FastQCFastQC Report
Wed 25 May 2016
SRR1295260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA552734.673399762411062No Hit
GCTTATGTACTCTGCGTTGATACCA322182.7240713105016847No Hit
CTTATGTACTCTGCGTTGATACCAC241192.039290953441869No Hit
GTATCAACGCAGAGTACTTTTTTTT189001.5980181193271412No Hit
GAGTACATAAGCAGTGGTATCAACG151071.2773153295595303No Hit
GGTATCAACGCAGAGTACTTTTTTT148201.2530491284882663No Hit
TATCAACGCAGAGTACTTTTTTTTT132851.1232630008074642No Hit
TATGTACTCTGCGTTGATACCACTG118511.0020165466743889No Hit
CATAAGCAGTGGTATCAACGCAGAG109900.929217943460597No Hit
GCGTTGATACCACTGCTTATGTACT104450.883137526792169No Hit
ACGCAGAGTACATAAGCAGTGGTAT85940.7266332125660028No Hit
CTGCTTATGTACTCTGCGTTGATAC84340.7131050168468313No Hit
GTACTTTTTTTTTTTTTTTTTTTTT80910.6841039472738571No Hit
ACTCTGCGTTGATACCACTGCTTAT72720.6148564954363478No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71580.605217655986438No Hit
GTACTGGTTCACTATCGGTCAGTCA68430.578584020664319No Hit
TATCAACGCAGAGTACATAAGCAGT64580.5460317997150623No Hit
GTATCAACGCAGAGTACATAAGCAG64250.5432416093479833No Hit
CCCATGTACTCTGCGTTGATACCAC59840.5059545198970167No Hit
GGTATCAACGCAGAGTACATAAGCA59700.5047708027715891No Hit
GGATACCACGTGTCCCGCCCTACTC54230.45852128365667133No Hit
ATACAGGGTGACAGCCCCGTACACA45900.38809011469373433No Hit
GTGGTATCAACGCAGAGTACATAAG45220.38234063151308645No Hit
ACATAAGCAGTGGTATCAACGCAGA44010.37210993350046295No Hit
GTGGTATCAACGCAGAGTACATGGG43980.37185627983072844No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42140.35629885475368117No Hit
AAGCAGTGGTATCAACGCAGAGTAC39950.3377821368630651No Hit
CATGTACTCTGCGTTGATACCACTG39580.33465374160300665No Hit
ATCATGTACTCTGCGTTGATACCAC36420.3079355550576428No Hit
ACCCTGTATCGCGCGCCTTTCCAGA35050.2963520374731021No Hit
GTACATGATAAGCAGTGGTATCAAC34130.2885733249345785No Hit
CCCATATTCAGACAGGATACCACGT32780.27715890979652746No Hit
GATACCACTGCTTATGTACTCTGCG31990.2704793631601865No Hit
CCACTGCTTATGTACTCTGCGTTGA31120.263123406737887No Hit
GTATTTAGCCTTGGAGGATGGTCCC31020.26227789450543876No Hit
CTATCGGTCAGTCAGGAGTATTTAG29440.24891880123275684No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29050.24562130352620876No Hit
TATTCAGACAGGATACCACGTGTCC27830.23530605429034046No Hit
TCTAAGTACCCCGAGGAAAAGAAAT27800.23505240062060595No Hit
GGTTAATGAGGCGAACCGGGGGAAC26460.2237225367057998No Hit
ATCATTAACTGAATCCATAGGTTAA26440.22355343425931015No Hit
GTCAGGAGTATTTAGCCTTGGAGGA25760.21780395107866224No Hit
GCAGTGGTATCAACGCAGAGTACAT25750.21771939985541744No Hit
GCCTTGGAGGATGGTCCCCCCATAT25620.2166202339532347No Hit
GTTGATACCACTGCTTATGTACTCT25350.21433735092562453No Hit
ATACCACTGCTTATGTACTCTGCGT25190.21298453135370735No Hit
GCCCAGAGCCTGAATCAGTGTGTGT24990.2112935068888109No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG24680.20867241896822142No Hit
