##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295257_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	913447
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.20071224712545	31.0	29.0	33.0	22.0	34.0
2	29.038220060934023	31.0	28.0	33.0	16.0	34.0
3	29.223108729898943	31.0	29.0	34.0	20.0	34.0
4	32.261520372829516	35.0	32.0	37.0	18.0	37.0
5	32.78298576709979	35.0	33.0	37.0	23.0	37.0
6	32.956020436872635	36.0	33.0	37.0	23.0	37.0
7	32.898155010635534	35.0	33.0	37.0	23.0	37.0
8	32.92334092727876	36.0	33.0	37.0	23.0	37.0
9	33.71798144829421	38.0	33.0	39.0	17.0	39.0
10	33.7704114196007	38.0	33.0	39.0	17.0	39.0
11	34.214396675450246	38.0	34.0	39.0	22.0	39.0
12	34.22919008984648	38.0	34.0	39.0	22.0	39.0
13	34.03308894768936	38.0	33.0	39.0	19.0	39.0
14	34.55858851142978	39.0	33.0	41.0	17.0	41.0
15	34.870597856252196	39.0	33.0	41.0	18.0	41.0
16	34.75573295440239	39.0	33.0	41.0	18.0	41.0
17	34.59022909922524	39.0	33.0	40.0	17.0	41.0
18	34.494750105917475	38.0	33.0	40.0	17.0	41.0
19	34.93520587401349	39.0	34.0	41.0	19.0	41.0
20	35.007747575940364	39.0	34.0	41.0	20.0	41.0
21	35.05617184138762	39.0	34.0	41.0	21.0	41.0
22	34.974441866906346	39.0	33.0	41.0	21.0	41.0
23	34.69202701415627	39.0	33.0	41.0	19.0	41.0
24	34.98278717867594	39.0	33.0	41.0	22.0	41.0
25	33.804449519238666	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.005891634503406351
1101	2	0.08127903533713621
1101	3	-0.1694736347131922
1101	4	0.10605355621622437
1101	5	-0.29492763299255387
1101	6	-0.3526997076722935
1101	7	-0.25129331806017774
1101	8	-0.09857976901329835
1101	9	-0.12128004959043892
1101	10	0.11563166557095172
1101	11	-0.07717353034986729
1101	12	-0.19430885272463172
1101	13	0.14057377888057943
1101	14	0.456396438067344
1101	15	0.1557517948035425
1101	16	-0.10140829280768315
1101	17	0.1278319392124132
1101	18	0.382318042023968
1101	19	0.0289573636556284
1101	20	0.0361518465288313
1101	21	0.20925427774183447
1101	22	0.08158243651821806
1101	23	0.08924600858686205
1101	24	-0.042536419581502116
1101	25	0.10929091427952642
1102	1	0.0234120275544889
1102	2	0.04290812863975191
1102	3	-0.14621168685357944
1102	4	-0.023803751195799805
1102	5	-0.40841737182044824
1102	6	-0.29044060426130613
1102	7	-0.24167202936039445
1102	8	-0.08142609826410308
1102	9	0.015642304014491515
1102	10	0.3201851341527231
1102	11	0.27938304126676883
1102	12	0.08832590596739465
1102	13	0.006434283358821347
1102	14	0.22289024375153588
1102	15	0.22201569381386577
1102	16	-0.017615537923930447
1102	17	-0.09357524008188278
1102	18	0.3183546202227987
1102	19	-0.06813941812109192
1102	20	0.07747063634523954
1102	21	-0.10641275721432208
1102	22	-0.09093454789608302
1102	23	-0.11965358942907756
1102	24	-0.198459689992184
1102	25	-0.10124059797981033
1103	1	-0.06673530988618026
1103	2	-0.05732344372328413
1103	3	-0.12646577002760395
1103	4	0.1593057256845114
1103	5	-0.11573547676760398
1103	6	0.0219330219660705
1103	7	0.005814754569094305
1103	8	0.035733261736808686
1103	9	0.404714769242986
1103	10	0.6725606045934995
1103	11	0.4824765666517905
1103	12	0.21437599062058865
1103	13	0.17875130934607597
1103	14	0.30468491055255953
1103	15	0.34682996536383115
1103	16	0.2831694885842637
1103	17	0.42256875686090467
1103	18	0.9542588884360654
1103	19	0.38675292829823604
1103	20	0.40577627003692385
1103	21	0.4661327991344777
1103	22	0.500771339992852
1103	23	-1.898411197997632
1103	24	-0.7179151907388999
1103	25	-0.07043748106904246
1104	1	-0.32398109775723327
