##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295264_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3652881
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.098450236949958	31.0	30.0	33.0	26.0	34.0
2	29.92785037344496	31.0	30.0	33.0	25.0	34.0
3	30.303765165084766	31.0	30.0	34.0	26.0	34.0
4	33.6609027230835	35.0	35.0	37.0	28.0	37.0
5	33.990716368805884	35.0	35.0	37.0	29.0	37.0
6	34.3808309112725	36.0	35.0	37.0	30.0	37.0
7	34.345552181962674	37.0	35.0	37.0	30.0	37.0
8	34.315881081261615	37.0	35.0	37.0	30.0	37.0
9	35.40250969029651	39.0	35.0	39.0	28.0	39.0
10	35.32330782196299	39.0	35.0	39.0	28.0	39.0
11	35.71812769153991	39.0	35.0	39.0	30.0	39.0
12	35.73020391302098	39.0	35.0	39.0	30.0	39.0
13	35.64462543400675	39.0	35.0	39.0	30.0	39.0
14	35.87154796446969	39.0	35.0	41.0	27.0	41.0
15	36.49991198727799	39.0	36.0	41.0	28.0	41.0
16	36.59292760974146	39.0	36.0	41.0	29.0	41.0
17	36.3960520476851	39.0	36.0	41.0	27.0	41.0
18	36.37238032117663	39.0	36.0	41.0	27.0	41.0
19	36.44095496130315	39.0	36.0	41.0	27.0	41.0
20	36.43626414328855	39.0	36.0	41.0	27.0	41.0
21	36.43797183647647	39.0	36.0	41.0	27.0	41.0
22	36.18535835139442	39.0	35.0	41.0	27.0	41.0
23	35.87662313664201	39.0	34.0	41.0	27.0	41.0
24	36.207446943932744	39.0	35.0	41.0	27.0	41.0
25	35.00054094288864	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2643379158091257
1101	2	0.5076091139970842
1101	3	0.3002947625267218
1101	4	0.3641820155985016
1101	5	0.2678322832539237
1101	6	0.2564851853483816
1101	7	0.2547266174644278
1101	8	0.32838454204954814
1101	9	0.2739885520755294
1101	10	0.49136140508286985
1101	11	0.4538221500848607
1101	12	0.6153532819507035
1101	13	0.47294851134048344
1101	14	1.0706605503757984
1101	15	0.7863844778208957
1101	16	0.6716913171898184
1101	17	0.8829014529981976
1101	18	0.8372916981924519
1101	19	0.8365074081216761
1101	20	0.8461737109190324
1101	21	0.9558910482838883
1101	22	0.7299757475444295
1101	23	-1.5368865253963833
1101	24	0.1948764146324251
1101	25	0.6008702016001308
1102	1	0.09088806029289032
1102	2	0.07284388123378349
1102	3	0.13791094840809848
1102	4	0.28059656145887857
1102	5	0.101085407775102
1102	6	0.11772533793113382
1102	7	0.12210365365582021
1102	8	0.2171666948129598
1102	9	0.33495983814290753
1102	10	0.8564070007866746
1102	11	0.49425812017626924
1102	12	0.48030404524982373
1102	13	0.48329221098196484
1102	14	1.0302812454208734
1102	15	0.7183536036455536
1102	16	0.4912642507390004
1102	17	0.7482165934725558
1102	18	0.7350322144124632
1102	19	0.7797959961393133
1102	20	0.8472903655672184
1102	21	0.8285667371830385
1102	22	0.6479289769176901
1102	23	0.7933380664712786
1102	24	0.8522865835888709
1102	25	0.7244647258611607
1103	1	-0.3952619200157912
1103	2	-1.5799858275663894
1103	3	-0.48265868187982264
1103	4	-0.11604843900855144
1103	5	-0.20520321189232504
1103	6	-0.11507883541226249
1103	7	0.06906495565851856
1103	8	0.20012753611934642
1103	9	0.8441337574083434
1103	10	0.7553386749560858
1103	11	0.5568957971496218
1103	12	0.38465287713658114
