##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295260_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1182715 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.292784821364403 31.0 30.0 33.0 23.0 34.0 2 29.134187018850696 31.0 28.0 33.0 18.0 34.0 3 29.57380518552652 31.0 30.0 34.0 23.0 34.0 4 32.64429554034573 35.0 33.0 37.0 20.0 37.0 5 33.24335786727995 35.0 33.0 37.0 27.0 37.0 6 33.32093361460707 35.0 33.0 37.0 26.0 37.0 7 33.38605919431139 35.0 33.0 37.0 26.0 37.0 8 33.412828111590706 36.0 33.0 37.0 26.0 37.0 9 34.09425516713663 38.0 33.0 39.0 21.0 39.0 10 34.0655652460652 38.0 33.0 39.0 22.0 39.0 11 34.50240336852073 38.0 34.0 39.0 25.0 39.0 12 34.51932037726756 38.0 34.0 39.0 25.0 39.0 13 34.259649196974756 38.0 34.0 39.0 23.0 39.0 14 34.300711498543606 38.0 32.0 40.0 17.0 41.0 15 34.89152078057689 38.0 33.0 40.0 22.0 41.0 16 34.862328625239385 38.0 33.0 40.0 22.0 41.0 17 34.913434766617485 38.0 33.0 40.0 23.0 41.0 18 34.84146307436703 38.0 33.0 40.0 22.0 41.0 19 34.935815475410394 39.0 33.0 40.0 22.0 41.0 20 35.010783663012646 39.0 33.0 40.0 22.0 41.0 21 35.01461467893787 39.0 33.0 40.0 23.0 41.0 22 34.77302224119927 39.0 33.0 40.0 22.0 41.0 23 34.44920458436732 38.0 32.0 40.0 19.0 41.0 24 34.782533408302086 38.0 32.0 40.0 23.0 41.0 25 33.5826171140131 38.0 30.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5887300450721256 1101 2 0.8581663132609236 1101 3 0.6808488378472077 1101 4 0.7707537757149865 1101 5 0.45482519009901523 1101 6 0.37292683293870255 1101 7 0.45107395292037467 1101 8 0.5349667403523881 1101 9 0.6307788100860563 1101 10 0.850325279027544 1101 11 0.7962241007300008 1101 12 0.8155763388797084 1101 13 0.8820679147738986 1101 14 1.4050296523831065 1101 15 1.2642721055410817 1101 16 0.972708359636556 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2210 9 0.7858878692114715 2210 10 0.6708253051435022 2210 11 0.17929295906260023 2210 12 0.3758464112153561 2210 13 0.25962269438300467 2210 14 -1.817796416454108 2210 15 -0.5517982097285312 2210 16 0.08795168812084597 2210 17 0.3428576094811717 2210 18 0.4179433909775483 2210 19 0.6415247137580593 2210 20 0.7464951507551092 2210 21 0.7866790340914349 2210 22 0.9099571177025467 2210 23 1.346072849919672 2210 24 1.1023851050860216 2210 25 0.8008472962315878 2211 1 -0.02612951950049691 2211 2 -0.6831904722847888 2211 3 -0.10508407495908045 2211 4 0.29473229781497423 2211 5 0.28924071292541953 2211 6 0.10449157963494571 2211 7 0.129016200936114 2211 8 0.2910659819084671 2211 9 0.738523429731508 2211 10 0.7422283294294161 2211 11 0.2683306154028813 2211 12 0.1235819002810814 2211 13 -0.10387096783522765 2211 14 -0.9039241034039733 2211 15 -0.21472813659858758 2211 16 0.07444244788028698 2211 17 0.2629202116350555 2211 18 0.25288265481952266 2211 19 0.5950812293468317 2211 20 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0.053672545222802626 2213 7 -0.046885976504214 2213 8 -0.045829568276666066 2213 9 0.5971830532598759 2213 10 0.5989890165838716 2213 11 -0.22956057506419114 2213 12 0.029872401013747663 2213 13 -0.22743208955057526 2213 14 -1.8692847022756496 2213 15 -0.669676960804999 2213 16 -0.2992123987031903 2213 17 -2.0303731189839525 2213 18 -0.8483223144125205 2213 19 -0.397435142271263 2213 20 0.14434939148644332 