##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295265_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2884534
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45471573571329	31.0	30.0	33.0	27.0	34.0
2	30.27421656322997	31.0	30.0	34.0	26.0	34.0
3	30.783484958055617	31.0	31.0	34.0	27.0	34.0
4	34.02207982294541	35.0	35.0	37.0	28.0	37.0
5	34.28521452685252	35.0	35.0	37.0	30.0	37.0
6	34.426730972836516	37.0	35.0	37.0	30.0	37.0
7	34.47894945942742	37.0	35.0	37.0	30.0	37.0
8	34.55350257615268	37.0	35.0	37.0	31.0	37.0
9	35.57045158767413	39.0	35.0	39.0	29.0	39.0
10	35.518518069123125	39.0	35.0	39.0	28.0	39.0
11	35.88909993780624	39.0	35.0	39.0	30.0	39.0
12	35.9120481852528	39.0	35.0	39.0	30.0	39.0
13	35.81066473822115	39.0	35.0	39.0	30.0	39.0
14	36.06490060439572	39.0	36.0	41.0	27.0	41.0
15	36.68542613815611	39.0	36.0	41.0	29.0	41.0
16	36.777065550276056	40.0	36.0	41.0	30.0	41.0
17	36.569196618933944	39.0	36.0	41.0	28.0	41.0
18	36.527777103684684	39.0	36.0	41.0	28.0	41.0
19	36.60051190244248	40.0	36.0	41.0	28.0	41.0
20	36.601097785638856	40.0	36.0	41.0	28.0	41.0
21	36.59371531068796	39.0	36.0	41.0	28.0	41.0
22	36.34214503971872	39.0	36.0	41.0	27.0	41.0
23	36.03044235221356	39.0	35.0	41.0	27.0	41.0
24	36.36381543777955	39.0	36.0	41.0	27.0	41.0
25	35.163421890676275	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4485912028164094
1101	2	0.6330405981958016
1101	3	0.48211060596888444
1101	4	0.44388714915668004
1101	5	0.45040173198866995
1101	6	0.35946792188899934
1101	7	0.41377386963953455
1101	8	0.4980348821681133
1101	9	0.519654398570836
1101	10	0.651092355472116
1101	11	0.6305836350685681
1101	12	0.5624239168304968
1101	13	0.6446023017041966
1101	14	1.2041876230435662
1101	15	0.8757526085083782
1101	16	0.6902724791410293
1101	17	0.915104768050341
1101	18	1.042455988172513
1101	19	0.9459731507833098
1101	20	1.0123066550576638
1101	21	1.0497155438680608
1101	22	0.7812390988067435
1101	23	-1.4076021533769563
1101	24	0.23580778005857894
1101	25	0.6716972103766778
1102	1	0.29001399632545244
1102	2	0.3965209190748631
1102	3	0.3918480834681297
1102	4	0.4505631815070643
1102	5	0.18300680138868586
1102	6	0.12983474201567446
1102	7	0.21225675564216573
1102	8	0.35731324728959635
1102	9	0.5496314839640988
1102	10	1.0560071769648758
1102	11	0.6681796594253129
1102	12	0.5520896006969167
1102	13	0.6245544442534836
1102	14	1.0830077184005447
1102	15	0.9370903801020205
1102	16	0.7815962842333946
1102	17	0.9860878516569613
1102	18	0.9197159440583533
1102	19	0.8775756968175799
1102	20	1.0024169479142415
1102	21	0.9575644118581579
1102	22	0.8624202263575143
1102	23	0.9672565411242857
1102	24	0.9916083894245631
1102	25	0.978558878631631
1103	1	-0.3321270328442125
1103	2	-1.47831005140673
1103	3	-0.32737871439906385
1103	4	-0.05736603380277927
1103	5	-0.2246434617930575
1103	6	-0.08893924479018978
1103	7	0.01957742194755241
1103	8	0.20476784651654611
1103	9	0.7624823858252583
1103	10	0.7581587881089362
1103	11	0.5328511224559307
1103	12	0.39849796917528124