1103	13	0.19731037496914894
1103	14	-1.0061122845233186
1103	15	-0.12211202822884815
1103	16	-0.01925551295204997
1103	17	0.39818436608968
1103	18	0.583896418916936
1103	19	0.5528805863241502
1103	20	0.6053115993296885
1103	21	0.6451885089612475
1103	22	0.2558252525560434
1103	23	1.0576078307784513
1103	24	0.9054878725527473
1103	25	0.7140756297082262
1104	1	0.06885651812184435
1104	2	0.09764727682787466
1104	3	-0.2227856844865066
1104	4	-0.22022090237341985
1104	5	-0.09235675239355601
1104	6	0.0609098372292749
1104	7	0.09421926986640727
1104	8	0.13176524370216214
1104	9	-1.9085824533327553
1104	10	-0.3354672125367131
1104	11	-0.2669696974856848
1104	12	0.21672497097718946
1104	13	0.27757206843487126
1104	14	-1.515983756469744
1104	15	-0.3916368332157205
1104	16	0.09984588899466473
1104	17	0.5020297736402739
1104	18	0.5717041320645748
1104	19	0.5788351499731164
1104	20	0.6177763309974793
1104	21	0.7899403004460837
1104	22	0.8494187417046248
1104	23	1.2537854879849064
1104	24	0.9754244261654961
1104	25	0.775252762160946
1105	1	-0.2501283769114373
1105	2	-0.1513682391125144
1105	3	-1.484629777245285
1105	4	-0.5685945582874012
1105	5	-0.6341868653102551
1105	6	-0.19393793408072213
1105	7	-0.11600437392048235
1105	8	-0.18590800528050977
1105	9	0.3262835086151199
1105	10	0.37608111392459875
1105	11	-0.08353095951171952
1105	12	-0.11749572862126456
1105	13	-0.24461821911195614
1105	14	0.5514195636585342
1105	15	0.2060860694711053
1105	16	-0.24661495397297273
1105	17	0.19667610047887507
1105	18	0.09006390919557816
1105	19	0.24847322365193492
1105	20	-0.37128045074181415
1105	21	0.12807815179488102
1105	22	0.1938030222985816
1105	23	0.3865114224447126
1105	24	0.27501294719926506
1105	25	0.6020744534860327
1106	1	-1.6205856315636034
1106	2	-1.0972321481685618
1106	3	-1.3231566827979897
1106	4	-1.6329983723176795
1106	5	-0.9996281631724955
1106	6	-0.8031888424152953
1106	7	-0.6953417934053121
1106	8	-0.8204131498015812
1106	9	-0.4663728581765554
1106	10	-2.052414398025114
1106	11	-1.379380374212765
1106	12	-1.0488915415075937
1106	13	-1.187478221841836
1106	14	-0.299288839817514
1106	15	-1.0744248077948555
1106	16	-1.6298688391750815
1106	17	-0.8441735417120455
1106	18	-0.7022344535272822
1106	19	-0.6969359656391418
1106	20	-0.4284732256633319
1106	21	-0.40219425108318774
1106	22	-0.4226459257232307
1106	23	-0.7286057348417856
1106	24	-0.8346969707093876
1106	25	-1.704589959947569
1107	1	-0.01246819071754146
1107	2	0.06684021560866071
1107	3	-0.054028438921953637
1107	4	0.09678141444002364
1107	5	-0.09341985595917635
1107	6	-0.021598303301352928
1107	7	0.0034944192967074628
1107	8	-0.11775480076086353
1107	9	0.518613224119953
1107	10	0.04801947051957001
1107	11	-0.06037066071659325
1107	12	-0.06827344105761313
1107	13	0.07438611543864226
1107	14	-1.1086440500691097
1107	15	-0.5839246176987984
1107	16	-0.3306920575668997
1107	17	-0.09979391854794528
1107	18	0.2363360763196738
1107	19	0.13864766404768858
1107	20	0.3183716790925786
1107	21	0.38407605733240047
1107	22	-0.29952046472681104
