FastQCFastQC Report
Wed 25 May 2016
SRR1295261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282808
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA687655.360506014929748No Hit
GCTTATGTACTCTGCGTTGATACCA422143.290749667916009No Hit
CTTATGTACTCTGCGTTGATACCAC292922.2834282293219252No Hit
GAGTACATAAGCAGTGGTATCAACG175871.3709767946567217No Hit
CCCATGTACTCTGCGTTGATACCAC158791.2378313824048492No Hit
TATGTACTCTGCGTTGATACCACTG134971.0521449819458562No Hit
GCGTTGATACCACTGCTTATGTACT130281.0155845613684977No Hit
CATAAGCAGTGGTATCAACGCAGAG129841.0121545858772318No Hit
GTATCAACGCAGAGTACTTTTTTTT116310.9066828395208013No Hit
CTGCTTATGTACTCTGCGTTGATAC102180.7965338538580988No Hit
ACGCAGAGTACATAAGCAGTGGTAT99320.7742390131648695No Hit
ACTCTGCGTTGATACCACTGCTTAT97660.761298651084184No Hit
GGTATCAACGCAGAGTACTTTTTTT92060.71764441755898No Hit
CATGTACTCTGCGTTGATACCACTG91750.7152278439174062No Hit
TATCAACGCAGAGTACTTTTTTTTT85200.664167981490605No Hit
GTATCAACGCAGAGTACATAAGCAG78750.6138876589481824No Hit
GTACTTTTTTTTTTTTTTTTTTTTT73140.5701554714345405No Hit
TATCAACGCAGAGTACATAAGCAGT73100.569843655480789No Hit
ATCATGTACTCTGCGTTGATACCAC69730.5435731613772288No Hit
GGTATCAACGCAGAGTACATAAGCA67180.5236948943255733No Hit
GTACATGGGAAGCAGTGGTATCAAC65380.5096631764067576No Hit
GTACATGATAAGCAGTGGTATCAAC64470.502569363458912No Hit
AAGCAGTGGTATCAACGCAGAGTAC59400.4630466913209148No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49850.38860063236275416No Hit
GTGGTATCAACGCAGAGTACATAAG49820.3883667703974406No Hit
ACATAAGCAGTGGTATCAACGCAGA48780.3802595555999027No Hit
GTACTGGTTCACTATCGGTCAGTCA46600.36326558612044824No Hit
GCAGTGGTATCAACGCAGAGTACAT44140.3440889049647336No Hit
GTGGTATCAACGCAGAGTACATGGG40640.31680500901148106No Hit
GTACTCTGCGTTGATACCACTGCTT37660.29357472045699745No Hit
CCACTGCTTATGTACTCTGCGTTGA37530.2925613186073052No Hit
GGATACCACGTGTCCCGCCCTACTC36860.2873384013819683No Hit
GATACCACTGCTTATGTACTCTGCG36780.2867147694744654No Hit
GTACATGGGATAAGCAGTGGTATCA30050.23425173525578263No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29710.23160129964889523No Hit
GTTGATACCACTGCTTATGTACTCT29530.23019812785701368No Hit
ATACAGGGTGACAGCCCCGTACACA29500.2299642658917001No Hit
ATACCACTGCTTATGTACTCTGCGT29380.22902881803044572No Hit
GAGTACATGGGAAGCAGTGGTATCA28970.22583270450449328No Hit
CAGTGGTATCAACGCAGAGTACATA28770.22427362473573598No Hit
GCTTATCCCATGTACTCTGCGTTGA26720.2082930571059738No Hit
TACCACTGCTTATGTACTCTGCGTT25330.19745745271311063No Hit
GAGTACATGATAAGCAGTGGTATCA25280.1970676827709213No Hit
GCAGAGTACATAAGCAGTGGTATCA23900.186310032366496No Hit
ACCCTGTATCGCGCGCCTTTCCAGA23870.1860761704011824No Hit
TGATACCACTGCTTATGTACTCTGC23410.18249028693304065No Hit
CCCATATTCAGACAGGATACCACGT22340.17414921017018917No Hit
GCGTTGATACCACTGCTTCCCATGT22320.17399330219331344No Hit
ATCATTAACTGAATCCATAGGTTAA21720.16931606288704157No Hit
CCCCATGTACTCTGCGTTGATACCA21470.16736721317609493No Hit
