FastQCFastQC Report
Wed 25 May 2016
SRR1295264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652881
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG67160.18385488057234825No Hit
GTATCAACGCAGAGTACTTTTTTTT56770.15541157787510734No Hit
GGTATCAACGCAGAGTACTTTTTTT46780.12806330126823184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34200.010.6204741
GTGGTAT16500.010.1422751
TGGTATC15650.010.0787982
CGAGACT1252.5660847E-69.8819454
TTACCCG800.00451058489.5018695
GTCCCGA850.00738514968.9490651
GTCCTAG3200.08.9141081
GTATTAA6300.08.90467551
CCAGGAC5600.08.8231643
ATACCGT1107.1604614E-48.634876
ACACCGT1000.00291090168.548526
GTTCTAG4450.08.546861
CTATACT4800.08.5120914
CTTATAC3850.08.3912623
CAGGACT4350.08.3004844
TTACACT5850.08.2836814
GTTTAGG3450.08.2681581
AAGGCCG1050.00450687588.144465
GTATCAA59850.08.1341551
GTCCTGG7050.08.092241
Produced by FastQC (version 0.11.5)