1103	13	0.29104146808600007
1103	14	-0.9536887823678839
1103	15	-0.007055087000694016
1103	16	0.0850529913075917
1103	17	0.5591470679754309
1103	18	0.7410981140298176
1103	19	0.713606806796605
1103	20	0.678873721660004
1103	21	0.7817752085491918
1103	22	0.37251861820512033
1103	23	1.1503095262154943
1103	24	0.9370706199816752
1103	25	0.8475169815349233
1104	1	0.24992642988603464
1104	2	0.30951195742025917
1104	3	-0.05330778344970355
1104	4	0.04930606284789718
1104	5	0.06444195164301192
1104	6	0.17380133704063638
1104	7	0.22505458824255697
1104	8	0.26867176220579836
1104	9	-1.606201615511445
1104	10	-0.2021545999734471
1104	11	-0.21917055255907059
1104	12	0.3244567680713146
1104	13	0.3411251031947202
1104	14	-1.2227188427472484
1104	15	-0.1091773335753885
1104	16	0.18533180535537497
1104	17	0.6150277952958376
1104	18	0.7011670162473109
1104	19	0.7463613111185836
1104	20	0.8822586443942768
1104	21	1.060724474772904
1104	22	1.0895781316190636
1104	23	1.4617731360652257
1104	24	1.158491269521889
1104	25	1.169037519713413
1105	1	-0.1207332568978643
1105	2	0.029398011440537886
1105	3	-1.3231203081887344
1105	4	-0.43165908329653746
1105	5	-0.3968105133850912
1105	6	-0.140346193671256
1105	7	-0.052740545068651556
1105	8	-0.0241771269118658
1105	9	0.43375491479397965
1105	10	0.6409431438269309
1105	11	0.05317910957010952
1105	12	0.06311586378096479
1105	13	-0.18512400708766563
1105	14	0.677599059347429
1105	15	0.26976027702916383
1105	16	-0.10726214971233361
1105	17	0.45581582329109693
1105	18	0.3980533152172825
1105	19	0.24156859535430186
1105	20	-0.17575405038953562
1105	21	0.19056888734627364
1105	22	0.4030278663456528
1105	23	0.42664681835127993
1105	24	0.41215264610363533
1105	25	0.5427757181972126
1106	1	-1.3829300488908274
1106	2	-0.9097139367060976
1106	3	-1.0271812771183235
1106	4	-1.3849988327711884
1106	5	-0.94240336866023
1106	6	-0.680221064600893
1106	7	-0.5267718731430975
1106	8	-0.6124922742858914
1106	9	-0.2321121995074975
1106	10	-1.7782168156339964
1106	11	-0.9002241014907213
1106	12	-0.7744902315850979
1106	13	-0.8768996718119979
1106	14	0.025565616522975176
1106	15	-0.7352216960631281
1106	16	-1.2270134470961906
1106	17	-0.5130068831690053
1106	18	-0.45602511757737574
1106	19	-0.33846195209492436
1106	20	-0.3267926254908389
1106	21	-0.29732307145045667
1106	22	-0.13594112622791243
1106	23	-0.45196548193558783
1106	24	-0.7991863647354123
1106	25	-1.3327951975009853
1107	1	0.016446552648048396
1107	2	0.17645956086253634
1107	3	0.05755454980885233
1107	4	0.1402189590366234
1107	5	0.0045308533196148915
1107	6	0.1650150017923977
1107	7	0.0449888669994607
1107	8	-0.09047581230157675
1107	9	0.5566966051545776
1107	10	0.1840056571245441
1107	11	0.12454338040948443
1107	12	-0.06176566655693705
1107	13	0.18316633868256105
1107	14	-1.188372665893219
1107	15	-0.44404925599083356
1107	16	-0.19495186235489825
1107	17	0.10175594050352998
1107	18	0.3170027391484709
1107	19	0.2943121754268958
1107	20	0.2881106878811579
1107	21	0.5133842100673078
