FastQCFastQC Report
Wed 25 May 2016
SRR1295265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884534
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT124640.4320975242448174No Hit
GGTATCAACGCAGAGTACTTTTTTT106380.36879440491947746No Hit
TATCAACGCAGAGTACTTTTTTTTT75790.2627460796093927No Hit
GTGGTATCAACGCAGAGTACATGGG58460.2026670512464058No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51350.17801835582454567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC707.297009E-613.5661416
GTGGTAT15900.012.0370981
TGGTATC15950.011.5633852
GGTATCA37600.011.4971671
AGCGTCA1850.011.3056353
TAAGGCG600.00584629211.091325
GCCTAAC1106.670107E-59.5218831
GTCCTAG2002.5465852E-109.5218821
CTAGGAC2600.09.507013
GCAGCGT2106.4028427E-109.06845951
CCATCCG950.00183092338.9964949
GTATTAG3950.08.6781711
CTGCTCG1651.0565236E-68.6329999
CAGAACG1000.00288662438.556314
CCGTGCA1000.0029162338.5466699
ATTAGAC1803.9913357E-78.4506763
AGACTGT4850.08.4193996
CAGCGTC2502.3283064E-108.3661692
AGGACTC3300.08.3545015
TAGGACT3550.08.3018964
Produced by FastQC (version 0.11.5)