##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295261_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1282808
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.536474671190078	31.0	30.0	33.0	24.0	34.0
2	29.449815560863357	31.0	30.0	34.0	22.0	34.0
3	29.82710273088412	31.0	30.0	34.0	25.0	34.0
4	32.76851095409445	35.0	33.0	37.0	22.0	37.0
5	33.54032793683856	35.0	33.0	37.0	27.0	37.0
6	33.531474702371675	36.0	35.0	37.0	27.0	37.0
7	33.581026934662084	36.0	35.0	37.0	27.0	37.0
8	33.463363964053855	36.0	33.0	37.0	27.0	37.0
9	34.28790668595768	38.0	34.0	39.0	23.0	39.0
10	34.2150734950203	38.0	33.0	39.0	23.0	39.0
11	34.70655936040311	38.0	34.0	39.0	27.0	39.0
12	34.72122562378782	38.0	34.0	39.0	26.0	39.0
13	34.44120476330051	38.0	34.0	39.0	25.0	39.0
14	34.56207632007284	38.0	32.0	40.0	18.0	41.0
15	35.12033991057118	38.0	33.0	40.0	24.0	41.0
16	35.06727273294211	38.0	33.0	40.0	24.0	41.0
17	35.119789555412815	38.0	33.0	40.0	25.0	41.0
18	35.05875703924516	38.0	33.0	40.0	24.0	41.0
19	35.14131888793958	39.0	34.0	40.0	24.0	41.0
20	35.20966660638225	39.0	34.0	41.0	24.0	41.0
21	35.20177376505291	39.0	34.0	41.0	24.0	41.0
22	34.93152677563595	39.0	33.0	40.0	23.0	41.0
23	34.598852673198174	38.0	32.0	40.0	21.0	41.0
24	34.92070364388123	38.0	33.0	40.0	23.0	41.0
25	33.66475419548366	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.49733007222616976
1101	2	0.6942768270480819
1101	3	0.4858160937849654
1101	4	0.43592571029180505
1101	5	0.3363458690698451
1101	6	0.2572346470104776
1101	7	0.21641010728608734
1101	8	0.317258818309476
1101	9	0.3145157225132138
1101	10	0.6851836513852732
1101	11	0.4555079713818202
1101	12	0.5889226668610519
1101	13	0.5444022842359075
1101	14	1.2229350746618053
1101	15	0.8524479955306603
1101	16	0.6062072699562577
1101	17	0.8645609878725367
1101	18	0.8461337290941557
1101	19	0.8128460658460881
1101	20	0.9734009558361763
1101	21	0.9935172840936417
1101	22	0.75848268937483
1101	23	-1.47844814522729
1101	24	0.21875136050561395
1101	25	0.637474373281826
1102	1	0.41226026792207904
1102	2	0.33630911111535866
1102	3	0.34957543320119555
1102	4	0.46619253140176653
1102	5	0.40454342100817087
1102	6	0.23664220795800617
1102	7	0.23114250718583662
1102	8	0.27928947665787973
1102	9	0.4354590458890186
1102	10	0.9397746137431113
1102	11	0.6916991830165458
1102	12	0.5969889420004222
1102	13	0.5081223354460533
1102	14	1.0779365310349576
1102	15	0.7777123441931693
1102	16	0.6283075632630357
1102	17	0.9064625370471475
1102	18	0.8377009898255992
1102	19	0.9055520071711882
1102	20	0.8658427178444796
1102	21	0.707816836343568
1102	22	0.6484282955308842
1102	23	0.8221784339215077
1102	24	0.7625766770127527
1102	25	0.5808621004591075
1103	1	-0.33721785319957576
1103	2	-1.2333409869692744
1103	3	-0.2654423821709244
1103	4	-0.05402205375457214
1103	5	-0.2832143013694193
1103	6	-0.2411586373427994
1103	7	-0.12387811060437315
1103	8	0.17413159757364127
1103	9	0.6922920846824923
1103	10	0.8189303424004066
1103	11	0.47594015665666234
1103	12	0.2692733130863303
1103	13	0.043981848511613464
1103	14	-1.0527419401029903
1103	15	-0.23568778963659298
1103	16	-0.08018519334409291
1103	17	0.2640796809827677
1103	18	0.3667103064359267
1103	19	0.326509519081462
1103	20	0.4798596696142923
1103	21	0.45648425794735203
1103	22	-0.013530022789495888
1103	23	0.9658023608527486
1103	24	0.6512077243736201
1103	25	0.5292475644880668
1104	1	0.13869532045312027
1104	2	0.16212907291096812
1104	3	-0.23080565562401745
1104	4	-0.10508492800607172
1104	5	-0.04966240516183262
1104	6	-0.03004785889968531
1104	7	-0.26695395890776297
1104	8	0.0554160287113632
1104	9	-2.101689951245646
