FastQCFastQC Report
Wed 25 May 2016
SRR1295257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913447
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA317733.4783627293099655No Hit
GCTTATGTACTCTGCGTTGATACCA185832.034381852477484No Hit
GTATCAACGCAGAGTACTTTTTTTT175011.915929440898049No Hit
CTTATGTACTCTGCGTTGATACCAC142561.5606816815863427No Hit
GGTATCAACGCAGAGTACTTTTTTT126011.3794998505660427No Hit
TATCAACGCAGAGTACTTTTTTTTT101861.1151166953309826No Hit
GAGTACATAAGCAGTGGTATCAACG88050.9639311311986355No Hit
CATAAGCAGTGGTATCAACGCAGAG69060.7560372960883336No Hit
GCGTTGATACCACTGCTTATGTACT65300.7148745356873469No Hit
TATGTACTCTGCGTTGATACCACTG61620.6745875786991473No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58370.6390080650546774No Hit
CTGCTTATGTACTCTGCGTTGATAC53680.5876640899800427No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52480.5745270387882384No Hit
ACGCAGAGTACATAAGCAGTGGTAT52410.5737607108020498No Hit
ACTCTGCGTTGATACCACTGCTTAT45710.5004121749811429No Hit
GTATCAACGCAGAGTACATAAGCAG42130.46121997225892686No Hit
GGTATCAACGCAGAGTACATAAGCA39160.42870577055921144No Hit
TATCAACGCAGAGTACATAAGCAGT38830.42509308148146524No Hit
CCCATGTACTCTGCGTTGATACCAC35810.3920315026487579No Hit
GTGGTATCAACGCAGAGTACATGGG35600.3897325186901922No Hit
GTACTGGTTCACTATCGGTCAGTCA33280.3643342197193707No Hit
CTTATACACATCTCCGAGCCCACGA32530.356123562724493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30140.3299589357674829No Hit
ACATAAGCAGTGGTATCAACGCAGA29780.3260178204099417No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28070.3072975224616207No Hit
GTGGTATCAACGCAGAGTACATAAG27380.29974371802633326No Hit
ATACAGGGTGACAGCCCCGTACACA27360.2995247671731365No Hit
AAGCAGTGGTATCAACGCAGAGTAC26280.28770142110051267No Hit
GGATACCACGTGTCCCGCCCTACTC22410.24533443100694402No Hit
CATGTACTCTGCGTTGATACCACTG22380.24500600472714892No Hit
CCACTGCTTATGTACTCTGCGTTGA21920.23997013510362397No Hit
GATACCACTGCTTATGTACTCTGCG21450.23482479005350063No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21400.23427741292050883No Hit
ATACCACTGCTTATGTACTCTGCGT17470.19125357026734993No Hit
GTGGTATCAACGCAGAGTACTTTTT17180.18807878289599725No Hit
ATCAACGCAGAGTACTTTTTTTTTT16650.1822765852862837No Hit
GTTGATACCACTGCTTATGTACTCT16590.1816197327266935No Hit
CCCATATTCAGACAGGATACCACGT16470.18030602760751308No Hit
ACCCTGTATCGCGCGCCTTTCCAGA15920.1742848791446028No Hit
CAGTGGTATCAACGCAGAGTACATA15560.17034376378706154No Hit
ATACACATCTCCGAGCCCACGAGAC15260.1670595009891105No Hit
GGTATCAACGCAGAGTACATGGGGG14620.1600530736868149No Hit
GCAGTGGTATCAACGCAGAGTACAT14600.15983412283361814No Hit
GTACTCTGCGTTGATACCACTGCTT14530.15906779484742958No Hit
GCAGAGTACATAAGCAGTGGTATCA13600.14888658017378129No Hit
TACCACTGCTTATGTACTCTGCGTT12970.14198962829808406No Hit
GCCTTGGAGGATGGTCCCCCCATAT12460.13640638154156726No Hit
GGTTAATGAGGCGAACCGGGGGAAC12250.13410739758300153No Hit
TGATACCACTGCTTATGTACTCTGC11910.130385233078657No Hit
GGTATCAACGCAGAGTACATGGGGA11470.1255683143083288No Hit
TCTAAGTACCCCGAGGAAAAGAAAT11470.1255683143083288No Hit
GGTATCAACGCAGAGTACATGGGCA11200.12261247779017284No Hit
TCACGGTACTGGTTCACTATCGGTC11030.12075139553800057No Hit
GTATTTAGCCTTGGAGGATGGTCCC10980.12020401840500872No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10970.12009454297841034No Hit
TATTCAGACAGGATACCACGTGTCC10640.11648185390066418No Hit
CTATCGGTCAGTCAGGAGTATTTAG10600.1160439521942707No Hit
GTCAGGAGTATTTAGCCTTGGAGGA10540.1153870996346805No Hit
ATCAACGCAGAGTACATAAGCAGTG10530.11527762420808214No Hit
CGGTACTGGTTCACTATCGGTCAGT10440.11429234536869681No Hit
GCCCAGAGCCTGAATCAGTGTGTGT10320.1129786402495164No Hit
GTATCAACGCAGAGTACATGGGGGT10050.11002280373136045No Hit
GTTCACTATCGGTCAGTCAGGAGTA9870.1080522460525898No Hit
CCCCATGTACTCTGCGTTGATACCA9850.10783329519939307No Hit
GTACATGGGAAGCAGTGGTATCAAC9530.10433008154824526No Hit
GTTAATGATAGTGTGTCGAAACACA9530.10433008154824526No Hit
CACACACACTGATTCAGGCTCTGGG9470.10367322898865505No Hit
CATCTAAGTACCCCGAGGAAAAGAA9370.10257847472267137No Hit
CCCCAGTAGCGGCGAGCGAACGGGG9330.1021405730162779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGAC250.006018982719.0061341
CCCCAAT250.006018982719.0061341
GGCAGGT250.00603232318.9988515
TAAGACC504.5488996E-617.1036473
GACGCAT453.5381454E-516.88786713
CGCAAAA350.0021661916.2891882
AAATCGC702.5007466E-816.28472917
AATCGCC702.5007466E-816.28472918
AGCTGAG350.00217094916.2838386
GTAAGAC601.4619418E-615.836713
TATACAC4750.015.80336953
CTTATAC4400.015.3344951
GATTTGG508.683728E-515.2049071
CGCATGA508.712167E-515.1990815
CCTTATA902.2755557E-914.7809292
CTTGCAC456.7476335E-414.7809293
GTGCAAG456.761655E-414.77688312
CACCGGT456.7644625E-414.7760747
GAGACCG1101.2732926E-1114.68092919
TTATACA5300.014.3426812
Produced by FastQC (version 0.11.5)