FastQCFastQC Report
Wed 25 May 2016
SRR1295262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007326
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.011.6804671
ACGACCG1002.3949968E-510.4526365
GTATCAA80850.09.6898981
GTGGTAT17300.08.738221
CGTAGCA1752.6212547E-78.6875942
ACCGACC1554.221358E-68.5793788
ACCGCGT1252.7502517E-48.3587668
ACGCACG1150.00111340428.25941511
CGTATAC1502.5809242E-58.235413
GTATTAT10300.08.2153251
GTCTTAA7650.08.2026551
ACCGTCC1659.776068E-68.0594168
TGGTATC17100.07.94616372
GTCTCGC4800.07.923021
TACGCCG1200.00168795787.9186635
GTACCGT1803.6594502E-67.91532856
GTCTAAT6200.07.8207871
GTATTAA12200.07.79313471
TAGACCG1100.00684246477.7746885
ATTATAC10300.07.74952653
Produced by FastQC (version 0.11.5)