FastQCFastQC Report
Wed 25 May 2016
SRR1295263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217744
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG52440.16297132400837358No Hit
GTATCAACGCAGAGTACTTTTTTTT41890.13018437762606347No Hit
GGTATCAACGCAGAGTACTTTTTTT35210.10942449119631643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGA905.390393E-712.670655
GTGGTAT14650.011.552941
TAGACAG3850.011.3542175
TGGTATC14000.010.8600492
CTAGTAC909.49262E-510.5587093
GGTATCA30350.010.4013461
CAGGACG951.6398556E-410.0031434
TCTAGAC3500.09.7743483
CGGTCAA800.0045227629.49870210
ACCGCGT850.00744670868.93995510
CGCGTAT850.0074486088.93967612
GTCTAGC1502.6713678E-68.8745621
AGACAGT6250.08.6626826
CTAGACA4100.08.5758664
CTACCGC1000.0029097518.54883110
CCGCGTA1000.00291057028.54856511
TAGAACA4800.08.5130924
GTCCTAG3600.08.4519631
TTTAGAC3050.08.4123493
TTGGACT3400.08.3849884
Produced by FastQC (version 0.11.5)