1107	23	0.49157125229351095
1107	24	0.24102583705615643
1107	25	-0.1142307300698917
1108	1	-0.19422230097641702
1108	2	0.10401614536845116
1108	3	-0.09610086321931632
1108	4	-0.3751207860557102
1108	5	-0.41803614695839286
1108	6	-0.2894686690647106
1108	7	-0.23256299299413286
1108	8	-0.2900648435215487
1108	9	0.21954832246849065
1108	10	0.2583975746805791
1108	11	-0.05456688399632981
1108	12	-0.19870454456973619
1108	13	-0.2521552700176102
1108	14	-0.03286221466241557
1108	15	-0.19040409138273873
1108	16	-0.1869556216779955
1108	17	0.12030561133187945
1108	18	0.079024094691313
1108	19	0.026200655794831107
1108	20	0.10463009718429817
1108	21	0.08282680904892459
1108	22	0.13331012311594037
1108	23	-0.11214103799271413
1108	24	0.009212499081250769
1108	25	-0.799438733527424
1109	1	-0.7965784451347986
1109	2	-0.6370413333637295
1109	3	-0.7889646638400407
1109	4	-0.680823788779378
1109	5	-0.9548737329569335
1109	6	-1.0936233342815314
1109	7	-0.7934443244907214
1109	8	-1.246462545352081
1109	9	-0.8873034954782781
1109	10	-1.0591303784935917
1109	11	-1.1394978003839498
1109	12	-1.052960033644048
1109	13	-1.0447634190192758
1109	14	-0.5284723087509064
1109	15	-1.2057678038136572
1109	16	-1.2135732218719468
1109	17	-1.2637939970934013
1109	18	-1.8932247142372205
1109	19	-2.12199867604685
1109	20	-1.740939848831566
1109	21	-1.4994222987367465
1109	22	-1.5902538219204558
1109	23	-1.1237406178199407
1109	24	-2.0019018122168504
1109	25	-1.072138145952735
1110	1	0.06115170758554456
1110	2	0.19849647468019782
1110	3	0.029807031712998366
1110	4	0.2999584463625595
1110	5	0.1552347485419432
1110	6	0.07129033969505372
1110	7	0.08017057926748095
1110	8	0.017948334423266488
1110	9	0.2878437709631214
1110	10	0.2657489509886233
1110	11	0.12504353971328896
1110	12	0.1048698319157424
1110	13	0.1668042418703024
1110	14	-2.44644091602693
1110	15	-0.7121679476458596
1110	16	-0.2035029160079631
1110	17	-0.2472697973825433
1110	18	-2.779169114063464
1110	19	-0.5356875119470459
1110	20	-1.9266553366531483
1110	21	-0.4199200983954867
1110	22	-1.8628454750485446
1110	23	-0.6374525135776778
1110	24	0.0727306337781144
1110	25	-0.06360488232697037
1111	1	-0.27523647541296015
1111	2	-1.4585455493399344
1111	3	-0.45683957626376426
1111	4	-0.08421221946859703
1111	5	-0.1835579172361932
1111	6	-0.08446477007156261
1111	7	-0.11658228154256278
1111	8	-0.036039706483407485
1111	9	-1.6534578282269194
1111	10	-0.3765767310895214
1111	11	-0.17817542238657325
1111	12	-0.20578816515588017
1111	13	-0.05863479400765925
1111	14	0.737569330125865
1111	15	0.19589674899194165
1111	16	0.20712150643248606
1111	17	-0.16465890198428923
1111	18	-0.016967980953644712
1111	19	-0.2029180885584836
1111	20	0.20953538582896414
1111	21	-0.6391366982640463
1111	22	-0.28308952772854923
1111	23	-0.08121582760673363
1111	24	-0.5759561042652308
1111	25	-1.9425098022338005
1112	1	0.10711409387942084
1112	2	0.38251902058119924
1112	3	-0.07494211723504662
1112	4	0.35050721210469504
1112	5	0.16118746167697395
1112	6	0.053394995832853454