2213 21 -0.13662125075851606 2213 22 0.3704176251329372 2213 23 0.697851506046149 2213 24 0.6319879712507088 2213 25 -0.9843890850698855 2214 1 0.36589211254040777 2214 2 0.315679435915289 2214 3 0.3198114065772444 2214 4 0.5463477880874663 2214 5 0.6986582948696025 2214 6 0.534760006493066 2214 7 0.42699425832032745 2214 8 0.2725910347813354 2214 9 0.7399074916300776 2214 10 0.8881982697890223 2214 11 0.26823017949601535 2214 12 0.19646756164026158 2214 13 0.0356137183188423 2214 14 1.0597243599016082 2214 15 0.6397154384588433 2214 16 0.6498953612228817 2214 17 0.7123157928558967 2214 18 0.44721862069235385 2214 19 -0.511835423377363 2214 20 0.3971400683930284 2214 21 0.8117468584535317 2214 22 1.20329636124265 2214 23 1.4979303244686974 2214 24 1.302281385447337 2214 25 1.4500673784290257 2215 1 -0.03428610251352282 2215 2 0.26707991892079264 2215 3 0.08850942718651567 2215 4 0.46259933145312715 2215 5 0.36436043888600267 2215 6 0.2853919128117397 2215 7 0.3493590827843249 2215 8 0.255059759786576 2215 9 0.4464913536551265 2215 10 0.7906886213269502 2215 11 0.5910915988488483 2215 12 0.4280937448872706 2215 13 0.17671810807788546 2215 14 1.1825927680800845 2215 15 0.5709356874163447 2215 16 0.422625545960841 2215 17 0.6880063276563604 2215 18 0.6501954721487451 2215 19 -0.03626913436612256 2215 20 -1.3348996203712673 2215 21 -1.5358307369245239 2215 22 0.26122790181278077 2215 23 0.9023969605916093 2215 24 1.055901014728967 2215 25 0.2957492548510601 2216 1 -0.18529894836332517 2216 2 -0.3877228643466637 2216 3 -0.3313172361863437 2216 4 -0.15985002625438227 2216 5 0.14970917077005907 2216 6 -0.09317569259010838 2216 7 -0.21135196794030975 2216 8 -0.46109224943608496 2216 9 -0.11223723659519891 2216 10 -1.9688201896084934 2216 11 -0.8163750513487784 2216 12 -0.5966695126884929 2216 13 -0.29628502103937393 2216 14 -0.8869971528685312 2216 15 -0.5792248857061892 2216 16 -0.2308041312460105 2216 17 -5.085768232149235E-4 2216 18 -0.15643949161936632 2216 19 -1.3318286699392488 2216 20 -0.973832426695374 2216 21 -0.7468320544475802 2216 22 -0.8037609815864286 2216 23 -4.205963134928815 2216 24 -1.9821877533869738 2216 25 -1.414317444753685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 17.0 5 21.0 6 32.0 7 78.0 8 41.0 9 138.0 10 469.0 11 1210.0 12 2869.0 13 5135.0 14 8005.0 15 11196.0 16 14216.0 17 15658.0 18 15530.0 19 14461.0 20 13621.0 21 12610.0 22 11739.0 23 11397.0 24 11437.0 25 11886.0 26 12694.0 27 14350.0 28 16372.0 29 19747.0 30 24317.0 31 29892.0 32 39962.0 33 53549.0 34 72396.0 35 99321.0 36 140307.0 37 205410.0 38 292627.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.95757966558942 18.985361180854028 12.065707557029112 19.991351596527437 2 13.312106758676679 24.26721805019958 39.90570665042961 22.514968540694134 3 19.908741452440257 32.65093817000816 30.323464610882517 17.11685576666906 4 11.935499969127005 21.81485845366048 32.51822986932687 33.73141170788564 5 10.28397411496003 45.945353804508734 30.918834327285033 12.851837753246203 6 26.64774668421106 35.07412957369153 23.937104024950024 14.341019717147391 7 22.389819242160026 40.16293068891621 19.79295810619502 17.65429196272874 8 20.85999786852883 39.81774229836875 23.73604605331046 15.58621377979196 9 27.42768887757372 23.467652101393316 