FastQCFastQC Report
Wed 25 May 2016
SRR1295256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553478
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG73350.1320793924095855No Hit
GTATCAACGCAGAGTACTTTTTTTT69070.12437251034396823No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN63410.11418069901420334No Hit
GGTATCAACGCAGAGTACTTTTTTT57160.10292649039034638No Hit
ATTCCATTCCATTCCATTCCATTCC55570.10006341971643716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG801.9928866E-613.0678333
CCGCGCA550.003072406412.08972559
GGTATCA40050.011.1061571
GCGTCAG1752.0008883E-1110.8620661
TACGACC750.00264005310.1376534
TAGACAG7450.09.9503695
GTGGTAT17900.09.6635981
TGGTATC17000.09.3918982
CGTCAGA1901.0528311E-89.0035112
ACGGATC950.0018263228.9992818
TGCCGAC850.00744741228.94005912
GTCTTAC5100.08.5725131
TAGACTG6900.08.5397175
TCTAGAC6350.08.3813733
GTACCGT1252.748506E-48.359186
CTTACAC8000.08.3158943
GCGTAGC1150.00110708758.2640922
GGGTACG1050.0044972218.1465491
TATACTG10150.08.1461815
TAATACG1659.699752E-68.0640434
Produced by FastQC (version 0.11.5)