1104	2	-0.03975962208959771
1104	3	-0.28099643436490496
1104	4	-0.5348892031201267
1104	5	-0.3919617102119872
1104	6	-0.28629338126095405
1104	7	-0.35111225674026514
1104	8	-0.5744776208483628
1104	9	-2.2860835419985044
1104	10	-0.9119807820965988
1104	11	-0.6794521834826597
1104	12	-0.5560855914739022
1104	13	-0.2808787670705897
1104	14	-0.08278127159045567
1104	15	-0.23251858055876085
1104	16	-0.47502963848212687
1104	17	-0.013479860785203357
1104	18	0.12576154683676322
1104	19	-0.5173444767766284
1104	20	-0.5113837872454212
1104	21	-0.10265497690099323
1104	22	-0.36386896438112615
1104	23	-0.13839124853691231
1104	24	-0.2829653369111398
1104	25	-0.3182284979272012
1105	1	-0.30823265928190224
1105	2	-0.3632407386741612
1105	3	-0.23743973640810623
1105	4	-0.2527035059306719
1105	5	-0.27272424316204535
1105	6	-0.14199349298302621
1105	7	-0.27084134065904664
1105	8	-0.13403174641442916
1105	9	-2.1687659252966505
1105	10	-0.73676946437255
1105	11	-0.5753091235702072
1105	12	-0.17947834425005738
1105	13	-0.3269685940007747
1105	14	-0.25849056758237055
1105	15	-0.3412821035015412
1105	16	-0.5426229820732189
1105	17	-1.649258456267816
1105	18	-0.33121303142236513
1105	19	-0.4008448962313764
1105	20	-0.3790741421717314
1105	21	-0.34020899909432245
1105	22	-0.46771134879251974
1105	23	-0.14572574282556872
1105	24	-0.26151947042895785
1105	25	-0.7877429840255559
1106	1	-0.2503595381778112
1106	2	-0.4276933832006087
1106	3	-0.29595809277811114
1106	4	-0.35688971728431085
1106	5	-0.3157319359508364
1106	6	-0.22065642791746143
1106	7	-0.38715672415186475
1106	8	-0.41133228636897456
1106	9	-3.0344178318157162
1106	10	-0.9451100943890225
1106	11	-0.379408817109514
1106	12	-0.3131030237171899
1106	13	-0.2238117361610108
1106	14	-0.7735691632835824
1106	15	-0.5059656359300888
1106	16	-0.4167275748140611
1106	17	-0.39271593745279887
1106	18	-0.25460984589898317
1106	19	-0.17866037353869046
1106	20	-0.4294457518200332
1106	21	-0.4428540467312416
1106	22	-0.4051521935399478
1106	23	-0.16532150657981504
1106	24	-0.19702513057030302
1106	25	-0.3960064776774459
1107	1	-0.6310708712068269
1107	2	-0.6048901282157431
1107	3	-0.33788425633829533
1107	4	-2.03702446610934
1107	5	-0.7337511486239592
1107	6	-0.7094509053577411
1107	7	-0.40975137857110866
1107	8	-0.33445762266725865
1107	9	-0.24809672465163857
1107	10	0.058084197401861104
1107	11	-0.0511357900806928
1107	12	-0.7295717578585723
1107	13	-0.5009419228049623
1107	14	-0.4161726411497355
1107	15	-0.7595071278066499
1107	16	-1.2405422110253852
1107	17	-0.29906040380001286
1107	18	-0.454891380158692
1107	19	-0.22170502375022494
1107	20	-0.7596369860060435
1107	21	-0.27545540799019363
1107	22	-0.3523884910584201
1107	23	0.0063256473485324705
1107	24	-0.5804844827864954
1107	25	-0.6529008793788904
1108	1	0.2751891914239266
1108	2	0.24257215869186766
1108	3	0.3225750421445177
1108	4	0.5430368841615376
1108	5	0.5410775395008685
1108	6	0.5697766235520163
1108	7	0.3634395134181432
1108	8	0.31332158414792843
1108	9	0.7643630483636557
1108	10	0.8253947468991711
1108	11	0.4832277997198702
1108	12	0.4256629756594279
1108	13	0.538517712746561
1108	14	0.4242766394251234
1108	15	0.5003737871371712
1108	16	0.6352526779584906
1108	17	0.5234870447405129
1108	18	0.6574471234445554
1108	19	0.5055056479580387
1108	20	0.3495873981844966
1108	21	0.7280936995309588
1108	22	0.5388447096443443
1108	23	0.7556312669806147
1108	24	0.6001047848705383