GATAAGCAGTGGTATCAACGCAGAG20910.16300178982357455No Hit
GCTTATCATGTACTCTGCGTTGATA20870.1626899738698231No Hit
GTATTTAGCCTTGGAGGATGGTCCC20560.16027340022824926No Hit
GTATCAACGCAGAGTACATGGGAAG20450.15941590635543276No Hit
CTATCGGTCAGTCAGGAGTATTTAG20240.1577788725982376No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20070.15645365479479392No Hit
GGATAAGCAGTGGTATCAACGCAGA19990.155830022887291No Hit
ACGCAGAGTACATGGGAAGCAGTGG19290.15037324369664049No Hit
TATTCAGACAGGATACCACGTGTCC18330.1428896608066055No Hit
GGTTAATGAGGCGAACCGGGGGAAC18280.1424998908644162No Hit
GCGTTGATACCACTGCTTATCATGT18240.14218807491066474No Hit
TATCAACGCAGAGTACATGGGAAGC18080.1409408110956589No Hit
GTCAGGAGTATTTAGCCTTGGAGGA18070.14086285710722102No Hit
GGTATCAACGCAGAGTACATGGGAA17810.13883605340783656No Hit
CAGTGGTATCAACGCAGAGTACATG17290.1347824460090676No Hit
GGGATAAGCAGTGGTATCAACGCAG17230.13431472207844042No Hit
TCTAAGTACCCCGAGGAAAAGAAAT17080.13314541225187246No Hit
GCCTTGGAGGATGGTCCCCCCATAT17080.13314541225187246No Hit
GTACATGGTAAGCAGTGGTATCAAC16890.13166428647155304No Hit
ACCATGTACTCTGCGTTGATACCAC16840.1312745165293637No Hit
ACTCTGCGTTGATACCACTGCTTCC16700.1301831606912336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16490.12854612693403844No Hit
ATCAACGCAGAGTACATAAGCAGTG15870.12371297965089086No Hit
ACGCAGAGTACATGATAAGCAGTGG15870.12371297965089086No Hit
CCATGTACTCTGCGTTGATACCACT15750.12277753178963648No Hit
GTACACAAGCAGTGGTATCAACGCA15680.12223185387057144No Hit
GTTCACTATCGGTCAGTCAGGAGTA15430.12028300415962481No Hit
GAGTACATGGGATAAGCAGTGGTAT15380.1198932342174355No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG14850.1157616728302287No Hit
GTTAATGATAGTGTGTCGAAACACA14770.11513804092272578No Hit
ATGTACTCTGCGTTGATACCACTGC14530.11326714520021704No Hit
AAAAAGTACTCTGCGTTGATACCAC14400.11225374335052478No Hit
TATCAACGCAGAGTACATGATAAGC14000.1091355838130102No Hit
CGGTACTGGTTCACTATCGGTCAGT13970.10890172184769661No Hit
TACATAAGCAGTGGTATCAACGCAG13960.10882376785925875No Hit
GGTATCAACGCAGAGTACATGGGGG13570.10578356231018204No Hit
CATCTAAGTACCCCGAGGAAAAGAA13470.1050040224258034No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13400.10445834450673835No Hit
CACACACACTGATTCAGGCTCTGGG13290.10360085063392183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCC359.882011E-519.013973
ACTAATC654.9112714E-1018.9932178
TACTAAT651.0066287E-817.53227
CTCCTAG555.7979923E-717.3036581
CTTATAC1750.016.3148771
CTATAGT350.00215929416.2976884
CGCATGA350.002175102716.27990216
CCCTTAA551.1090349E-515.5732921
CTAGGCC551.120171E-515.5568844
GTCTGGC456.6700875E-414.8042391
TCGTTCG456.7795155E-414.7725039
CCTATAT653.309E-614.6415551
TAAGGCC400.005252761814.2604764
CCGTCGC400.005290955314.24491310
GGTATGC602.5762294E-514.2449138
ACGAAGC400.005290955314.2449138
TATACAC2100.014.03412153
TATACTA954.8476068E-914.0102935
ACCCTAG500.001479218413.3238161
TTAGGTC500.001491016613.3097794
Produced by FastQC (version 0.11.5)