1112	7	-0.057883999671076936
1112	8	0.03557859652867279
1112	9	-0.4813927935773066
1112	10	0.3604026705042216
1112	11	0.08224725271810485
1112	12	-0.0877562446882365
1112	13	0.02044376788551716
1112	14	0.4702248205283084
1112	15	0.09013593835968692
1112	16	0.145448848413416
1112	17	-3.725370323825615
1112	18	-1.0057535588799595
1112	19	-1.2504223371791099
1112	20	-0.1600900521320625
1112	21	-0.5308498377782698
1112	22	-1.7374078576219745
1112	23	-1.6250234345740182
1112	24	-0.44011141469929527
1112	25	-0.03595359970331202
1113	1	-0.2507058639405386
1113	2	-0.07174893368675583
1113	3	0.006198963906033583
1113	4	-0.47197956038208133
1113	5	-0.15884435835484112
1113	6	-0.3071148646770041
1113	7	-0.3695390113260828
1113	8	-0.2562314452813723
1113	9	0.20080833862382264
1113	10	0.3021720122735658
1113	11	-0.08337971290885804
1113	12	-0.11787427480626178
1113	13	-0.5435884961467465
1113	14	-1.4963900460865602
1113	15	-1.7410865428627957
1113	16	-0.5755930726285072
1113	17	-0.3195446679999563
1113	18	-0.6980982512246499
1113	19	-0.2004352871920574
1113	20	0.19598905394704502
1113	21	-1.6986542555826887
1113	22	-2.1555693257834463
1113	23	-0.7757856353362129
1113	24	-2.463685988273866
1113	25	-0.7338094475591603
1114	1	0.17918689287529688
1114	2	0.27825149323674836
1114	3	0.07824657441518568
1114	4	0.3788298865812152
1114	5	0.10318634598355203
1114	6	0.09587103830889987
1114	7	0.10335561541469218
1114	8	0.09288738460669066
1114	9	0.4483513938591841
1114	10	0.19544540554695544
1114	11	0.07466950283266272
1114	12	0.16083419620989758
1114	13	0.056919969746331844
1114	14	0.8252434486238585
1114	15	0.2259656357278459
1114	16	0.21284071580527808
1114	17	-0.526871529172972
1114	18	-0.26759285918079456
1114	19	-0.6417866708511326
1114	20	-1.744788117599363
1114	21	-0.7694637879306825
1114	22	-1.5634264259482364
1114	23	-3.042010863099904
1114	24	-2.1563437578547138
1114	25	-1.586245473072104
1115	1	0.4213537472711941
1115	2	0.409932809341619
1115	3	0.35936101382619157
1115	4	0.5282568615501049
1115	5	0.5421760676032363
1115	6	0.29261848592916095
1115	7	0.36433318464087705
1115	8	0.44610321091862204
1115	9	0.5857764046392688
1115	10	0.7645866549874611
1115	11	0.44501541648377696
1115	12	0.32808126591236686
1115	13	0.2935077409494866
1115	14	0.9896669559305451
1115	15	0.6398370223500436
1115	16	0.5161550794687741
1115	17	0.5215632681204454
1115	18	0.7025321242186919
1115	19	0.0773155571423274
1115	20	0.6532045732573266
1115	21	-0.3868978490631889
1115	22	0.6255790354147877
1115	23	0.6697854187136443
1115	24	0.7436585375245244
1115	25	0.7456223425958228
1116	1	0.6483529666251435
1116	2	0.7184429478293275
1116	3	0.556716939081543
1116	4	0.8291036790573258
1116	5	0.6850716613602614
1116	6	0.4882919101818217
1116	7	0.4391041466321397
1116	8	0.43727824137899773
1116	9	0.5882557466191756
1116	10	0.5681314038037328
1116	11	0.3727227842895289
1116	12	0.3860380144941544
1116	13	-0.08934360354569293
1116	14	1.001799317399609
1116	15	0.5874600162773689
1116	16	0.6385780091224831
1116	17	0.7727270886553583