1108	25	0.8150314583149978
1109	1	0.2596095131256071
1109	2	0.1999313043365909
1109	3	0.3247413279257714
1109	4	0.4954170517511187
1109	5	0.5928102018898969
1109	6	0.5083277207949948
1109	7	0.4011105456176054
1109	8	0.25254106204192794
1109	9	0.7788147198646413
1109	10	0.501372963183492
1109	11	0.15317410952620492
1109	12	0.20809683344267427
1109	13	0.3279430152574747
1109	14	0.5849461920312606
1109	15	0.3232089662998092
1109	16	0.3749321482347838
1109	17	0.8066259067720978
1109	18	0.44149974422917637
1109	19	0.06443667839957357
1109	20	0.30884730852992703
1109	21	0.46659572115353143
1109	22	0.3742674192045641
1109	23	0.5941138780966142
1109	24	0.8123824796348273
1109	25	0.6447793414710716
1110	1	0.20450734116827363
1110	2	0.308738012985863
1110	3	0.4165488291038564
1110	4	0.4599487100641042
1110	5	0.35818151234699513
1110	6	0.43044837795131485
1110	7	0.16729312711955657
1110	8	0.285141841718918
1110	9	0.912409373508126
1110	10	1.0092098950260109
1110	11	0.5483487871597532
1110	12	0.6260593918113457
1110	13	0.25529275607912894
1110	14	0.8008005166681968
1110	15	0.4382354196262881
1110	16	0.40087140197251614
1110	17	0.9356983267695256
1110	18	0.9048399318657303
1110	19	0.3210500100193272
1110	20	0.5450851528515699
1110	21	0.630678636802763
1110	22	0.6100313527178258
1110	23	0.7285117703983701
1110	24	0.8058494718808973
1110	25	0.6938250939450441
1111	1	0.30656316130698613
1111	2	0.1491970722711109
1111	3	0.1182107476891261
1111	4	0.30643071510590403
1111	5	0.33971625082411094
1111	6	0.14499318380254067
1111	7	-0.016128770995806008
1111	8	0.03969035954268918
1111	9	0.5181969874125301
1111	10	0.38350646721757187
1111	11	0.20443813397871935
1111	12	0.11370807343041633
1111	13	-0.08047858641160133
1111	14	0.040506550065138924
1111	15	-0.13326690215848913
1111	16	-0.07045090716271574
1111	17	0.2375258425769431
1111	18	0.37949670954920833
1111	19	0.22905165337805045
1111	20	-0.6178389084788662
1111	21	-0.49546986732635645
1111	22	-0.41432170992982265
1111	23	-0.0018323438727207986
1111	24	-0.0107088087910725
1111	25	0.0332129067823459
1112	1	0.08776424067429289
1112	2	0.06552003004684437
1112	3	0.05147367577416517
1112	4	0.33196363248178784
1112	5	0.17305587836865755
1112	6	0.2149762703578304
1112	7	0.1367077152877627
1112	8	0.2085469621252969
1112	9	-1.0172583043556287
1112	10	-0.10359534524550185
1112	11	-0.02432407341913745
1112	12	-0.05486304171498091
1112	13	-0.17403169561706733
1112	14	-0.21905907742726072
1112	15	-0.03347218336136848
1112	16	-0.12689559498333125
1112	17	0.06989975828666672
1112	18	0.3514326200922824
1112	19	0.2351035374602617
1112	20	0.00460822689073126
1112	21	0.27085484383678704
1112	22	0.046945063019094846
1112	23	0.1094466128707623
1112	24	0.15622266433309306
1112	25	0.42985116857297356
1113	1	0.3664329989159185
1113	2	0.3297950963495033
1113	3	0.5646797671501851
1113	4	0.5864972825086099
1113	5	0.5773777497099175
1113	6	0.401595567421964
1113	7	0.45761123265549486
1113	8	0.38266385643220957
1113	9	0.2882151429927333
1113	10	0.521047601670702
1113	11	0.5486666502577364
1113	12	0.13086505085199462
1113	13	0.17392384362886304
1113	14	-0.019542182851509438
1113	15	0.09628880446421562
1113	16	0.36544448220062975
1113	17	0.6039947152521066
1113	18	0.45779780961773753
1113	19	0.41789858596571605
1113	20	0.45124954194832156
1113	21	0.32423301967694584
1113	22	0.4100819953130994
1113	23	0.02771657390240989
1113	24	0.11726372022895504
1113	25	0.11913265342989376
1114	1	0.2568417451405409