17.554874577830933 31.549784443202032 10 15.312560257368737 30.319224510921593 27.30195834334099 27.066256888368677 11 27.62790241394241 31.733143672872917 19.557381873782205 21.08157203940247 12 24.46776933725602 29.921109356858178 23.896448525736922 21.714672780148884 13 27.252991302641682 24.417046957616023 30.430859215106544 17.899102524635747 14 27.42177092276266 28.034323945985033 23.181689384359682 21.36221574689262 15 30.24289076977859 27.061805749973995 23.201705826181318 19.493597654066097 16 22.8573482401096 27.78918525785227 27.8590396292475 21.494426872790623 17 19.46156272648777 35.47116644213457 24.994016705864645 20.07325412551302 18 19.790054482316506 26.412506068156176 35.113523150332966 18.683916299194355 19 21.718812631393796 34.19392920506649 22.983491104545063 21.10376705899465 20 20.779593079303872 31.16139834593333 28.836910379352922 19.22209819540988 21 22.82255745911482 32.76437957685738 24.081499771685635 20.331563192342166 22 20.980335612980372 31.012784008023726 22.003512917201203 26.003367461794703 23 25.63249310995406 26.458960357817276 23.31985627097775 24.588690261250925 24 19.74609768101872 29.44384026411795 22.214338749532768 28.595723305330562 25 23.374422618377515 32.60389483422789 23.475318919495418 20.54636362789918 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8902.0 1 8902.0 2 7431.5 3 5961.0 4 5961.0 5 5961.0 6 7604.0 7 9247.0 8 9247.0 9 9247.0 10 9442.0 11 9637.0 12 9637.0 13 9637.0 14 11749.0 15 13861.0 16 13861.0 17 13861.0 18 17611.5 19 21362.0 20 21362.0 21 21362.0 22 27601.5 23 33841.0 24 33841.0 25 33841.0 26 40607.5 27 47374.0 28 47374.0 29 47374.0 30 51287.0 31 55200.0 32 55200.0 33 55200.0 34 59416.0 35 63632.0 36 63632.0 37 63632.0 38 78335.5 39 93039.0 40 93039.0 41 93039.0 42 194216.0 43 295393.0 44 295393.0 45 295393.0 46 239395.5 47 183398.0 48 183398.0 49 183398.0 50 156061.0 51 128724.0 52 128724.0 53 128724.0 54 104296.5 55 79869.0 56 79869.0 57 79869.0 58 66767.0 59 53665.0 60 53665.0 61 53665.0 62 49585.0 63 45505.0 64 45505.0 65 45505.0 66 31377.5 67 17250.0 68 17250.0 69 17250.0 70 13918.0 71 10586.0 72 10586.0 73 10586.0 74 7717.5 75 4849.0 76 4849.0 77 4849.0 78 2986.5 79 1124.0 80 1124.0 81 1124.0 82 668.5 83 213.0 84 213.0 85 213.0 86 135.5 87 58.0 88 58.0 89 58.0 90 38.5 91 19.0 92 19.0 93 19.0 94 10.5 95 2.0 96 2.0 97 2.0 98 3.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08404391590535336 2 0.01986953746253324 3 0.030438440368136026 4 0.03821715290665968 5 0.0477714411333246 6 0.016149283639761057 7 0.02105325458796075 8 0.036610679665008056 9 0.03618792354878394 10 0.024266201071264 11 0.021222357034450395 12 0.01623383486300588 13 0.024266201071264 14 0.038048050460170034 15 0.03500420642335643 16 0.021560561927429688 17 0.021645113150674508 18 0.026464532875629377 19 0.01699479587220928 20 0.014796464067843901 21 0.011414415138051009 22 0.018770371560350548 23 0.017924859327902325 24 0.01800941055114715 25 0.11025479511124828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1182715.