FastQCFastQC Report
Wed 25 May 2016
SRR1295260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA500424.231112313617397No Hit
GCTTATGTACTCTGCGTTGATACCA293332.4801410314403727No Hit
CTTATGTACTCTGCGTTGATACCAC209501.771348126979027No Hit
GAGTACATAAGCAGTGGTATCAACG130081.0998423119686485No Hit
TATGTACTCTGCGTTGATACCACTG123191.0415865191529659No Hit
GCGTTGATACCACTGCTTATGTACT94590.799770020672774No Hit
CATAAGCAGTGGTATCAACGCAGAG82370.6964484258676013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78550.6641498585880791No Hit
CTGCTTATGTACTCTGCGTTGATAC76610.6477469212785836No Hit
ACGCAGAGTACATAAGCAGTGGTAT68860.5822197232638463No Hit
ACTCTGCGTTGATACCACTGCTTAT65670.5552478830487481No Hit
GTACTGGTTCACTATCGGTCAGTCA65040.5499211559843242No Hit
GTATCAACGCAGAGTACATAAGCAG57690.4877760068993798No Hit
GTATCAACGCAGAGTACTTTTTTTT56890.48101190903979407No Hit
TATCAACGCAGAGTACATAAGCAGT52940.4476141758580892No Hit
CCCATGTACTCTGCGTTGATACCAC52260.44186469267744133No Hit
GGTATCAACGCAGAGTACATAAGCA51790.4378907851849347No Hit
GGATACCACGTGTCCCGCCCTACTC48120.4068604862540849No Hit
GGTATCAACGCAGAGTACTTTTTTT43950.371602626160994No Hit
GTGGTATCAACGCAGAGTACATGGG41710.3526631521541538No Hit
ATACAGGGTGACAGCCCCGTACACA40810.3450535420621198No Hit
GTGGTATCAACGCAGAGTACATAAG38650.32679047784123816No Hit
TATCAACGCAGAGTACTTTTTTTTT34680.29322364221304376No Hit
CATGTACTCTGCGTTGATACCACTG34540.2920399250876162No Hit
GTACATGATAAGCAGTGGTATCAAC33910.2867131980231924No Hit
ACATAAGCAGTGGTATCAACGCAGA33620.28426121254909253No Hit
ATCATGTACTCTGCGTTGATACCAC33590.2840075588793581No Hit
AAGCAGTGGTATCAACGCAGAGTAC33310.2816401246285031No Hit
ACCCTGTATCGCGCGCCTTTCCAGA29970.25340001606473245No Hit
GTATTTAGCCTTGGAGGATGGTCCC28920.24452213762402608No Hit
GATACCACTGCTTATGTACTCTGCG28170.2381807958806644No Hit
CCCATATTCAGACAGGATACCACGT27350.23124759557458896No Hit
CCACTGCTTATGTACTCTGCGTTGA26720.22592086851016516No Hit
GTTGATACCACTGCTTATGTACTCT25800.21814215597164155No Hit
GTTCACTATCGGTCAGTCAGGAGTA24380.20613588227087676No Hit
TCTAAGTACCCCGAGGAAAAGAAAT24180.20444485780598032No Hit
CTATCGGTCAGTCAGGAGTATTTAG23860.20173921866214597No Hit
GGTTAATGAGGCGAACCGGGGGAAC23550.19911813074155651No Hit
GCCTTGGAGGATGGTCCCCCCATAT23540.19903357951831166No Hit
TATTCAGACAGGATACCACGTGTCC23220.19632794037447737No Hit
ATACCACTGCTTATGTACTCTGCGT22880.1934531987841534No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG22380.18922563762191227No Hit
GCCCAGAGCCTGAATCAGTGTGTGT21750.18389891055748847No Hit
GTCAGGAGTATTTAGCCTTGGAGGA21720.18364525688775402No Hit
GCAGTGGTATCAACGCAGAGTACAT21660.18313794954828508No Hit
GTACTCTGCGTTGATACCACTGCTT21340.1804323104044508No Hit
ATCATTAACTGAATCCATAGGTTAA20960.1772193639211475No Hit
GGTATCAACGCAGAGTACATGGGGG20330.17189263685672374No Hit
GTTAATGATAGTGTGTCGAAACACA19290.1630993096392622No Hit
GTACATGGGAAGCAGTGGTATCAAC18830.15920995337000038No Hit
TACCACTGCTTATGTACTCTGCGTT18500.15641976300292124No Hit
CATCTAAGTACCCCGAGGAAAAGAA18070.1527840604033939No Hit
CAGTGGTATCAACGCAGAGTACATA17690.14957111392009065No Hit
TGATACCACTGCTTATGTACTCTGC17270.1460199625438081No Hit
CACACACACTGATTCAGGCTCTGGG16720.14136964526534287No Hit
GCAGAGTACATAAGCAGTGGTATCA16330.1380721475587948No Hit
CGGTACTGGTTCACTATCGGTCAGT16300.13781849388906034No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15690.1326608692711262No Hit
GCCTGAATCAGTGTGTGTGTTAGTG15490.13096984480622972No Hit
TCACGGTACTGGTTCACTATCGGTC15480.1308852935829849No Hit
ACTTAGATGTTTCAGTTCCCCCGGT15060.1273341422067024No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14870.12572766896505075No Hit
GAATCAGTGTGTGTGTTAGTGGAAG14780.12496670795584736No Hit
GAACAAAAAAAAAAAAAAAAAAAAA14730.12454395183962325No Hit
GTCCCGCCCTACTCATCGAGCTCAC14550.12302202982121643No Hit
GGTTCACTATCGGTCAGTCAGGAGT14320.12107735168658552No Hit
CCCCAGTAGCGGCGAGCGAACGGGG14180.11989363456115802No Hit
GTATCAACGCAGAGTACATGGGGGT13840.11701889297083407No Hit
GGAGTATTTAGCCTTGGAGGATGGT13760.11634248318487547No Hit
GGTATCAACGCAGAGTACATGGGGA12980.10974748777177934No Hit
GCCCTACTCATCGAGCTCACAGCAT12920.1092401804323104No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG12300.10399800459113143No Hit
GTACACAAGCAGTGGTATCAACGCA12130.10256063379596944No Hit
GTACATGGGGGTTAAGCGACTAAGC12030.10171512156352122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA307.7082956E-419.004742
GGCCCCA250.006035819618.99750511
TTGCGAC453.5186604E-516.89953619
TATAGAG453.5301724E-516.89312
ACGGCTT453.5417175E-516.88667112
GCTATTT402.7631834E-416.62281613
CGTAGAT402.7631834E-416.62281614
ACTGGCC402.7640752E-416.6221128
GTCCTAC350.002155789616.3014981
TGACGCG350.002160671916.29597919
GCCGCAT350.002160671916.29597919
CTCCGGA350.002171691316.28357511
TCGCTTG350.002171691316.28357513
CGAGCCG350.002171691316.28357510
TGCTATT350.002171691316.28357512
CTTCTAA755.772199E-815.2147331
TTGTATC1500.015.19800413
ACACCGT508.72404E-515.1973616
AGTTGTA1450.015.06698611
CCTCTGG456.7111535E-414.7921011
Produced by FastQC (version 0.11.5)