1116	18	0.7008789765197037
1116	19	0.8445609815485042
1116	20	0.6810957787797918
1116	21	0.2015848592683014
1116	22	0.28211999318440917
1116	23	-0.7241709216210452
1116	24	-0.19233455938819333
1116	25	-0.6612714663453758
1201	1	0.39209283419899066
1201	2	0.5761950642221052
1201	3	0.5389213949067511
1201	4	0.40382014939514477
1201	5	0.3648479835033456
1201	6	0.4151390467898395
1201	7	0.48615015778543125
1201	8	0.5582354768805615
1201	9	0.7742440385528511
1201	10	1.094079565952022
1201	11	0.7187955655489233
1201	12	0.72674801552931
1201	13	0.7807647097946528
1201	14	1.3698213953181337
1201	15	0.9870085056820059
1201	16	0.7479672391609
1201	17	0.7246700186954058
1201	18	0.7196108843341023
1201	19	0.9614137562451788
1201	20	1.0378817010048351
1201	21	0.9978897719522095
1201	22	1.0938504977755201
1201	23	0.6516660415884061
1201	24	1.0379014381203078
1201	25	1.2998124704619158
1202	1	0.26441841698300195
1202	2	0.6223227938422191
1202	3	0.4072081158445542
1202	4	0.2859582886338359
1202	5	0.25804915705168696
1202	6	0.2635975703476987
1202	7	0.368543847574621
1202	8	0.3601837380483133
1202	9	0.8871028371859779
1202	10	0.02728766489850898
1202	11	0.34965812905091553
1202	12	0.4543044758111492
1202	13	0.39947339858800746
1202	14	1.038140479150286
1202	15	0.7881295941600328
1202	16	0.6956672655463265
1202	17	-1.3216361329167015
1202	18	0.20777221040789584
1202	19	0.6114554243269055
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2210	20	0.7447898315842423
2210	21	0.8366404104033833
2210	22	0.9178396710656855
2210	23	1.2059203483933771
2210	24	0.8905490403287857
2210	25	0.9049305083814758
2211	1	0.12994432809755452
2211	2	-0.5083443667234704
2211	3	-0.03517484615104749
2211	4	0.4347157609039911
2211	5	0.28207948648359604
2211	6	0.25059492257015137
2211	7	0.2249679052448883
2211	8	0.2396730061390926
2211	9	0.6126367237618169
2211	10	0.7778629879645393
2211	11	0.38900436801614546
2211	12	0.17107165470905983
2211	13	0.2579883374585563
2211	14	-1.0594669454623897
2211	15	0.00398866662522579
2211	16	0.2269104736472798
2211	17	0.3048552499942687
2211	18	0.18385905599956232
2211	19	0.577088493890443
2211	20	0.5473251437172948
2211	21	0.5928490216857156
2211	22	7.737380329260191E-4
2211	23	0.585743701282091
2211	24	-0.13872447292089163
2211	25	0.31717752513600317
2212	1	-0.06207682256496838
2212	2	-1.1816835611594385
2212	3	-0.3699638100065741
2212	4	0.1291682990384473
2212	5	0.14309399669403433
2212	6	-0.01071343905651645
2212	7	0.013984921172038867
2212	8	0.024554501619732605
2212	9	-2.4368276851802975
2212	10	-0.13421825750518934
2212	11	-0.027138909811668555
2212	12	-0.11631059471842775
2212	13	0.009744228926550136
2212	14	0.5454337819715533
2212	15	0.17958224345787954
2212	16	0.20856155500725748
2212	17	0.27391883959326435
2212	18	0.05914648947475598
2212	19	-0.6158608609564808
2212	20	0.12754194843838462
2212	21	0.08730724596452433
2212	22	-1.8833419392277975
2212	23	-0.48570592682500546
2212	24	0.10534001109627411
2212	25	-0.6157359812064058
2213	1	0.1880685173817227
