FastQCFastQC Report
Wed 25 May 2016
SRR1295255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA718453.283200678529664No Hit
GTATCAACGCAGAGTACTTTTTTTT473912.1656923008727023No Hit
GCTTATGTACTCTGCGTTGATACCA429931.9647107908974295No Hit
GGTATCAACGCAGAGTACTTTTTTT342061.5631590564379663No Hit
CTTATGTACTCTGCGTTGATACCAC322091.4718993758057197No Hit
TATCAACGCAGAGTACTTTTTTTTT288811.319815140881275No Hit
GAGTACATAAGCAGTGGTATCAACG193930.8862288365053347No Hit
ACGCAGAGTACTTTTTTTTTTTTTT155160.7090561866249044No Hit
CATAAGCAGTGGTATCAACGCAGAG150030.6856129136332457No Hit
GCGTTGATACCACTGCTTATGTACT147760.6752393795804066No Hit
GTACTTTTTTTTTTTTTTTTTTTTT147390.6735485392281817No Hit
TATGTACTCTGCGTTGATACCACTG139710.6384521773225407No Hit
CTGCTTATGTACTCTGCGTTGATAC119600.5465527192597227No Hit
ACGCAGAGTACATAAGCAGTGGTAT119560.5463699257081308No Hit
ACTCTGCGTTGATACCACTGCTTAT112000.5118219444572654No Hit
CCCATGTACTCTGCGTTGATACCAC108160.49427376350444485No Hit
GTATCAACGCAGAGTACATAAGCAG97360.44491950457463714No Hit
GTGGTATCAACGCAGAGTACATGGG94660.4325809398421852No Hit
TATCAACGCAGAGTACATAAGCAGT86100.39346311980152276No Hit
CATGTACTCTGCGTTGATACCACTG82730.3780627630799068No Hit
GGTATCAACGCAGAGTACATAAGCA81620.37299024202323217No Hit
ATCATGTACTCTGCGTTGATACCAC80950.36992845003406816No Hit
GTACATGATAAGCAGTGGTATCAAC80760.3690601806640067No Hit
GAGTACTTTTTTTTTTTTTTTTTTT77770.3553963626825136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70730.3232246976023427No Hit
GTACTGGTTCACTATCGGTCAGTCA68110.3112517199730745No Hit
ACATAAGCAGTGGTATCAACGCAGA64910.29662823584572406No Hit
AAGCAGTGGTATCAACGCAGAGTAC64340.29402342773553974No Hit
ATACAGGGTGACAGCCCCGTACACA59890.27368764512094307No Hit
GCAGAGTACTTTTTTTTTTTTTTTT57810.26418238043816533No Hit
GTGGTATCAACGCAGAGTACATAAG56500.2581958916235312No Hit
CCACTGCTTATGTACTCTGCGTTGA48630.2222312603478287No Hit
GGATACCACGTGTCCCGCCCTACTC48000.21935226191025659No Hit
GTGGTATCAACGCAGAGTACTTTTT47890.21884957964337892No Hit
GATACCACTGCTTATGTACTCTGCG44860.20500296811029398No Hit
ATCAACGCAGAGTACTTTTTTTTTT44040.2012557003026604No Hit
GCAGTGGTATCAACGCAGAGTACAT43430.19846809864088427No Hit
GGTATCAACGCAGAGTACATGGGGG39950.18256505965239064No Hit
ATACCACTGCTTATGTACTCTGCGT38480.17584739663138904No Hit
GTACTCTGCGTTGATACCACTGCTT38250.1747963337097357No Hit
GTATCAACGCAGAGTACATGGGGGT36760.1679872739129382No Hit
CCCATATTCAGACAGGATACCACGT35140.160584135073467No Hit
GTTAATGATAGTGTGTCGAAACACA34790.15898469149703806No Hit
GTACATGGGAAGCAGTGGTATCAAC34390.15715675598111925No Hit
CAGTGGTATCAACGCAGAGTACATA34230.15642558177475174No Hit
GTTGATACCACTGCTTATGTACTCT34220.15637988338685374No Hit
GAGTACATGATAAGCAGTGGTATCA33100.15126166394228113No Hit
GGTATCAACGCAGAGTACATGGGGA33070.15112456877858718No Hit
ACCCTGTATCGCGCGCCTTTCCAGA32120.14678322192828003No Hit
GCTTATCATGTACTCTGCGTTGATA29500.13481024429901187No Hit
TACCACTGCTTATGTACTCTGCGTT29250.13366778460156262No Hit
GCAGAGTACATAAGCAGTGGTATCA28290.12928073936335746No Hit
ATCATTAACTGAATCCATAGGTTAA28070.12827537482960213No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG27280.1246652021856625No Hit
CTATCGGTCAGTCAGGAGTATTTAG26910.1229743618334376No Hit
GCCCAGAGCCTGAATCAGTGTGTGT26640.1217405053601924No Hit
GCCTTGGAGGATGGTCCCCCCATAT26400.12064374405064111No Hit
TGATACCACTGCTTATGTACTCTGC25260.11543412783027254No Hit
ACTTAGATGTTTCAGTTCCCCCGGT25250.11538842944237455No Hit
GTTCACTATCGGTCAGTCAGGAGTA25160.11497714395129284No Hit
GTATTTAGCCTTGGAGGATGGTCCC24090.11008741644621003No Hit
GCGTTGATACCACTGCTTATCATGT23350.10670573574176025No Hit
ACGCAGAGTACATGATAAGCAGTGG23210.10606595831118865No Hit
CGGTACTGGTTCACTATCGGTCAGT23030.10524338732902519No Hit
CAACGCAGAGTACTTTTTTTTTTTT22950.10487780022584144No Hit
GATAAGCAGTGGTATCAACGCAGAG22870.10451221312265768No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA22750.10396383246788203No Hit
TCTAAGTACCCCGAGGAAAAGAAAT22640.10346115020100435No Hit
TATTCAGACAGGATACCACGTGTCC22590.1032326582615145No Hit
GGTTAATGAGGCGAACCGGGGGAAC22450.10259288083094294No Hit
GTCAGGAGTATTTAGCCTTGGAGGA22340.10209019856406525No Hit
ATCAACGCAGAGTACATAAGCAGTG22180.10135902435769774No Hit
CCCCATGTACTCTGCGTTGATACCA21950.10030796143604442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGT401.2840821E-519.0026051
TCGTAGT350.002168592516.2879491
TACTGAC350.00216958616.286832
GATACAC651.912831E-716.0780263
AGCCGAT551.1272621E-515.5475861
CGTAGTT551.1279948E-515.546522
CTAGTTC508.711436E-515.2010433
AGTATGA508.723329E-515.1986115
GGATACA704.4349326E-714.9295962
TAAGCTC801.2877899E-714.2513025
CCGAACC400.00527761314.25097753
GTTAGAG602.5675454E-514.2509772
GGTCCAT602.5692058E-514.2499998
CGGCTGC1400.014.24967514
ACTGGGC1350.014.0740748
CTATAAG759.662945E-713.9352441
TTAAGCT759.670421E-713.9342884
CCGATAC551.959587E-413.8191293
CTTGCAC1101.8553692E-1013.8191293
AGGCCGT903.6425263E-813.72190956
Produced by FastQC (version 0.11.5)