FastQCFastQC Report
Wed 25 May 2016
SRR1295258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350491
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT166980.38381874597602894No Hit
GGTATCAACGCAGAGTACTTTTTTT146440.3366056842779355No Hit
TATCAACGCAGAGTACTTTTTTTTT94420.21703297397925891No Hit
GTGGTATCAACGCAGAGTACATGGG66520.15290228160453614No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62830.14442048035497604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48300.012.806821
AGCGTCA4500.012.6673643
CAGCGTC4950.011.5157852
GCAGCGT4650.011.44306851
GTGGTAT16500.011.1142661
AGTACCG1201.27573E-711.0843275
ACCGATC600.005878644511.0833068
TGGGTCG700.001491223210.8581145
TGGTATC15650.010.8057062
TGGACCG909.5145515E-510.55655
CTACGAC856.5946847E-410.0593783
GGACCGA856.598867E-410.0586846
TGATCGG800.00451829669.49997710
GTGCGCA800.00451829669.4999779
CTGTCGC2850.08.9999789
ACGACTG850.00743378148.94197755
GACTGCG850.0074386898.9412587
ACCGCCC850.00743939038.9411558
CCGGTCG850.00743939038.9411559
TCGTCAC850.00744368.94053815
Produced by FastQC (version 0.11.5)