2213	2	0.398834548886736
2213	3	0.18243599359851714
2213	4	0.4306308184148122
2213	5	0.4051553111402484
2213	6	0.19181387796530913
2213	7	0.06975710748709929
2213	8	0.15393521292858736
2213	9	0.6482024931786796
2213	10	0.7142651924766525
2213	11	-0.01849483370258298
2213	12	0.040430423548393435
2213	13	0.04310529854038947
2213	14	-1.7935800334513985
2213	15	-0.47318489136414144
2213	16	-0.14611514006980997
2213	17	-2.1851430440895925
2213	18	-0.8898235118091904
2213	19	-0.6068867873987642
2213	20	0.16612225130754865
2213	21	-0.2770466098284956
2213	22	0.3070524265968402
2213	23	0.7539560634312963
2213	24	0.5640838827099941
2213	25	-0.9256085449369422
2214	1	0.3377528798243645
2214	2	0.24261778008580137
2214	3	0.18938459601062974
2214	4	0.4565082746084812
2214	5	0.5646488233822708
2214	6	0.28199946490605043
2214	7	0.2623178538201003
2214	8	0.26255193890884243
2214	9	0.636870296141467
2214	10	0.587724744187085
2214	11	-0.01564346610015832
2214	12	-0.007104316371673747
2214	13	0.15271443072227697
2214	14	0.9400138701820211
2214	15	0.4477698502352254
2214	16	0.4562943335780929
2214	17	0.43399010113144243
2214	18	0.40347627591515334
2214	19	-0.8311851282076219
2214	20	0.18667364580344525
2214	21	0.4221647944326037
2214	22	0.6319678880617374
2214	23	1.1703702058518104
2214	24	0.911759136552341
2214	25	0.9946836580588894
2215	1	-0.00457470325933329
2215	2	0.39655764059122234
2215	3	0.3859306983896609
2215	4	0.5311650662276435
2215	5	0.5075810301058681
2215	6	0.2782666447583324
2215	7	0.22867205711206395
2215	8	0.248578939175772
2215	9	0.3985738195684476
2215	10	0.6663086605986024
2215	11	0.40335799569596276
2215	12	0.2635895655305802
2215	13	0.22140233024020972
2215	14	0.9944125911416464
2215	15	0.4884701353902656
2215	16	0.42607456167159086
2215	17	0.5697718747228109
2215	18	0.4235297423125104
2215	19	-0.24925223979370514
2215	20	-1.3971748049022636
2215	21	-1.5815829893104123
2215	22	-0.0020519152433280397
2215	23	0.6238834928442643
2215	24	0.7232345484364089
2215	25	0.06030857347223417
2216	1	0.2846486991918056
2216	2	0.07666729343317513
2216	3	0.1769440281696184
2216	4	0.5334227876128637
2216	5	0.5795532180056995
2216	6	0.2132341278942107
2216	7	0.11337176788099157
2216	8	0.14788998198820025
2216	9	0.34650968802887405
2216	10	-1.3535548054902904
2216	11	-0.3563111710255029
2216	12	0.009668621255151777
2216	13	0.09594258319914672
2216	14	-0.460536496344119
2216	15	-0.3661896633362858
2216	16	0.11981285888853677
2216	17	0.4683882495625866
2216	18	0.2514948511462336
2216	19	-1.0543411549868154
2216	20	-0.6968309175766336
2216	21	-0.7077110424172517
2216	22	-0.49480279464654586
2216	23	-4.275578366610429
2216	24	-1.924264675704407
2216	25	-0.9124999271755598
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	27.0
5	40.0
6	59.0
7	207.0
8	170.0
9	506.0
10	1186.0
11	2629.0
12	4975.0
13	8223.0
14	11769.0
15	15465.0
16	18005.0
17	19698.0
18	20676.0
19	21312.0
20	21382.0
21	22173.0
22	23400.0
23	24513.0
24	26493.0
25	29718.0
26	33851.0
27	39415.0
28	46899.0
29	57827.0
30	71969.0