GGTATCAACGCAGAGTACATGGGGG24000.2029229357875735No Hit
GTTCACTATCGGTCAGTCAGGAGTA23980.20275383334108385No Hit
CAGTGGTATCAACGCAGAGTACATA23240.19649704282096703No Hit
GTTAATGATAGTGTGTCGAAACACA21660.18313794954828508No Hit
GTACTCTGCGTTGATACCACTGCTT21540.18212333486934723No Hit
CATCTAAGTACCCCGAGGAAAAGAA21480.1816160275298783No Hit
CACACACACTGATTCAGGCTCTGGG21350.1805168616276956No Hit
CGGTACTGGTTCACTATCGGTCAGT20470.17307635398215124No Hit
TACCACTGCTTATGTACTCTGCGTT20400.1724844954194375No Hit
GCAGAGTACATAAGCAGTGGTATCA20340.17197718807996856No Hit
GTGGTATCAACGCAGAGTACTTTTT19950.16867969037342048No Hit
TGATACCACTGCTTATGTACTCTGC19670.16631225612256545No Hit
GTACATGGGAAGCAGTGGTATCAAC19470.164621231657669No Hit
GCCTGAATCAGTGTGTGTGTTAGTG19270.16293020719277257No Hit
GAATCAGTGTGTGTGTTAGTGGAAG17960.15185399694770085No Hit
TCACGGTACTGGTTCACTATCGGTC17790.15041662615253887No Hit
ACTTAGATGTTTCAGTTCCCCCGGT17590.14872560168764243No Hit
CCCCAGTAGCGGCGAGCGAACGGGG17550.14838739679466312No Hit
GGTATCAACGCAGAGTACATGGGGA15940.13477464985224674No Hit
ATCAACGCAGAGTACTTTTTTTTTT15890.13435189373602263No Hit
GGAGTATTTAGCCTTGGAGGATGGT15800.13359093272681924No Hit
GGTTCACTATCGGTCAGTCAGGAGT15610.1319844594851676No Hit
GTATCAACGCAGAGTACATGGGGGT15550.13147715214569866No Hit
GTCCCGCCCTACTCATCGAGCTCAC14730.12454395183962325No Hit
GCCCTACTCATCGAGCTCACAGCAT14170.1198090833379132No Hit
AGCTCACAGCATGTGCATTTTTGTG13820.11684979052434441No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG13810.11676523930109958No Hit
ATCAACGCAGAGTACATAAGCAGTG13770.1164270344081203No Hit
GTACACAAGCAGTGGTATCAACGCA13450.11372139526428597No Hit
GAGTACATGATAAGCAGTGGTATCA13110.11084665367396201No Hit
CAGTAGCGGCGAGCGAACGGGGAGC12550.10611178517225199No Hit
CCCCATGTACTCTGCGTTGATACCA12390.10475896560033482No Hit
GGTATCAACGCAGAGTACATGGGCA12230.10340614602841766No Hit
TACATAAGCAGTGGTATCAACGCAG12070.1020533264565005No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA11830.10002409709862477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTC401.2780667E-519.0115533
TTAAGAT307.6897326E-419.0115534
GCTCTCA359.964952E-518.99306716
CCTTTTC250.006043970618.9930678
AGCTCTC359.964952E-518.99306715
ATCGGTA451.6735521E-618.99306714
CTGATCT250.006043970618.9930679
GGTTCTA504.5763227E-617.093768
CCTTATA804.274625E-1016.6351092
AGGGCGC402.7609777E-416.62455619
GTATCCG402.7681116E-416.61893516
GTTGGGC402.7681116E-416.6189359
TTAGGGC350.002160993916.2956163
GGATCGT350.002175083416.27977210
TCTCACT551.121374E-515.5549062
CCGTGTC551.1317614E-515.5397829
CCCTTAT755.6756107E-815.2356771
TACAATC508.666077E-515.2092424
CACCTAG508.738291E-515.19445318
CTAGAAT456.7295483E-414.7867633
Produced by FastQC (version 0.11.5)