002.6029738E-99.0356151
TGGTATC13750.08.9841152
CGTAGGA850.00742240068.9434622
AGTACTG3850.08.6393215
GTGCTAG3000.08.5600571
GACCATG2803.6379788E-128.4810747
TCTTATA4750.08.4021472
CTAGGAC2502.3464963E-108.3625733
GTTATGC1606.426364E-68.3150583
TAGTACT3700.08.2190294
Produced by FastQC (version 0.11.5)