##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7007326 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.747739437268937 31.0 30.0 31.0 25.0 34.0 2 29.737307640603564 31.0 30.0 33.0 25.0 34.0 3 30.137499240081024 31.0 30.0 34.0 26.0 34.0 4 33.554733146424184 35.0 33.0 37.0 28.0 37.0 5 33.73921179063169 35.0 35.0 37.0 28.0 37.0 6 33.89718902759769 35.0 35.0 37.0 28.0 37.0 7 33.79699973998641 35.0 35.0 37.0 28.0 37.0 8 33.799122803762806 35.0 35.0 37.0 28.0 37.0 9 34.8021121038182 38.0 34.0 39.0 27.0 39.0 10 34.66949575344432 37.0 34.0 39.0 27.0 39.0 11 35.08493411038676 38.0 35.0 39.0 27.0 39.0 12 35.08612843187259 38.0 35.0 39.0 27.0 39.0 13 34.99056687244178 38.0 34.0 39.0 27.0 39.0 14 35.06954778470418 38.0 33.0 40.0 24.0 41.0 15 35.699529035754864 39.0 34.0 40.0 27.0 41.0 16 35.81346579280028 39.0 34.0 40.0 27.0 41.0 17 35.60984032996324 39.0 34.0 40.0 27.0 41.0 18 35.57665177843874 38.0 34.0 40.0 27.0 41.0 19 35.6257415453484 39.0 34.0 40.0 26.0 41.0 20 35.589772903387114 39.0 34.0 40.0 26.0 41.0 21 35.571373730863954 39.0 34.0 40.0 26.0 41.0 22 35.300329540826276 39.0 34.0 40.0 25.0 41.0 23 34.96000899629902 38.0 33.0 40.0 24.0 41.0 24 35.29330089680429 38.0 34.0 40.0 25.0 41.0 25 33.81588997001138 38.0 31.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21210914425590133 1101 2 0.45430311574969906 1101 3 0.3088822794200361 1101 4 0.3652698563385428 1101 5 0.1823084435857325 1101 6 0.2012259004194732 1101 7 0.1857449415840975 1101 8 0.21068245131833407 1101 9 0.08486923216842968 1101 10 0.35254981203885905 1101 11 0.34193107779089615 1101 12 0.35815805029812964 1101 13 0.39342100141652736 1101 14 0.9472832748038016 1101 15 0.6916286519232031 1101 16 0.4688165767539587 1101 17 0.7835888300614755 1101 18 0.7743336000248746 1101 19 0.7867635484176816 1101 20 0.833949087658354 1101 21 0.9053106623004297 1101 22 0.6061335298433832 1101 23 -1.6256678578552766 1101 24 0.033308841323481886 1101 25 0.44107257452385795 1102 1 0.18737275483142923 1102 2 0.2796758135688293 1102 3 0.19536132254029326 1102 4 0.46282330571634844 1102 5 0.1546684583756388 1102 6 0.14228360362488246 1102 7 0.09364466137291316 1102 8 0.17442199332394637 1102 9 0.3338638114786221 1102 10 0.8635095247543134 1102 11 0.588210436402683 1102 12 0.5212228913545971 1102 13 0.46806874043567603 1102 14 1.003400412121458 1102 15 0.7024712159095685 1102 16 0.46852463427138247 1102 17 0.8295447264307967 1102 18 0.7863945159808026 1102 19 0.7741738854638527 1102 20 0.945674196276606 1102 21 0.8699977681683535 1102 22 0.8006175632176777 1102 23 0.7841002851770043 1102 24 0.8051940108441968 1102 25 0.6792948047379213 1103 1 -0.4360293413806673 1103 2 -1.568236735224076 1103 3 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1.0749411561488031 2210 11 0.697480361844903 2210 12 0.5292467369447209 2210 13 0.5397080021655967 2210 14 -1.3196928699768975 2210 15 -0.04267917946414457 2210 16 0.2977531715412667 2210 17 0.8121913957253639 2210 18 0.7566513910339197 2210 19 0.8567549066534923 2210 20 0.9797845451544447 2210 21 1.0496786224444534 2210 22 1.3039956720306236 2210 23 1.6220662733866718 2210 24 1.277133160752662 2210 25 1.2906428682916058 2211 1 0.11748589848501467 2211 2 -0.560076091258594 2211 3 -0.031358059191784804 2211 4 0.2766974564086979 2211 5 0.3349088228756898 2211 6 0.25051625537667377 2211 7 0.271730992969637 2211 8 0.3966556929818381 