1114	2	0.41491089883144383
1114	3	0.5004358025201974
1114	4	0.8382774766487344
1114	5	0.7574842565071833
1114	6	0.5102931978641294
1114	7	0.390726893176911
1114	8	0.3705239561503788
1114	9	0.3467679463946922
1114	10	0.5750381030552703
1114	11	0.4981619168750768
1114	12	0.5693611064422797
1114	13	0.4811814846998672
1114	14	0.9854937759326177
1114	15	0.5650042527728374
1114	16	0.3205681198117958
1114	17	0.8993144767948209
1114	18	0.5698988527546263
1114	19	0.5845907327274205
1114	20	0.455940609446003
1114	21	0.4349590476850338
1114	22	0.30270614480117075
1114	23	0.889208597267654
1114	24	0.6310484516460022
1114	25	0.6237786757595529
1115	1	0.07200138400574829
1115	2	-0.023118539081494305
1115	3	0.20261975247080954
1115	4	0.3685870690517774
1115	5	0.44704804215444227
1115	6	0.1620312115862248
1115	7	0.1657230327516146
1115	8	0.06137972792020463
1115	9	0.28685186153294495
1115	10	0.16430438468448472
1115	11	0.1904375941855534
1115	12	0.1512674529814646
1115	13	0.024034489755422328
1115	14	-0.0249636364951229
1115	15	0.07630364908455789
1115	16	0.17371891213553425
1115	17	0.29169998206281633
1115	18	0.5633653684763829
1115	19	0.4653986169493791
1115	20	0.42382004356704783
1115	21	0.23469434586062476
1115	22	-0.2517002679819953
1115	23	0.03866964534799422
1115	24	0.34634871050008087
1115	25	0.38269351706181
1116	1	0.4333701185913732
1116	2	0.2615057116300967
1116	3	0.5545377793205688
1116	4	0.7494452203044162
1116	5	0.8113196023130271
1116	6	0.5564299149540801
1116	7	0.7652515301538045
1116	8	0.4955779876019193
1116	9	0.8013997271712299
1116	10	0.5967744441318246
1116	11	0.22209464214414254
1116	12	0.3330286749223106
1116	13	0.2577642763536758
1116	14	0.8709243485886518
1116	15	0.47762754685772535
1116	16	0.6832267675285522
1116	17	1.0874294684637391
1116	18	0.9757153214500534
1116	19	0.5639432107121607
1116	20	0.5580784804202636
1116	21	0.78805992975132
1116	22	0.5243483915267504
1116	23	0.9438761411775971
1116	24	0.6345050995392967
1116	25	0.6086525993392868
1201	1	-0.02393887397116856
1201	2	0.13483835737103433
1201	3	0.02984839918511284
1201	4	0.12503660706367725
1201	5	0.11863168904134369
1201	6	0.1361138516497391
1201	7	-0.06214077568729692
1201	8	0.0275219259019579
1201	9	0.5790589334604093
1201	10	0.4912248859099364
1201	11	0.43469087642979076
1201	12	0.18603013032621618
1201	13	0.16362462633820485
1201	14	0.6143625397622614
1201	15	0.1632094219221898
1201	16	0.3135069614296029
1201	17	0.47969634599207467
1201	18	0.45960617761718936
1201	19	0.057431939926814835
1201	20	0.25513489737629413
1201	21	0.15569495570793634
1201	22	0.16158243651821635
1201	23	-0.2334658558199152
1201	24	0.12424324143545107
1201	25	0.12759599902528862
1202	1	-0.3064302234521392
1202	2	-0.32471709392214265
1202	3	-0.2702325344844674
1202	4	-0.21389248800405625
1202	5	-0.39184628878986416
1202	6	-0.46067226060173994
1202	7	-0.3648045490730212
1202	8	-0.29629251313387783
1202	9	0.44666083364221976
1202	10	0.39135742945576624
1202	11	-0.03920273684801856
1202	12	0.06832676981949959
1202	13	0.20705433720560507
1202	14	0.18743391387521768
1202	15	0.11602391960560965
1202	16	0.10131530111642917
1202	17	0.5538972726239422
1202	18	0.17902439355759725
1202	19	0.15917846505730893
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2211	7	0.2730007701844741
2211	8	0.17838210675350297
2211	9	-1.4825505544298068
2211	10	-0.025391645193806767
2211	11	-0.05681500806150552
2211	12	-0.2306950025860388
