##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295255_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2188261
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.468004502205176	31.0	30.0	33.0	22.0	34.0
2	29.36210808491309	31.0	30.0	34.0	22.0	34.0
3	29.640730698943134	31.0	30.0	34.0	23.0	34.0
4	32.55299025116291	35.0	33.0	37.0	19.0	37.0
5	33.264930006064176	35.0	33.0	37.0	26.0	37.0
6	33.13128415668881	36.0	33.0	37.0	25.0	37.0
7	33.18903183852383	36.0	33.0	37.0	25.0	37.0
8	33.15849023493998	37.0	33.0	37.0	25.0	37.0
9	33.93917818761108	38.0	33.0	39.0	18.0	39.0
10	34.04362550902292	38.0	33.0	39.0	19.0	39.0
11	34.431895007039834	39.0	34.0	39.0	23.0	39.0
12	34.47132951690863	39.0	34.0	39.0	23.0	39.0
13	34.24854759098663	38.0	34.0	39.0	22.0	39.0
14	34.83022089229758	39.0	33.0	41.0	18.0	41.0
15	35.12698165346821	39.0	34.0	41.0	22.0	41.0
16	35.004956447151415	39.0	33.0	41.0	21.0	41.0
17	34.8805750319546	39.0	33.0	41.0	20.0	41.0
18	34.75507537720592	39.0	33.0	41.0	19.0	41.0
19	35.16386116646963	39.0	34.0	41.0	22.0	41.0
20	35.21927594560247	39.0	34.0	41.0	22.0	41.0
21	35.272347768387775	39.0	34.0	41.0	23.0	41.0
22	35.203709703732784	39.0	34.0	41.0	23.0	41.0
23	34.925411091272935	39.0	33.0	41.0	22.0	41.0
24	35.22536799769315	39.0	33.0	41.0	24.0	41.0
25	34.05633057482631	38.0	31.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.06956213622478913
1101	2	0.09678653558329842
1101	3	-0.11633517108057845
1101	4	0.032286005307398113
1101	5	-0.09595721746819663
1101	6	-0.22239487839102168
1101	7	-0.1378879591706692
1101	8	-0.20064537322511455
1101	9	-0.15588111801079663
1101	10	-0.06553963534750551
1101	11	-0.08402911955798231
1101	12	-0.19184644683196694
1101	13	-0.11805607333228352
1101	14	0.23988795017401543
1101	15	0.04753800641065453
1101	16	-0.1248856090791648
1101	17	-0.08515455074959988
1101	18	0.12084974591402187
1101	19	-0.16551754977199806
1101	20	-0.14107088925231892
1101	21	-0.1746907423329489
1101	22	-0.34602373923011953
1101	23	-0.14242531307308326
1101	24	-0.47779281814430163
1101	25	-0.031703363627642034
1102	1	-0.10466158440726048
1102	2	0.10017845081580035
1102	3	-4.616195342670437E-4
1102	4	0.16331723838478496
1102	5	-0.07931143843075716
1102	6	-0.28649810301214274
1102	7	-0.1333340393639162
1102	8	-0.10085412757551637
1102	9	0.07715673996612793
1102	10	0.30780354276091515
1102	11	-0.07329874150662619
1102	12	0.024322275216029254
1102	13	-0.11997736939570558
1102	14	0.20623666352958736
1102	15	0.08978751719504174
1102	16	-0.03938879801196293
1102	17	-0.14257800198346615
1102	18	0.2034893569275269
1102	19	-0.1652357623925198
1102	20	-0.15654338409087387
1102	21	-0.05159721483560986
1102	22	-0.21361435958203856
1102	23	-0.22121288146342977
1102	24	-0.31857319338591594
1102	25	-0.3288862113223274
1103	1	-0.16709517214417247
1103	2	-0.10055960911785533
1103	3	-0.158289478948344
1103	4	0.02572365517299602
1103	5	-0.3170756228780718
1103	6	-0.37714675133319986
1103	7	-0.3035952845004175
1103	8	-0.12917676087477759
1103	9	0.1850981315227358
1103	10	0.1017863392070808
1103	11	0.025078981143643375
1103	12	-0.1368969024233948
1103	13	-0.07512217338104676
1103	14	-0.04376868985436033
1103	15	-0.08133804693041924
1103	16	-0.05282740782905648
1103	17	-0.04416381571802219
1103	18	0.25616271566081394
1103	19	-0.1454927854262067
1103	20	-0.025009528482755172
1103	21	-0.06378838518634922
1103	22	-0.3024293223911698
1103	23	-2.288193209292544
1103	24	-1.124266971743694
1103	25	-0.4370275972225244
1104	1	-0.2728205920451501
1104	2	-0.06337201781357749