2211 9 0.8629759176819292 2211 10 0.875027264224677 2211 11 0.5242217612238704 2211 12 0.30750519632979234 2211 13 0.1856562859869797 2211 14 -0.8956410044006375 2211 15 -0.028813212594073434 2211 16 0.21137413560317242 2211 17 0.3819491431708073 2211 18 0.30581719174613653 2211 19 0.6438994399504097 2211 20 0.7077213075725695 2211 21 0.6881127022576479 2211 22 -0.0052481385035036965 2211 23 0.522410862705371 2211 24 -0.20803065049906877 2211 25 0.42621371350154647 2212 1 0.07598225313738993 2212 2 -1.086363676942387 2212 3 -0.249873356903894 2212 4 0.23458599579917916 2212 5 0.22706285874398446 2212 6 0.15502936031739267 2212 7 0.20204638540472786 2212 8 0.2506414053250552 2212 9 -2.163839344040113 2212 10 0.08189975301836228 2212 11 0.19132443248165032 2212 12 0.13897741723893375 2212 13 0.1548739829090593 2212 14 0.804043618987798 2212 15 0.47790950272715094 2212 16 0.4692436990578699 2212 17 0.5827419507626672 2212 18 0.27060484031690635 2212 19 -0.5440704159029934 2212 20 0.48265926592564057 2212 21 0.45472805667481 2212 22 -1.5977727935088666 2212 23 -0.13842691533483276 2212 24 0.49745930802471605 2212 25 -0.14293141951581134 2213 1 0.28775516916880406 2213 2 0.585917000821361 2213 3 0.2898266855479612 2213 4 0.4785297210541586 2213 5 0.4695625201394833 2213 6 0.36939059788041106 2213 7 0.38063218286483647 2213 8 0.3391031395117423 2213 9 0.8124395063591052 2213 10 0.778161451184161 2213 11 0.15470369088764357 2213 12 0.21286942370837636 2213 13 0.23183144780836074 2213 14 -1.5224635643674063 2213 15 -0.253593585498777 2213 16 0.12914691241367393 2213 17 -2.0017515474157292 2213 18 -0.7532507146740528 2213 19 -0.3213085506624793 2213 20 0.4236022637168517 2213 21 0.039049674621630004 2213 22 0.5599531248512264 2213 23 1.0728524303422091 2213 24 0.8172900643040748 2213 25 -0.7333351386067548 2214 1 0.38863396921094306 2214 2 0.2609290739565786 2214 3 0.251454586917788 2214 4 0.5838118218220671 2214 5 0.5659975589276698 2214 6 0.46949946431785605 2214 7 0.4592761034768671 2214 8 0.3851854235721177 2214 9 0.7761974667744198 2214 10 0.8184226283502767 2214 11 0.2192214442046705 2214 12 0.13555583474263244 2214 13 0.2606005125856825 2214 14 1.1454005478746865 2214 15 0.7604852609509649 2214 16 0.6782120608778044 2214 17 0.5851245345088785 2214 18 0.47894244467657643 2214 19 -0.5736783774244998 2214 20 0.3739185469826367 2214 21 0.7167916498406157 2214 22 0.9291009638805718 2214 23 1.4815331789481903 2214 24 1.0837896309862813 2214 25 1.1339669463597701 2215 1 -0.026654472699764398 2215 2 0.44919769356541295 2215 3 0.33147720256655333 2215 4 0.49873048972776246 2215 5 0.48709582482362634 2215 6 0.4049467075409794 2215 7 0.3654781585270044 2215 8 0.3188071922689133 2215 9 0.5156696014696323 2215 10 0.8304637299348983 2215 11 0.4783979139199417 2215 12 0.38497647606155283 2215 13 0.22395211128537085 2215 14 1.155618168206189 2215 15 0.6166469849547838 2215 16 0.6339451001960654 2215 17 0.7449458426577138 2215 18 0.587985161394478 2215 19 -0.11770088569839743 2215 20 -1.4106070765445082 2215 21 -1.7922885680576073 2215 22 -0.07016515511609356 2215 23 0.6917947676858773 2215 24 0.8607507026514369 2215 25 0.13405801857634714 2216 1 0.282327986232751 2216 2 -0.05516959875523142 2216 3 0.09390711054383516 2216 4 0.4268229326681592 2216 5 0.556950723080682 2216 6 0.2636975914351538 2216 7 0.3755718286231442 2216 8 0.2702031691805189 