1107	22	-0.22841502966538485
1107	23	0.5564105882332271
1107	24	0.3609181438696325
1107	25	0.1883269809065098
1108	1	-0.30390652605174395
1108	2	-0.036529848184841285
1108	3	-0.3163391744883697
1108	4	-0.5329381870396546
1108	5	-0.5592197804474992
1108	6	-0.5364123825803091
1108	7	-0.4768239886143064
1108	8	-0.3710902847256605
1108	9	0.06527076999036296
1108	10	0.08290712746424589
1108	11	-0.13994171902152885
1108	12	-0.2295343838123003
1108	13	-0.36017206478766184
1108	14	-0.11129461395248086
1108	15	-0.39793495257130473
1108	16	-0.2429505666532492
1108	17	-0.06378402907564862
1108	18	-0.05203703325871345
1108	19	-0.13854512838697275
1108	20	-0.04246082921646632
1108	21	-0.032285048002663075
1108	22	-0.1446296091257011
1108	23	-0.22920853181634726
1108	24	-0.21018418839333464
1108	25	-1.0836717797266928
1109	1	-0.7835612985891238
1109	2	-0.6447175660678113
1109	3	-0.7572457116977382
1109	4	-0.6087603801307893
1109	5	-0.858234805368987
1109	6	-1.1635724851473697
1109	7	-0.9077806569135944
1109	8	-1.2810578003556188
1109	9	-0.8493097422687086
1109	10	-1.1106082431888993
1109	11	-1.1293879001272984
1109	12	-1.040925161420418
1109	13	-1.0247219653898156
1109	14	-0.44864155566788355
1109	15	-1.2077911858249308
1109	16	-1.3301747016038377
1109	17	-1.330912151464652
1109	18	-1.9520165054417191
1109	19	-2.266144238127964
1109	20	-1.879229750821814
1109	21	-1.67897034576405
1109	22	-1.8374985336121412
1109	23	-1.2583515494774673
1109	24	-2.152029603127268
1109	25	-1.1575052955295106
1110	1	-0.08182526973504167
1110	2	-0.010278285069230009
1110	3	-0.2089428540196927
1110	4	0.04954228472148259
1110	5	-0.008231591606005395
1110	6	0.03210004525941912
1110	7	-0.025901964529481347
1110	8	-0.057690529685153535
1110	9	0.17243926100565687
1110	10	0.10251630573650772
1110	11	0.004198310442127706
1110	12	0.04926698487741987
1110	13	-0.025790587493524697
1110	14	-2.6746157849616097
1110	15	-0.8822528346138938
1110	16	-0.461827650093646
1110	17	-0.4952013680961471
1110	18	-2.969392541421172
1110	19	-0.6828731599747542
1110	20	-2.27305271471932
1110	21	-0.5266625425638765
1110	22	-1.687124727065985
1110	23	-0.5649927447769301
1110	24	-0.08497241834691494
1110	25	-0.13348099397202873
1111	1	-0.36710900339062746
1111	2	-1.3639650971978483
1111	3	-0.4556874124179977
1111	4	-0.00583585420644539
1111	5	-0.15371015595482618
1111	6	-0.009313420393780802
1111	7	-0.11770094445365231
1111	8	-0.09070390490391134
1111	9	-1.7718808434258477
1111	10	-0.381357276432567
1111	11	-0.18409589412922145
1111	12	-0.260234493055826
1111	13	-0.10269882253669493
1111	14	0.739692380536944
1111	15	0.10764424552591834
1111	16	0.16670979315896517
1111	17	-0.27599475844901633
1111	18	-0.036350475743383015
1111	19	-0.19118365332284526
1111	20	0.14665604406796007
1111	21	-0.5996656579687141
1111	22	-0.2703700278464467
1111	23	-0.05506346609737989
1111	24	-0.6137179225167273
1111	25	-2.1611530078174894
1112	1	0.1285409839032674
1112	2	0.3308877536492929
1112	3	-0.04103578375212891
1112	4	0.3507767549541043