1104	3	-0.3405268977122127
1104	4	-0.611111353552733
1104	5	-0.3877082184070204
1104	6	-0.5661916197365926
1104	7	-0.43270170679406306
1104	8	-0.3379265733308543
1104	9	-2.7867683056164907
1104	10	-0.956504681584633
1104	11	-0.5953433677749231
1104	12	-0.46706008537940136
1104	13	-0.4305848343727021
1104	14	-0.18763402936808404
1104	15	-0.36734430892335723
1104	16	-0.36622988893647346
1104	17	0.02729081444535808
1104	18	0.048752756486166504
1104	19	-0.3483148279572248
1104	20	-0.42187655786595
1104	21	-0.5247524004425514
1104	22	-0.4029680669918818
1104	23	-0.2934041665956997
1104	24	-0.3341278338929925
1104	25	-0.1918405665758769
1105	1	-0.18092216725038668
1105	2	-0.09276480051448033
1105	3	-0.2608578376749442
1105	4	0.020712211603850506
1105	5	-0.08651270082322782
1105	6	-0.14141908875054554
1105	7	-0.22099282940568798
1105	8	-0.21007908434893352
1105	9	-2.221708058159308
1105	10	-0.5345467975416227
1105	11	-0.3813843896709628
1105	12	-0.21536004671000342
1105	13	-0.14837302579024936
1105	14	-0.23531460668733928
1105	15	-0.08979505933868381
1105	16	-0.19300312324741498
1105	17	-1.2126319006582094
1105	18	-0.25569260716110165
1105	19	-0.37672874947855917
1105	20	-0.27330119498668637
1105	21	-0.17473710784188512
1105	22	-0.10890011395026988
1105	23	-0.25627346269143914
1105	24	-0.32658595439868066
1105	25	-0.8845502402573757
1106	1	-0.3445342408084002
1106	2	-0.4088348549811407
1106	3	-0.42433009177115366
1106	4	-0.496173102416698
1106	5	-0.18632314810981399
1106	6	-0.5217298930585628
1106	7	-0.5683849997911423
1106	8	-0.6251618678928708
1106	9	-3.0197146574019236
1106	10	-0.9495799264946925
1106	11	-0.7166034175461675
1106	12	-0.6383581653418062
1106	13	-0.5631269091233975
1106	14	-0.7023638201881184
1106	15	-0.6234266545253533
1106	16	-0.7878642368986135
1106	17	-0.8110039380244203
1106	18	-0.34327032642839583
1106	19	-0.5473033799353288
1106	20	-0.4055078705695436
1106	21	-0.42381613808870355
1106	22	-0.49466476220206346
1106	23	-0.4504847599859616
1106	24	-0.403163067781648
1106	25	-0.7267408710162044
1107	1	-0.3126722647868583
1107	2	-0.10598194857356802
1107	3	-0.12415186742293116
1107	4	-1.476445205885394
1107	5	-0.3614661000648667
1107	6	-0.401912865541199
1107	7	-0.22651160868256426
1107	8	-0.06872961308585701
1107	9	0.21496527243705543
1107	10	0.3346230077169352
1107	11	0.08219306958684314
1107	12	-0.5090461858212407
1107	13	-0.12905825456108744
1107	14	0.1712295167721578
1107	15	-0.5986332660956037
1107	16	-0.8376509847124751
1107	17	-0.02939912126403499
1107	18	-0.2694522619967401
1107	19	-0.08505198916837742
1107	20	-0.37489594816203464
1107	21	-0.08647680148811787
1107	22	-0.20999698487828056
1107	23	0.13734293465557101
1107	24	-0.16467541255489948
1107	25	-0.4052923589280226
1108	1	0.5123687806833885
1108	2	0.5347631630231824
1108	3	0.5615553790311871
1108	4	0.8549402893223359
1108	5	0.6460417688826823
1108	6	0.6243810500734313
1108	7	0.6665442202468697
1108	8	0.5585065336670212
1108	9	0.9691435882071247
1108	10	0.9965290300487766
1108	11	0.7079316432808085
1108	12	0.5778364313925692
1108	13	0.5371810485487813
1108	14	0.5883614723461577
1108	15	0.5743142662638974
1108	16	0.9808229543258449
1108	17	0.9511312473197293
1108	18	0.8959453684228578
1108	19	0.6395192321078085
1108	20	0.6632333777334267
1108	21	0.5922480728264858
1108	22	0.5721011974395722
1108	23	0.8701410591400744
1108	24	0.828566560071998
1108	25	0.7842208146169156
1109	1	0.2238681999446861
1109	2	0.11484821935491851
1109	3	0.2973422504363654
1109	4	0.535374031624535
