FastQCFastQC Report
Wed 25 May 2016
SRR1295262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007326
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT192940.2753404080244019No Hit
GGTATCAACGCAGAGTACTTTTTTT135020.19268405665727556No Hit
TATCAACGCAGAGTACTTTTTTTTT122880.17535933107721832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54700.012.8411181
TTCGCGG855.3493393E-511.17225314
GTGGTAT18200.010.9297111
TGGTATC18550.09.994022
GTATCAA104350.09.339881
CTTCGCG1054.5134508E-49.04420513
AGCGTCA3050.09.0395693
TAGACAG8800.08.8587925
GCTCTAG5200.08.7856051
CGGGCGA1107.1771146E-48.63310318
GTGTTAG8900.08.341391
TTAGACA7950.08.2514144
TCTAGAC5650.08.2451243
TATACTG11850.08.1832385
GTTAGCC6650.08.1489713
CGGATAG1050.0044860828.1488545
ACCGCGT1401.04476625E-48.1397848
TCAGGAC7650.07.953683
TAGCACC5500.07.9513634
GTCCTAT5650.07.9174111
Produced by FastQC (version 0.11.5)