1112	5	0.19628370165072795
1112	6	0.15253885080645802
1112	7	0.18077241551594625
1112	8	-0.002177276847845633
1112	9	-0.34216704352776617
1112	10	0.458807818712458
1112	11	8.934243133538189E-4
1112	12	0.06305085864171645
1112	13	0.10163970367004538
1112	14	0.41966675544297516
1112	15	0.13530400210969873
1112	16	0.08715988653666074
1112	17	-3.8986868923257205
1112	18	-1.1046647676539152
1112	19	-1.0556765775948804
1112	20	-0.11245423563939028
1112	21	-0.6047562172636631
1112	22	-1.6737898115011092
1112	23	-1.741435954201755
1112	24	-0.5504082407282382
1112	25	-0.08022308034209402
1113	1	-0.4445972392952413
1113	2	-0.2430011811176378
1113	3	-0.18092204291167846
1113	4	-0.531444109278091
1113	5	-0.1774832342282906
1113	6	-0.18621154145079544
1113	7	-0.41363413366623547
1113	8	-0.41832120910983406
1113	9	0.062006243340292144
1113	10	0.19474430882206661
1113	11	-0.2389231369255711
1113	12	-0.32165501723432754
1113	13	-0.6082037788036274
1113	14	-1.5742797654416236
1113	15	-1.835517563505988
1113	16	-0.6211689599242121
1113	17	-0.37733565554547965
1113	18	-0.7053240901636997
1113	19	-0.2886449052301643
1113	20	0.11931348200145209
1113	21	-1.6115027117225154
1113	22	-2.0586844112886027
1113	23	-0.665166389510965
1113	24	-2.534616211395651
1113	25	-0.8123472153116253
1114	1	0.0486772476013293
1114	2	0.16521184653375443
1114	3	-0.14594733334391918
1114	4	0.3106598505412421
1114	5	-0.07061038313840129
1114	6	0.1257873402006311
1114	7	0.055306064640930686
1114	8	-0.1045385614861587
1114	9	0.23277057023978642
1114	10	0.18498409917609138
1114	11	0.07010794939251497
1114	12	0.07978046303980335
1114	13	0.07712280005936378
1114	14	0.7165877868062225
1114	15	0.2632996275529891
1114	16	0.18041829622753625
1114	17	-0.6006605901697952
1114	18	-0.26710026734206593
1114	19	-0.7122642495539324
1114	20	-1.909115293728604
1114	21	-0.9265646258515403
1114	22	-1.5894564601187184
1114	23	-3.1405749473183775
1114	24	-2.1704829370862697
1114	25	-1.8184288569763183
1115	1	0.14686110200661773
1115	2	0.24734978646752737
1115	3	0.10368537692870916
1115	4	0.29005551835742693
1115	5	0.2674423716233463
1115	6	0.24283585796017348
1115	7	0.18015379182487123
1115	8	0.2412672325166909
1115	9	0.4574926908481487
1115	10	0.7133225289873479
1115	11	0.36220124631059747
1115	12	0.20225397733657502
1115	13	0.11913825878381346
1115	14	0.720048774777112
1115	15	0.30595258996217467
1115	16	0.2571673470751108
1115	17	0.2206548812218614
1115	18	0.4497397742821292
1115	19	-0.19497825131313817
1115	20	0.3587664774543313
1115	21	-0.6300928565509238
1115	22	0.43494507367844903
1115	23	0.5667623235028927
1115	24	0.5243220885973727
1115	25	0.4798453252491086
1116	1	0.32829218026193985
1116	2	0.49059932881661084
1116	3	0.19590149166246107
1116	4	0.5825877662314412
1116	5	0.4802702836944661
1116	6	0.4111275163255854
1116	7	0.23058903647120133
1116	8	0.0877023892060862
1116	9	0.35965292449512276
1116	10	0.3039624327351689
1116	11	0.21599511885910516
1116	12	0.25199542376179807
1116	13	-0.2540166597242788
