FastQCFastQC Report
Wed 25 May 2016
SRR1295257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913447
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA293613.2143080003547No Hit
GCTTATGTACTCTGCGTTGATACCA172291.8861521248632926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA136691.4964196061731003No Hit
CTTATGTACTCTGCGTTGATACCAC121361.3285937771978011No Hit
GAGTACATAAGCAGTGGTATCAACG82530.9035006957163361No Hit
TATGTACTCTGCGTTGATACCACTG70540.7722396592248921No Hit
GCGTTGATACCACTGCTTATGTACT62790.6873962036111564No Hit
GTATCAACGCAGAGTACTTTTTTTT59760.6542251493518507No Hit
CATAAGCAGTGGTATCAACGCAGAG54170.5930283858833627No Hit
CTGCTTATGTACTCTGCGTTGATAC47880.5241683425529888No Hit
ACGCAGAGTACATAAGCAGTGGTAT46860.5130018490399553No Hit
GTATCAACGCAGAGTACATAAGCAG41500.4543230203832297No Hit
GGTATCAACGCAGAGTACTTTTTTT41190.4509292821586803No Hit
ACTCTGCGTTGATACCACTGCTTAT39730.4349458698753184No Hit
TATCAACGCAGAGTACATAAGCAGT36990.40494960298736543No Hit
TATCAACGCAGAGTACTTTTTTTTT35410.38765248558482324No Hit
GGTATCAACGCAGAGTACATAAGCA34840.38141238626871615No Hit
GTGGTATCAACGCAGAGTACATGGG33830.370355368182281No Hit
GTACTGGTTCACTATCGGTCAGTCA31230.3418917572667051No Hit
CCCATGTACTCTGCGTTGATACCAC30740.33652746136338507No Hit
ACATAAGCAGTGGTATCAACGCAGA26280.28770142110051267No Hit
GTGGTATCAACGCAGAGTACATAAG25940.28397925659616813No Hit
AAGCAGTGGTATCAACGCAGAGTAC24510.26832427059260144No Hit
ATACAGGGTGACAGCCCCGTACACA24410.26722951632661773No Hit
GGATACCACGTGTCCCGCCCTACTC21410.23438688834710716No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20280.2220161651414915No Hit
CCACTGCTTATGTACTCTGCGTTGA19730.21599501667858126No Hit
CATGTACTCTGCGTTGATACCACTG19210.21030229449546609No Hit
GATACCACTGCTTATGTACTCTGCG18600.2036242934729656No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16720.1830429132724723No Hit
ATACCACTGCTTATGTACTCTGCGT16240.1777880927957506No Hit
GTTGATACCACTGCTTATGTACTCT15840.17340907573181588No Hit
GTACTCTGCGTTGATACCACTGCTT14320.15676881088886382No Hit
ACCCTGTATCGCGCGCCTTTCCAGA13490.14768235048119924No Hit
TACCACTGCTTATGTACTCTGCGTT13390.14658759621521555No Hit
TGATACCACTGCTTATGTACTCTGC13370.14636864536201882No Hit
CAGTGGTATCAACGCAGAGTACATA13220.14472651396304329No Hit
GGTATCAACGCAGAGTACATGGGGG13110.14352228427046124No Hit
CCCATATTCAGACAGGATACCACGT13080.14319385799066614No Hit
GCAGTGGTATCAACGCAGAGTACAT12970.14198962829808406No Hit
GGTTAATGAGGCGAACCGGGGGAAC12190.1334505450234113No Hit
GTATTTAGCCTTGGAGGATGGTCCC12180.13334106959681294No Hit
GCCTTGGAGGATGGTCCCCCCATAT11920.13049470850525535No Hit
TCTAAGTACCCCGAGGAAAAGAAAT11670.12775782284029616No Hit
GCAGAGTACATAAGCAGTGGTATCA11490.12578726516152552No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10370.11352601738250823No Hit
GTTCACTATCGGTCAGTCAGGAGTA10360.11341654195590987No Hit
GGTATCAACGCAGAGTACATGGGGA10350.1133070665293115No Hit
TATTCAGACAGGATACCACGTGTCC10330.11308811567611476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN10110.11067965629095065No Hit
TCACGGTACTGGTTCACTATCGGTC9970.10914700031857348No Hit
GGTATCAACGCAGAGTACATGGGCA9930.10870909861218No Hit
CTATCGGTCAGTCAGGAGTATTTAG9850.10783329519939307No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG9800.10728591806640123No Hit
GTATCAACGCAGAGTACATGGGGGT9610.10520588496103223No Hit
CCCCATGTACTCTGCGTTGATACCA9270.10148372045668769No Hit
GTCAGGAGTATTTAGCCTTGGAGGA9250.10126476960349096No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9230.10104581875029421No Hit
GTTAATGATAGTGTGTCGAAACACA9170.100388966190704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC250.00603513818.99730512
TCCTTAG350.00216177316.2941553
TCAGTAC350.00216177316.2941553
GACCAAA508.657008E-515.2103881
AGTTCAC508.726328E-515.1961746
TTCAAAC456.7292026E-414.786252
TATTCTA456.7320105E-414.7854375
TTAGGAC400.005258764614.2573863
TGTACCG400.005258764614.2573865
GAGGACG400.00528569514.24641318
CCCATCC400.00528569514.24641316
GTACTAG551.9474252E-413.8276241
AGTTCCT500.001492872613.3068925
GTAAGAC500.001492872613.3068923
TCAAACC500.001492872613.3068923
CGTACTT500.001500937613.29738214
GACTGCT500.001501559613.2966527
CCTTAGG655.4128246E-513.1628361
AGGACCG655.421215E-513.1606635
AGCTATG655.460521E-513.1505367
Produced by FastQC (version 0.11.5)