1104	10	-0.43648183324829404
1104	11	-0.46517760781439677
1104	12	0.12979027915419294
1104	13	0.16798384451959691
1104	14	-1.7397339560710563
1104	15	-0.36791134253080315
1104	16	-0.06669816739599099
1104	17	0.25085413208057616
1104	18	0.3760935399688634
1104	19	0.42395363085790905
1104	20	0.5702319251033146
1104	21	0.6097666930770913
1104	22	0.8213112945757715
1104	23	1.1354301053637244
1104	24	0.6748364668885927
1104	25	0.498645768080884
1105	1	-0.12784883408777503
1105	2	-0.14122591956268948
1105	3	-1.3912567343997324
1105	4	-0.584928129210283
1105	5	-0.23753671524713127
1105	6	-0.25954334309436433
1105	7	-0.18505079784404188
1105	8	-0.28293595322541165
1105	9	0.1529538018903267
1105	10	0.39970456916013575
1105	11	-0.13865979547119878
1105	12	-0.012666820695280023
1105	13	-0.13037290410057523
1105	14	0.6610282115154931
1105	15	0.24437766900958735
1105	16	-0.3923133204316116
1105	17	0.1964727974092213
1105	18	-0.009678160984471162
1105	19	-0.11644451264434963
1105	20	-0.6964838300396821
1105	21	-0.1074628603599379
1105	22	0.08186749126418391
1105	23	0.15908013045153524
1105	24	0.06927500176064427
1105	25	0.28498771489473285
1106	1	-1.7140764488203146
1106	2	-0.8887813541550358
1106	3	-1.2325854670494216
1106	4	-1.4470164310565394
1106	5	-1.0267164817009373
1106	6	-0.7806022127543741
1106	7	-0.555449244542821
1106	8	-0.605632501516034
1106	9	-0.3078158525213013
1106	10	-1.9609773961989276
1106	11	-1.408466570654106
1106	12	-0.8922879238408186
1106	13	-1.0415974089468492
1106	14	-0.23671177082424322
1106	15	-1.0279601500737883
1106	16	-1.6449682727759125
1106	17	-0.6982983914912495
1106	18	-0.5485614794432294
1106	19	-0.538779868864772
1106	20	-0.29057201277799294
1106	21	-0.04773940786578379
1106	22	-0.273068350460278
1106	23	-0.3834276317410996
1106	24	-0.8488263173598796
1106	25	-1.673736650111021
1107	1	-0.1910066260189005
1107	2	0.028466055879334817
1107	3	-0.13940176267998083
1107	4	-0.04995719077979288
1107	5	-0.3260723808309365
1107	6	0.034474038910531135
1107	7	-0.19811940902941672
1107	8	-0.29338810754167355
1107	9	0.06433194656457175
1107	10	-0.05807185757919342
1107	11	0.07654988610288171
1107	12	-0.3177985286316982
1107	13	-0.07940058370424907
1107	14	-1.2881208173849217
1107	15	-0.7647872549395629
1107	16	-0.40362517469525727
1107	17	-0.37667384845470764
1107	18	0.01901081490803591
1107	19	-0.13776048106422678
1107	20	0.00881708325416497
1107	21	0.1899625568240495
1107	22	-0.7317556153998979
1107	23	0.2797889861423144
1107	24	0.1306320873265463
1107	25	-0.36085179882909557
1108	1	-0.04049028560748624
1108	2	0.198769224426119
1108	3	-0.15530186774523003
1108	4	-0.3952175037636465
1108	5	-0.22914346576789768
1108	6	-0.19616907102089698
1108	7	0.02105361684981233
1108	8	-0.09403094098560416
1108	9	0.37128353360283484
1108	10	0.30105983341837117
1108	11	0.003098907337118817
1108	12	0.14478649127540422
1108	13	-0.02425100396525437
1108	14	0.19042513483803702
1108	15	0.14976289989343883
1108	16	0.007267741694917618
1108	17	0.438407162383605
1108	18	0.3232942975446207
1108	19	0.42452938843366894
1108	20	0.40973571298210487
1108	21	0.42132729913770106
1108	22	0.31439084003032036
1108	23	-0.037463834030212695
1108	24	0.08920389113101379
1108	25	-0.6731686258585086
1109	1	-0.9184843310076687
1109	2	-0.6431530900823716
1109	3	-0.90146326710531
1109	4	-0.7792481776216498
1109	5	-1.1603925435524047
1109	6	-1.433785173097533
1109	7	-1.0795469881235533
1109	8	-1.543942505373721
1109	9	-1.2357058405331927
1109	10	-1.4658839347347126
1109	11	-1.6101032869532972
1109	12	-1.3512035701538565
1109	13	-1.3432283543527745
1109	14	-0.8565801887722344