1116	14	0.6544584937149054
1116	15	0.34819248529342417
1116	16	0.31936901147050634
1116	17	0.5097170953973702
1116	18	0.5168809597558166
1116	19	0.6539027336680263
1116	20	0.47994013761338294
1116	21	-0.023730556257234525
1116	22	-0.027499022013387275
1116	23	-0.8633521968029285
1116	24	-0.3655045547329081
1116	25	-1.012751790862957
1201	1	0.3772544585169122
1201	2	0.648042285024335
1201	3	0.5883992382580381
1201	4	0.3218344574552603
1201	5	0.3843386806786384
1201	6	0.2939711504453797
1201	7	0.24875962618961012
1201	8	0.3533310398067755
1201	9	0.6236560450985138
1201	10	1.0056695651427034
1201	11	0.5572758883811133
1201	12	0.5679813877034476
1201	13	0.5793588185202978
1201	14	1.1354337414460858
1201	15	0.7977776175672702
1201	16	0.6598246961194434
1201	17	0.6825668751235625
1201	18	0.5689217735350667
1201	19	0.9069855453213265
1201	20	0.975106586282223
1201	21	0.8206951328028183
1201	22	0.916212835493603
1201	23	0.4975809682712722
1201	24	0.8048056666392895
1201	25	0.9175731477896178
1202	1	0.47221161755999574
1202	2	0.7669238932334288
1202	3	0.5592322211851446
1202	4	0.476691161396559
1202	5	0.3801915884553324
1202	6	0.3302725150146486
1202	7	0.3766126613086911
1202	8	0.4798943653124752
1202	9	0.9015713068432234
1202	10	0.18072121517420214
1202	11	0.37695457338336524
1202	12	0.45411913019912475
1202	13	0.42870317089506216
1202	14	1.1263045167200971
1202	15	0.8110933149627684
1202	16	0.8017865121763279
1202	17	-1.2255523331675278
1202	18	0.22962523159829118
1202	19	0.7236619773572102
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2210	22	1.1170744996711903
2210	23	1.4628088607674243
2210	24	1.0173505762439135
2210	25	1.0180013112122595
2211	1	0.009610410495014321
2211	2	-0.653527580638599
2211	3	-0.088943462589544
2211	4	0.2933983420752213
2211	5	0.2462498749062263
2211	6	0.13760458164054512
2211	7	0.20580575965519898
2211	8	0.31051945690205685
2211	9	0.6599553544811059
2211	10	0.7485150581683087
2211	11	0.37723848950277983
2211	12	0.23523411372643466
2211	13	0.10476316917952033
2211	14	-1.0029802777329735
2211	15	0.009610476186921346
2211	16	0.10252519429791107
2211	17	0.22831000058406659
2211	18	0.10768353620499482
2211	19	0.4398159338140175
2211	20	0.5206428984842617
2211	21	0.6033407670859461
2211	22	-0.07575280555297326
2211	23	0.5110751795566699
2211	24	-0.1418253228237134
2211	25	0.2942260008108022
2212	1	-0.07744536768168686
2212	2	-1.1876712424788067
2212	3	-0.377579784185496
2212	4	0.18242743031954944
2212	5	0.15936544229763427
2212	6	0.07692133669475254
2212	7	0.060454747585438895
2212	8	0.04524535177171174
2212	9	-2.308923179351382
2212	10	-0.17823302387031958
2212	11	-0.10369029728030199
2212	12	-0.07257713139959776
2212	13	-0.005460993254502
2212	14	0.6872655963630621
2212	15	0.21814877181181203
2212	16	0.2128459660008275
2212	17	0.33875262971326947
2212	18	0.05591118130672612
2212	19	-0.5288174386568798
2212	20	0.18908045746785973
2212	21	0.22934581689276
2212	22	-1.67335806165827
2212	23	-0.3492772180933912
2212	24	0.29899983430004085
