FastQCFastQC Report
Wed 25 May 2016
SRR1295259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4894179
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG67100.137101646670463No Hit
GTATCAACGCAGAGTACTTTTTTTT61040.12471959035417379No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN54080.11049861478299017No Hit
GGTATCAACGCAGAGTACTTTTTTT50670.1035311540505568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG550.003056967912.0978125
GGTATCA36700.010.464381
TGGTATC17600.010.423242
GTGGTAT18100.010.1887621
TGCACCG1554.2313513E-79.1987975
AATACCG950.00181546069.0051385
GTCTAGG3650.08.8549131
TAGACTG6250.08.6689475
TATTCCG2659.094947E-128.60868555
CTAGACT3500.08.41900354
TACACCG1252.7285592E-48.3647735
CGTAGTA1050.004493528.1472562
GCGTGGG1304.1935037E-48.0376079
TACACGG1200.00168244097.9211875
AACGTGG1200.00169387427.9157446
CCGTCCA1100.00686372357.77190079
GGACGGT1100.0068643547.77182056
GGGGCGT1356.286163E-47.73983866
GTCTAAT5800.07.70314551
CTAATAC6800.07.6879743
Produced by FastQC (version 0.11.5)