1109	5	0.43557816348657497
1109	6	0.28638813222113413
1109	7	0.18974714671031023
1109	8	0.21316096379623417
1109	9	0.52520651804641
1109	10	0.38423818134815946
1109	11	0.18432890771588717
1109	12	0.23292554569363233
1109	13	0.013757462417309796
1109	14	0.4968130370230668
1109	15	0.2314120551520631
1109	16	0.32727295536967205
1109	17	0.6436837124531607
1109	18	0.3287023487369751
1109	19	0.18387562846274363
1109	20	0.2537297349473633
1109	21	0.13826042814956452
1109	22	0.26519709870444075
1109	23	0.4776825867717278
1109	24	0.3788431412275841
1109	25	0.3725491723862646
1110	1	0.37242917467011694
1110	2	0.4570767577239323
1110	3	0.5267604135096065
1110	4	0.6811069279771971
1110	5	0.5662677007000809
1110	6	0.47785219087617037
1110	7	0.42868319651223885
1110	8	0.49987545872255623
1110	9	1.077046581859463
1110	10	0.9970580151000306
1110	11	0.573644793350546
1110	12	0.6232922658005791
1110	13	0.6156389069757822
1110	14	0.9652304639131373
1110	15	0.5957934011849559
1110	16	0.6285678180092376
1110	17	0.9307418646176444
1110	18	1.074660829267529
1110	19	0.6842249607745572
1110	20	0.7913950411727626
1110	21	0.7165085013832382
1110	22	0.811966326563379
1110	23	0.8373383304980138
1110	24	0.6574455912829933
1110	25	0.7638803397331557
1111	1	0.19480189602005638
1111	2	0.0522569073184016
1111	3	0.12879406119041903
1111	4	0.429340031386495
1111	5	0.24080861004443221
1111	6	0.29415001417199704
1111	7	0.10480055324379833
1111	8	0.08051509613965635
1111	9	0.531723413963995
1111	10	0.42938106766431616
1111	11	0.058113502321091914
1111	12	-0.01738259727228808
1111	13	-0.028947668933142268
1111	14	-0.05654345184010623
1111	15	0.020801478186463385
1111	16	0.012764391222006566
1111	17	0.25338679810363374
1111	18	0.3566459461988316
1111	19	0.19022974358150435
1111	20	-0.2184456108532089
1111	21	-0.31761379396693457
1111	22	-0.10103898248981125
1111	23	0.2281455189147934
1111	24	0.14681121632834504
1111	25	0.30108297050610844
1112	1	0.02946269284587899
1112	2	0.08569985947067593
1112	3	0.18919643312964496
1112	4	0.46453734344950703
1112	5	0.3017511918302205
1112	6	0.3256235946360633
1112	7	0.24242163772755987
1112	8	0.279010955460528
1112	9	-1.1579055639440483
1112	10	0.1477823594736165
1112	11	0.10282131848457254
1112	12	0.08856126857137525
1112	13	-0.010509612138299929
1112	14	0.4811226199461558
1112	15	0.3452655515512646
1112	16	0.04807304866977802
1112	17	0.3466995188972959
1112	18	0.45764048433462534
1112	19	0.3891538878176064
1112	20	0.2588666984623842
1112	21	0.4101209504657106
1112	22	0.39201953468957385
1112	23	0.029631983423541897
1112	24	0.17247817482085992
1112	25	0.37171694736554173
1113	1	0.18762289459921178
1113	2	0.22928631851288017
1113	3	0.2413790662898201
1113	4	0.45811434231051607
1113	5	0.39779820465398785
1113	6	0.3676559494821561
1113	7	0.2487816166106569
1113	8	0.22485643050018922
1113	9	0.07781874892689444
1113	10	0.4510167150197333
1113	11	0.2833252707190468
1113	12	0.13783639287952099
1113	13	0.2877320258655587
1113	14	-0.09560718188271977
1113	15	0.12469559359689697
1113	16	0.2417199810221291
1113	17	0.5537189391267532
1113	18	0.39308721872455976
1113	19	0.38733000594088907
1113	20	0.3753184838705792
1113	21	0.5166633243792802
1113	22	0.5727867297236173
1113	23	0.40347747351738406
1113	24	0.35640619943580276
1113	25	0.36551175278518144
1114	1	0.24344915103802478
1114	2	0.21157474643683827
1114	3	0.3036259907113248
1114	4	0.5542135524985525
1114	5	0.3928603429014217
1114	6	0.39964148989036374
1114	7	0.17962755587926438
1114	8	0.09041764598483581