2212	25	-0.4077227887372956
2213	1	0.08896000658048564
2213	2	0.3253867583381691
2213	3	0.051790701443856335
2213	4	0.3122644970858559
2213	5	0.3148784942887275
2213	6	0.17797913544560373
2213	7	0.054573578145152624
2213	8	0.0939830046168808
2213	9	0.540263896429515
2213	10	0.5064121279797646
2213	11	-0.13382210187887011
2213	12	-0.11325478454089932
2213	13	-0.0582925754932333
2213	14	-1.7903061959025095
2213	15	-0.43000097312325636
2213	16	-0.28620545547340015
2213	17	-2.26958015926089
2213	18	-0.9826230902405371
2213	19	-0.6553763309164466
2213	20	0.16642797375290996
2213	21	-0.25258868755312847
2213	22	0.25966851439218885
2213	23	0.7163502886806157
2213	24	0.5893307959693104
2213	25	-1.078004958404911
2214	1	0.10035829523855
2214	2	0.08961332619124462
2214	3	-0.023788016599478823
2214	4	0.4189673640210003
2214	5	0.3425563349906753
2214	6	0.25116336026383834
2214	7	0.19551403021332447
2214	8	0.12700051813980195
2214	9	0.46139646925524147
2214	10	0.45644296837041765
2214	11	-0.30204855072223324
2214	12	-0.341111406254754
2214	13	-0.2693718470649671
2214	14	0.6370276711982754
2214	15	0.2671286280121308
2214	16	0.19547579654432212
2214	17	0.12652626917319765
2214	18	0.13629097275557456
2214	19	-0.9816625128671959
2214	20	-0.0587525724217457
2214	21	0.24048095044862805
2214	22	0.5567930975806448
2214	23	0.9022585614988401
2214	24	0.6409088958726414
2214	25	0.6444576367973127
2215	1	-0.23632333808799189
2215	2	0.17702415499264745
2215	3	0.08549929366419207
2215	4	0.34524863557643215
2215	5	0.2865915852717009
2215	6	0.30737417359367925
2215	7	0.20043068473759007
2215	8	0.1004845809033057
2215	9	0.3277968215166567
2215	10	0.604806067003473
2215	11	0.24426853492998646
2215	12	0.15604036858762527
2215	13	-0.01019581634898259
2215	14	0.8255759475574251
2215	15	0.2735956387319973
2215	16	0.30847404049504235
2215	17	0.501624320858717
2215	18	0.2699962330807679
2215	19	-0.44102409255351915
2215	20	-1.8860597170949447
2215	21	-2.0769538176629396
2215	22	-0.25664812030530015
2215	23	0.3350713314595808
2215	24	0.48417359946497385
2215	25	-0.34408111156918153
2216	1	-0.08883055490477787
2216	2	-0.30856548117884586
2216	3	-0.1696654180203474
2216	4	0.22235502688775455
2216	5	0.2733672209440954
2216	6	0.09024988517325738
2216	7	0.09659923477016719
2216	8	-0.040419147828373525
2216	9	0.3063767250639131
2216	10	-1.67410467408844
2216	11	-0.589890523224355
2216	12	-0.20395693657228975
2216	13	-0.056218212022997704
2216	14	-0.4943653909060117
2216	15	-0.5566470479042636
2216	16	-0.07246977563998058
2216	17	0.2519533522166313
2216	18	0.12272128122641845
2216	19	-1.3384243530515718
2216	20	-0.961976136714874
2216	21	-0.8425052393733594
2216	22	-0.7937848110501946
2216	23	-4.469892694650444
2216	24	-2.1436058745554547
2216	25	-1.1974529781488386
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	11.0
4	24.0
5	40.0
6	60.0
7	211.0
8	122.0
9	296.0
10	819.0
11	1893.0
12	3673.0
13	5959.0
14	8606.0
15	11145.0
16	13252.0
17	14451.0
18	15284.0
19	15498.0
20	16002.0
21	16263.0
