FastQCFastQC Report
Wed 25 May 2016
SRR1295256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553478
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT187610.3378243327874892No Hit
GGTATCAACGCAGAGTACTTTTTTT159870.2878736532313624No Hit
TATCAACGCAGAGTACTTTTTTTTT104710.18854850960065025No Hit
GTGGTATCAACGCAGAGTACATGGG75470.13589681997479777No Hit
ACGCAGAGTACTTTTTTTTTTTTTT72340.1302607122959702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA4850.012.73243
GGTATCA56250.012.3315371
CAGCGTC5400.011.6115132
GCAGCGT5750.010.5761841
TATATCG1002.4000303E-510.450555
GTGGTAT20950.010.4318341
GAACCGT1201.5269707E-610.2910966
GACCGTC951.645876E-49.9998057
TGGTATC20150.09.9953832
TACACCG1401.059343E-69.50055
GCGTCAG6700.09.2167374
CTTACAC7950.08.9625743
TAGTACG1304.371377E-58.7695344
ACGGCCA1304.371839E-58.76945513
ACCGTCC1107.1490876E-48.6364298
CCCTTAC6250.08.5138281
TAGGACT6750.08.44473654
ACACCGT1804.0434497E-78.4439766
GTCTTAG5100.08.3841521
CGAATAG2752.3646862E-118.29097210
Produced by FastQC (version 0.11.5)