1114	9	0.09674738424143925
1114	10	0.3026773905701461
1114	11	0.3007845852687083
1114	12	0.27416353916072467
1114	13	0.2506936006188596
1114	14	0.7104311731602238
1114	15	0.37938090034958094
1114	16	0.21752784963244665
1114	17	0.6591748807235831
1114	18	0.45185429816545053
1114	19	0.45785641107679
1114	20	0.46256389117723984
1114	21	0.45488363166543877
1114	22	0.17970425777437526
1114	23	0.7521257341105141
1114	24	0.4434086700523636
1114	25	0.3994282928272952
1115	1	0.18269285100484467
1115	2	0.24875035379414
1115	3	0.23456845842472873
1115	4	0.5844276566730429
1115	5	0.5364388389009491
1115	6	0.31913817161464664
1115	7	0.40990338230184165
1115	8	0.39093418533013846
1115	9	0.4288977291798659
1115	10	0.23755862458448718
1115	11	0.32757685978609885
1115	12	0.30865372889896747
1115	13	0.20953469107771383
1115	14	0.24389319276703958
1115	15	0.2634676462529555
1115	16	0.2032153625526547
1115	17	0.6142636147856635
1115	18	0.6221635514915747
1115	19	0.5654217183032486
1115	20	0.6155894411889875
1115	21	0.41566193691041065
1115	22	0.022100483079547928
1115	23	0.3985771470001822
1115	24	0.4927965291477534
1115	25	0.48669331157658036
1116	1	0.4488893584065998
1116	2	0.4032615953466987
1116	3	0.4561572300051928
1116	4	0.7899255033423032
1116	5	0.6729174805062215
1116	6	0.6726485923687662
1116	7	0.5274371667052478
1116	8	0.6005100771042393
1116	9	0.8114275856385902
1116	10	0.6511920859744791
1116	11	0.4723904607615097
1116	12	0.4960264740112095
1116	13	0.41561386251846955
1116	14	0.8023380468712062
1116	15	0.5887648920175224
1116	16	0.6577873496914961
1116	17	1.0338322689835522
1116	18	1.133975019470654
1116	19	0.6546490900202926
1116	20	0.5876278239590533
1116	21	0.693721039673008
1116	22	0.7046012825007892
1116	23	1.0230114717467131
1116	24	0.7925773162812391
1116	25	0.7420052993251431
1201	1	-0.05142788187958658
1201	2	0.1923720704661953
1201	3	-6.7308075272976E-4
1201	4	0.14792151388352437
1201	5	-0.046642262819112545
1201	6	-0.1179695361433275
1201	7	-0.22761878685308545
1201	8	-0.035790543198608304
1201	9	0.3820113218334811
1201	10	0.49953004856185856
1201	11	0.36731741421214537
1201	12	0.25736376960459495
1201	13	0.3588596612926196
1201	14	0.76294288396101
1201	15	0.45238908443818104
1201	16	0.2474321546805598
1201	17	0.32444977223762805
1201	18	0.45128994583323845
1201	19	0.16614602180104754
1201	20	0.1785016517370437
1201	21	0.11040923866262631
1201	22	0.08802051846988235
1201	23	0.04233069394449984
1201	24	-0.12752385359213747
1201	25	0.010158923344057769
1202	1	-0.202562397815381
1202	2	-0.23650857307818995
1202	3	-0.35120651514926493
1202	4	-0.17995440658625483
1202	5	-0.30291775784541386
1202	6	-0.42943450429619645
1202	7	-0.4044378225960443
1202	8	-0.3780480017036112
1202	9	0.1045531020943713
1202	10	0.23763250545717796
1202	11	-0.09761915640373076
1202	12	-0.06755279212749343
1202	13	0.01223773954324514
1202	14	-0.18174538904003867
1202	15	-0.05877697299668938
1202	16	-0.12652328785808464
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2211	6	-0.00976566587895178
2211	7	0.09987425674663086
2211	8	0.08318286527796914
2211	9	-1.4821559058964908
2211	10	-0.12908710956487823
2211	11	-0.16465220832957073
2211	12	-0.06236593651332356
2211	13	-0.24880511737641342
2211	14	0.17184372825011707
2211	15	-0.025034722118704167
2211	16	0.012247233969659987
2211	17	0.28249505383909224
2211	18	0.3214347864834082
2211	19	0.01947361110165957
2211	20	0.23897041293542287
2211	21	0.059151332116677224
2211	22	0.06979890866547578
2211	23	-1.8882591052183813