22	16780.0
23	17756.0
24	19540.0
25	21828.0
26	24808.0
27	29101.0
28	34264.0
29	43162.0
30	53741.0
31	67646.0
32	90939.0
33	123571.0
34	172398.0
35	242953.0
36	352921.0
37	539199.0
38	910315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.191911909105606	20.965860476025583	13.819231300358	23.022996314510806
2	15.794543504560949	21.342303009876424	40.45016208733958	22.412991398223056
3	18.061379623169145	26.33429138571272	30.508442836593346	25.095886154524788
4	13.134357759967594	17.630001571078832	38.09959807433596	31.136042594617614
5	13.64943201505886	37.38104330531822	35.15717701200137	13.812347667621546
6	30.577840991440496	38.25438290512703	17.73602085403631	13.43175524939616
7	25.782946118133086	32.79862601915543	24.097926302835447	17.320501559876043
8	23.335907635829713	37.20280937665698	21.412116468519354	18.049166518993946
9	24.44750389704518	17.641485714880208	22.33153407465334	35.57947631342127
10	15.379149852954793	29.729277999533256	33.60599374368673	21.28557840382522
11	31.42339089710577	26.062685124246066	23.86189549196671	18.652028486681456
12	20.913551223639224	27.65400378638151	31.352729870111855	20.07971511986741
13	25.87239835730804	24.587393799090993	27.094083749826186	22.446124093774785
14	21.058069017800136	23.593965286141888	27.981489271608247	27.366476424449726
15	22.671603561389016	30.890028207854503	24.331962127816883	22.106406102939598
16	22.14548854034321	31.130229803429856	25.650587269267263	21.073694386959684
17	20.35971726939716	30.291142892412847	28.341371126913916	21.00776871127607
18	20.38652750455904	30.11188935828764	29.055343395642808	20.44623974151051
19	21.154978394605827	28.817351721670097	29.26644988457012	20.761219999153955
20	22.349501252892125	28.444829232554596	28.56216109762157	20.64350841693171
21	22.857536134049376	28.868649822428544	27.484310158377166	20.789503885144917
22	21.891012505226982	28.358530328972144	29.03379039511469	20.71666677068619
23	22.09732165550213	28.764836685930533	28.303668918991548	20.834172739575788
24	21.708611990269315	27.975064422802205	28.631705449794598	21.684618137133885
25	22.39803206501293	28.180090483918214	28.03165548921426	21.3902219618546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2854.0
1	2854.0
2	2977.5
3	3101.0
4	3101.0
5	3101.0
6	4149.5
7	5198.0
8	5198.0
9	5198.0
10	8347.5
11	11497.0
12	11497.0
13	11497.0
14	19221.0
15	26945.0
16	26945.0
17	26945.0
18	42101.0
19	57257.0
20	57257.0
21	57257.0
22	80877.0
23	104497.0
24	104497.0
25	104497.0
26	135597.5
27	166698.0
28	166698.0
29	166698.0
30	200711.5
31	234725.0
32	234725.0
33	234725.0
34	266543.5
35	298362.0
36	298362.0
37	298362.0
38	323412.5
39	348463.0
40	348463.0
41	348463.0
42	350960.5
43	353458.0
44	353458.0
45	353458.0
46	342709.0
47	331960.0
48	331960.0
49	331960.0
50	316563.0
51	301166.0
52	301166.0
53	301166.0
54	272466.5
55	243767.0
56	243767.0
57	243767.0
58	209378.5
59	174990.0
60	174990.0
61	174990.0
62	144013.5
63	113037.0
64	113037.0
65	113037.0
66	86939.5
67	60842.0
68	60842.0
