FastQCFastQC Report
Wed 25 May 2016
SRR1295255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA666463.0456147598481165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA470852.1517085941759233No Hit
GCTTATGTACTCTGCGTTGATACCA400101.8283924997977845No Hit
CTTATGTACTCTGCGTTGATACCAC273591.2502621945005647No Hit
GAGTACATAAGCAGTGGTATCAACG183100.836737482411833No Hit
TATGTACTCTGCGTTGATACCACTG158660.725050622389194No Hit
GTATCAACGCAGAGTACTTTTTTTT146810.6708980327300993No Hit
GCGTTGATACCACTGCTTATGTACT134850.6162427608041271No Hit
CATAAGCAGTGGTATCAACGCAGAG121770.5564692694335822No Hit
GGTATCAACGCAGAGTACTTTTTTT105020.4799244697044822No Hit
ACTCTGCGTTGATACCACTGCTTAT104230.47631429706054257No Hit
ACGCAGAGTACATAAGCAGTGGTAT104190.4761315035089507No Hit
CTGCTTATGTACTCTGCGTTGATAC102700.4693224437121532No Hit
CCCATGTACTCTGCGTTGATACCAC93110.42549768971799984No Hit
GTGGTATCAACGCAGAGTACATGGG91290.41718058312056927No Hit
TATCAACGCAGAGTACTTTTTTTTT90830.41507845727726267No Hit
GTATCAACGCAGAGTACATAAGCAG87470.39972379894354465No Hit
TATCAACGCAGAGTACATAAGCAGT85170.38921316972701153No Hit
GGTATCAACGCAGAGTACATAAGCA79130.3616113434366376No Hit
GTACATGATAAGCAGTGGTATCAAC76820.3510550158322065No Hit
ATCATGTACTCTGCGTTGATACCAC76380.3490442867646958No Hit
CATGTACTCTGCGTTGATACCACTG73030.3337353268188758No Hit
GTACTGGTTCACTATCGGTCAGTCA64830.29626264874254027No Hit
AAGCAGTGGTATCAACGCAGAGTAC59240.270717249907575No Hit
GTGGTATCAACGCAGAGTACATAAG56260.2570991303139799No Hit
ACATAAGCAGTGGTATCAACGCAGA56100.2563679561076124No Hit
ATACAGGGTGACAGCCCCGTACACA51880.237083236414669No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50000.2284919394898506No Hit
GGATACCACGTGTCCCGCCCTACTC44240.2021696680606198No Hit
CCACTGCTTATGTACTCTGCGTTGA43950.2008444148115787No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41680.19047088075873947No Hit
GATACCACTGCTTATGTACTCTGCG40770.1863123274600242No Hit
GTACTCTGCGTTGATACCACTGCTT38580.17630438051036873No Hit
GCAGTGGTATCAACGCAGAGTACAT38180.17447644499444995No Hit
GGTATCAACGCAGAGTACATGGGGG38020.1737452707880824No Hit
ATACCACTGCTTATGTACTCTGCGT37260.1702721933078367No Hit
GTTGATACCACTGCTTATGTACTCT35710.1631889431836513No Hit
GTATCAACGCAGAGTACATGGGGGT34600.15811642212697663No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA33120.15135306071807705No Hit
GTACATGGGAAGCAGTGGTATCAAC33050.15103317200279126No Hit
GTTAATGATAGTGTGTCGAAACACA32320.14769718968623943No Hit
GAGTACATGATAAGCAGTGGTATCA31000.1416650024837074No Hit
CCCATATTCAGACAGGATACCACGT30590.1397913685798906No Hit
GGTATCAACGCAGAGTACATGGGGA29790.136135497548053No Hit
CAGTGGTATCAACGCAGAGTACATA29530.13494733946270576No Hit
TACCACTGCTTATGTACTCTGCGTT29160.13325649911048087No Hit
ACCCTGTATCGCGCGCCTTTCCAGA28630.13083448455188845No Hit
TGATACCACTGCTTATGTACTCTGC28560.13051459583660266No Hit
GCTTATCATGTACTCTGCGTTGATA27760.12685872480476507No Hit
ATCATTAACTGAATCCATAGGTTAA27460.12548777316782597No Hit
GTATTTAGCCTTGGAGGATGGTCCC25800.11790184077676291No Hit
GCAGAGTACATAAGCAGTGGTATCA25750.11767334883727307No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG25330.11575401654555831No Hit
GTTCACTATCGGTCAGTCAGGAGTA24500.1119610503500268No Hit
GCCTTGGAGGATGGTCCCCCCATAT24300.1110470825920674No Hit
TATTCAGACAGGATACCACGTGTCC23900.10921914707614859No Hit
GGTTAATGAGGCGAACCGGGGGAAC23420.10702562445704604No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN23310.10652294219016835No Hit
GCCCAGAGCCTGAATCAGTGTGTGT23030.10524338732902519No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22600.10327835664941248No Hit
TCTAAGTACCCCGAGGAAAAGAAAT22590.1032326582615145No Hit
ACTTAGATGTTTCAGTTCCCCCGGT22490.10277567438253479No Hit
CTATCGGTCAGTCAGGAGTATTTAG22280.10181600823667744No Hit
GCGTTGATACCACTGCTTATCATGT22110.10103913564241195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCC651.9019171E-716.086114
CTAGTTC704.4108856E-714.9367593
CTAATAG801.2806049E-714.2578173
CCGTCTT1101.8735591E-1013.81529615
ATCGATT551.9645807E-413.81497916
GCCTAGT707.2144467E-613.5807431
GACTGTC500.001501209913.2984427
CACGCAT1153.8016879E-1013.21372117
TAGTCAG655.4651515E-513.15109815
ACATAAT1251.0186341E-1012.9270873
GAACGCA751.4705669E-512.6739054
GATACAC604.074311E-412.6736143
TATTGTA905.372458E-712.6736142
ATAAGAC905.372458E-712.6736143
GTGGGCA1052.0008883E-812.664021513
CGATAGT905.431066E-712.66227818
TCTAGGA1457.2759576E-1212.4551042
CATAATC1001.4327452E-712.3570574
CGAATGG3550.012.30559419
CGCAAGT853.957637E-612.29070517
Produced by FastQC (version 0.11.5)