FastQCFastQC Report
Wed 25 May 2016
SRR1295261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282808
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA638194.9749455881160705No Hit
GCTTATGTACTCTGCGTTGATACCA372552.9041758392526393No Hit
CTTATGTACTCTGCGTTGATACCAC249541.9452638274784693No Hit
GAGTACATAAGCAGTGGTATCAACG157611.2286328117691814No Hit
TATGTACTCTGCGTTGATACCACTG147251.1478724797475537No Hit
CCCATGTACTCTGCGTTGATACCAC128831.0042812330450075No Hit
GCGTTGATACCACTGCTTATGTACT112850.8797107595213003No Hit
CATAAGCAGTGGTATCAACGCAGAG102130.7961440839159095No Hit
CTGCTTATGTACTCTGCGTTGATAC87770.6842021565191362No Hit
ACTCTGCGTTGATACCACTGCTTAT85830.6690790827621904No Hit
CATGTACTCTGCGTTGATACCACTG81670.6366502235720388No Hit
ACGCAGAGTACATAAGCAGTGGTAT80300.6259705271560514No Hit
GTATCAACGCAGAGTACATAAGCAG72710.5668034499317123No Hit
GTACATGGGAAGCAGTGGTATCAAC65860.5134049678517751No Hit
GTACATGATAAGCAGTGGTATCAAC64620.5037386732854799No Hit
TATCAACGCAGAGTACATAAGCAGT63860.49781417016420226No Hit
ATCATGTACTCTGCGTTGATACCAC63310.49352670080011973No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA62710.4888494614938479No Hit
GGTATCAACGCAGAGTACATAAGCA61250.47746817918191964No Hit
AAGCAGTGGTATCAACGCAGAGTAC49870.3887565403396299No Hit
GTGGTATCAACGCAGAGTACATAAG46810.36490261987764344No Hit
GTACTGGTTCACTATCGGTCAGTCA43770.3412046073925326No Hit
GTATCAACGCAGAGTACTTTTTTTT41250.3215602023061908No Hit
GTGGTATCAACGCAGAGTACATGGG39010.3040985088961091No Hit
GCAGTGGTATCAACGCAGAGTACAT38550.3005126254279674No Hit
ACATAAGCAGTGGTATCAACGCAGA38060.29669287999451205No Hit
GTACTCTGCGTTGATACCACTGCTT37240.29030065294260715No Hit
CCACTGCTTATGTACTCTGCGTTGA33820.26364038889685754No Hit
GGATACCACGTGTCCCGCCCTACTC31980.24929685502429044No Hit
GATACCACTGCTTATGTACTCTGCG31690.24703618935959237No Hit
GGTATCAACGCAGAGTACTTTTTTT31150.24282667398394772No Hit
GTTGATACCACTGCTTATGTACTCT30950.24126759421519042No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30400.2369801248511079No Hit
TATCAACGCAGAGTACTTTTTTTTT28560.22263659097854083No Hit
GTACATGGGATAAGCAGTGGTATCA28210.21990820138321557No Hit
ATACCACTGCTTATGTACTCTGCGT26400.2057985294759621No Hit
ATACAGGGTGACAGCCCCGTACACA26290.20494103560314558No Hit
GAGTACATGGGAAGCAGTGGTATCA25980.20252446196157178No Hit
GCTTATCCCATGTACTCTGCGTTGA24260.18911637595025912No Hit
TACCACTGCTTATGTACTCTGCGTT21870.1704853727136095No Hit
CAGTGGTATCAACGCAGAGTACATA21360.16650971930327843No Hit
GAGTACATGATAAGCAGTGGTATCA21170.165028593522959No Hit
TGATACCACTGCTTATGTACTCTGC20620.16074112415887645No Hit
ACCCTGTATCGCGCGCCTTTCCAGA20510.15988363028605995No Hit
GTATTTAGCCTTGGAGGATGGTCCC20150.15707728670229684No Hit