31	90459.0
32	120918.0
33	164259.0
34	225605.0
35	315968.0
36	457451.0
37	698460.0
38	1057164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09117169277878	21.413903462591087	13.736003149727974	22.75892169490216
2	15.989667657022947	21.19066726794463	40.74498777593141	22.074677299101012
3	18.234553955898765	26.474516166574247	30.274670883736217	25.016258993790775
4	12.572790488271366	17.930696225410617	38.42633975028864	31.070173536029376
5	13.52161071678584	37.728209892299716	35.180776743487975	13.569402647426465
6	30.348526643622296	38.78145042609261	17.86897566651836	13.001047263766727
7	25.67149573537678	33.22438700233855	23.68516235303266	17.418954909252005
8	23.242255160373805	37.23090404956697	21.345702255845	18.18113853421422
9	24.232677653002348	17.46730977725819	22.497998837230636	35.802013732508826
10	14.937697416553858	30.19879107688129	33.806886634160016	21.056624872404836
11	31.331361918843438	26.06475548984174	23.873309238267346	18.73057335304748
12	20.731905316662946	27.938566169776735	31.49876039902694	19.83076811453338
13	25.874250910024866	24.686194072557747	27.157212101099148	22.28234291631824
14	20.761897250347527	23.901387807146403	28.32681001534705	27.009904927159024
15	22.528581017225974	31.238099515368038	24.530340775931453	21.702978691474538
16	21.77332000052572	31.566115684376083	25.832168720619997	20.828395594478202
17	19.901406669014126	30.486115940806762	28.59685765245577	21.01561973772334
18	19.87310396646628	30.174213012936047	29.581491174890456	20.371191845707216
19	20.68120152120404	29.076928131596745	29.854122117967236	20.387748229231978
20	22.174195765232856	28.623077797110657	29.025499776173653	20.177226661482837
21	22.614826187850507	29.000778631120493	27.905465859051343	20.478929321977656
22	21.521891093926307	28.54739604396591	29.535706355058455	20.395006507049327
23	21.730198509800637	28.898165329276672	28.765507850885875	20.606128310036816
24	21.36637068311689	28.170241195105454	29.118702819920976	21.344685301856675
25	21.975060367656912	28.528887270132213	28.509456708901414	20.986595653309458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2418.0
1	2418.0
2	2877.0
3	3336.0
4	3336.0
5	3336.0
6	5313.0
7	7290.0
8	7290.0
9	7290.0
10	11719.5
11	16149.0
12	16149.0
13	16149.0
14	26453.0
15	36757.0
16	36757.0
17	36757.0
18	57957.5
19	79158.0
20	79158.0
21	79158.0
22	112097.5
23	145037.0
24	145037.0
25	145037.0
26	185894.5
27	226752.0
28	226752.0
29	226752.0
30	270538.0
31	314324.0
32	314324.0
33	314324.0
34	350632.0
35	386940.0
36	386940.0
37	386940.0
38	412090.5
39	437241.0
40	437241.0
41	437241.0
42	437543.5
43	437846.0
44	437846.0
45	437846.0
46	422206.0
47	406566.0
48	406566.0
49	406566.0
50	388561.5
51	370557.0
52	370557.0
53	370557.0
54	335089.0
55	299621.0
56	299621.0
57	299621.0
58	258028.0
59	216435.0
60	216435.0
61	216435.0
62	177037.5
63	137640.0
64	137640.0
65	137640.0
66	105928.5
67	74217.0
68	74217.0
69	74217.0
70	53722.5
71	33228.0
72	33228.0
73	33228.0
74	23397.0
75	13566.0
76	13566.0
77	13566.0
78	9315.5