2211	13	0.06425351714567995
2211	14	0.3775551602920899
2211	15	0.0801690256846257
2211	16	0.07370553461086615
2211	17	0.33312025656598365
2211	18	0.3573159972200415
2211	19	0.10404937983230411
2211	20	0.4019027439705596
2211	21	0.07385054256666024
2211	22	-0.024740551621924567
2211	23	-1.8522812327454403
2211	24	-0.6809655557654395
2211	25	-0.29663220109285504
2212	1	0.2697524011966159
2212	2	-0.9869543852066016
2212	3	0.05824334856515989
2212	4	0.738711956853237
2212	5	0.546027889701115
2212	6	0.6600587953421524
2212	7	0.47858041644101945
2212	8	0.4321903367767135
2212	9	1.0707754617279548
2212	10	0.923878736438887
2212	11	0.5943473432708828
2212	12	0.4605540752699042
2212	13	0.2751159242565322
2212	14	0.6700376620464112
2212	15	0.6726668215354294
2212	16	0.7076737228901919
2212	17	0.9309646886152123
2212	18	0.9953245259279555
2212	19	0.6094374000564926
2212	20	0.7319475466767145
2212	21	0.5283488062368846
2212	22	0.7335560458914401
2212	23	0.8984690776347506
2212	24	0.5234310471458343
2212	25	0.5657236295565156
2213	1	0.241247314248195
2213	2	0.14951033331861652
2213	3	0.03967087488597443
2213	4	0.4508661057038452
2213	5	0.3334202549617302
2213	6	0.42441698557902896
2213	7	0.489894843926443
2213	8	0.16911126755877604
2213	9	0.6423094527218538
2213	10	0.627686563841614
2213	11	0.19216215926375924
2213	12	0.31751653772554533
2213	13	0.21685162104805045
2213	14	-1.3445220522302108
2213	15	-0.2375947734807724
2213	16	0.06564494153127498
2213	17	0.4451118125240967
2213	18	0.22884294936018534
2213	19	0.07643335139991336
2213	20	0.23991944690981626
2213	21	0.2630977304574813
2213	22	0.38552652841314483
2213	23	0.7056975881744378
2213	24	0.5621840972701122
2213	25	0.37217376201256513
2214	1	-0.11696274665021278
2214	2	0.08547721004378772
2214	3	-0.04649202252059226
2214	4	0.18579774539933425
2214	5	0.2001998228362467
2214	6	0.09439735657264237
2214	7	-0.047628794389808604
2214	8	-0.11896146213022973
2214	9	0.007737169309891101
2214	10	-0.13434585544689526
2214	11	-0.2967720557286384
2214	12	-0.3903030981051998
2214	13	-0.4917676319003377
2214	14	-0.2911394159091856
2214	15	-0.5355960449662689
2214	16	-0.3280854496660197
2214	17	-0.0921487288629379
2214	18	0.1404316058417976
2214	19	-0.04198855191331319
2214	20	0.03245045732774088
2214	21	-0.021387721988418207
2214	22	0.014453452118630139
2214	23	-0.04786724052522118
2214	24	0.029639545890780994
2214	25	0.06392383250098987
2215	1	0.5989296519252463
2215	2	0.4315799784573109
2215	3	0.5393907945753043
2215	4	0.8815954564120503
2215	5	0.767212056835703
2215	6	0.6794523620150628
2215	7	0.6148362878101281
2215	8	0.5866026650920872
2215	9	0.9715063190592588
2215	10	0.7927424315465998
2215	11	0.7913766756059673
2215	12	0.7050766560875772
2215	13	0.25330072420662475
2215	14	0.7078390292843935
2215	15	0.6540388724815287
2215	16	0.7494769796617646
2215	17	0.531979867274174
2215	18	1.029673929209686
2215	19	0.6743036620777616
2215	20	-0.09568509947246895
2215	21	0.47994822511617485
2215	22	-0.4391054334707576
2215	23	0.6063338155510962
2215	24	0.4818122872859476
2215	25	0.594887510943714
2216	1	0.24117557992026306
2216	2	-0.09857974020021487
2216	3	0.4573896081285902
2216	4	0.711534040857785
2216	5	0.6716422975037304
2216	6	0.6679913388741383
2216	7	0.6315338316222352
2216	8	0.46142732546520193
2216	9	0.6296589711498299
2216	10	0.48661746552883045
2216	11	0.47733135264666515
2216	12	-0.035980045816387474
2216	13	0.21832660281557992
2216	14	0.23348703677481808
