FastQCFastQC Report
Wed 25 May 2016
SRR1295264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652881
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167790.4593360692560201No Hit
GGTATCAACGCAGAGTACTTTTTTT142710.3906779333901105No Hit
TATCAACGCAGAGTACTTTTTTTTT102770.2813395782671267No Hit
GTGGTATCAACGCAGAGTACATGGG76110.20835608934427371No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71070.1945587606056699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2850.012.6745043
TGAACCG751.4704963E-512.6745045
CAGCGTC3100.011.9590082
GTGGTAT19600.011.5572611
GGTATCA49750.011.4021621
GCAGCGT3300.010.9598271
TGGTATC20300.010.9575142
GTACTAG2650.010.4156451
AAGGCCG1151.019831E-59.9191785
GTCTAGG2550.09.7043671
GTCTTAG3400.09.5177441
GGACCGT800.00452958339.4970296
CCCCTAT1752.5933332E-89.2458091
TACACTG7400.09.1205045
GCGTCAG4150.08.7041774
TAGGACC2451.546141E-108.5358914
CTATACT5050.08.4705854
CTTAGAC3150.08.449673
TAGACTG5750.08.2659815
GTCTCGC2658.54925E-118.2606841
Produced by FastQC (version 0.11.5)