2211	24	-0.6211876309789588
2211	25	-0.4536507932784275
2212	1	-0.09695358777356233
2212	2	-1.411396239396229
2212	3	-0.44724304049944763
2212	4	0.29975124065260417
2212	5	0.028615783066300082
2212	6	0.299660133300371
2212	7	0.2242757791513128
2212	8	0.1784642706976527
2212	9	0.7310305574732894
2212	10	0.5874829839772673
2212	11	0.29226088406207396
2212	12	0.21836480073929465
2212	13	0.20575687777915164
2212	14	0.46795496888717025
2212	15	0.1990672987130253
2212	16	0.3272886967977797
2212	17	0.5255163097416471
2212	18	0.7408530126069905
2212	19	0.3981734231266856
2212	20	0.3445408169735842
2212	21	0.26678424569074366
2212	22	0.3640548641790673
2212	23	0.5663909333940254
2212	24	0.36175471835594664
2212	25	0.17774436077161937
2213	1	0.12113784258070481
2213	2	0.04409939372378702
2213	3	0.0195715388765052
2213	4	0.1610569023959343
2213	5	0.1786360795244093
2213	6	0.1868612569333976
2213	7	0.18673021278710422
2213	8	0.056214610863790426
2213	9	0.36767369339305134
2213	10	0.4141345696384917
2213	11	0.11981548333945824
2213	12	0.16518912185973988
2213	13	-0.1081046068607705
2213	14	-1.539254352473833
2213	15	-0.5791228570570013
2213	16	-0.2664802107223707
2213	17	0.3248364426131545
2213	18	0.09203845836295699
2213	19	0.04029254136273863
2213	20	0.11135058518296859
2213	21	0.2374774375984572
2213	22	0.27474065952564786
2213	23	0.4476114923349428
2213	24	0.24825950720231305
2213	25	-0.025828940544663226
2214	1	0.09242673210761154
2214	2	0.17541855600377332
2214	3	0.2043092168314935
2214	4	0.32213729172730154
2214	5	0.2903460416321053
2214	6	0.37298126681359633
2214	7	0.05427585519181832
2214	8	0.011591735206430087
2214	9	0.12323721392221643
2214	10	0.08252860245820415
2214	11	-0.1383080708735136
2214	12	-0.07524773506568039
2214	13	-0.03619312140907027
2214	14	0.01792306955219658
2214	15	-0.14407411820111093
2214	16	0.012072710589741575
2214	17	0.040890569811004696
2214	18	0.2866812899887776
2214	19	-0.021379797109979393
2214	20	0.0699189523685746
2214	21	0.2617774921291911
2214	22	0.13894393238349068
2214	23	0.18334626740605842
2214	24	0.2631249751323992
2214	25	0.3140065506299621
2215	1	0.2882600118150762
2215	2	0.18625616772086317
2215	3	0.29478191396546194
2215	4	0.6369859326365201
2215	5	0.3180395921146655
2215	6	0.4934346336632558
2215	7	0.4910795793306235
2215	8	0.40527597188430775
2215	9	0.6553977191207849
2215	10	0.6632122896870456
2215	11	0.33573505636168477
2215	12	0.37485949425831677
2215	13	0.22633959597275322
2215	14	0.24041432821564968
2215	15	0.239798665745937
2215	16	0.356787726082338
2215	17	0.5901294570745748
2215	18	0.7587448383384725
2215	19	0.45626135369665377
2215	20	-0.320445603001275
2215	21	0.16028482331351768
2215	22	-0.4686189605140285
2215	23	0.3459827340331927
2215	24	0.1944795107570414
2215	25	0.3834575235568707
2216	1	-0.0777255382332811
2216	2	-0.49144230167625835
2216	3	-0.07494942022012907
2216	4	0.08586498158641831
2216	5	-0.0843920661581592
2216	6	0.1586366433564308
2216	7	0.004504677630436049
2216	8	0.00765905464660932
2216	9	0.2143116948852679
2216	10	-0.008406048473652561
2216	11	-0.06629135427766641
2216	12	-0.5180615028418174
2216	13	-0.194037036089874
2216	14	-0.21200563275885997
2216	15	-0.20974347649691083
2216	16	-0.09365810791162943
2216	17	-0.037475255304322275
2216	18	0.4037525928033716
2216	19	0.2353462024122095
2216	20	-1.0558458778970206
2216	21	-1.2086211090954393
2216	22	-1.2207097216933889
2216	23	-1.679531728422667
2216	24	-1.2432254460516532
2216	25	-1.11565356800741
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4213.0