2216	15	0.48389581271710114
2216	16	0.5192038276654287
2216	17	0.7575272091905774
2216	18	0.9876592975249565
2216	19	0.7184896823307341
2216	20	-0.5033850170465612
2216	21	-0.6166299270668958
2216	22	-0.7012910489768984
2216	23	-0.985657495420412
2216	24	-0.9695151694013688
2216	25	-0.99763839589329
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1842.0
3	232.0
4	191.0
5	192.0
6	177.0
7	261.0
8	226.0
9	518.0
10	1091.0
11	2044.0
12	3567.0
13	5726.0
14	7963.0
15	10909.0
16	12481.0
17	13253.0
18	12537.0
19	11749.0
20	10757.0
21	9841.0
22	9435.0
23	8728.0
24	8620.0
25	8846.0
26	8869.0
27	9642.0
28	10681.0
29	12550.0
30	15324.0
31	19463.0
32	25162.0
33	33475.0
34	46269.0
35	65843.0
36	100099.0
37	164093.0
38	260791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.348367575152935	19.70026531014976	12.661543184160326	21.28982393053698
2	14.400710555236557	25.329230147655394	36.73566181435317	23.534397482754873
3	20.646076981722388	30.68118651776239	28.69259821552158	19.98013828499364
4	13.138554587571317	21.705031955201182	31.665868152927835	33.490545304299665
5	11.629977152163951	43.709372008708335	30.354872281805363	14.305778557322352
6	28.576057823278706	35.656773144690014	20.689397600949075	15.07777143108221
7	22.93523359034551	38.79239794668544	18.925492100143572	19.346876362825473
8	22.648729834523053	37.241267165994444	22.239899270125925	17.870103729356586
9	26.921733284117277	22.523377627785983	17.241220206707446	33.3136688813893
10	15.783389376057668	30.418342454012	27.24357706424035	26.554691105689987
11	28.959856298680908	30.484267296750687	19.181512948270598	21.3743634562978
12	24.140518155204894	30.016532193838604	23.455393284963392	22.387556365993113
13	27.537339575172766	25.132737920711946	27.733248117509984	19.596674386605304
14	26.156826738480056	28.28464988775497	22.513805480017748	23.044717893747226
15	28.820583588416127	27.67122775945381	22.80714861749886	20.701040034631195
16	23.61533739569115	28.845961672982757	25.524001330936475	22.014699600389616
17	20.123229455531483	32.87455557850604	24.623697498934266	22.37851746702821
18	19.66663042129207	27.59349384542556	32.68860500339938	20.051270729882994
19	22.434452485466842	32.677638487936264	23.445622036795367	21.442286989801524
20	22.08129045718333	30.203198691095164	27.643147740437147	20.072363111284364
21	24.440018543378102	31.185392036453997	23.53756681877803	20.837022601389872
22	22.21919655307963	29.756589797590056	23.069946394415005	24.95426725491531
23	25.73617641666548	26.495654245467886	23.58037038541469	24.187798952451946
24	21.254570075218854	29.145685596396874	22.69535870354283	26.904385624841442
25	24.30400820551566	31.201055998910615	23.600631227302227	20.894304568271494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16200.0
1	16200.0
2	11008.0
3	5816.0
4	5816.0
5	5816.0
6	6765.0
7	7714.0
8	7714.0
9	7714.0
10	7604.5
11	7495.0
12	7495.0
13	7495.0
14	8165.0
15	8835.0
16	8835.0
17	8835.0
18	11433.0
19	14031.0
20	14031.0
21	14031.0
22	17696.5
23	21362.0
24	21362.0
25	21362.0
26	26560.5
27	31759.0
28	31759.0
29	31759.0
30	34976.0
31	38193.0
32	38193.0
33	38193.0
34	42862.5
35	47532.0
36	47532.0
37	47532.0
38	55245.0
39	62958.0
40	62958.0
41	62958.0
42	127176.0
43	191394.0
44	191394.0
45	191394.0
46	159182.5
47	126971.0
48	126971.0
49	126971.0
50	115466.0
51	103961.0
52	103961.0
53	103961.0
54	91765.0
55	79569.0
56	79569.0
57	79569.0
58	67958.5
59	56348.0
60	56348.0
61	56348.0
62	51352.5