1109	15	-1.5647109324846724
1109	16	-1.4094234364887583
1109	17	-1.362510296417632
1109	18	-2.3988429028809577
1109	19	-2.254721151305084
1109	20	-1.9458887822262554
1109	21	-1.8351689809362313
1109	22	-1.6599703558598975
1109	23	-1.2474804533241297
1109	24	-2.0254136612508233
1109	25	-1.1511458772292116
1110	1	0.038190454273070884
1110	2	0.21289427972361707
1110	3	-0.02845285757049254
1110	4	0.43033478002313075
1110	5	0.21018066053159856
1110	6	0.21598255472562045
1110	7	0.23351076128687964
1110	8	0.16792576107145862
1110	9	0.5528964234502141
1110	10	0.29521281395365406
1110	11	0.3709537790469142
1110	12	0.37952507234154353
1110	13	0.4519862776096204
1110	14	-2.5212108417158277
1110	15	-0.4986072062777609
1110	16	-0.08537699951277489
1110	17	-0.049179931179764935
1110	18	-2.5357337106394127
1110	19	-0.2925050066116768
1110	20	-1.7848568826825755
1110	21	-0.40809097115648285
1110	22	-1.7585196056675372
1110	23	-0.35670736422265037
1110	24	0.1573960775941856
1110	25	-0.010973453330308303
1111	1	-0.1518864711729755
1111	2	-1.135362163700524
1111	3	-0.18920974520532496
1111	4	-0.014551821871698678
1111	5	-0.044556757644798495
1111	6	0.16669459484909765
1111	7	0.09905090964140584
1111	8	0.134155561330644
1111	9	-1.511918773056749
1111	10	-0.15779585466990653
1111	11	0.03457896472698252
1111	12	0.030507903302193995
1111	13	0.002088031471885188
1111	14	0.9549505619425744
1111	15	0.5231188424740623
1111	16	0.5149094275407222
1111	17	0.07646464814678922
1111	18	0.28001077463293456
1111	19	0.07305148869627232
1111	20	0.42080105850156
1111	21	-0.2592221092598095
1111	22	-0.17031334028303746
1111	23	-0.012870284120211295
1111	24	-0.4146240978631113
1111	25	-1.8587539398061672
1112	1	0.2541604367321888
1112	2	0.40226860779468865
1112	3	0.1191982203449733
1112	4	0.45125228129625583
1112	5	0.3013027111172377
1112	6	0.06101028063519465
1112	7	0.21879410310774006
1112	8	0.0890865713470248
1112	9	-0.06039150163324791
1112	10	0.6374212675269035
1112	11	0.30662471776700073
1112	12	0.18908485279760612
1112	13	0.09773190653510255
1112	14	0.5818319354018087
1112	15	0.32860803731415444
1112	16	0.3042088093481965
1112	17	-3.290781526834156
1112	18	-0.7449645995660177
1112	19	-0.8747130358087531
1112	20	0.12028231270021195
1112	21	-0.28211702785313975
1112	22	-1.517522038757562
1112	23	-1.300104778357202
1112	24	-0.1849658586928058
1112	25	0.1741380161428978
1113	1	0.023815291369260194
1113	2	0.11379097305638552
1113	3	0.10463351063870618
1113	4	-0.04645792849080266
1113	5	0.18296071650564016
1113	6	0.26198340624815586
1113	7	-0.03481484111814126
1113	8	-0.009043738617769748
1113	9	0.520925172360748
1113	10	0.6775872409155426
1113	11	0.25476907177842634
1113	12	0.25691243135971575
1113	13	-0.1618149921260681
1113	14	-1.372536185833539
1113	15	-1.4220208917309947
1113	16	-0.1851862914871205
1113	17	0.08286479616200637
1113	18	-0.2460164939623084
1113	19	0.43903918587487567
1113	20	0.6933926134213024
1113	21	-1.2327216733795225
1113	22	-1.434915801885687
1113	23	-0.10558322473806925
1113	24	-2.007201342127402
1113	25	-0.020314726344714984
1114	1	0.1089937355100794
1114	2	0.14232743843845697
1114	3	0.019587113073356477
1114	4	0.44237297194440117
1114	5	0.07927246358507034
1114	6	0.18104005590962657
1114	7	0.059415035643993974
1114	8	0.007398941044201024
1114	9	0.5867275821867395
1114	10	0.4248448136065832
1114	11	0.23133056454815915
1114	12	0.21423257732160295
1114	13	0.18092341856615235
1114	14	0.9935384560141358
1114	15	0.5843600789649841
1114	16	0.24081396732417204
1114	17	-0.5783216975380512
1114	18	-0.0749064600311371
1114	19	-0.4447043681514984
1114	20	-1.7139451427520598