3	535.0
4	498.0
5	396.0
6	464.0
7	609.0
8	290.0
9	606.0
10	1450.0
11	3306.0
12	6698.0
13	11103.0
14	16367.0
15	21991.0
16	27394.0
17	29731.0
18	28838.0
19	27311.0
20	25557.0
21	23868.0
22	22304.0
23	21739.0
24	21200.0
25	21261.0
26	22094.0
27	23290.0
28	25563.0
29	30032.0
30	36504.0
31	46059.0
32	59263.0
33	79123.0
34	107643.0
35	152448.0
36	233131.0
37	381411.0
38	673971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13017632334031	21.204471922350002	12.719717736215157	19.945634018094534
2	13.852974736620215	25.475733316225295	37.25993373689975	23.411358210254743
3	19.621410777651278	32.45248950108187	28.7890674613091	19.13703225995775
4	12.266130478473663	23.168797002654188	31.61605427437786	32.94901824449429
5	10.472877824758337	45.15044736521019	30.974249781431844	13.402425028599632
6	26.993317096788395	37.01276610572338	20.69013263105386	15.303784166434367
7	21.88767191623674	39.884200120678365	19.571903582020024	18.656224381064867
8	22.147204434604443	38.54901342157541	21.69688370719431	17.606898436625844
9	25.933226589781967	23.849984960530563	17.538268113390263	32.678520336297204
10	15.363876456391058	32.34314183260573	26.841306695018574	25.45167501598464
11	27.49342129986499	32.006075043468456	19.209987708760874	21.29051594790568
12	23.51837825346988	31.931825684439097	23.879808679832156	20.66998738225887
13	27.111178134458104	26.208608945202762	27.203768138129618	19.47644478220952
14	25.44406284266231	29.556789802003074	22.71500397900844	22.28414337632618
15	28.2890591393658	28.944986145875085	22.90337699453998	19.862577720219136
16	21.94760073520312	30.07040349083975	26.58543069427192	21.3965650796852
17	19.242535484223044	34.9684515896189	24.50398212982839	21.285030796329664
18	19.201792315817272	28.326805152128625	32.38312538508934	20.08827714696476
19	21.753028965738586	33.92803842230144	23.681800988067536	20.637131623892436
20	21.574385312960334	30.891787764650235	27.59186962208996	19.94195730029947
21	23.134347520273497	32.41668905776721	23.97550398317045	20.473459438788844
22	21.492184485197132	31.25064735909571	22.526767439281155	24.73040071642601
23	24.40155781240907	28.020781829737647	24.02864695863201	23.549013399221277
24	20.66715592595835	30.565809576677644	22.65296496173097	26.114069535633032
25	23.299244658776356	31.820542402745062	23.79198803848257	21.08822489999601
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53546.0
1	53546.0
2	35953.0
3	18360.0
4	18360.0
5	18360.0
6	19977.5
7	21595.0
8	21595.0
9	21595.0
10	20880.0
11	20165.0
12	20165.0
13	20165.0
14	21766.5
15	23368.0
16	23368.0
17	23368.0
18	28868.0
19	34368.0
20	34368.0
21	34368.0
22	44881.0
23	55394.0
24	55394.0
25	55394.0
26	68904.5
27	82415.0
28	82415.0
29	82415.0
30	93102.5
31	103790.0
32	103790.0
33	103790.0
34	112062.5
35	120335.0
36	120335.0
37	120335.0
38	144497.0
39	168659.0
40	168659.0
41	168659.0
42	310822.0
43	452985.0
44	452985.0
45	452985.0
46	381502.5
47	310020.0
48	310020.0
49	310020.0
50	280357.0
51	250694.0
52	250694.0
53	250694.0
54	212801.5
55	174909.0
56	174909.0
57	174909.0
58	147671.5
59	120434.0
60	120434.0
61	120434.0
62	106988.0
63	93542.0
64	93542.0
65	93542.0
66	68704.5
67	43867.0
68	43867.0
69	43867.0
70	33994.5
71	24122.0
72	24122.0
73	24122.0
74	17022.0
75	9922.0
76	9922.0
77	9922.0
78	6719.0
79	3516.0
80	3516.0
81	3516.0
82	2374.5
83	1233.0
84	1233.0
85	1233.0
86	956.5
87	680.0
88	680.0
89	680.0
90	485.5
91	291.0
92	291.0
93	291.0
94	166.5
95	42.0
96	42.0
97	42.0
98	25.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14033974923466627