63	46357.0
64	46357.0
65	46357.0
66	34776.0
67	23195.0
68	23195.0
69	23195.0
70	18775.0
71	14355.0
72	14355.0
73	14355.0
74	9987.0
75	5619.0
76	5619.0
77	5619.0
78	4007.5
79	2396.0
80	2396.0
81	2396.0
82	1647.0
83	898.0
84	898.0
85	898.0
86	632.5
87	367.0
88	367.0
89	367.0
90	229.5
91	92.0
92	92.0
93	92.0
94	58.5
95	25.0
96	25.0
97	25.0
98	15.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14308438256406775
2	0.28573086342174203
3	0.3451760200646562
4	0.3747343852462157
5	0.38469664906666723
6	0.33795064190916385
7	0.3409064784273198
8	0.3601741535086327
9	0.3475844794498203
10	0.3751722869526092
11	0.2921899135910458
12	0.33959277330813936
13	0.3085017521542027
14	0.3217482787726053
15	0.35962677637564083
16	0.30762594874141574
17	0.3585320221096572
18	0.32689362382272863
19	0.3410159538539182
20	0.3023711282646941
21	0.34473811835826274
22	0.29788263577416096
23	0.28003814123862686
24	0.31791663884166244
25	0.3109102115393668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	913447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.36515492901215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95785192311723	18.44862443045634
2	6.762615425757514	3.160191142963035
3	3.037281412060132	2.1289965226738135
4	1.802347199677882	1.6844848622537962
5	1.213126057103756	1.417243913632545
6	0.954551433091151	1.3381945273131188
7	0.7380642635715947	1.20714901061423
8	0.6016301975546848	1.1245746220669917
9	0.5196017119678169	1.0926517051357187
>10	4.532686935062678	21.876908762024456
>50	0.5434996955356511	8.725084155425987
>100	0.28917574788387895	12.448496872116655
>500	0.02401948394475216	3.9877358443103437
>1k	0.019309781210487032	9.763999495827928
>5k	0.0023548513671325646	3.629105922222827
>10k+	0.0018838810937060518	7.966558210962176
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	29361	3.2143080003547	No Hit
GCTTATGTACTCTGCGTTGATACCA	17229	1.8861521248632926	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	13669	1.4964196061731003	No Hit
CTTATGTACTCTGCGTTGATACCAC	12136	1.3285937771978011	No Hit
GAGTACATAAGCAGTGGTATCAACG	8253	0.9035006957163361	No Hit
TATGTACTCTGCGTTGATACCACTG	7054	0.7722396592248921	No Hit
GCGTTGATACCACTGCTTATGTACT	6279	0.6873962036111564	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5976	0.6542251493518507	No Hit
CATAAGCAGTGGTATCAACGCAGAG	5417	0.5930283858833627	No Hit
CTGCTTATGTACTCTGCGTTGATAC	4788	0.5241683425529888	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	4686	0.5130018490399553	No Hit
GTATCAACGCAGAGTACATAAGCAG	4150	0.4543230203832297	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4119	0.4509292821586803	No Hit
ACTCTGCGTTGATACCACTGCTTAT	3973	0.4349458698753184	No Hit
TATCAACGCAGAGTACATAAGCAGT	3699	0.40494960298736543	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3541	0.38765248558482324	No Hit
GGTATCAACGCAGAGTACATAAGCA	3484	0.38141238626871615	No Hit
GTGGTATCAACGCAGAGTACATGGG	3383	0.370355368182281	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3123	0.3418917572667051	No Hit
CCCATGTACTCTGCGTTGATACCAC	3074	0.33652746136338507	No Hit
ACATAAGCAGTGGTATCAACGCAGA	2628	0.28770142110051267	No Hit
GTGGTATCAACGCAGAGTACATAAG	2594	0.28397925659616813	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2451	0.26832427059260144	No Hit
ATACAGGGTGACAGCCCCGTACACA	2441	0.26722951632661773	No Hit
GGATACCACGTGTCCCGCCCTACTC	2141	0.23438688834710716	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2028	0.2220161651414915	No Hit