1114	21	-0.811776397561836
1114	22	-1.5617270907635046
1114	23	-2.859585496419335
1114	24	-2.076610784225821
1114	25	-1.39447037852635
1115	1	0.5124548237475963
1115	2	0.5508890830478137
1115	3	0.543791911532594
1115	4	0.687121103418157
1115	5	0.35974281531459695
1115	6	0.48690373765378325
1115	7	0.5752219153953249
1115	8	0.4807249491371124
1115	9	0.8338422332449724
1115	10	0.9843440663969147
1115	11	0.6629268017142422
1115	12	0.6429494276590049
1115	13	0.48824486834119085
1115	14	1.1837627494535283
1115	15	0.8248975778949443
1115	16	0.6703464347327497
1115	17	0.6350903206259346
1115	18	0.8997514213677462
1115	19	0.10838165924108267
1115	20	0.8403614233293695
1115	21	-0.13404906972067465
1115	22	0.8884164643679711
1115	23	1.0410583364838502
1115	24	1.1334659651146453
1115	25	1.4021173849080526
1116	1	0.42139491103653626
1116	2	0.507575848755387
1116	3	0.4282209564537709
1116	4	0.7601683985036516
1116	5	0.5718509008770596
1116	6	0.3490509128505721
1116	7	0.4186519776326634
1116	8	0.2796130604411502
1116	9	0.5210787283858735
1116	10	0.587198310047512
1116	11	0.5785787893196854
1116	12	0.4557749260323902
1116	13	-0.025593432860134158
1116	14	1.0021196775011063
1116	15	0.664215576609422
1116	16	0.5603857630031897
1116	17	0.7652881136568297
1116	18	0.889269589098852
1116	19	1.0647913995375404
1116	20	0.958522099105366
1116	21	0.3469964781333843
1116	22	0.4977457879227529
1116	23	-0.5018569980139631
1116	24	0.0515242867452983
1116	25	-0.4349514683571911
1201	1	0.3092334740094067
1201	2	0.5033671793640977
1201	3	0.3268161060340127
1201	4	0.16947819022285415
1201	5	0.21097419399031025
1201	6	0.04271262155250355
1201	7	-0.0037589518422933565
1201	8	-0.027669463516616588
1201	9	0.19956063754379727
1201	10	0.6563182138548598
1201	11	0.1006490525718462
1201	12	0.21557124947920414
1201	13	0.17322407061474365
1201	14	0.8525924687994078
1201	15	0.42013010824639707
1201	16	0.23388544070575534
1201	17	0.12602982685212538
1201	18	0.1672037613536972
1201	19	0.3014625804575317
1201	20	0.5002792637750062
1201	21	0.38568591116470685
1201	22	0.3804603760642351
1201	23	0.19938653494450875
1201	24	0.4740244084806804
1201	25	0.37960408178790317
1202	1	0.39452549123110714
1202	2	0.7446271753777616
1202	3	0.39674462900596197
1202	4	0.29572626743985353
1202	5	0.29615068781728837
1202	6	0.26939142003769234
1202	7	0.28216748321747076
1202	8	0.192731967990305
1202	9	0.7691734263634586
1202	10	0.003670918174854876
1202	11	0.2174599633430958
1202	12	0.39077130382212744
1202	13	0.4386195181438879
1202	14	1.1828296112913748
1202	15	0.7826351470327637
1202	16	0.5989773544256423
1202	17	-1.4229561145987404
1202	18	0.11010302763489932
1202	19	0.4222904430021259
1202	20	0.6684947334524622
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2210	18	0.8144158217826103
2210	19	0.9612308127204159
2210	20	0.9009966740062936
2210	21	1.2930823927357977
2210	22	1.4938951561067313
2210	23	1.8534171848371557
2210	24	1.5953688900967862
2210	25	1.5354114921179374
2211	1	-0.0102410072862682
2211	2	-0.8049948165263494
2211	3	-0.1724038787237241
2211	4	-0.04109430609096165
2211	5	0.032644110139251836
2211	6	-0.06036819453640874
2211	7	0.09272323753843636
2211	8	0.1691322182473982
2211	9	0.6102597032459585
2211	10	0.6861731519442031
2211	11	0.35491123720041173
2211	12	0.150721177476008
2211	13	0.08098976752058462
2211	14	-1.315865249259602
2211	15	-0.2976527048210329
2211	16	3.3934494359755263E-4
2211	17	0.25641977867484655
2211	18	0.13733440867567737
2211	19	0.42010022513234446
2211	20	0.480800039614671
2211	21	0.3809132873515253
2211	22	-0.3058570173689432
2211	23	0.3111107568938323