2	0.2765209451706172
3	0.3346035961889372
4	0.362479612806699
5	0.37303594041113014
6	0.3277945363921397
7	0.3316332009755692
8	0.3469878593092871
9	0.3351062784558149
10	0.3675521338633737
11	0.2841982743374762
12	0.3292568848048747
13	0.3016093601266028
14	0.31134311674887044
15	0.3511007142201044
16	0.29937013911960225
17	0.34931847709208363
18	0.3175580975029944
19	0.3306735348297118
20	0.29333795191707024
21	0.33519767523161087
22	0.28876811312727324
23	0.27281937575088167
24	0.30764154732913485
25	0.3015179633508069
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2188261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.57275425039759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87354324099223	14.834716897234543
2	7.027312325047608	2.610330897077987
3	2.7668879779996396	1.541661913613004
4	1.5970109637893242	1.1864356866259889
5	1.0631471844384126	0.9872785694288381
6	0.7761951610653585	0.8649649186088808
7	0.6285849240077481	0.8172187323370526
8	0.5008912006274538	0.7442343340352237
9	0.4430722150914083	0.7406164229464827
>10	4.049516769277582	16.182295331402507
>50	0.7257167164606714	9.43435319220261
>100	0.47545184292646775	16.410818262852654
>500	0.03745082327542837	4.720490600419155
>1k	0.028522150176650746	10.435132358056478
>5k	0.003472261760635743	4.797739765957791
>10k+	0.0032242430634474755	13.691712117200803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	66646	3.0456147598481165	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	47085	2.1517085941759233	No Hit
GCTTATGTACTCTGCGTTGATACCA	40010	1.8283924997977845	No Hit
CTTATGTACTCTGCGTTGATACCAC	27359	1.2502621945005647	No Hit
GAGTACATAAGCAGTGGTATCAACG	18310	0.836737482411833	No Hit
TATGTACTCTGCGTTGATACCACTG	15866	0.725050622389194	No Hit
GTATCAACGCAGAGTACTTTTTTTT	14681	0.6708980327300993	No Hit
GCGTTGATACCACTGCTTATGTACT	13485	0.6162427608041271	No Hit
CATAAGCAGTGGTATCAACGCAGAG	12177	0.5564692694335822	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10502	0.4799244697044822	No Hit
ACTCTGCGTTGATACCACTGCTTAT	10423	0.47631429706054257	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	10419	0.4761315035089507	No Hit
CTGCTTATGTACTCTGCGTTGATAC	10270	0.4693224437121532	No Hit
CCCATGTACTCTGCGTTGATACCAC	9311	0.42549768971799984	No Hit
GTGGTATCAACGCAGAGTACATGGG	9129	0.41718058312056927	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9083	0.41507845727726267	No Hit
GTATCAACGCAGAGTACATAAGCAG	8747	0.39972379894354465	No Hit
TATCAACGCAGAGTACATAAGCAGT	8517	0.38921316972701153	No Hit
GGTATCAACGCAGAGTACATAAGCA	7913	0.3616113434366376	No Hit
GTACATGATAAGCAGTGGTATCAAC	7682	0.3510550158322065	No Hit
ATCATGTACTCTGCGTTGATACCAC	7638	0.3490442867646958	No Hit
CATGTACTCTGCGTTGATACCACTG	7303	0.3337353268188758	No Hit
GTACTGGTTCACTATCGGTCAGTCA	6483	0.29626264874254027	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	5924	0.270717249907575	No Hit
GTGGTATCAACGCAGAGTACATAAG	5626	0.2570991303139799	No Hit
ACATAAGCAGTGGTATCAACGCAGA	5610	0.2563679561076124	No Hit
ATACAGGGTGACAGCCCCGTACACA	5188	0.237083236414669	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5000	0.2284919394898506	No Hit
GGATACCACGTGTCCCGCCCTACTC	4424	0.2021696680606198	No Hit
CCACTGCTTATGTACTCTGCGTTGA	4395	0.2008444148115787	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4168	0.19047088075873947	No Hit
GATACCACTGCTTATGTACTCTGCG	4077	0.1863123274600242	No Hit
GTACTCTGCGTTGATACCACTGCTT	3858	0.17630438051036873	No Hit
GCAGTGGTATCAACGCAGAGTACAT	3818	0.17447644499444995	No Hit