GCGTTGATACCACTGCTTCCCATGT19800.15434889710697158No Hit
GCAGAGTACATAAGCAGTGGTATCA19240.14998347375445117No Hit
ATCATTAACTGAATCCATAGGTTAA18990.14803462404350456No Hit
GCTTATCATGTACTCTGCGTTGATA18630.14522828045974143No Hit
GTATCAACGCAGAGTACATGGGAAG18420.1435912467025463No Hit
GATAAGCAGTGGTATCAACGCAGAG17770.1385242374540851No Hit
CTATCGGTCAGTCAGGAGTATTTAG17640.13751083560439287No Hit
CCCATATTCAGACAGGATACCACGT17580.13704311167376568No Hit
GAACAAAAAAAAAAAAAAAAAAAAA17520.1365753877431385No Hit
GGTTAATGAGGCGAACCGGGGGAAC17270.13462653803219188No Hit
CCCCATGTACTCTGCGTTGATACCA17000.13252178034436954No Hit
GTTCACTATCGGTCAGTCAGGAGTA16960.13220996439061808No Hit
TATCAACGCAGAGTACATGGGAAGC16920.13189814843686662No Hit
GGATAAGCAGTGGTATCAACGCAGA16840.1312745165293637No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA16730.1304170226565472No Hit
GTACATGGTAAGCAGTGGTATCAAC16480.1284681729456006No Hit
GCGTTGATACCACTGCTTATCATGT16300.12706500115371905No Hit
TATTCAGACAGGATACCACGTGTCC16190.12620750728090252No Hit
GGTATCAACGCAGAGTACATGGGAA15870.12371297965089086No Hit
GTCAGGAGTATTTAGCCTTGGAGGA15790.12308934774338794No Hit
TCTAAGTACCCCGAGGAAAAGAAAT15740.12269957780119863No Hit
ACGCAGAGTACATGGGAAGCAGTGG14830.11560576485335297No Hit
GTACACAAGCAGTGGTATCAACGCA14820.11552781086491509No Hit
ACTCTGCGTTGATACCACTGCTTCC14800.11537190288803936No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14680.11443645502678498No Hit
GCCTTGGAGGATGGTCCCCCCATAT14120.11007103167426457No Hit
ATGTACTCTGCGTTGATACCACTGC14050.10952535375519953No Hit
CAGTGGTATCAACGCAGAGTACATG13510.10531583837955485No Hit
GTTAATGATAGTGTGTCGAAACACA13440.10477016046048981No Hit
ACCATGTACTCTGCGTTGATACCAC13330.10391266658767329No Hit
ATCAACGCAGAGTACATAAGCAGTG13290.10360085063392183No Hit
GTACATAAGCAGTTGTATCAACGCA13230.10313312670329465No Hit
GGGATAAGCAGTGGTATCAACGCAG13140.10243154080735388No Hit
ACGCAGAGTACATGATAAGCAGTGG12930.10079450705015872No Hit
GAGTACATGGGATAAGCAGTGGTAT12880.1004047371079694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGG250.00603659418.99723210
GCGCATG402.7637157E-416.62257815
TAGCATA350.002168078216.2877865
ACACCGT601.468552E-615.8310277
GAGGTGT551.1295606E-515.5431896
CGACATG508.6535045E-515.21201919
TATTGAC508.702597E-515.2019352
AGTAGTA508.7228815E-515.19778611
TTGGGTT508.7228815E-515.19778614
CCGTCGC704.4415356E-714.92639510
CCATACC456.754667E-414.7796584
CACTCTA456.7686604E-414.77562513
TTCTATA653.3665656E-614.6172452
GCTTATA602.5485013E-514.2612691
AGAGGTG400.005273984714.2518135
GTCGCCA602.5710993E-514.24792512
GGACACT400.00528354414.2479246
TTCTATT759.65505E-713.9351052
TCTATAG759.65505E-713.9351053
GTACCAT551.946635E-413.8291081
Produced by FastQC (version 0.11.5)