2211	24	-0.3939137799031158
2211	25	0.06361072365837828
2212	1	-0.025411649537200987
2212	2	-0.9567542765566017
2212	3	-0.1997301473955595
2212	4	0.18191652407524828
2212	5	0.08749780781008809
2212	6	0.17658863674407144
2212	7	0.0892638532897152
2212	8	0.12194507033769497
2212	9	-2.1985873374992764
2212	10	0.006867150294787905
2212	11	0.13332190815849998
2212	12	0.014420482445579808
2212	13	0.042983844519596914
2212	14	0.7559475540935097
2212	15	0.2715388026773269
2212	16	0.2665728877830986
2212	17	0.3139306083632434
2212	18	0.04706837055937285
2212	19	-0.8314127776609652
2212	20	0.19644247689421945
2212	21	0.44643803867244714
2212	22	-1.563907969703024
2212	23	-0.23650164681439634
2212	24	0.22430016485567705
2212	25	-0.17905074692395573
2213	1	0.026993948576588878
2213	2	0.19257958736466563
2213	3	0.05203461875128568
2213	4	0.1705801871335666
2213	5	0.245835145058642
2213	6	0.11636247916988651
2213	7	0.09116510037689807
2213	8	0.23206301548255226
2213	9	0.6191258253344571
2213	10	0.6344008223127062
2213	11	0.013386821386973224
2213	12	-0.034653130938899324
2213	13	-0.029284651315776955
2213	14	-2.058448801734947
2213	15	-0.5148961538977801
2213	16	-0.22901418895404646
2213	17	-2.5062913848229016
2213	18	-1.1513395810502445
2213	19	-0.7555414695830507
2213	20	0.09358493833212833
2213	21	-0.19159268957847075
2213	22	0.17850115248856468
2213	23	0.7483289294695581
2213	24	0.7198594948160775
2213	25	-0.9981246875781054
2214	1	0.0543871838383545
2214	2	0.07507457768243953
2214	3	0.1626785231806025
2214	4	0.3218399840984034
2214	5	0.26906461643535096
2214	6	0.20784691759453722
2214	7	0.09511083265426379
2214	8	0.11001748526585686
2214	9	0.5190709729130631
2214	10	0.48772534907215004
2214	11	-0.002972685664722974
2214	12	-0.014950554597689347
2214	13	-0.051692197670256235
2214	14	1.0319149640695784
2214	15	0.4582081953898367
2214	16	0.4180145397863342
2214	17	0.25756734152306393
2214	18	0.7226826911491742
2214	19	-0.4840697242400296
2214	20	0.38288310541472015
2214	21	0.6691089833834738
2214	22	0.7071975326470934
2214	23	1.2462294524255029
2214	24	1.1476099475515724
2214	25	1.0001608358860068
2215	1	0.02645173070952822
2215	2	0.4306034318853271
2215	3	0.26619434437598244
2215	4	0.6925432771221338
2215	5	0.4900683640689323
2215	6	0.34114444879261896
2215	7	0.474558861605054
2215	8	0.2631980799934155
2215	9	0.5012587667030672
2215	10	0.9221976539311925
2215	11	0.6284121357753492
2215	12	0.3700210483849631
2215	13	0.3660607675965224
2215	14	1.1965993772622738
2215	15	0.7407040420845803
2215	16	0.5709172172193959
2215	17	0.8148028500292952
2215	18	0.7004781553534798
2215	19	0.0965049129091966
2215	20	-1.0960116646402724
2215	21	-1.5847333069229066
2215	22	0.2252984740629529
2215	23	0.9142377976714187
2215	24	1.0391441591962831
2215	25	0.12399192192703623
2216	1	0.2230820649934948
2216	2	-0.21748215106059376
2216	3	0.09610800613321757
2216	4	0.45494173527478665
2216	5	0.5200656293734625
2216	6	0.25588992677832323
2216	7	0.35614863123952034
2216	8	0.156090736684952
2216	9	0.5365350360575505
2216	10	-1.3097964599623637
2216	11	-0.2586006652039359
2216	12	0.007870015060746027
2216	13	0.1427806957130997
2216	14	-0.26673903479122174
2216	15	-0.3961307432418266
2216	16	0.25794225921650593
2216	17	0.6452644926697033
2216	18	0.39030430685560447
2216	19	-1.1084717119557013
2216	20	-0.6572584253283722
2216	21	-0.5829049676644047
2216	22	-0.5487138450890328
2216	23	-4.312137949486452
2216	24	-1.7135698307244098
2216	25	-0.7958064919495911
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	6.0
4	14.0
5	27.0
6	32.0
7	114.0
8	96.0
9	253.0
10	622.0
11	1327.0
12	2818.0