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.289482427330984 #Duplication Level Percentage of deduplicated Percentage of total 1 79.83239531245799 16.99590377136315 2 7.147795884122638 3.043457497383553 3 2.9462492846534802 1.8817236711650027 4 1.7445266748309998 1.4856027995129892 5 1.1768385278771973 1.252714157952383 6 0.8116561873844226 1.0367844083013078 7 0.6646160402057298 0.9904532056217533 8 0.5411995845985259 0.9217487236791315 9 0.4297137989310506 0.8233545934011819 >10 3.8322975050209456 16.744923363661073 >50 0.49529039465339586 7.339817209626711 >100 0.30677842530891564 12.613998571217433 >500 0.03611492458358515 5.31583542611703 >1k 0.027780711218142424 12.150396482987027 >5k 0.004762407637395844 6.786823644832421 >10k+ 0.001984336515581602 10.616462473177842 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 50042 4.231112313617397 No Hit GCTTATGTACTCTGCGTTGATACCA 29333 2.4801410314403727 No Hit CTTATGTACTCTGCGTTGATACCAC 20950 1.771348126979027 No Hit GAGTACATAAGCAGTGGTATCAACG 13008 1.0998423119686485 No Hit TATGTACTCTGCGTTGATACCACTG 12319 1.0415865191529659 No Hit GCGTTGATACCACTGCTTATGTACT 9459 0.799770020672774 No Hit CATAAGCAGTGGTATCAACGCAGAG 8237 0.6964484258676013 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 7855 0.6641498585880791 No Hit CTGCTTATGTACTCTGCGTTGATAC 7661 0.6477469212785836 No Hit ACGCAGAGTACATAAGCAGTGGTAT 6886 0.5822197232638463 No Hit ACTCTGCGTTGATACCACTGCTTAT 6567 0.5552478830487481 No Hit GTACTGGTTCACTATCGGTCAGTCA 6504 0.5499211559843242 No Hit GTATCAACGCAGAGTACATAAGCAG 5769 0.4877760068993798 No Hit GTATCAACGCAGAGTACTTTTTTTT 5689 0.48101190903979407 No Hit TATCAACGCAGAGTACATAAGCAGT 5294 0.4476141758580892 No Hit CCCATGTACTCTGCGTTGATACCAC 5226 0.44186469267744133 No Hit GGTATCAACGCAGAGTACATAAGCA 5179 0.4378907851849347 No Hit GGATACCACGTGTCCCGCCCTACTC 4812 0.4068604862540849 No Hit GGTATCAACGCAGAGTACTTTTTTT 4395 0.371602626160994 No Hit GTGGTATCAACGCAGAGTACATGGG 4171 0.3526631521541538 No Hit ATACAGGGTGACAGCCCCGTACACA 4081 0.3450535420621198 No Hit GTGGTATCAACGCAGAGTACATAAG 3865 0.32679047784123816 No Hit TATCAACGCAGAGTACTTTTTTTTT 3468 0.29322364221304376 No Hit CATGTACTCTGCGTTGATACCACTG 3454 0.2920399250876162 No Hit GTACATGATAAGCAGTGGTATCAAC 3391 0.2867131980231924 No Hit ACATAAGCAGTGGTATCAACGCAGA 3362 0.28426121254909253 No Hit ATCATGTACTCTGCGTTGATACCAC 3359 0.2840075588793581 No Hit AAGCAGTGGTATCAACGCAGAGTAC 3331 0.2816401246285031 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 2997 0.25340001606473245 No Hit GTATTTAGCCTTGGAGGATGGTCCC 2892 0.24452213762402608 No Hit GATACCACTGCTTATGTACTCTGCG 2817 0.2381807958806644 No Hit CCCATATTCAGACAGGATACCACGT 2735 0.23124759557458896 No Hit CCACTGCTTATGTACTCTGCGTTGA 2672 0.22592086851016516 No Hit GTTGATACCACTGCTTATGTACTCT 2580 0.21814215597164155 No Hit GTTCACTATCGGTCAGTCAGGAGTA 2438 0.20613588227087676 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 2418 0.20444485780598032 No Hit CTATCGGTCAGTCAGGAGTATTTAG 2386 0.20173921866214597 No Hit GGTTAATGAGGCGAACCGGGGGAAC 2355 0.19911813074155651 No Hit GCCTTGGAGGATGGTCCCCCCATAT 2354 0.19903357951831166 No Hit TATTCAGACAGGATACCACGTGTCC 2322 0.19632794037447737 No Hit ATACCACTGCTTATGTACTCTGCGT 2288 0.1934531987841534 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 2238 0.18922563762191227 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 2175 0.18389891055748847 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 2172 0.18364525688775402 No Hit GCAGTGGTATCAACGCAGAGTACAT 2166 