69	60842.0
70	44445.5
71	28049.0
72	28049.0
73	28049.0
74	19733.5
75	11418.0
76	11418.0
77	11418.0
78	7861.5
79	4305.0
80	4305.0
81	4305.0
82	2864.0
83	1423.0
84	1423.0
85	1423.0
86	912.5
87	402.0
88	402.0
89	402.0
90	249.5
91	97.0
92	97.0
93	97.0
94	54.0
95	11.0
96	11.0
97	11.0
98	11.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08389570031069143
2	0.02062724863010802
3	0.03140888614937456
4	0.04038780614130393
5	0.05016408196263244
6	0.01691781064116422
7	0.022568636736471123
8	0.03137421850461808
9	0.032552918426338534
10	0.025203377737963915
11	0.02288064553927948
12	0.01781916940483281
13	0.02534204831698985
14	0.035256994717344295
15	0.03258758607109502
16	0.023504663144896194
17	0.022811310249766514
18	0.024371354263808296
19	0.018443187010449523
20	0.014109731415889015
21	0.011891002151474032
22	0.01594711658798267
23	0.019101872260822716
24	0.016016451877495636
25	0.10878706924584698
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2884534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.80075729650699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.91925797614579	66.75464836218453
2	9.204506287432132	14.138261068304958
3	1.874565870777232	4.319042354336325
4	0.6906192888578527	2.121603375514328
5	0.3698439344465544	1.4202147123507534
6	0.21183793213485716	0.9761588167249851
7	0.14863326462767457	0.7990603098000272
8	0.10029992732001652	0.6162488299969504
9	0.07535688758288689	0.5208719430486112
>10	0.37510235243872675	5.002492965031992
>50	0.020949714120595105	1.1081709978930412
>100	0.007804373428664309	0.9928277066415877
>500	8.147937916968403E-4	0.4302501289384441
>1k	3.6213057372332265E-4	0.6239947930270558
>5k	4.526632171541533E-5	0.1761536362063676
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5067	0.17566095598110476	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3061	0.10611766059959772	No Hit
ATTCCATTCCATTCCATTCCATTCC	2912	0.10095218153088159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.466764475648406E-5	0.0	0.0	3.466764475648406E-5	0.0
9	3.466764475648406E-5	0.0	0.0	3.466764475648406E-5	0.0
10	3.466764475648406E-5	0.0	0.0	3.466764475648406E-5	0.0
11	3.466764475648406E-5	0.0	0.0	1.0400293426945219E-4	0.0
12	3.466764475648406E-5	0.0	0.0	1.0400293426945219E-4	0.0
13	3.466764475648406E-5	0.0	0.0	1.0400293426945219E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACCG	55	0.003061679	12.09505	5
GTCTTAT	360	0.0	11.624768	1
GGCTACG	80	3.7320232E-4	10.70007	1
GTAGGAC	195	1.4551915E-11	10.233918	3
GGTATCA	2850	0.0	9.778154	1
GTCTTAG	285	0.0	9.678037	1
TAGGACT	285	0.0	9.669977	4
GATACCG	90	0.0011106384	9.503253	5
TAGGACG	90	0.0011106384	9.503253	4
GTGGTAT	1385	0.0	9.476838	1
GTCCTAC	200	2.6029738E-9	9.035615	1
TGGTATC	1375	0.0	8.984115	2
CGTAGGA	85	0.0074224006	8.943462	2
AGTACTG	385	0.0	8.639321	5
GTGCTAG	300	0.0	8.560057	1
GACCATG	280	3.6379788E-12	8.481074	7
TCTTATA	475	0.0	8.402147	2
CTAGGAC	250	2.3464963E-10	8.362573	3
GTTATGC	160	6.426364E-6	8.315058	3
TAGTACT	370	0.0	8.219029	4
>>END_MODULE