GGTATCAACGCAGAGTACATGGGGG	3802	0.1737452707880824	No Hit
ATACCACTGCTTATGTACTCTGCGT	3726	0.1702721933078367	No Hit
GTTGATACCACTGCTTATGTACTCT	3571	0.1631889431836513	No Hit
GTATCAACGCAGAGTACATGGGGGT	3460	0.15811642212697663	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3312	0.15135306071807705	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3305	0.15103317200279126	No Hit
GTTAATGATAGTGTGTCGAAACACA	3232	0.14769718968623943	No Hit
GAGTACATGATAAGCAGTGGTATCA	3100	0.1416650024837074	No Hit
CCCATATTCAGACAGGATACCACGT	3059	0.1397913685798906	No Hit
GGTATCAACGCAGAGTACATGGGGA	2979	0.136135497548053	No Hit
CAGTGGTATCAACGCAGAGTACATA	2953	0.13494733946270576	No Hit
TACCACTGCTTATGTACTCTGCGTT	2916	0.13325649911048087	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2863	0.13083448455188845	No Hit
TGATACCACTGCTTATGTACTCTGC	2856	0.13051459583660266	No Hit
GCTTATCATGTACTCTGCGTTGATA	2776	0.12685872480476507	No Hit
ATCATTAACTGAATCCATAGGTTAA	2746	0.12548777316782597	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	2580	0.11790184077676291	No Hit
GCAGAGTACATAAGCAGTGGTATCA	2575	0.11767334883727307	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	2533	0.11575401654555831	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	2450	0.1119610503500268	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	2430	0.1110470825920674	No Hit
TATTCAGACAGGATACCACGTGTCC	2390	0.10921914707614859	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	2342	0.10702562445704604	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	2331	0.10652294219016835	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	2303	0.10524338732902519	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2260	0.10327835664941248	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	2259	0.1032326582615145	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	2249	0.10277567438253479	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	2228	0.10181600823667744	No Hit
GCGTTGATACCACTGCTTATCATGT	2211	0.10103913564241195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.569838789797012E-5	0.0
2	0.0	0.0	0.0	4.569838789797012E-5	0.0
3	0.0	0.0	0.0	4.569838789797012E-5	0.0
4	0.0	0.0	0.0	4.569838789797012E-5	0.0
5	1.8279355159188048E-4	0.0	0.0	4.569838789797012E-5	0.0
6	2.2849193948985063E-4	0.0	0.0	4.569838789797012E-5	0.0
7	2.2849193948985063E-4	0.0	0.0	4.569838789797012E-5	0.0
8	2.7419032738782074E-4	0.0	0.0	4.569838789797012E-5	0.0
9	2.7419032738782074E-4	0.0	0.0	1.3709516369391037E-4	0.0
10	3.198887152857909E-4	0.0	0.0	1.3709516369391037E-4	0.0
11	3.198887152857909E-4	0.0	0.0	1.3709516369391037E-4	0.0
12	3.198887152857909E-4	0.0	0.0	1.3709516369391037E-4	0.0
13	3.6558710318376097E-4	0.0	0.0	1.3709516369391037E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCC	65	1.9019171E-7	16.08611	4
CTAGTTC	70	4.4108856E-7	14.936759	3
CTAATAG	80	1.2806049E-7	14.257817	3
CCGTCTT	110	1.8735591E-10	13.815296	15
ATCGATT	55	1.9645807E-4	13.814979	16
GCCTAGT	70	7.2144467E-6	13.580743	1
GACTGTC	50	0.0015012099	13.298442	7
CACGCAT	115	3.8016879E-10	13.213721	17
TAGTCAG	65	5.4651515E-5	13.151098	15
ACATAAT	125	1.0186341E-10	12.927087	3
GAACGCA	75	1.4705669E-5	12.673905	4
GATACAC	60	4.074311E-4	12.673614	3
TATTGTA	90	5.372458E-7	12.673614	2
ATAAGAC	90	5.372458E-7	12.673614	3
GTGGGCA	105	2.0008883E-8	12.6640215	13
CGATAGT	90	5.431066E-7	12.662278	18
TCTAGGA	145	7.2759576E-12	12.455104	2
CATAATC	100	1.4327452E-7	12.357057	4
CGAATGG	355	0.0	12.305594	19
CGCAAGT	85	3.957637E-6	12.290705	17
>>END_MODULE