2216 9 0.5782692031675509 2216 10 -1.4399413640877086 2216 11 -0.27174019345409306 2216 12 0.016803227917122854 2216 13 0.20653250290022385 2216 14 -0.2651121081272265 2216 15 -0.17351608435857457 2216 16 0.2438422020004296 2216 17 0.5551161576346644 2216 18 0.4218159884122912 2216 19 -1.1821999960453908 2216 20 -0.7725355120047013 2216 21 -0.6035814722633717 2216 22 -0.5398617377177075 2216 23 -4.148783880223036 2216 24 -1.795956178146227 2216 25 -0.8585899364084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 43.0 4 82.0 5 119.0 6 153.0 7 536.0 8 299.0 9 804.0 10 2213.0 11 5287.0 12 10591.0 13 17572.0 14 25387.0 15 33226.0 16 39199.0 17 43570.0 18 47131.0 19 49671.0 20 52322.0 21 54757.0 22 59086.0 23 63620.0 24 70162.0 25 77601.0 26 88274.0 27 101927.0 28 119094.0 29 144929.0 30 175883.0 31 215654.0 32 278070.0 33 361946.0 34 476361.0 35 631977.0 36 854881.0 37 1194070.0 38 1710825.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.602553641482125 20.642328085100246 12.930157704079292 23.824960569338334 2 15.896374456248369 21.23460381424034 40.09017470278146 22.778847026729828 3 17.591351180357805 27.0635062933573 29.87651680959932 25.46862571668558 4 12.621720841354787 18.773487059676743 37.69154940535972 30.91324269360875 5 13.32122981320878 39.125842390865934 34.19898821006156 13.353939585863724 6 30.540060341650555 38.83943520123732 17.69398007966194 12.926524377450185 7 26.510952522535163 32.6775032619266 23.565319678798897 17.246224536739334 8 24.278119878805203 36.234877953616426 21.41639676035332 18.070605407225063 9 25.465644138321714 16.610874742561958 21.48210361251281 36.441377506603516 10 15.852283335626726 28.909114215028257 33.34066553348251 21.897936915862502 11 32.25933628615433 25.109303384515247 24.17453998369906 18.45682034563136 12 21.76736356333645 26.760934887101367 31.111681407137887 20.360020142424297 13 26.884634111630117 23.59138398326689 26.69311346452814 22.830868440574857 14 21.88788469928754 22.28326293980725 27.75482722938848 28.074025131516734 15 23.05216710765742 29.96972596329141 24.1052341847924 22.872872744258768 16 22.910276246027646 30.014289990454785 25.14490187354322 21.930531889974354 17 21.1961782999447 29.214203107821966 27.777340037609495 21.812278554623834 18 21.266782170069792 29.245410281420707 28.29237159358581 21.195435954923695 19 22.03783097967944 27.819884993798937 28.735335724502388 21.406948302019238 20 23.160612628377276 27.667646036620397 27.933967104873464 21.237774230128863 21 23.60575694820088 27.918786724879364 27.02600017983327 21.449456147086487 22 22.72281219414124 27.45480266672795 28.32958101391558 21.49280412521523 23 22.747185614554443 27.83864332847557 27.813436255079598 21.600734801890386 24 22.367882413192433 27.188580687632065 28.00495749616469 22.43857940301081 25 23.004753188090444 27.365497177531168 27.518003212412832 22.111746421965556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3746.0 1 3746.0 2 4246.0 3 4746.0 4 4746.0 5 4746.0 6 7029.0 7 9312.0 8 9312.0 9 9312.0 10 15249.0 11 21186.0 12 21186.0 13 21186.0 14 36733.5 15 52281.0 16 52281.0 17 52281.0 18 85684.0 19 119087.0 20 119087.0 21 119087.0 22 175101.0 23 231115.0 24 231115.0 25 231115.0 26 304859.0 27 378603.0 28 378603.0 29 378603.0 30 460081.0 31 541559.0 32 541559.0 33 541559.0 34 616935.0 35 692311.0 36 692311.0 37 692311.0 38 755423.5 39 818536.0 40 818536.0 41 818536.0 