FastQCFastQC Report
Wed 25 May 2016
SRR1295263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217744
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129120.40127493051032026No Hit
GGTATCAACGCAGAGTACTTTTTTT109670.34082885400454477No Hit
TATCAACGCAGAGTACTTTTTTTTT77900.24209508276606218No Hit
GTGGTATCAACGCAGAGTACATGGG58780.18267456951205566No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52550.1633131784256299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC400.00525744114.2594972
AGTGTCG500.001504230213.2952138
GTGGTAT15800.011.9287691
GGTATCA39050.011.5299551
TGGTATC16400.010.9554662
AGCGTCA3300.010.9466853
GCAGCGT3350.010.5134311
GTACTAG1502.3734174E-810.1535111
CAGCGTC3800.09.50633052
AGAACCG1201.6888915E-59.5060355
TAGACAG3600.09.2419795
CTAGCAC2106.6029315E-109.0536483
TGTACCG1054.4663332E-49.0533675
GTACCGT950.00183028848.99690156
AATCCGA950.00183054728.99676111
GACTGTG5600.08.9878367
GTATTAC3400.08.9589811
GAACCGT850.00745940678.9380986
CTAGACA3050.08.7268514
TAGTACT3400.08.6672684
Produced by FastQC (version 0.11.5)