FastQCFastQC Report
Wed 25 May 2016
SRR1295258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350491
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG63570.14612143778713713No Hit
GTATCAACGCAGAGTACTTTTTTTT59620.13704200284519608No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN50050.11504448578332882No Hit
GGTATCAACGCAGAGTACTTTTTTT49590.11398713386603948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34750.011.13326551
GTGGTAT16250.010.9973341
TGGTATC15050.010.736442
ACCGACC750.002652570610.13178358
AGCACCG1304.2123356E-69.5054555
CGCAACC800.0045316649.49657318
ACCGTGG1951.6898412E-99.2549948
TGTAGCG1201.7541532E-48.7133335
CGTCAGA1752.6080306E-78.69022
ATTAGAC3350.08.5120533
GTCTTAG4250.08.4991911
CTAGGAC4050.08.4490013
TAGACTG5350.08.3505865
TTAGACT3950.08.1820064
CGTAGAC1304.164221E-48.0427993
GTATCAA60300.08.0238611
CTACACT5700.08.0046864
CCTATCC3105.456968E-127.9720413
GTCCTAG4800.07.92139531
GACTGCG1200.00169419637.91554647
Produced by FastQC (version 0.11.5)