0.18313794954828508 No Hit GTACTCTGCGTTGATACCACTGCTT 2134 0.1804323104044508 No Hit ATCATTAACTGAATCCATAGGTTAA 2096 0.1772193639211475 No Hit GGTATCAACGCAGAGTACATGGGGG 2033 0.17189263685672374 No Hit GTTAATGATAGTGTGTCGAAACACA 1929 0.1630993096392622 No Hit GTACATGGGAAGCAGTGGTATCAAC 1883 0.15920995337000038 No Hit TACCACTGCTTATGTACTCTGCGTT 1850 0.15641976300292124 No Hit CATCTAAGTACCCCGAGGAAAAGAA 1807 0.1527840604033939 No Hit CAGTGGTATCAACGCAGAGTACATA 1769 0.14957111392009065 No Hit TGATACCACTGCTTATGTACTCTGC 1727 0.1460199625438081 No Hit CACACACACTGATTCAGGCTCTGGG 1672 0.14136964526534287 No Hit GCAGAGTACATAAGCAGTGGTATCA 1633 0.1380721475587948 No Hit CGGTACTGGTTCACTATCGGTCAGT 1630 0.13781849388906034 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1569 0.1326608692711262 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 1549 0.13096984480622972 No Hit TCACGGTACTGGTTCACTATCGGTC 1548 0.1308852935829849 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1506 0.1273341422067024 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1487 0.12572766896505075 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 1478 0.12496670795584736 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 1473 0.12454395183962325 No Hit GTCCCGCCCTACTCATCGAGCTCAC 1455 0.12302202982121643 No Hit GGTTCACTATCGGTCAGTCAGGAGT 1432 0.12107735168658552 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 1418 0.11989363456115802 No Hit GTATCAACGCAGAGTACATGGGGGT 1384 0.11701889297083407 No Hit GGAGTATTTAGCCTTGGAGGATGGT 1376 0.11634248318487547 No Hit GGTATCAACGCAGAGTACATGGGGA 1298 0.10974748777177934 No Hit GCCCTACTCATCGAGCTCACAGCAT 1292 0.1092401804323104 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 1230 0.10399800459113143 No Hit GTACACAAGCAGTGGTATCAACGCA 1213 0.10256063379596944 No Hit GTACATGGGGGTTAAGCGACTAAGC 1203 0.10171512156352122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.691024464896446E-4 0.0 0.0 0.0 3 0.0 1.691024464896446E-4 0.0 0.0 0.0 4 0.0 1.691024464896446E-4 0.0 0.0 0.0 5 0.0 1.691024464896446E-4 0.0 0.0 0.0 6 8.45512232448223E-5 1.691024464896446E-4 0.0 0.0 0.0 7 8.45512232448223E-5 2.536536697344669E-4 0.0 0.0 0.0 8 8.45512232448223E-5 2.536536697344669E-4 0.0 0.0 0.0 9 8.45512232448223E-5 2.536536697344669E-4 0.0 0.0 0.0 10 8.45512232448223E-5 2.536536697344669E-4 0.0 0.0 0.0 11 8.45512232448223E-5 2.536536697344669E-4 0.0 0.0 0.0 12 8.45512232448223E-5 4.227561162241115E-4 0.0 0.0 0.0 13 8.45512232448223E-5 4.227561162241115E-4 0.0 1.691024464896446E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTA 30 7.7082956E-4 19.00474 2 GGCCCCA 25 0.0060358196 18.997505 11 TTGCGAC 45 3.5186604E-5 16.899536 19 TATAGAG 45 3.5301724E-5 16.8931 2 ACGGCTT 45 3.5417175E-5 16.886671 12 GCTATTT 40 2.7631834E-4 16.622816 13 CGTAGAT 40 2.7631834E-4 16.622816 14 ACTGGCC 40 2.7640752E-4 16.622112 8 GTCCTAC 35 0.0021557896 16.301498 1 TGACGCG 35 0.0021606719 16.295979 19 GCCGCAT 35 0.0021606719 16.295979 19 CTCCGGA 35 0.0021716913 16.283575 11 TCGCTTG 35 0.0021716913 16.283575 13 CGAGCCG 35 0.0021716913 16.283575 10 TGCTATT 35 0.0021716913 16.283575 12 CTTCTAA 75 5.772199E-8 15.214733 1 TTGTATC 150 0.0 15.198004 13 ACACCGT 50 8.72404E-5 15.197361 6 AGTTGTA 145 0.0 15.066986 11 CCTCTGG 45 6.7111535E-4 14.792101 1 >>END_MODULE