CCACTGCTTATGTACTCTGCGTTGA	1973	0.21599501667858126	No Hit
CATGTACTCTGCGTTGATACCACTG	1921	0.21030229449546609	No Hit
GATACCACTGCTTATGTACTCTGCG	1860	0.2036242934729656	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1672	0.1830429132724723	No Hit
ATACCACTGCTTATGTACTCTGCGT	1624	0.1777880927957506	No Hit
GTTGATACCACTGCTTATGTACTCT	1584	0.17340907573181588	No Hit
GTACTCTGCGTTGATACCACTGCTT	1432	0.15676881088886382	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1349	0.14768235048119924	No Hit
TACCACTGCTTATGTACTCTGCGTT	1339	0.14658759621521555	No Hit
TGATACCACTGCTTATGTACTCTGC	1337	0.14636864536201882	No Hit
CAGTGGTATCAACGCAGAGTACATA	1322	0.14472651396304329	No Hit
GGTATCAACGCAGAGTACATGGGGG	1311	0.14352228427046124	No Hit
CCCATATTCAGACAGGATACCACGT	1308	0.14319385799066614	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1297	0.14198962829808406	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1219	0.1334505450234113	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1218	0.13334106959681294	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1192	0.13049470850525535	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1167	0.12775782284029616	No Hit
GCAGAGTACATAAGCAGTGGTATCA	1149	0.12578726516152552	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1037	0.11352601738250823	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1036	0.11341654195590987	No Hit
GGTATCAACGCAGAGTACATGGGGA	1035	0.1133070665293115	No Hit
TATTCAGACAGGATACCACGTGTCC	1033	0.11308811567611476	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1011	0.11067965629095065	No Hit
TCACGGTACTGGTTCACTATCGGTC	997	0.10914700031857348	No Hit
GGTATCAACGCAGAGTACATGGGCA	993	0.10870909861218	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	985	0.10783329519939307	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	980	0.10728591806640123	No Hit
GTATCAACGCAGAGTACATGGGGGT	961	0.10520588496103223	No Hit
CCCCATGTACTCTGCGTTGATACCA	927	0.10148372045668769	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	925	0.10126476960349096	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	923	0.10104581875029421	No Hit
GTTAATGATAGTGTGTCGAAACACA	917	0.100388966190704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.379017063934744E-4	0.0	0.0	0.0	0.0
6	6.568525595902116E-4	0.0	0.0	0.0	0.0
7	6.568525595902116E-4	0.0	0.0	0.0	0.0
8	7.663279861885801E-4	0.0	0.0	0.0	0.0
9	7.663279861885801E-4	0.0	0.0	1.094754265983686E-4	0.0
10	9.852788393853174E-4	0.0	0.0	1.094754265983686E-4	0.0
11	9.852788393853174E-4	0.0	0.0	2.189508531967372E-4	0.0
12	9.852788393853174E-4	0.0	0.0	2.189508531967372E-4	0.0
13	0.001094754265983686	0.0	0.0	2.189508531967372E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTGC	25	0.006035138	18.997305	12
TCCTTAG	35	0.002161773	16.294155	3
TCAGTAC	35	0.002161773	16.294155	3
GACCAAA	50	8.657008E-5	15.210388	1
AGTTCAC	50	8.726328E-5	15.196174	6
TTCAAAC	45	6.7292026E-4	14.78625	2
TATTCTA	45	6.7320105E-4	14.785437	5
TTAGGAC	40	0.0052587646	14.257386	3
TGTACCG	40	0.0052587646	14.257386	5
GAGGACG	40	0.005285695	14.246413	18
CCCATCC	40	0.005285695	14.246413	16
GTACTAG	55	1.9474252E-4	13.827624	1
AGTTCCT	50	0.0014928726	13.306892	5
GTAAGAC	50	0.0014928726	13.306892	3
TCAAACC	50	0.0014928726	13.306892	3
CGTACTT	50	0.0015009376	13.297382	14
GACTGCT	50	0.0015015596	13.296652	7
CCTTAGG	65	5.4128246E-5	13.162836	1
AGGACCG	65	5.421215E-5	13.160663	5
AGCTATG	65	5.460521E-5	13.150536	7
>>END_MODULE