13	4902.0
14	7386.0
15	10188.0
16	13099.0
17	14745.0
18	14840.0
19	14231.0
20	13705.0
21	13149.0
22	12696.0
23	12498.0
24	12887.0
25	13289.0
26	14449.0
27	16008.0
28	18186.0
29	22327.0
30	27180.0
31	33409.0
32	43856.0
33	57854.0
34	78422.0
35	106648.0
36	149600.0
37	218777.0
38	333165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.69461128764114	18.166435092903857	11.613820779850977	20.52513283960403
2	12.076472055888225	23.759590194610777	39.81934568363273	24.344592065868266
3	19.94555503916107	32.372527674758814	30.598582653096233	17.08333463298388
4	12.464116903443763	21.100547536475787	31.860524421561752	34.5748111385187
5	9.202800889745774	46.80329505429883	32.65221921688645	11.34168483906895
6	27.980542298824947	32.952797375908034	24.678596294803352	14.388064030463669
7	22.473499335290427	39.59953684752225	19.675403406587836	18.251560410599485
8	21.59154002595199	38.75798522732944	24.481662050207117	15.168812696511456
9	27.66690139032829	22.956208050943612	17.12843283355129	32.248457725176806
10	14.588612875507032	29.534818077048435	28.497909496236158	27.378659551208372
11	27.216459154648437	30.50988073257149	20.04045236788263	22.233207744897452
12	25.730703707024595	28.597848841969025	24.097570103659464	21.57387734734692
13	28.259760958660568	22.268990125197277	31.44107868028221	18.030170235859945
14	28.53429721130451	26.883071308253793	22.745024642834863	21.837606837606838
15	31.821283385178532	24.478786097011486	23.56670563180054	20.133224886009437
16	23.48935201357353	25.87072396327162	30.10972353909876	20.530200484056092
17	19.828145468155448	34.38595533653546	26.343958580250764	19.441940615058325
18	20.280864269729506	24.862491910142147	36.936224629036154	17.92041919109219
19	22.113101039319492	34.25466056433567	22.50302127759109	21.12921711875375
20	20.474159270922428	31.700279196979274	28.85638391182958	18.969177620268717
21	21.684782862925548	33.47634499308461	24.199272441499836	20.63959970249001
22	19.841118857565217	31.162550269220034	20.9264973951227	28.069833478092054
23	24.43304885065985	25.940266435462767	23.317469252948396	26.309215460928986
24	18.523008839897894	31.277239718882203	20.889541039487163	29.31021040173274
25	23.06093701835095	32.84199765101587	22.130630050608513	21.96643528002466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7283.0
1	7283.0
2	6905.0
3	6527.0
4	6527.0
5	6527.0
6	9168.5
7	11810.0
8	11810.0
9	11810.0
10	10989.0
11	10168.0
12	10168.0
13	10168.0
14	10623.0
15	11078.0
16	11078.0
17	11078.0
18	13952.0
19	16826.0
20	16826.0
21	16826.0
22	21385.0
23	25944.0
24	25944.0
25	25944.0
26	33095.5
27	40247.0
28	40247.0
29	40247.0
30	44635.5
31	49024.0
32	49024.0
33	49024.0
34	55540.5
35	62057.0
36	62057.0
37	62057.0
38	87965.0
39	113873.0
40	113873.0
41	113873.0
42	238320.0
43	362767.0
44	362767.0
45	362767.0
46	291387.0
47	220007.0
48	220007.0
49	220007.0
50	178755.5
51	137504.0
52	137504.0
53	137504.0
54	107129.5
55	76755.0
56	76755.0
57	76755.0
58	64719.0
59	52683.0
60	52683.0
61	52683.0
62	46581.0
63	40479.0
64	40479.0
65	40479.0
66	29936.0
67	19393.0
68	19393.0
69	19393.0
70	15615.0
71	11837.0
72	11837.0
73	11837.0
74	8239.0
75	4641.0
76	4641.0
77	4641.0
78	2990.5
79	1340.0
80	1340.0
81	1340.0
82	864.0
83	388.0
84	388.0
85	388.0
86	257.5
87	127.0
88	127.0
89	127.0
90	81.0
91	35.0
92	35.0
93	35.0
94	23.0
95	11.0
96	11.0
97	11.0
98	7.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07787603444942656
2	0.01933258913259038
3	0.03211704323640015
4	0.04045811999925164
5	0.0502023685539847
6	0.018007371329146683
7	0.02284051861229428
8	0.03429975491266035
9	0.03500134080860113
10	0.026660264045749633