42 830392.0 43 842248.0 44 842248.0 45 842248.0 46 824037.0 47 805826.0 48 805826.0 49 805826.0 50 775400.5 51 744975.0 52 744975.0 53 744975.0 54 686721.0 55 628467.0 56 628467.0 57 628467.0 58 549665.5 59 470864.0 60 470864.0 61 470864.0 62 393599.5 63 316335.0 64 316335.0 65 316335.0 66 247649.5 67 178964.0 68 178964.0 69 178964.0 70 133532.5 71 88101.0 72 88101.0 73 88101.0 74 63282.5 75 38464.0 76 38464.0 77 38464.0 78 26322.5 79 14181.0 80 14181.0 81 14181.0 82 9468.0 83 4755.0 84 4755.0 85 4755.0 86 3023.5 87 1292.0 88 1292.0 89 1292.0 90 790.0 91 288.0 92 288.0 93 288.0 94 171.0 95 54.0 96 54.0 97 54.0 98 42.0 99 30.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08269916370381512 2 0.019550967087873464 3 0.0308534239737098 4 0.03811725043190512 5 0.04713638269434018 6 0.017410350253434764 7 0.021363356007698227 8 0.03903058028126563 9 0.038930684828991834 10 0.02423178256584609 11 0.02277616311842777 12 0.017381808695642247 13 0.02590146369670827 14 0.04129963412577065 15 0.03773193940170616 16 0.024588552038252536 17 0.023518243621033188 18 0.029240825958432644 19 0.018109618419351405 20 0.01599754314270522 21 0.011930371157271689 22 0.020507109273922748 23 0.01935117618332585 24 0.019550967087873464 25 0.10850073194824958 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7007326.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.93141051319473 #Duplication Level Percentage of deduplicated Percentage of total 1 85.60235796215345 60.718959935109496 2 9.537293169822423 13.52987314026725 3 2.1917772261587793 4.66397550546419 4 0.8409727978672651 2.386055470238117 5 0.44878632695342496 1.591652359492111 6 0.2837643582913473 1.2076683712186096 7 0.1886894341401707 0.936880539874921 8 0.14167273862623428 0.8039237745620771 9 0.10572817105101882 0.6749503473266178 >10 0.5955581761465244 7.734576179501129 >50 0.041549269694768735 2.015455468584836 >100 0.02096032141947335 2.6536268633124322 >500 4.248149568600805E-4 0.21723828603429318 >1k 4.045501905374233E-4 0.605098116864563 >5k 6.068252776871788E-5 0.2600656421494954 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.4270778896258001E-5 0.0 0.0 0.0 6 0.0 1.4270778896258001E-5 0.0 0.0 0.0 7 0.0 1.4270778896258001E-5 0.0 0.0 0.0 8 0.0 1.4270778896258001E-5 1.4270778896258001E-5 1.4270778896258001E-5 0.0 9 0.0 1.4270778896258001E-5 1.4270778896258001E-5 2.8541557792516003E-5 0.0 10 0.0 2.8541557792516003E-5 1.4270778896258001E-5 2.8541557792516003E-5 0.0 11 0.0 2.8541557792516003E-5 1.4270778896258001E-5 2.8541557792516003E-5 0.0 12 0.0 2.8541557792516003E-5 1.4270778896258001E-5 2.8541557792516003E-5 0.0 13 0.0 4.2812336688774004E-5 1.4270778896258001E-5 2.8541557792516003E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4135 0.0 11.680467 1 ACGACCG 100 2.3949968E-5 10.452636 5 GTATCAA 8085 0.0 9.689898 1 GTGGTAT 1730 0.0 8.73822 1 CGTAGCA 175 2.6212547E-7 8.687594 2 ACCGACC 155 4.221358E-6 8.579378 8 ACCGCGT 125 2.7502517E-4 8.358766 8 ACGCACG 115 0.0011134042 8.259415 11 CGTATAC 150 2.5809242E-5 8.23541 3 GTATTAT 1030 0.0 8.215325 1 GTCTTAA 765 0.0 8.202655 1 ACCGTCC 165 9.776068E-6 8.059416 8 TGGTATC 1710 0.0 7.9461637 2 GTCTCGC 480 0.0 7.92302 1 TACGCCG 120 0.0016879578 7.918663 5 GTACCGT 180 3.6594502E-6 7.9153285 6 GTCTAAT 620 0.0 7.820787 1 GTATTAA 1220 0.0 7.7931347 1 TAGACCG 110 0.0068424647 7.774688 5 ATTATAC 1030 0.0 7.7495265 3 >>END_MODULE