FastQCFastQC Report
Wed 25 May 2016
SRR1295259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4894179
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT175140.35785368700245745No Hit
GGTATCAACGCAGAGTACTTTTTTT149290.30503583951465607No Hit
TATCAACGCAGAGTACTTTTTTTTT95480.19508890050813427No Hit
GTGGTATCAACGCAGAGTACATGGG72100.14731786475320988No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62630.1279683477044873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA5250.012.6675383
GGTATCA49700.012.25536251
CAGCGTC5600.011.8755752
GCAGCGT5250.011.2216541
GTGGTAT20050.010.758111
TACGCTG909.5180905E-510.5561745
TGGTATC19500.010.1338242
TCTAGAC5050.09.594723
GCGTCAG7150.09.3011494
GTCTACG1054.4854102E-49.0497211
CTAGACT5000.08.7404234
ACCGAGT1201.7650856E-48.7083998
GTGTTAG8200.08.6910431
TAACGCA1107.1458006E-48.6367824
CGTCTAT1000.00290060428.5519861
TAGAGCG1000.00290516938.5505015
CGTCAGA7900.08.4182155
TAGACAG6550.08.4127065
CTACACT7450.08.2889924
TGCGCTT1150.00111123138.26093212
Produced by FastQC (version 0.11.5)