FastQCFastQC Report
Wed 25 May 2016
SRR1295254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4731785
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG67510.14267343085114814No Hit
GTATCAACGCAGAGTACTTTTTTTT66270.14005285531781347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN59400.12553402151619314No Hit
GGTATCAACGCAGAGTACTTTTTTT58070.12272324291995515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18700.010.8796741
GGTATCA38300.010.5495491
TGGTATC18450.010.5105832
ACACCGC1054.115054E-59.9510536
ACGGCAG800.0044948729.5059955
CCTACAC5000.08.7455163
GCGTCAG2201.5861588E-98.6427321
TACACCG1356.753873E-58.4497745
ACTAGAC3400.08.3876443
TATACAG9600.08.3177465
CGTCAGA2409.240466E-108.3176582
GTATCAA67200.08.2620411
TATTCCG2105.7547368E-88.1479965
GTCTTGG6350.08.0846971
CTAGTAC2951.2732926E-118.0559293
GTCTCGC2605.18412E-108.044391
GTCTAGA4750.08.0058981
TCTGGAC5400.07.9216633
GGACACG1200.00169412957.915616
CAGTGCG1451.5660496E-47.8609369
Produced by FastQC (version 0.11.5)