11	0.024321644392613705
12	0.01894281919040106
13	0.02743980393012828
14	0.03804154635767785
15	0.03624860462360696
16	0.024321644392613705
17	0.025568908207619537
18	0.026348448091998183
19	0.018553049248211738
20	0.015668751676010752
21	0.012628546126934039
22	0.01668215352570299
23	0.01917668115571465
24	0.01668215352570299
25	0.1093694457783238
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1282808.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.055790856418394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53341007127045	16.53583057702013
2	7.274369398328042	3.063352013270507
3	3.217592213904615	2.0324684615164745
4	1.939980517730739	1.6339129618745885
5	1.3516647199052167	1.4230184825161796
6	0.9842698092046823	1.2434747549340377
7	0.7557061240267966	1.1138393067495966
8	0.6190253502981926	1.04272546485599
9	0.502144861002027	0.9515751455595569
>10	4.109011390960441	17.143207120693685
>50	0.40114923118827644	5.878773949749181
>100	0.24216842761349888	9.973036793588157
>500	0.03364477391271511	4.8394244922168435
>1k	0.02883837763947009	12.115415334982952
>5k	0.0040669506927457815	6.149140210982326
>10k+	0.002957782321996932	14.860804929489815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	63819	4.9749455881160705	No Hit
GCTTATGTACTCTGCGTTGATACCA	37255	2.9041758392526393	No Hit
CTTATGTACTCTGCGTTGATACCAC	24954	1.9452638274784693	No Hit
GAGTACATAAGCAGTGGTATCAACG	15761	1.2286328117691814	No Hit
TATGTACTCTGCGTTGATACCACTG	14725	1.1478724797475537	No Hit
CCCATGTACTCTGCGTTGATACCAC	12883	1.0042812330450075	No Hit
GCGTTGATACCACTGCTTATGTACT	11285	0.8797107595213003	No Hit
CATAAGCAGTGGTATCAACGCAGAG	10213	0.7961440839159095	No Hit
CTGCTTATGTACTCTGCGTTGATAC	8777	0.6842021565191362	No Hit
ACTCTGCGTTGATACCACTGCTTAT	8583	0.6690790827621904	No Hit
CATGTACTCTGCGTTGATACCACTG	8167	0.6366502235720388	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	8030	0.6259705271560514	No Hit
GTATCAACGCAGAGTACATAAGCAG	7271	0.5668034499317123	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6586	0.5134049678517751	No Hit
GTACATGATAAGCAGTGGTATCAAC	6462	0.5037386732854799	No Hit
TATCAACGCAGAGTACATAAGCAGT	6386	0.49781417016420226	No Hit
ATCATGTACTCTGCGTTGATACCAC	6331	0.49352670080011973	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6271	0.4888494614938479	No Hit
GGTATCAACGCAGAGTACATAAGCA	6125	0.47746817918191964	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4987	0.3887565403396299	No Hit
GTGGTATCAACGCAGAGTACATAAG	4681	0.36490261987764344	No Hit
GTACTGGTTCACTATCGGTCAGTCA	4377	0.3412046073925326	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4125	0.3215602023061908	No Hit
GTGGTATCAACGCAGAGTACATGGG	3901	0.3040985088961091	No Hit
GCAGTGGTATCAACGCAGAGTACAT	3855	0.3005126254279674	No Hit
ACATAAGCAGTGGTATCAACGCAGA	3806	0.29669287999451205	No Hit
GTACTCTGCGTTGATACCACTGCTT	3724	0.29030065294260715	No Hit
CCACTGCTTATGTACTCTGCGTTGA	3382	0.26364038889685754	No Hit
GGATACCACGTGTCCCGCCCTACTC	3198	0.24929685502429044	No Hit
GATACCACTGCTTATGTACTCTGCG	3169	0.24703618935959237	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3115	0.24282667398394772	No Hit
GTTGATACCACTGCTTATGTACTCT	3095	0.24126759421519042	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3040	0.2369801248511079	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2856	0.22263659097854083	No Hit
GTACATGGGATAAGCAGTGGTATCA	2821	0.21990820138321557	No Hit
ATACCACTGCTTATGTACTCTGCGT	2640	0.2057985294759621	No Hit
ATACAGGGTGACAGCCCCGTACACA	2629	0.20494103560314558	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2598	0.20252446196157178	No Hit