79	5065.0
80	5065.0
81	5065.0
82	3455.5
83	1846.0
84	1846.0
85	1846.0
86	1240.0
87	634.0
88	634.0
89	634.0
90	417.5
91	201.0
92	201.0
93	201.0
94	119.0
95	37.0
96	37.0
97	37.0
98	28.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08327673417228756
2	0.01806793049102886
3	0.02890868878564618
4	0.03572522619817071
5	0.044813942748203404
6	0.013852080043122127
7	0.017082407009700016
8	0.03438381923747311
9	0.03432906793295484
10	0.01929983484268992
11	0.01864281918847069
12	0.013578323520530781
13	0.021435135718902425
14	0.037367765333718786
15	0.03405531141036349
16	0.02017585571498223
17	0.01935458614720819
18	0.025048721817108193
19	0.013988958304417801
20	0.013578323520530781
21	0.00867808176614568
22	0.01883444875428463
23	0.016918153096145208
24	0.01782154962069665
25	0.1092288525139472
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3652881.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.27992717404207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.85756366929385	67.99203758547046
2	9.650501772496648	15.108811518880033
3	1.856653986223657	4.360162164869484
4	0.6168329087249558	1.9314254069416832
5	0.29764092717432095	1.1649655051610104
6	0.16932361695010328	0.7952784242219689
7	0.11220548443244645	0.6148406004930014
8	0.08184901205952941	0.5125707762629802
9	0.0556764007395252	0.39225101356825337
>10	0.27768309966747	3.744665787016114
>50	0.014720558790088434	0.8005978891780174
>100	0.008154652993567957	1.1356720560560738
>500	7.023002761508203E-4	0.4031665985113316
>1k	4.213801522335275E-4	0.7028958105767306
>5k	7.02300253722532E-5	0.3406588627929037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6716	0.18385488057234825	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5677	0.15541157787510734	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4678	0.12806330126823184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.4751304518269275E-5	0.0
5	0.0	0.0	0.0	5.4751304518269275E-5	0.0
6	0.0	0.0	0.0	8.212695677740392E-5	0.0
7	0.0	0.0	0.0	8.212695677740392E-5	0.0
8	2.7375652259134638E-5	0.0	0.0	1.0950260903653855E-4	0.0
9	2.7375652259134638E-5	0.0	0.0	1.368782612956732E-4	0.0
10	2.7375652259134638E-5	0.0	0.0	1.368782612956732E-4	0.0
11	2.7375652259134638E-5	0.0	0.0	1.368782612956732E-4	0.0
12	2.7375652259134638E-5	0.0	0.0	1.368782612956732E-4	0.0
13	2.7375652259134638E-5	0.0	0.0	1.6425391355480784E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3420	0.0	10.620474	1
GTGGTAT	1650	0.0	10.142275	1
TGGTATC	1565	0.0	10.078798	2
CGAGACT	125	2.5660847E-6	9.881945	4
TTACCCG	80	0.0045105848	9.501869	5
GTCCCGA	85	0.0073851496	8.949065	1
GTCCTAG	320	0.0	8.914108	1
GTATTAA	630	0.0	8.9046755	1
CCAGGAC	560	0.0	8.823164	3
ATACCGT	110	7.1604614E-4	8.63487	6
ACACCGT	100	0.0029109016	8.54852	6
GTTCTAG	445	0.0	8.54686	1
CTATACT	480	0.0	8.512091	4
CTTATAC	385	0.0	8.391262	3
CAGGACT	435	0.0	8.300484	4
TTACACT	585	0.0	8.283681	4
GTTTAGG	345	0.0	8.268158	1
AAGGCCG	105	0.0045068758	8.14446	5
GTATCAA	5985	0.0	8.134155	1
GTCCTGG	705	0.0	8.09224	1
>>END_MODULE