GCTTATCCCATGTACTCTGCGTTGA	2426	0.18911637595025912	No Hit
TACCACTGCTTATGTACTCTGCGTT	2187	0.1704853727136095	No Hit
CAGTGGTATCAACGCAGAGTACATA	2136	0.16650971930327843	No Hit
GAGTACATGATAAGCAGTGGTATCA	2117	0.165028593522959	No Hit
TGATACCACTGCTTATGTACTCTGC	2062	0.16074112415887645	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2051	0.15988363028605995	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	2015	0.15707728670229684	No Hit
GCGTTGATACCACTGCTTCCCATGT	1980	0.15434889710697158	No Hit
GCAGAGTACATAAGCAGTGGTATCA	1924	0.14998347375445117	No Hit
ATCATTAACTGAATCCATAGGTTAA	1899	0.14803462404350456	No Hit
GCTTATCATGTACTCTGCGTTGATA	1863	0.14522828045974143	No Hit
GTATCAACGCAGAGTACATGGGAAG	1842	0.1435912467025463	No Hit
GATAAGCAGTGGTATCAACGCAGAG	1777	0.1385242374540851	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1764	0.13751083560439287	No Hit
CCCATATTCAGACAGGATACCACGT	1758	0.13704311167376568	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	1752	0.1365753877431385	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1727	0.13462653803219188	No Hit
CCCCATGTACTCTGCGTTGATACCA	1700	0.13252178034436954	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1696	0.13220996439061808	No Hit
TATCAACGCAGAGTACATGGGAAGC	1692	0.13189814843686662	No Hit
GGATAAGCAGTGGTATCAACGCAGA	1684	0.1312745165293637	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1673	0.1304170226565472	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1648	0.1284681729456006	No Hit
GCGTTGATACCACTGCTTATCATGT	1630	0.12706500115371905	No Hit
TATTCAGACAGGATACCACGTGTCC	1619	0.12620750728090252	No Hit
GGTATCAACGCAGAGTACATGGGAA	1587	0.12371297965089086	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	1579	0.12308934774338794	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1574	0.12269957780119863	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1483	0.11560576485335297	No Hit
GTACACAAGCAGTGGTATCAACGCA	1482	0.11552781086491509	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1480	0.11537190288803936	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1468	0.11443645502678498	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1412	0.11007103167426457	No Hit
ATGTACTCTGCGTTGATACCACTGC	1405	0.10952535375519953	No Hit
CAGTGGTATCAACGCAGAGTACATG	1351	0.10531583837955485	No Hit
GTTAATGATAGTGTGTCGAAACACA	1344	0.10477016046048981	No Hit
ACCATGTACTCTGCGTTGATACCAC	1333	0.10391266658767329	No Hit
ATCAACGCAGAGTACATAAGCAGTG	1329	0.10360085063392183	No Hit
GTACATAAGCAGTTGTATCAACGCA	1323	0.10313312670329465	No Hit
GGGATAAGCAGTGGTATCAACGCAG	1314	0.10243154080735388	No Hit
ACGCAGAGTACATGATAAGCAGTGG	1293	0.10079450705015872	No Hit
GAGTACATGGGATAAGCAGTGGTAT	1288	0.1004047371079694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	7.795398843786444E-5	0.0
12	0.0	0.0	0.0	1.5590797687572888E-4	0.0
13	0.0	0.0	0.0	2.338619653135933E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTCGG	25	0.006036594	18.997232	10
GCGCATG	40	2.7637157E-4	16.622578	15
TAGCATA	35	0.0021680782	16.287786	5
ACACCGT	60	1.468552E-6	15.831027	7
GAGGTGT	55	1.1295606E-5	15.543189	6
CGACATG	50	8.6535045E-5	15.212019	19
TATTGAC	50	8.702597E-5	15.201935	2
AGTAGTA	50	8.7228815E-5	15.197786	11
TTGGGTT	50	8.7228815E-5	15.197786	14
CCGTCGC	70	4.4415356E-7	14.926395	10
CCATACC	45	6.754667E-4	14.779658	4
CACTCTA	45	6.7686604E-4	14.775625	13
TTCTATA	65	3.3665656E-6	14.617245	2
GCTTATA	60	2.5485013E-5	14.261269	1
AGAGGTG	40	0.0052739847	14.251813	5
GTCGCCA	60	2.5710993E-5	14.247925	12
GGACACT	40	0.005283544	14.247924	6
TTCTATT	75	9.65505E-7	13.935105	2
TCTATAG	75	9.65505E-7	13.935105	3
GTACCAT	55	1.946635E-4	13.829108	1
>>END_MODULE